Standard name
Human Ortholog
Description High-affinity cysteine-specific transporter; has similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.94 0.97 0.96 0.92 0.87 – 0.85 0.73 0.76 0.67 0.71 0.99 0.99 1.0 0.77 0.77 0.75 0.82 0.79 0.52 0.73 0.76 0.78
Endoplasmic Reticulum – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.11 0 0 0.05 0.21 – 0.13 0.33 0.34 0.4 0.35 0 0 0 0.05 0 0 0.12 0 0.21 0.13 0.1 0.07
Nucleus – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.22 0.3 0.33 0 0.09 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 – 0 0 0 0 0 3 3 0 0 0 0 0 0 17 9 11 12
Bud – 0 0 2 3 7 – 16 31 15 30 38 3 3 0 0 0 1 0 0 11 3 10 17
Bud Neck – 0 3 2 2 6 – 7 5 8 7 5 46 50 25 0 0 0 0 0 9 3 5 11
Bud Site – 0 0 0 0 0 – 1 1 3 1 5 0 0 0 0 0 0 – – – – – –
Cell Periphery – 1 5 4 1 4 – 8 7 2 6 8 17 17 6 0 0 0 0 0 6 12 12 21
Cytoplasm – 44 606 492 678 691 – 915 658 868 519 688 1317 1317 1323 68 183 117 19 51 317 364 456 635
Endoplasmic Reticulum – 0 3 2 0 0 – 0 2 2 5 0 1 0 1 3 9 11 0 0 16 7 3 2
Endosome – 0 4 5 24 8 – 21 11 13 11 7 1 1 0 0 3 3 0 0 20 1 2 3
Golgi – 0 2 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 5 0 0 0
Mitochondria – 5 5 12 36 171 – 143 292 383 312 339 0 0 0 4 4 4 2 2 130 65 59 59
Nucleus – 0 1 0 1 1 – 2 2 2 5 8 2 2 2 11 8 3 0 0 1 0 0 1
Nuclear Periphery – 0 0 0 0 1 – 2 1 2 3 6 0 0 0 0 1 0 0 0 0 0 1 0
Nucleolus – 0 2 1 0 0 – 3 2 0 2 1 0 0 0 0 1 0 0 0 0 0 0 1
Peroxisomes – 0 1 6 9 4 – 6 0 2 5 2 0 0 0 0 0 0 0 0 1 0 0 1
SpindlePole – 0 5 3 18 12 – 39 45 46 26 30 3 3 1 0 0 0 0 0 7 7 10 15
Vac/Vac Membrane – 0 4 4 12 15 – 20 17 28 15 31 2 3 0 19 72 51 0 5 48 9 10 8
Unique Cell Count 47 627 510 739 797 1081 898 1136 772 969 1328 1328 1324 88 238 155 24 65 613 502 602 817
Labelled Cell Count 50 641 533 784 920 1183 1074 1374 947 1168 1395 1399 1358 105 282 190 24 65 613 502 602 817


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 3.0 4.0 4.1 3.9 3.1 – 3.5 3.2 2.9 3.3 3.1 5.8 5.8 5.9 5.1 6.0 6.8 5.1 5.3 5.3
Std Deviation (1e-4) – 0.4 1.4 1.9 1.7 1.1 – 1.3 1.2 0.9 1.2 1.0 0.9 0.9 0.9 1.2 1.5 1.8 1.3 1.2 1.4
Intensity Change (Log2) – – – 0.06 -0.01 -0.34 – -0.17 -0.31 -0.44 -0.27 -0.38 0.55 0.55 0.58 0.35 0.59 0.77 0.36 0.41 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 13.7 14.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5026 0.5756 0.2708 -0.1914 0.1908 0.4702 -1.654 -0.7481 -1.1256 -1.6637 -1.8638 -1.2195 -2.5186 -2.0213 -2.3353 -2.1022 -2.5791 -2.2207
Actin 0.013 0.0002 0.0004 0.0001 0.0009 0.0007 0.0001 0.0002 0.0005 0.0003 0.0005 0.0007 0.0204 0.0005 0.0011 0.0001 0.0001 0.0033
Bud 0.0009 0.0043 0.0023 0.0002 0.0006 0.0055 0 0 0.0001 0 0 0.0014 0.0002 0.0001 0.0003 0 0 0
