Standard name
Human Ortholog
Description Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.98 0.96 0.88 0.79 0.79 0.71 0.69 0.66 0.65 0.99 0.99 0.98 0.97 0.97 – 0.88 0.9 0.78 0.74 0.79 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.3 0.47 0.41 0.5 0.46 0.56 0.51 0 0 0 0.05 0 – 0 0 0.07 0.1 0.07 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – 3 4 6 2 2 9
Bud 1 2 0 1 2 8 11 10 22 7 13 18 1 0 0 0 0 – 5 0 5 2 6 8
Bud Neck 1 5 2 0 1 4 3 5 3 4 2 7 6 2 2 0 2 – 1 0 0 2 3 4
Bud Site 0 0 0 0 0 0 0 2 1 2 3 3 0 0 0 0 0 – – – – – – –
Cell Periphery 3 1 3 3 1 0 3 1 2 1 2 2 3 5 6 7 12 – 1 2 3 6 2 3
Cytoplasm 483 339 293 259 358 352 353 469 288 293 336 346 449 565 608 361 330 – 439 302 229 201 265 354
Endoplasmic Reticulum 0 3 2 0 0 1 2 0 0 0 2 0 3 4 4 22 23 – 1 0 3 2 4 3
Endosome 1 0 1 0 1 0 1 0 4 0 1 2 1 0 2 5 2 – 2 2 3 1 5 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 – 0 2 2 2 3 3
Mitochondria 9 7 11 11 33 121 209 246 205 194 283 271 1 0 1 17 8 – 15 11 21 27 25 29
Nucleus 0 0 0 0 0 1 1 4 3 5 2 2 0 1 3 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 1 0 1 0 4 0 3 2 0 2 0 0 0 – 0 0 0 0 0 0
Nucleolus 2 0 1 0 0 1 0 1 2 2 1 1 0 0 0 0 0 – 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 2 1 0 1 1 0 0 0 0 0 – 0 0 1 0 0 1
SpindlePole 1 1 1 1 2 5 6 8 9 9 14 17 0 0 1 0 0 – 9 0 6 1 3 6
Vac/Vac Membrane 2 0 0 1 2 0 3 8 4 2 9 6 1 1 1 2 3 – 4 1 2 8 5 14
Unique Cell Count 493 348 302 263 372 400 448 594 408 422 507 531 455 571 618 374 339 498 334 295 271 336 453
Labelled Cell Count 503 360 314 276 401 493 593 756 548 519 672 678 466 580 630 415 382 498 334 295 271 336 453


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.7 3.5 3.6 3.4 3.3 2.9 3.1 2.8 2.8 2.6 2.7 5.5 5.3 5.6 5.1 5.1 4.8 5.4 5.8 5.6
Std Deviation (1e-4) 0.5 0.6 0.9 1.4 1.3 1.1 1.2 1.9 0.8 0.7 0.7 0.9 1.2 1.1 1.3 1.1 1.4 1.0 1.2 2.2 1.5
Intensity Change (Log2) – – – 0.03 -0.02 -0.07 -0.3 -0.16 -0.34 -0.31 -0.4 -0.39 0.65 0.61 0.69 0.54 0.53 0.47 0.64 0.73 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9554 1.0819 1.1317 0.5978 1.0069 1.1556 1.9214 2.777 2.3032 1.6896 2.1144 2.6397 0.7644 1.5514 1.2101 1.1903 1.2512 1.3504
Actin 0.0114 0.0006 0.0221 0.002 0.0177 0.0114 0.0079 0.0005 0.0022 0.0009 0.0006 0.0041 0.011 0.0008 0.0047 0.0003 0.0245 0.0042
Bud 0.0024 0.0045 0.0032 0.0016 0.001 0.002 0.0021 0.0007 0.0034 0.0002 0.0038 0.0002 0.0003 0.001 0.001 0.0002 0.0006 0.0002
Bud Neck 0.0018 0.0007 0.0093 0.0004 0.0012 0.0078 0.0006 0.0002 0.0008 0.0001 0.0003 0.0005 0.0031 0.0002 0.0011 0.0004 0.0014 0.0009
Bud Periphery 0.0078 0.0027 0.0044 0.0021 0.0007 0.0029 0.0039 0.0005 0.0054 0.0004 0.0046 0.0003 0.0004 0.0008 0.002 0.0001 0.0006 0.0004
