Standard name | |
---|---|
Human Ortholog | |
Description | Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 23 | 9 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 2 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 66 | 51 | 29 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 51 | 102 | 51 | 56 | 64 | 120 | 131 | 210 | 147 | 124 | 169 | 168 | 56 | 146 | 336 | 135 | 96 | 97 | 6 | 7 | 0 | 14 | 15 | 14 |
Endoplasmic Reticulum | 8 | 40 | 17 | 3 | 1 | 8 | 5 | 3 | 3 | 2 | 1 | 4 | 243 | 183 | 92 | 13 | 9 | 2 | 61 | 81 | 43 | 118 | 103 | 101 |
Endosome | 4 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 19 | 9 | 8 | 1 | 2 | 2 | 1 | 3 | 4 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 1 | 1 | 2 | 1 | 0 | 2 | 2 | 0 | 1 |
Mitochondria | 4 | 0 | 3 | 0 | 2 | 2 | 14 | 16 | 14 | 14 | 8 | 14 | 0 | 0 | 0 | 5 | 1 | 3 | 0 | 0 | 1 | 5 | 3 | 4 |
Nucleus | 1 | 0 | 1 | 0 | 0 | 2 | 10 | 14 | 7 | 4 | 2 | 0 | 1 | 0 | 1 | 31 | 34 | 27 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 24 | 24 | 16 | 3 | 36 | 62 | 101 | 82 | 56 | 59 | 50 | 30 | 5 | 34 | 21 | 213 | 176 | 176 | 61 | 84 | 55 | 91 | 93 | 67 |
Unique Cell Count | 90 | 150 | 81 | 61 | 88 | 174 | 205 | 281 | 182 | 163 | 206 | 190 | 331 | 366 | 451 | 337 | 261 | 272 | 137 | 183 | 110 | 241 | 230 | 205 |
Labelled Cell Count | 96 | 167 | 88 | 64 | 103 | 198 | 263 | 327 | 228 | 205 | 233 | 216 | 404 | 431 | 481 | 417 | 327 | 316 | 137 | 183 | 110 | 241 | 230 | 205 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.7 | 9.2 | 7.2 | 8.3 | 8.0 | 6.8 | 5.8 | 5.8 | 5.7 | 5.2 | 5.3 | 5.0 | 15.5 | 10.3 | 7.4 | 7.7 | 9.1 | 8.9 | 7.2 | 7.3 | 8.6 |
Std Deviation (1e-4) | 1.5 | 1.8 | 1.6 | 1.9 | 1.9 | 1.9 | 1.7 | 1.6 | 1.8 | 1.8 | 1.4 | 1.3 | 4.5 | 2.4 | 1.7 | 1.8 | 1.9 | 2.1 | 1.6 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.2 | 0.16 | -0.07 | -0.31 | -0.3 | -0.33 | -0.48 | -0.44 | -0.51 | 1.11 | 0.52 | 0.04 | 0.09 | 0.33 | 0.31 | 0.0 | 0.03 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 3.6 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.9 | 1.4 | 0.9 | 0.1 | 2.1 | 3.1 | 2.1 | 3.4 | 4.9 | -8.5 | -3.8 | 2.1 | -3.7 | -4.2 | -4.4 |
Endoplasmic Reticulum | -2.7 | -4.2 | -4.1 | -5.3 | 0 | -5.5 | -5.4 | -6.4 | -5.3 | 8.8 | 4.8 | -0.1 | -5.4 | -5.2 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 1.2 | 1.4 | 0 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 3.1 | 2.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.6 | 3.0 | 2.6 | 4.6 | 1.7 | 1.8 | 2.6 | 0.8 | -0.8 | 0 | -2.7 | -4.9 | 7.1 | 7.6 | 7.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.3828 | 9.7039 | 8.8004 | 8.5327 | 10.0092 | 8.9912 | 6.8142 | 8.4231 | 8.0806 | 8.2201 | 7.8838 | 8.0127 | 9.631 | 11.1724 | 11.2454 | 12.269 | 11.7887 | 11.1976 |
