Standard name | |
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Human Ortholog | |
Description | Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 3 | 4 | 6 | 2 | 2 | 9 |
Bud | 1 | 2 | 0 | 1 | 2 | 8 | 11 | 10 | 22 | 7 | 13 | 18 | 1 | 0 | 0 | 0 | 0 | – | 5 | 0 | 5 | 2 | 6 | 8 |
Bud Neck | 1 | 5 | 2 | 0 | 1 | 4 | 3 | 5 | 3 | 4 | 2 | 7 | 6 | 2 | 2 | 0 | 2 | – | 1 | 0 | 0 | 2 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 3 | 3 | 1 | 0 | 3 | 1 | 2 | 1 | 2 | 2 | 3 | 5 | 6 | 7 | 12 | – | 1 | 2 | 3 | 6 | 2 | 3 |
Cytoplasm | 483 | 339 | 293 | 259 | 358 | 352 | 353 | 469 | 288 | 293 | 336 | 346 | 449 | 565 | 608 | 361 | 330 | – | 439 | 302 | 229 | 201 | 265 | 354 |
Endoplasmic Reticulum | 0 | 3 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 3 | 4 | 4 | 22 | 23 | – | 1 | 0 | 3 | 2 | 4 | 3 |
Endosome | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 4 | 0 | 1 | 2 | 1 | 0 | 2 | 5 | 2 | – | 2 | 2 | 3 | 1 | 5 | 3 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | – | 0 | 2 | 2 | 2 | 3 | 3 |
Mitochondria | 9 | 7 | 11 | 11 | 33 | 121 | 209 | 246 | 205 | 194 | 283 | 271 | 1 | 0 | 1 | 17 | 8 | – | 15 | 11 | 21 | 27 | 25 | 29 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 3 | 5 | 2 | 2 | 0 | 1 | 3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 4 | 0 | 3 | 2 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 1 | 1 | 1 | 1 | 2 | 5 | 6 | 8 | 9 | 9 | 14 | 17 | 0 | 0 | 1 | 0 | 0 | – | 9 | 0 | 6 | 1 | 3 | 6 |
Vac/Vac Membrane | 2 | 0 | 0 | 1 | 2 | 0 | 3 | 8 | 4 | 2 | 9 | 6 | 1 | 1 | 1 | 2 | 3 | – | 4 | 1 | 2 | 8 | 5 | 14 |
Unique Cell Count | 493 | 348 | 302 | 263 | 372 | 400 | 448 | 594 | 408 | 422 | 507 | 531 | 455 | 571 | 618 | 374 | 339 | 498 | 334 | 295 | 271 | 336 | 453 | |
Labelled Cell Count | 503 | 360 | 314 | 276 | 401 | 493 | 593 | 756 | 548 | 519 | 672 | 678 | 466 | 580 | 630 | 415 | 382 | 498 | 334 | 295 | 271 | 336 | 453 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 3.7 | 3.5 | 3.6 | 3.4 | 3.3 | 2.9 | 3.1 | 2.8 | 2.8 | 2.6 | 2.7 | 5.5 | 5.3 | 5.6 | 5.1 | 5.1 | 4.8 | 5.4 | 5.8 | 5.6 |
Std Deviation (1e-4) | 0.5 | 0.6 | 0.9 | 1.4 | 1.3 | 1.1 | 1.2 | 1.9 | 0.8 | 0.7 | 0.7 | 0.9 | 1.2 | 1.1 | 1.3 | 1.1 | 1.4 | 1.0 | 1.2 | 2.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.02 | -0.07 | -0.3 | -0.16 | -0.34 | -0.31 | -0.4 | -0.39 | 0.65 | 0.61 | 0.69 | 0.54 | 0.53 | 0.47 | 0.64 | 0.73 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9554 | 1.0819 | 1.1317 | 0.5978 | 1.0069 | 1.1556 | 1.9214 | 2.777 | 2.3032 | 1.6896 | 2.1144 | 2.6397 | 0.7644 | 1.5514 | 1.2101 | 1.1903 | 1.2512 | 1.3504 |
Actin | 0.0114 | 0.0006 | 0.0221 | 0.002 | 0.0177 | 0.0114 | 0.0079 | 0.0005 | 0.0022 | 0.0009 | 0.0006 | 0.0041 | 0.011 | 0.0008 | 0.0047 | 0.0003 | 0.0245 | 0.0042 |
Bud | 0.0024 | 0.0045 | 0.0032 | 0.0016 | 0.001 | 0.002 | 0.0021 | 0.0007 | 0.0034 | 0.0002 | 0.0038 | 0.0002 | 0.0003 | 0.001 | 0.001 | 0.0002 | 0.0006 | 0.0002 |
Bud Neck | 0.0018 | 0.0007 | 0.0093 | 0.0004 | 0.0012 | 0.0078 | 0.0006 | 0.0002 | 0.0008 | 0.0001 | 0.0003 | 0.0005 | 0.0031 | 0.0002 | 0.0011 | 0.0004 | 0.0014 | 0.0009 |
Bud Periphery | 0.0078 | 0.0027 | 0.0044 | 0.0021 | 0.0007 | 0.0029 | 0.0039 | 0.0005 | 0.0054 | 0.0004 | 0.0046 | 0.0003 | 0.0004 | 0.0008 | 0.002 | 0.0001 | 0.0006 | 0.0004 |
