Standard name | |
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Human Ortholog | |
Description | Sterol transporter that transfers sterols between membranes; may regulate and coordinate formation of contact sites between organelles; localizes to ER-mitochondrial contact sites in a Tom70p- and Tom71p-dependent manner; mitochondrial localization requires GRAM domain; also localizes to ER-vacuole contact sites, in a Vac8p-dependent manner; has GRAM and StART-like (VASt) domains; one of six StART-like domain-containing proteins in yeast; conserved across eukaryotes |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 7 | 14 | 8 | 12 | 4 | 20 | – | 0 | 3 | 4 | 7 | 5 | 0 | 5 | 5 | 12 | 12 | 6 | 9 | 21 | 15 | 33 | 46 |
Bud | 6 | 9 | 9 | 19 | 19 | 23 | 57 | – | 47 | 56 | 52 | 63 | 17 | 18 | 33 | 6 | 4 | 10 | 0 | 0 | 2 | 1 | 8 | 7 |
Bud Neck | 3 | 4 | 29 | 24 | 43 | 28 | 58 | – | 22 | 19 | 18 | 41 | 1 | 5 | 6 | 0 | 7 | 2 | 0 | 1 | 5 | 15 | 12 | 20 |
Bud Site | 0 | 2 | 0 | 2 | 4 | 3 | 20 | – | 15 | 15 | 15 | 17 | 5 | 1 | 5 | 2 | 2 | 3 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 1 | 2 | 0 | 3 |
Cytoplasm | 22 | 65 | 124 | 87 | 131 | 76 | 97 | – | 33 | 48 | 38 | 48 | 173 | 234 | 307 | 74 | 49 | 44 | 10 | 25 | 24 | 36 | 24 | 34 |
Endoplasmic Reticulum | 6 | 13 | 5 | 1 | 2 | 5 | 1 | – | 0 | 0 | 2 | 3 | 4 | 12 | 10 | 15 | 5 | 7 | 0 | 1 | 1 | 2 | 5 | 8 |
Endosome | 7 | 2 | 1 | 8 | 7 | 30 | 14 | – | 7 | 11 | 0 | 48 | 68 | 102 | 103 | 74 | 52 | 43 | 92 | 65 | 83 | 99 | 178 | 169 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 5 | 1 | 0 | 2 | 2 | 3 | 3 |
Mitochondria | 85 | 58 | 8 | 37 | 17 | 97 | 157 | – | 206 | 262 | 290 | 198 | 74 | 35 | 76 | 21 | 11 | 15 | 49 | 74 | 139 | 111 | 141 | 172 |
Nucleus | 2 | 1 | 4 | 2 | 0 | 4 | 10 | – | 6 | 10 | 10 | 5 | 1 | 0 | 2 | 2 | 4 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 1 | 3 | 1 | – | 0 | 1 | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 2 | 0 | 0 | 1 | 0 |
Nucleolus | 3 | 0 | 0 | 2 | 0 | 5 | 6 | – | 5 | 16 | 18 | 12 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 3 | 2 | 1 |
Peroxisomes | 2 | 0 | 0 | 6 | 4 | 10 | 4 | – | 16 | 17 | 6 | 31 | 23 | 65 | 93 | 35 | 9 | 9 | 19 | 8 | 18 | 39 | 72 | 75 |
SpindlePole | 35 | 19 | 51 | 69 | 149 | 135 | 332 | – | 131 | 212 | 143 | 274 | 60 | 76 | 111 | 110 | 87 | 75 | 20 | 21 | 34 | 38 | 74 | 90 |