Actin | 0.0119 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.04 | 0.0001 | 0.0058 | 0.0004 | 0.0011 | 0.0095 | 0.0003 | 0.0001 | 0.0149 | 0.0002 | 0 | 0.002 |
Bud | 0.0001 | 0.0002 | 0.0002 | 0.0007 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0002 |
Bud Neck | 0.0008 | 0.0009 | 0.0007 | 0.0017 | 0.0014 | 0.0021 | 0.0006 | 0.0008 | 0.0026 | 0.0011 | 0.0013 | 0.0012 | 0.0012 | 0.0009 | 0.0011 | 0.0006 | 0.0003 | 0.0017 |
Bud Periphery | 0.0007 | 0.0008 | 0.0007 | 0.0028 | 0.0013 | 0.0019 | 0.0005 | 0.0004 | 0.0014 | 0.0004 | 0.0009 | 0.0007 | 0.0025 | 0.001 | 0.001 | 0.001 | 0.0002 | 0.0014 |
Bud Site | 0.0004 | 0.0007 | 0.0007 | 0.0007 | 0.0014 | 0.0003 | 0.0008 | 0.0008 | 0.0077 | 0.0002 | 0.0005 | 0.0002 | 0.0007 | 0.0009 | 0.0103 | 0.0003 | 0.0001 | 0.0003 |
Cell Periphery | 0.0049 | 0.0141 | 0.0092 | 0.0079 | 0.0072 | 0.0127 | 0.0022 | 0.0115 | 0.005 | 0.002 | 0.0062 | 0.0063 | 0.0206 | 0.0085 | 0.0064 | 0.0039 | 0.001 | 0.0147 |
Cytoplasm | 0.0184 | 0.0565 | 0.0333 | 0.0746 | 0.0989 | 0.0273 | 0.084 | 0.0973 | 0.1204 | 0.1601 | 0.0436 | 0.0386 | 0.0263 | 0.0494 | 0.0557 | 0.0458 | 0.0641 | 0.0185 |
Cytoplasmic Foci | 0.0049 | 0.002 | 0.0029 | 0.0009 | 0.0057 | 0.0012 | 0.0213 | 0.0075 | 0.0136 | 0.0087 | 0.0025 | 0.0024 | 0.0012 | 0.0051 | 0.0148 | 0.0047 | 0.0026 | 0.0054 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.51 | 0.4466 | 0.4959 | 0.5464 | 0.4348 | 0.4805 | 0.6498 | 0.5503 | 0.6158 | 0.5138 | 0.4326 | 0.5983 | 0.6626 | 0.4857 | 0.5227 | 0.5935 | 0.2229 | 0.4603 |
Endosome | 0.0604 | 0.0382 | 0.0527 | 0.0141 | 0.0736 | 0.0351 | 0.0517 | 0.0655 | 0.0615 | 0.1051 | 0.0445 | 0.0475 | 0.0279 | 0.0534 | 0.047 | 0.0913 | 0.0625 | 0.0445 |
Golgi | 0.004 | 0.0011 | 0.0013 | 0.0005 | 0.0017 | 0.0007 | 0.0071 | 0.0009 | 0.0161 | 0.0218 | 0.002 | 0.0028 | 0.0007 | 0.001 | 0.0109 | 0.0052 | 0.0019 | 0.0016 |
Lipid Particles | 0.0164 | 0.0095 | 0.009 | 0.0045 | 0.0031 | 0.003 | 0.0095 | 0.0151 | 0.0188 | 0.0102 | 0.011 | 0.0054 | 0.0099 | 0.0209 | 0.0195 | 0.0042 | 0.0996 | 0.0091 |
Mitochondria | 0.0017 | 0.0003 | 0.0008 | 0.0005 | 0.001 | 0.0008 | 0.0033 | 0.0002 | 0.0056 | 0.0013 | 0.0007 | 0.002 | 0.0013 | 0.0004 | 0.0026 | 0.0015 | 0.002 | 0.0009 |
None | 0.0074 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0029 | 0.0054 | 0.0021 | 0.0024 | 0.0005 | 0.0004 | 0.0005 | 0.0011 | 0.0022 | 0.0002 | 0.178 | 0.0006 |
Nuclear Periphery | 0.0786 | 0.0329 | 0.04 | 0.0397 | 0.0265 | 0.0494 | 0.0366 | 0.0191 | 0.0161 | 0.0218 | 0.0392 | 0.0294 | 0.0674 | 0.048 | 0.0359 | 0.0337 | 0.0433 | 0.0506 |