Bud Neck 0.0027 0.0003 0.0002 0 0.0041 0.0007 0 0.0001 0 0 0.0001 0.0002 0.0074 0.0001 0.0001 0 0 0.0002
Bud Periphery 0.0026 0.0013 0.0018 0.0005 0.0005 0.0026 0 0 0.0001 0.0001 0.0001 0.0017 0.0006 0.0002 0.0013 0 0 0
Bud Site 0.0025 0.0117 0.0012 0.0001 0.0001 0.0002 0.0001 0.0003 0.0002 0 0.0001 0 0.0014 0.0004 0.0002 0 0 0.0001
Cell Periphery 0.0003 0.0005 0.0002 0 0 0.0002 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0006 0.0001 0.0003 0 0 0
Cytoplasm 0.0452 0.0711 0.0572 0.0807 0.0689 0.0835 0.1338 0.1343 0.0898 0.0556 0.1301 0.1449 0.0365 0.0804 0.0708 0.0509 0.0887 0.1553
Cytoplasmic Foci 0.0147 0.0091 0.0086 0.0071 0.0093 0.0125 0.0069 0.0047 0.0094 0.0042 0.0061 0.0051 0.0095 0.0075 0.0092 0.0051 0.013 0.0063
Eisosomes 0.0004 0.0001 0.0002 0 0.0002 0.0001 0.0003 0.0002 0.0006 0.0001 0.0001 0.0001 0.0006 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0102 0.003 0.002 0.0011 0.001 0.0011 0.0106 0.0018 0.0016 0.0013 0.0015 0.0085 0.0148 0.0064 0.0036 0.0007 0.0006 0.0008
Endosome 0.0079 0.0071 0.0046 0.0019 0.0003 0.0026 0.004 0.0022 0.0041 0.0014 0.0007 0.0013 0.006 0.0017 0.0059 0.0007 0.0003 0.0074
Golgi 0.0013 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0013 0.0003 0.0002 0 0 0.0007
Lipid Particles 0.0032 0.0005 0.0003 0.0001 0.0002 0.0003 0.0056 0.0005 0.0006 0.0004 0.0006 0.0002 0.0032 0.0124 0.0006 0.0006 0.0003 0.0008
Mitochondria 0.0016 0.0004 0.0011 0.0003 0.0003 0.0003 0.0001 0.0001 0.0002 0.0002 0.0002 0.0005 0.0009 0.0008 0.0003 0.0001 0.0001 0.0001
None 0.8731 0.8675 0.9033 0.9054 0.9022 0.8727 0.8322 0.8523 0.8892 0.9347 0.8571 0.8327 0.8774 0.8836 0.902 0.9398 0.8957 0.8173
Nuclear Periphery 0.0085 0.0033 0.0016 0.0003 0.0004 0.0006 0.002 0.0005 0.0004 0.0004 0.0004 0.0004 0.0058 0.0019 0.0009 0.0004 0.0002 0.0015
Nucleolus 0.0006 0.0004 0.001 0.0001 0.0005 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0014 0.0002 0.0001 0.0001 0 0.0001
Nucleus 0.0024 0.0027 0.0027 0.0004 0.0009 0.0021 0.0008 0.0004 0.0003 0.0003 0.0008 0.0004 0.0047 0.0006 0.0006 0.0003 0.0001 0.0003
Peroxisomes 0.0027 0.0002 0.0002 0.0001 0.0004 0.0001 0.0001 0 0.0003 0.0001 0.0003 0.0003 0.0013 0.0008 0.0001 0.0001 0.0001 0.0001
Punctate Nuclear 0.0031 0.0012 0.0072 0.0003 0.0087 0.0071 0.0005 0.0004 0.0008 0.0004 0.0005 0.0006 0.004 0.0009 0.0006 0.0005 0.0004 0.0008
Vacuole 0.0019 0.0142 0.0034 0.0011 0.0002 0.0061 0.0021 0.0016 0.0013 0.0002 0.0004 0.0007 0.0014 0.0008 0.0014 0.0005 0.0002 0.0045
Vacuole Periphery 0.001 0.0007 0.0006 0.0001 0 0.0005 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0006 0.0003 0.0003 0.0001 0 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.1111 1.2361 1.3758 4.6895 3.1373 6.2188 4.3742 5.0611 4.6232 6.4323