Vac/Vac Membrane | 6 | 6 | 5 | 3 | 8 | 3 | 2 | – | 8 | 5 | 6 | 14 | 11 | 13 | 8 | 25 | 14 | 15 | 4 | 19 | 17 | 26 | 21 | 27 |
Unique Cell Count | 144 | 154 | 217 | 217 | 341 | 341 | 592 | 364 | 503 | 450 | 543 | 372 | 465 | 631 | 292 | 206 | 197 | 214 | 241 | 367 | 409 | 593 | 675 | |
Labelled Cell Count | 180 | 187 | 253 | 268 | 398 | 426 | 779 | 496 | 675 | 602 | 765 | 442 | 561 | 761 | 372 | 264 | 248 | 214 | 241 | 367 | 409 | 593 | 675 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 3.6 | 4.8 | 4.3 | 4.9 | 3.9 | 4.1 | – | 3.7 | 3.6 | 3.6 | 4.1 | 3.9 | 3.9 | 3.9 | 6.3 | 6.5 | 6.5 | 3.7 | 3.9 | 4.0 |
Std Deviation (1e-4) | 0.5 | 0.5 | 0.8 | 1.4 | 1.1 | 0.9 | 1.2 | – | 1.2 | 0.9 | 0.9 | 0.9 | 0.8 | 0.8 | 0.9 | 1.6 | 1.4 | 1.6 | 0.8 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.16 | 0.02 | -0.29 | -0.21 | – | -0.37 | -0.4 | -0.42 | -0.22 | -0.28 | -0.31 | -0.29 | 0.41 | 0.45 | 0.45 | -0.37 | -0.31 | -0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.0 | 2.2 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0046 | -0.3613 | -0.533 | -0.1271 | 0.4547 | -0.6388 | -0.4492 | -0.9054 | -0.7717 | -0.5936 | -0.094 | -0.8856 | -0.032 | -0.7634 | -0.8512 | 0.1585 | -1.3526 | -0.8285 |
Actin | 0.0158 | 0.0068 | 0.0135 | 0.0021 | 0.0639 | 0.0161 | 0.0374 | 0.0157 | 0.0137 | 0.0596 | 0.006 | 0.0279 | 0.0693 | 0.0067 | 0.0237 | 0.0613 | 0.0112 | 0.0162 |
Bud | 0.0006 | 0.0024 | 0.0009 | 0.0007 | 0.0058 | 0.0013 | 0.0013 | 0.0003 | 0.0005 | 0.0021 | 0.0032 | 0.0003 | 0.0006 | 0.0005 | 0.0012 | 0.0015 | 0.0002 | 0.0002 |
Bud Neck | 0.0065 | 0.0007 | 0.0081 | 0.0049 | 0.0024 | 0.0053 | 0.0037 | 0.0005 | 0.0116 | 0.0011 | 0.0007 | 0.0056 | 0.001 | 0.0014 | 0.0074 | 0.0033 | 0.0009 | 0.0071 |
Bud Periphery | 0.0009 | 0.0014 | 0.0013 | 0.0018 | 0.0081 | 0.0021 | 0.0019 | 0.0006 | 0.0006 | 0.0037 | 0.0048 | 0.0004 | 0.0007 | 0.0006 | 0.0022 | 0.0023 | 0.0002 | 0.0002 |
Bud Site | 0.008 | 0.0135 | 0.0055 | 0.0491 | 0.0365 | 0.0013 | 0.0251 | 0.0034 | 0.0046 | 0.0087 | 0.0161 | 0.0005 | 0.0022 | 0.013 | 0.0033 | 0.008 | 0.0003 | 0.0004 |
Cell Periphery | 0.0004 | 0.0009 | 0.0006 | 0.0001 | 0.0005 | 0.0003 | 0.0011 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0001 | 0.0001 |
Cytoplasm | 0.0208 | 0.0363 | 0.0185 | 0.0321 | 0.0058 | 0.0251 | 0.0376 | 0.018 | 0.0124 | 0.0216 | 0.0068 | 0.0308 | 0.017 | 0.0493 | 0.0137 | 0.0528 | 0.0345 | 0.0455 |