Nucleolus | 0.0006 | 0.0004 | 0.0006 | 0.0001 | 0.0078 | 0.0005 | 0.0002 | 0.0003 | 0.0009 | 0 | 0.0003 | 0.0001 | 0.0024 | 0.0004 | 0.0003 | 0.0001 | 0.0042 | 0.0004 |
Nucleus | 0.0077 | 0.0055 | 0.0054 | 0.0035 | 0.0334 | 0.0122 | 0.0055 | 0.0026 | 0.003 | 0.0015 | 0.0064 | 0.0036 | 0.0128 | 0.0038 | 0.0042 | 0.0022 | 0.003 | 0.0061 |
Peroxisomes | 0.0012 | 0 | 0 | 0 | 0.0001 | 0 | 0.0019 | 0.0001 | 0.0016 | 0.0001 | 0.0001 | 0.0014 | 0.0001 | 0.0001 | 0.0076 | 0.0001 | 0.0004 | 0.0008 |
Punctate Nuclear | 0.001 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0022 | 0.0005 | 0.002 | 0.0002 | 0.0003 | 0.0002 | 0.0012 | 0.0003 | 0.0014 | 0.0002 | 0.0001 | 0.0032 |
Vacuole | 0.2528 | 0.3786 | 0.3282 | 0.2845 | 0.2796 | 0.3468 | 0.0743 | 0.2158 | 0.0961 | 0.1363 | 0.3864 | 0.2343 | 0.1464 | 0.3014 | 0.2165 | 0.1998 | 0.2349 | 0.3509 |
Vacuole Periphery | 0.0161 | 0.0113 | 0.0177 | 0.0161 | 0.0211 | 0.0247 | 0.0048 | 0.0057 | 0.003 | 0.0124 | 0.0196 | 0.0153 | 0.0137 | 0.0173 | 0.0244 | 0.0115 | 0.0789 | 0.0269 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.0489 | 80.4639 | 87.5026 | 81.4939 | 32.1938 | 76.0012 | 129.575 | 136.5661 | 107.9797 | 98.1421 |
Translational Efficiency | 1.0739 | 1.1628 | 1.0509 | 0.8279 | 0.9264 | 0.9006 | 1.0465 | 0.8586 | 1.0685 | 0.8211 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2301 | 1163 | 1634 | 1507 | 1592 | 1978 | 318 | 93 | 3893 | 3141 | 1952 | 1600 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 928.05 | 970.23 | 1270.98 | 1668.92 | 1184.01 | 1002.29 | 1609.67 | 1773.76 | 1032.72 | 990.42 | 1326.16 | 1675.01 |
Standard Deviation | 156.69 | 170.55 | 192.04 | 352.35 | 210.03 | 161.48 | 198.25 | 348.52 | 219.97 | 165.62 | 230.04 | 352.98 |
Intensity Change Log 2 | — | 0.064124 | 0.453667 | 0.846640 | — | -0.240381 | 0.443084 | 0.583130 | — | -0.098611 | 0.447744 | 0.704881 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000118 | 0.000392 | 0.000329 | 0.001576 | 0.000112 | 0.000154 | 0.001324 | 0.002532 | 0.000115 | 0.000242 | 0.000491 | 0.001632 |
Bud Neck | 0.004771 | 0.001385 | 0.001191 | 0.001862 | 0.000671 | 0.002064 | 0.001427 | 0.010683 | 0.003094 | 0.001813 | 0.001230 | 0.002375 |
Bud Site | 0.006430 | 0.007645 | 0.003014 | 0.009898 | 0.001553 | 0.005251 | 0.004545 | 0.031926 | 0.004436 | 0.006138 | 0.003264 | 0.011178 |
Cell Periphery | 0.003793 | 0.002631 | 0.001157 | 0.021426 | 0.005793 | 0.000774 | 0.003395 | 0.023820 | 0.004611 | 0.001462 | 0.001522 | 0.021565 |
Cytoplasm | 0.085491 | 0.069519 | 0.067661 | 0.020209 | 0.011522 | 0.037790 | 0.007865 | 0.010523 | 0.055242 | 0.049539 | 0.057920 | 0.019646 |