Bud Site 0.0069 0.0154 0.0126 0.0003 0.0039 0.0026 0.0038 0.0052 0.0033 0.0001 0.0007 0.0001 0.0025 0.003 0.0071 0.0009 0.0008 0.0002
Cell Periphery 0.0002 0.0003 0.0003 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0
Cytoplasm 0.2355 0.2823 0.2383 0.2918 0.2112 0.2682 0.2361 0.3449 0.2134 0.0945 0.3731 0.2911 0.3178 0.5338 0.3752 0.5241 0.4583 0.4875
Cytoplasmic Foci 0.0264 0.0085 0.0162 0.0088 0.023 0.0139 0.0116 0.0059 0.0154 0.0095 0.0055 0.0069 0.0117 0.0088 0.0067 0.0047 0.0321 0.01
Eisosomes 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0002 0 0.0001 0.0001 0 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0029 0.0012 0.0015 0.0014 0.0011 0.002 0.0026 0.0009 0.0025 0.0009 0.0006 0.0008 0.0024 0.0016 0.0015 0.0021 0.0021 0.0015
Endosome 0.012 0.0016 0.0055 0.0024 0.0161 0.0044 0.006 0.0018 0.001 0.0032 0.0008 0.0016 0.0089 0.0019 0.0021 0.0029 0.012 0.0039
Golgi 0.0022 0.0002 0.0022 0.0005 0.0085 0.002 0.0013 0.0001 0.0002 0.0001 0.0001 0.0001 0.0018 0.0005 0.0004 0.0001 0.0046 0.0006
Lipid Particles 0.0043 0.0002 0.0022 0.0004 0.0307 0.0011 0.0011 0.0002 0.0002 0.0004 0.0001 0.0002 0.0025 0.0038 0.0004 0.0001 0.0032 0.0003
Mitochondria 0.0032 0.0004 0.0015 0.0013 0.0045 0.0015 0.0008 0.0003 0.0005 0.0002 0.0004 0.0002 0.0008 0.0004 0.0007 0.0005 0.0019 0.0006
None 0.6551 0.6694 0.6633 0.6304 0.6298 0.668 0.6983 0.6218 0.7399 0.8847 0.6048 0.6783 0.6159 0.4339 0.5874 0.4515 0.4259 0.4826
Nuclear Periphery 0.0041 0.0009 0.0017 0.0038 0.0076 0.0022 0.0085 0.0017 0.0009 0.0006 0.0004 0.0007 0.0046 0.0006 0.0012 0.0013 0.0073 0.0016
Nucleolus 0.0017 0.0008 0.0009 0.0016 0.0048 0.0004 0.0009 0.0005 0.0005 0.0002 0.0003 0.0002 0.0008 0.0002 0.0005 0.0004 0.0005 0.0001
Nucleus 0.0069 0.0048 0.0034 0.0126 0.0041 0.0022 0.0082 0.0026 0.0019 0.0008 0.0016 0.0016 0.0027 0.003 0.004 0.0046 0.0073 0.0025
Peroxisomes 0.0051 0.0002 0.0039 0.0268 0.0113 0.0032 0.0006 0.0003 0.0009 0.0002 0.0003 0.0004 0.0021 0.0027 0.0004 0.0001 0.0088 0.0007
Punctate Nuclear 0.007 0.0036 0.0061 0.0104 0.021 0.003 0.0037 0.0108 0.0066 0.0011 0.001 0.0123 0.0091 0.0019 0.0021 0.0046 0.0063 0.0016
Vacuole 0.0023 0.0014 0.001 0.0008 0.0009 0.0008 0.0012 0.0007 0.0005 0.0013 0.0008 0.0004 0.0011 0.0009 0.0012 0.0008 0.001 0.0005
Vacuole Periphery 0.0005 0.0002 0.0002 0.0003 0.0009 0.0002 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0.0003 0.0001 0.0002 0.0003 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5039 11.2408 7.2985 11.4382 9.1706 20.4221 18.469 22.054 21.7727 15.2088
Translational Efficiency 1.1649 1.0491 1.2666 0.7243 0.6856 1.357 1.1866 0.9417 0.8241 0.7655

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1521 1570 320 1590 1567 1753 2096 1160 3088 3323 2416 2750