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
103 1404 2651 1312 1113 1619 914 486 1216 3023 3565 1798

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 888.55 643.91 700.64 727.56 594.60 642.11 746.59 780.07 619.50 642.95 712.42 741.75
Standard Deviation 144.76 81.43 76.57 88.68 62.88 81.79 122.09 129.16 109.97 81.63 92.65 103.88
Intensity Change Log 2 — -0.464594 -0.342780 -0.288387 — 0.110901 0.328397 0.391684 — -0.205751 -0.035370 0.023618

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001242 0.000152 0.000100 0.000454 0.000307 0.000236 0.000569 0.001795 0.000386 0.000197 0.000220 0.000817
Bud Neck 0.003411 0.021148 0.007696 0.015794 0.030787 0.043891 0.008593 0.008821 0.028468 0.033328 0.007926 0.013910
Bud Site 0.014280 0.008679 0.011661 0.037964 0.004282 0.011512 0.024103 0.047338 0.005129 0.010196 0.014851 0.040498
Cell Periphery 0.000277 0.000103 0.000082 0.000229 0.000506 0.000201 0.000235 0.000448 0.000487 0.000156 0.000121 0.000289
Cytoplasm 0.235402* 0.476831* 0.557118* 0.461996* 0.379604* 0.334705* 0.396794* 0.434386* 0.367390* 0.400714* 0.516014* 0.454533*
Cytoplasmic Foci 0.092959 0.241229* 0.012673 0.018994 0.258828* 0.298624* 0.012982 0.043806 0.244778* 0.271968* 0.012753 0.025701
Eisosomes 0.000152 0.000108 0.000019 0.000052 0.000576 0.000250 0.000034 0.000076 0.000540 0.000184 0.000023 0.000058
Endoplasmic Reticulum 0.001838 0.000567 0.000547 0.002181 0.002327 0.000755 0.001647 0.001508 0.002286 0.000668 0.000829 0.001999
Endosome 0.035895 0.013354 0.001030 0.003244 0.024872 0.036710 0.002017 0.010487 0.025806 0.025863 0.001283 0.005202
Golgi 0.007312 0.003768 0.000071 0.000181 0.003122 0.007426 0.000223 0.015379 0.003477 0.005727 0.000110 0.004289
Lipid Particles 0.004620 0.006540 0.000480 0.001256 0.025431 0.018859 0.001124 0.005330 0.023668 0.013137 0.000645 0.002357
Mitochondria 0.009025 0.007667 0.000972 0.001149 0.009596 0.025002 0.001899 0.001436 0.009548 0.016951 0.001209 0.001227
Mitotic Spindle 0.001570 0.001685 0.006324 0.010650 0.000357 0.005102 0.027724 0.014820 0.000460 0.003515 0.011811 0.011777
None 0.000425 0.006789 0.003551 0.005029 0.003903 0.004595 0.006236 0.004201 0.003609 0.005614 0.004239 0.004805
Nuclear Periphery 0.000733 0.000476 0.001005 0.001430 0.000993 0.000543 0.001586 0.000909 0.000971 0.000512 0.001154 0.001289
Nuclear Periphery Foci 0.002594 0.000408 0.000168 0.000802 0.000390 0.000421 0.000547 0.000759 0.000576 0.000415 0.000265 0.000790
Nucleolus 0.000336 0.002007 0.000353 0.000619 0.002359 0.002717 0.000566 0.000460 0.002187 0.002387 0.000408 0.000576
Nucleus 0.160182* 0.152256* 0.369030* 0.339143* 0.165149* 0.109540 0.446953* 0.279018* 0.164728* 0.129379 0.389008* 0.322891*