Cytoplasmic Foci | 0.016636 | 0.029302 | 0.002793 | 0.001666 | 0.001995 | 0.011665 | 0.000429 | 0.020767 | 0.010649 | 0.018195 | 0.002408 | 0.002776 |
Eisosomes | 0.000106 | 0.000025 | 0.000040 | 0.000246 | 0.000020 | 0.000017 | 0.000143 | 0.000450 | 0.000071 | 0.000020 | 0.000056 | 0.000258 |
Endoplasmic Reticulum | 0.475077* | 0.455627* | 0.823915* | 0.870868* | 0.592740* | 0.400166* | 0.943519* | 0.676773* | 0.523194* | 0.420701* | 0.843400* | 0.859586* |
Endosome | 0.021153 | 0.035278 | 0.008971 | 0.008310 | 0.008000 | 0.019389 | 0.005369 | 0.031229 | 0.015774 | 0.025272 | 0.008384 | 0.009642 |
Golgi | 0.002093 | 0.005286 | 0.000697 | 0.004662 | 0.000722 | 0.001757 | 0.000570 | 0.023283 | 0.001532 | 0.003064 | 0.000676 | 0.005744 |
Lipid Particles | 0.008555 | 0.008120 | 0.005549 | 0.023090 | 0.001546 | 0.005016 | 0.010708 | 0.062616 | 0.005689 | 0.006165 | 0.006390 | 0.025387 |
Mitochondria | 0.001693 | 0.001237 | 0.000240 | 0.001562 | 0.000187 | 0.002016 | 0.000060 | 0.003607 | 0.001077 | 0.001728 | 0.000211 | 0.001681 |
Mitotic Spindle | 0.000032 | 0.000130 | 0.000219 | 0.000395 | 0.000009 | 0.000265 | 0.000260 | 0.006211 | 0.000023 | 0.000215 | 0.000226 | 0.000733 |
None | 0.001724 | 0.000938 | 0.000690 | 0.002372 | 0.000234 | 0.000929 | 0.000072 | 0.000043 | 0.001115 | 0.000932 | 0.000590 | 0.002237 |
Nuclear Periphery | 0.002335 | 0.002907 | 0.011813 | 0.000796 | 0.001775 | 0.011858 | 0.001370 | 0.001711 | 0.002106 | 0.008544 | 0.010112 | 0.000849 |
Nuclear Periphery Foci | 0.002200 | 0.013301 | 0.038949 | 0.005186 | 0.003241 | 0.004582 | 0.009741 | 0.032155 | 0.002626 | 0.007810 | 0.034191 | 0.006754 |
Nucleolus | 0.000627 | 0.000465 | 0.000018 | 0.000030 | 0.000113 | 0.000417 | 0.000002 | 0.000021 | 0.000417 | 0.000435 | 0.000015 | 0.000029 |
Nucleus | 0.009886 | 0.003543 | 0.005253 | 0.001340 | 0.000974 | 0.007044 | 0.000540 | 0.000693 | 0.006242 | 0.005748 | 0.004485 | 0.001302 |
Peroxisomes | 0.000498 | 0.000444 | 0.000044 | 0.000383 | 0.000077 | 0.000324 | 0.000057 | 0.000370 | 0.000326 | 0.000369 | 0.000046 | 0.000382 |
Vacuole | 0.345626* | 0.333442* | 0.027076 | 0.021489 | 0.362223* | 0.469080* | 0.008502 | 0.055360 | 0.352413* | 0.418858* | 0.024050 | 0.023458 |
Vacuole Periphery | 0.011157 | 0.028381 | 0.000381 | 0.002634 | 0.006493 | 0.019443 | 0.000103 | 0.005227 | 0.009249 | 0.022752 | 0.000336 | 0.002785 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.57 | -3.48 | -4.59 | -3.58 | -3.80 | -0.74 | -2.16 | -2.31 | -2.28 | -0.62 | -1.76 | -3.53 | -4.91 | -4.51 | -3.33 |
Bud Neck | 6.71 | 6.74 | 4.63 | -2.19 | -2.33 | -3.44 | -1.21 | -2.62 | -2.25 | -2.37 | 3.34 | 5.09 | 0.98 | -1.84 | -3.13 |