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 515.91 616.61 794.55 791.85 608.56 671.40 754.89 762.67 562.93 645.51 760.14 779.54
Standard Deviation 55.94 83.06 90.20 119.57 85.31 83.74 96.80 120.70 85.91 87.79 96.89 120.91
Intensity Change Log 2 — 0.257239 0.623019 0.618108 — 0.141773 0.310867 0.325659 — 0.195899 0.462502 0.467224

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000160 0.000278 0.000268 0.000896 0.000510 0.000302 0.000102 0.001584 0.000338 0.000291 0.000124 0.001186
Bud Neck 0.027698 0.031128 0.002150 0.015063 0.012741 0.057783 0.006606 0.007149 0.020108 0.045189 0.006016 0.011725
Bud Site 0.006082 0.010247 0.002514 0.076057 0.006084 0.025081 0.014906 0.030574 0.006083 0.018072 0.013264 0.056871
Cell Periphery 0.000555 0.000111 0.000164 0.000312 0.002493 0.000210 0.000111 0.000213 0.001538 0.000163 0.000118 0.000270
Cytoplasm 0.386397* 0.431759* 0.218895* 0.406252* 0.484646* 0.346862* 0.523434* 0.494151* 0.436253* 0.386973* 0.483097* 0.443330*
Cytoplasmic Foci 0.283195* 0.218665* 0.021415 0.016002 0.218788* 0.258049* 0.011278 0.035515 0.250512* 0.239442* 0.012621 0.024233
Eisosomes 0.000826 0.000115 0.000070 0.000046 0.000200 0.000203 0.000024 0.000063 0.000508 0.000162 0.000030 0.000053
Endoplasmic Reticulum 0.001811 0.000614 0.003497 0.004362 0.000943 0.000872 0.000653 0.001251 0.001371 0.000750 0.001030 0.003050
Endosome 0.007940 0.021601 0.003188 0.006437 0.010648 0.030850 0.001233 0.009993 0.009314 0.026480 0.001492 0.007937
Golgi 0.008044 0.002661 0.000100 0.006804 0.001484 0.009991 0.000152 0.004519 0.004715 0.006528 0.000145 0.005840
Lipid Particles 0.033709 0.010578 0.003118 0.001193 0.013896 0.012692 0.000567 0.001615 0.023655 0.011693 0.000905 0.001371
Mitochondria 0.042543 0.011492 0.000413 0.004592 0.003799 0.033528 0.001164 0.004254 0.022883 0.023117 0.001064 0.004449
Mitotic Spindle 0.000528 0.002191 0.000968 0.072949 0.000081 0.008021 0.017227 0.026444 0.000301 0.005266 0.015073 0.053332
None 0.011209 0.007363 0.006602 0.005854 0.007250 0.003465 0.006046 0.003972 0.009200 0.005307 0.006119 0.005060
Nuclear Periphery 0.000780 0.000696 0.001778 0.001738 0.000795 0.000548 0.001076 0.000927 0.000787 0.000618 0.001169 0.001396
Nuclear Periphery Foci 0.000278 0.000329 0.001103 0.000929 0.000373 0.000296 0.000210 0.000727 0.000326 0.000312 0.000329 0.000844
Nucleolus 0.002003 0.002746 0.001578 0.000829 0.002399 0.002130 0.000459 0.000487 0.002204 0.002421 0.000608 0.000685
Nucleus 0.155546* 0.156485* 0.685815* 0.288249* 0.187001* 0.107803 0.380361* 0.267430* 0.171508* 0.130803* 0.420818* 0.279467*
Peroxisomes 0.014204 0.012803 0.000396 0.002933 0.004740 0.015987 0.000177 0.001611 0.009401 0.014483 0.000206 0.002375