Peroxisomes 0.006713 0.007995 0.000224 0.000867 0.013381 0.018648 0.000335 0.004937 0.012816 0.013700 0.000253 0.001967
Vacuole 0.414912* 0.046348 0.026295 0.097518 0.071752 0.076965 0.062174 0.123194 0.100819 0.062746 0.035493 0.104458
Vacuole Periphery 0.006123 0.001888 0.000600 0.000447 0.001478 0.003299 0.003661 0.000891 0.001871 0.002643 0.001385 0.000567

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.70 1.78 1.06 -4.38 -4.60 0.35 -0.98 -1.34 -1.65 -0.84 0.86 0.67 -0.95 -2.97 -2.58
Bud Neck -9.90 -3.55 -3.85 4.96 -1.52 -3.92 8.05 9.55 15.05 2.13 -1.85 8.86 7.79 13.04 -0.80
Bud Site 1.40 1.00 -1.33 -4.09 -3.48 -5.28 -5.13 -4.38 -2.76 -1.30 -4.58 -5.09 -6.76 -4.67 -3.68
Cell Periphery 3.14 3.83 1.33 -3.82 -5.36 3.84 3.34 1.86 -0.37 -0.11 4.54 5.09 3.71 -0.95 -2.09
Cytoplasm -9.89 -12.03 -5.59 10.10 16.36 4.41 3.36 0.60 -2.54 -2.02 -3.74 -12.28 -0.24 3.80 13.15
Cytoplasmic Foci -14.08 5.17 4.92 37.31 -2.33 -4.33 36.07 31.39 41.70 -6.61 -3.62 35.61 34.26 58.27 -6.17
Eisosomes 1.52 6.23 3.85 8.77 -17.68 4.85 8.09 7.58 10.52 -5.36 5.86 8.53 7.84 13.10 -16.87
Endoplasmic Reticulum 4.47 4.07 -3.02 -16.85 -17.12 2.30 -0.98 0.08 -8.18 3.14 2.65 1.60 -1.24 -18.21 -13.55
Endosome 1.71 3.93 3.66 10.54 -6.24 -4.35 11.94 8.78 13.91 -3.09 -0.54 12.98 11.64 18.63 -5.84
Golgi 0.09 1.16 1.15 8.08 -1.79 -5.25 6.38 -1.17 0.43 -2.31 -3.78 6.07 1.63 4.76 -2.35
Lipid Particles -3.05 5.46 3.91 10.54 -13.94 2.55 11.03 8.47 8.11 -1.11 4.92 11.53 10.71 13.69 -2.91
Mitochondria -0.58 0.91 0.92 6.86 0.22 -6.11 5.19 5.72 12.00 2.50 -4.14 5.57 5.77 13.63 1.13
Mitotic Spindle 0.04 -3.39 -3.23 -3.35 -0.35 -4.49 -6.18 -3.57 -1.96 2.24 -4.81 -8.00 -5.74 -3.07 1.25
None -7.32 -10.48 -5.69 3.02 -0.65 -0.60 -1.61 -0.70 0.00 0.96 -2.12 -0.43 -0.47 2.13 -0.14
Nuclear Periphery 2.83 0.06 -3.82 -9.09 -7.33 2.64 -8.15 -3.65 -9.76 6.10 3.05 -3.95 -6.38 -12.54 -4.74
Nuclear Periphery Foci 4.88 5.38 3.33 -8.38 -14.38 -0.23 -3.69 -4.54 -3.06 -0.32 1.33 2.09 -5.12 -6.67 -10.90
Nucleolus -7.66 -0.21 -1.58 7.18 -1.99 -0.28 2.42 2.87 3.98 1.60 -0.19 2.93 2.90 6.19 -0.29
Nucleus 0.50 -8.56 -7.50 -24.45 2.63 8.59 -27.61 -10.72 -15.63 11.09 5.71 -34.84 -22.60 -31.65 7.92
Peroxisomes -1.52 1.55 1.44 11.44 -1.30 -3.20 11.51 7.15 10.21 -1.80 -0.89 11.57 10.43 16.25 -2.19
Vacuole 13.38 13.72 8.57 -20.62 -22.55 -1.21 -4.46 -12.32 -11.96 -9.33 7.68 9.49 -12.27 -22.31 -23.91
Vacuole Periphery 0.55 1.04 1.12 3.71 0.75 -5.12 -0.45 4.88 8.95 1.35 -2.54 1.63 4.48 8.91 1.52
Standard name
Human Ortholog
Description High-affinity cysteine-specific transporter; has similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Yct1

Yct1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yct1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available