Bud Site | 0.0069 | 0.0154 | 0.0126 | 0.0003 | 0.0039 | 0.0026 | 0.0038 | 0.0052 | 0.0033 | 0.0001 | 0.0007 | 0.0001 | 0.0025 | 0.003 | 0.0071 | 0.0009 | 0.0008 | 0.0002 |
Cell Periphery | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.2355 | 0.2823 | 0.2383 | 0.2918 | 0.2112 | 0.2682 | 0.2361 | 0.3449 | 0.2134 | 0.0945 | 0.3731 | 0.2911 | 0.3178 | 0.5338 | 0.3752 | 0.5241 | 0.4583 | 0.4875 |
Cytoplasmic Foci | 0.0264 | 0.0085 | 0.0162 | 0.0088 | 0.023 | 0.0139 | 0.0116 | 0.0059 | 0.0154 | 0.0095 | 0.0055 | 0.0069 | 0.0117 | 0.0088 | 0.0067 | 0.0047 | 0.0321 | 0.01 |
Eisosomes | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0029 | 0.0012 | 0.0015 | 0.0014 | 0.0011 | 0.002 | 0.0026 | 0.0009 | 0.0025 | 0.0009 | 0.0006 | 0.0008 | 0.0024 | 0.0016 | 0.0015 | 0.0021 | 0.0021 | 0.0015 |
Endosome | 0.012 | 0.0016 | 0.0055 | 0.0024 | 0.0161 | 0.0044 | 0.006 | 0.0018 | 0.001 | 0.0032 | 0.0008 | 0.0016 | 0.0089 | 0.0019 | 0.0021 | 0.0029 | 0.012 | 0.0039 |
Golgi | 0.0022 | 0.0002 | 0.0022 | 0.0005 | 0.0085 | 0.002 | 0.0013 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0018 | 0.0005 | 0.0004 | 0.0001 | 0.0046 | 0.0006 |
Lipid Particles | 0.0043 | 0.0002 | 0.0022 | 0.0004 | 0.0307 | 0.0011 | 0.0011 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0025 | 0.0038 | 0.0004 | 0.0001 | 0.0032 | 0.0003 |
Mitochondria | 0.0032 | 0.0004 | 0.0015 | 0.0013 | 0.0045 | 0.0015 | 0.0008 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0008 | 0.0004 | 0.0007 | 0.0005 | 0.0019 | 0.0006 |
None | 0.6551 | 0.6694 | 0.6633 | 0.6304 | 0.6298 | 0.668 | 0.6983 | 0.6218 | 0.7399 | 0.8847 | 0.6048 | 0.6783 | 0.6159 | 0.4339 | 0.5874 | 0.4515 | 0.4259 | 0.4826 |
Nuclear Periphery | 0.0041 | 0.0009 | 0.0017 | 0.0038 | 0.0076 | 0.0022 | 0.0085 | 0.0017 | 0.0009 | 0.0006 | 0.0004 | 0.0007 | 0.0046 | 0.0006 | 0.0012 | 0.0013 | 0.0073 | 0.0016 |
Nucleolus | 0.0017 | 0.0008 | 0.0009 | 0.0016 | 0.0048 | 0.0004 | 0.0009 | 0.0005 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0008 | 0.0002 | 0.0005 | 0.0004 | 0.0005 | 0.0001 |
Nucleus | 0.0069 | 0.0048 | 0.0034 | 0.0126 | 0.0041 | 0.0022 | 0.0082 | 0.0026 | 0.0019 | 0.0008 | 0.0016 | 0.0016 | 0.0027 | 0.003 | 0.004 | 0.0046 | 0.0073 | 0.0025 |
Peroxisomes | 0.0051 | 0.0002 | 0.0039 | 0.0268 | 0.0113 | 0.0032 | 0.0006 | 0.0003 | 0.0009 | 0.0002 | 0.0003 | 0.0004 | 0.0021 | 0.0027 | 0.0004 | 0.0001 | 0.0088 | 0.0007 |
Punctate Nuclear | 0.007 | 0.0036 | 0.0061 | 0.0104 | 0.021 | 0.003 | 0.0037 | 0.0108 | 0.0066 | 0.0011 | 0.001 | 0.0123 | 0.0091 | 0.0019 | 0.0021 | 0.0046 | 0.0063 | 0.0016 |
Vacuole | 0.0023 | 0.0014 | 0.001 | 0.0008 | 0.0009 | 0.0008 | 0.0012 | 0.0007 | 0.0005 | 0.0013 | 0.0008 | 0.0004 | 0.0011 | 0.0009 | 0.0012 | 0.0008 | 0.001 | 0.0005 |
Vacuole Periphery | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0009 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.5039 | 11.2408 | 7.2985 | 11.4382 | 9.1706 | 20.4221 | 18.469 | 22.054 | 21.7727 | 15.2088 |
Translational Efficiency | 1.1649 | 1.0491 | 1.2666 | 0.7243 | 0.6856 | 1.357 | 1.1866 | 0.9417 | 0.8241 | 0.7655 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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