Vacuole 0.014685 0.075887 0.045740 0.084336 0.040095 0.081178 0.033716 0.101942 0.027579 0.078678 0.035309 0.091763
Vacuole Periphery 0.001807 0.002253 0.000228 0.004169 0.001036 0.004149 0.000498 0.005579 0.001416 0.003253 0.000462 0.004764

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.43 -3.85 -6.25 -4.32 -2.86 0.67 1.35 -0.71 -1.11 -1.44 0.26 1.26 -1.46 -1.82 -2.40
Bud Neck -1.24 12.55 8.90 9.89 -6.46 -15.59 5.42 5.51 18.02 0.40 -12.55 12.05 10.03 20.06 -3.07
Bud Site -2.90 2.24 -8.93 -7.73 -9.97 -7.49 -2.69 -5.20 0.97 -3.39 -7.99 -2.72 -10.27 -4.72 -7.86
Cell Periphery 11.73 9.57 9.29 -4.29 -0.37 2.26 2.38 2.30 1.96 -4.36 2.69 2.81 2.67 -0.99 -5.62
Cytoplasm -4.34 14.21 5.55 9.37 -10.28 14.87 2.26 5.72 -6.83 3.92 7.55 1.09 9.00 1.86 6.96
Cytoplasmic Foci 8.24 44.46 49.72 39.09 3.44 -5.09 41.20 35.92 40.85 -10.89 2.08 63.07 60.59 57.10 -6.75
Eisosomes 13.41 13.85 14.59 12.84 5.00 -0.10 6.38 4.73 11.03 -10.53 11.34 15.78 14.90 15.06 -11.13
Endoplasmic Reticulum 4.09 -6.39 -3.36 -6.19 2.28 0.29 -1.03 -4.54 -5.27 -5.74 3.29 -1.70 -4.73 -7.21 -4.19
Endosome -8.63 5.44 6.22 12.28 0.92 -11.92 10.98 3.50 14.13 -5.13 -14.73 14.03 6.77 19.51 -6.38
Golgi 8.64 13.48 5.57 0.34 -2.61 -8.57 8.23 -1.17 6.01 -2.92 -2.88 14.90 3.55 5.20 -3.79
Lipid Particles 12.18 17.48 19.02 10.81 6.12 0.92 12.98 11.98 14.03 -8.20 10.36 22.58 22.16 17.67 -5.11
Mitochondria 10.20 14.62 13.75 7.57 -4.77 -11.92 4.89 1.67 12.44 -2.65 0.01 14.69 13.37 14.29 -4.28
Mitotic Spindle -2.84 -0.80 -11.09 -10.48 -10.81 -5.24 -7.57 -6.66 -3.75 -1.52 -5.88 -7.48 -12.92 -10.30 -6.14
None 2.52 3.08 4.18 1.82 0.02 2.89 1.27 3.09 0.13 5.00 3.87 3.73 4.97 1.21 2.47
Nuclear Periphery 0.37 -6.84 -10.99 -19.71 -6.05 2.42 -8.28 -6.35 -10.18 1.93 1.48 -8.38 -13.51 -22.36 -6.33
Nuclear Periphery Foci -0.92 -6.95 -7.67 -9.34 3.33 0.91 1.99 -3.81 -3.79 -5.27 0.24 -2.28 -8.35 -7.88 -7.08
Nucleolus -2.19 1.39 4.64 7.58 3.24 0.52 3.61 3.67 8.50 0.50 -0.79 5.13 5.37 11.00 0.95
Nucleus -0.24 -31.50 -24.11 -23.48 17.96 13.78 -30.98 -12.40 -22.48 13.08 9.46 -42.09 -26.11 -33.32 15.70
Peroxisomes 0.78 15.38 13.85 11.28 -2.35 -9.53 7.38 5.57 14.61 -3.47 -5.80 16.76 14.21 18.30 -4.37
Vacuole -20.64 -8.42 -28.77 -16.28 -9.17 -11.67 -5.26 -19.57 -13.02 -16.82 -22.11 -12.49 -33.84 -20.78 -23.90
Vacuole Periphery -1.54 11.86 0.47 1.10 -2.39 -7.40 2.44 -2.18 1.17 -3.00 -7.24 6.01 -1.48 1.97 -3.66
Standard name
Human Ortholog
Description Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (18%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gtt2

Gtt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gtt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available