Bud Site | -0.92 | 3.01 | -2.93 | -1.98 | -4.75 | -4.19 | -1.42 | -3.02 | -2.68 | -2.65 | -2.15 | 0.90 | -5.04 | -3.88 | -5.26 |
Cell Periphery | 2.37 | 6.20 | -11.41 | -12.09 | -13.18 | 9.64 | 2.52 | -2.30 | -2.98 | -2.61 | 9.83 | 7.97 | -11.17 | -13.41 | -13.22 |
Cytoplasm | 3.04 | 3.13 | 17.33 | 10.26 | 12.06 | -10.13 | 0.99 | -0.35 | 3.72 | -0.71 | 1.91 | -1.50 | 12.44 | 9.80 | 11.01 |
Cytoplasmic Foci | -5.57 | 12.48 | 13.74 | 13.48 | 2.65 | -9.94 | 3.79 | -2.61 | -1.42 | -2.79 | -6.56 | 11.48 | 8.95 | 13.65 | -0.59 |
Eisosomes | 7.15 | 4.09 | -3.39 | -5.40 | -4.86 | 0.67 | -1.75 | -1.81 | -1.83 | -1.19 | 7.27 | 0.71 | -4.55 | -5.81 | -4.60 |
Endoplasmic Reticulum | 1.55 | -35.17* | -40.10* | -33.68* | -5.19 | 17.33 | -27.67 | -1.37 | -5.85 | 6.54 | 12.42 | -39.61 | -38.19 | -48.04* | -1.68 |
Endosome | -7.97 | 12.69 | 12.56 | 15.81 | 0.99 | -10.39 | 2.31 | -3.80 | -1.95 | -4.19 | -9.56 | 10.25 | 6.78 | 14.32 | -1.45 |
Golgi | -4.04 | 6.39 | -2.72 | 0.53 | -4.26 | -2.63 | 0.81 | -2.01 | -1.91 | -2.02 | -3.95 | 5.53 | -3.84 | -2.31 | -4.64 |
Lipid Particles | 0.35 | 2.53 | -6.99 | -6.88 | -8.00 | -6.35 | -2.59 | -3.51 | -3.31 | -2.90 | -0.80 | -1.00 | -9.06 | -8.79 | -8.05 |
Mitochondria | 1.55 | 6.60 | 0.26 | -0.95 | -4.28 | -5.20 | 2.55 | -1.11 | -0.48 | -1.15 | -2.47 | 6.50 | -1.71 | 0.08 | -4.34 |
Mitotic Spindle | -2.94 | -3.47 | -3.58 | -2.68 | -1.51 | -1.58 | -1.93 | -1.75 | -1.68 | -1.68 | -1.88 | -4.05 | -3.28 | -2.27 | -2.21 |
None | 2.26 | 2.98 | -0.88 | -2.15 | -2.53 | -1.72 | 1.00 | 1.19 | 2.37 | 0.77 | 0.67 | 2.15 | -1.77 | -2.03 | -2.66 |
Nuclear Periphery | -0.93 | -8.00 | 3.96 | 4.31 | 9.78 | -7.96 | 0.90 | 0.01 | 6.71 | -0.43 | -7.64 | -8.19 | 4.78 | 9.48 | 9.74 |
Nuclear Periphery Foci | -7.01 | -17.50 | -5.14 | 4.49 | 15.24 | -1.86 | -3.20 | -3.23 | -3.11 | -2.56 | -7.21 | -17.44 | -5.37 | 0.26 | 13.38 |
Nucleolus | 1.50 | 6.57 | 6.44 | 5.41 | -2.08 | -6.93 | 5.91 | 4.52 | 9.82 | -2.85 | -0.02 | 7.20 | 6.95 | 10.35 | -2.71 |
Nucleus | 7.24 | 4.89 | 13.59 | 2.82 | 6.52 | -8.81 | 0.21 | 0.33 | 8.26 | 0.05 | 0.80 | 1.99 | 11.66 | 7.92 | 6.23 |
Peroxisomes | 0.78 | 13.28 | 1.27 | 0.60 | -3.75 | -4.47 | 0.42 | -1.81 | -0.36 | -1.86 | -0.94 | 12.04 | -0.63 | -0.12 | -3.93 |
Vacuole | 1.06 | 45.67* | 46.41* | 32.64* | 1.77 | -9.86 | 40.62* | 16.84 | 23.23 | -3.31 | -8.54 | 60.49* | 59.17* | 62.75* | -0.08 |
Vacuole Periphery | -10.07 | 17.10 | 10.41 | 15.38 | -4.35 | -12.20 | 11.84 | 0.63 | 6.02 | -2.26 | -14.46 | 20.49 | 9.68 | 20.58 | -4.84 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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