Cytoplasmic Foci | 0.1718 | 0.2449 | 0.2549 | 0.1405 | 0.0967 | 0.2314 | 0.2036 | 0.2909 | 0.2899 | 0.3318 | 0.3292 | 0.2496 | 0.1862 | 0.2537 | 0.2713 | 0.2928 | 0.2882 | 0.2694 |
Eisosomes | 0.0005 | 0.0009 | 0.0004 | 0.0001 | 0.0009 | 0.0005 | 0.0006 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0007 | 0.0009 | 0.0005 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.005 | 0.0042 | 0.0036 | 0.0048 | 0.0172 | 0.003 | 0.0075 | 0.0028 | 0.0048 | 0.0021 | 0.001 | 0.0025 | 0.0045 | 0.004 | 0.002 | 0.0018 | 0.0014 | 0.0068 |
Endosome | 0.0689 | 0.1118 | 0.09 | 0.1366 | 0.0333 | 0.0605 | 0.0848 | 0.1023 | 0.1224 | 0.0723 | 0.1591 | 0.1008 | 0.1409 | 0.0995 | 0.1166 | 0.1522 | 0.0582 | 0.1462 |
Golgi | 0.0222 | 0.0233 | 0.0181 | 0.0172 | 0.0172 | 0.0193 | 0.0272 | 0.0207 | 0.0382 | 0.0427 | 0.0942 | 0.0337 | 0.0391 | 0.0246 | 0.0385 | 0.0512 | 0.0349 | 0.0443 |
Lipid Particles | 0.2355 | 0.181 | 0.1901 | 0.1664 | 0.1805 | 0.2261 | 0.2511 | 0.1729 | 0.1637 | 0.1212 | 0.1004 | 0.2208 | 0.2604 | 0.1388 | 0.1774 | 0.1225 | 0.1824 | 0.2014 |
Mitochondria | 0.0103 | 0.0136 | 0.0202 | 0.0074 | 0.036 | 0.0149 | 0.0223 | 0.0053 | 0.0224 | 0.0174 | 0.0104 | 0.0068 | 0.0074 | 0.0083 | 0.0192 | 0.0151 | 0.0346 | 0.0105 |
None | 0.1832 | 0.1921 | 0.0818 | 0.0844 | 0.0655 | 0.1135 | 0.1163 | 0.214 | 0.0825 | 0.1641 | 0.0994 | 0.0647 | 0.0481 | 0.2453 | 0.0947 | 0.0212 | 0.0904 | 0.0526 |
Nuclear Periphery | 0.0208 | 0.0072 | 0.0112 | 0.0182 | 0.0462 | 0.0051 | 0.0128 | 0.0016 | 0.013 | 0.0012 | 0.0008 | 0.0024 | 0.0192 | 0.0019 | 0.0011 | 0.0007 | 0.0007 | 0.0029 |
Nucleolus | 0.0013 | 0.002 | 0.0046 | 0.0021 | 0.0288 | 0.0011 | 0.009 | 0.0013 | 0.001 | 0.0007 | 0.0013 | 0.0002 | 0.0014 | 0.0014 | 0.0009 | 0.0005 | 0.0001 | 0.0004 |
Nucleus | 0.0082 | 0.0054 | 0.0126 | 0.0583 | 0.0304 | 0.0018 | 0.0139 | 0.0026 | 0.0024 | 0.0004 | 0.0008 | 0.0009 | 0.0077 | 0.0027 | 0.0006 | 0.0008 | 0.0001 | 0.0009 |
Peroxisomes | 0.1937 | 0.1317 | 0.226 | 0.2497 | 0.3073 | 0.2516 | 0.1154 | 0.1332 | 0.1916 | 0.1392 | 0.1559 | 0.2414 | 0.1513 | 0.133 | 0.214 | 0.1919 | 0.2557 | 0.1761 |
Punctate Nuclear | 0.0141 | 0.0108 | 0.0295 | 0.0134 | 0.0122 | 0.0156 | 0.02 | 0.009 | 0.0156 | 0.0068 | 0.0048 | 0.0058 | 0.0374 | 0.0088 | 0.0067 | 0.0097 | 0.0035 | 0.0127 |
Vacuole | 0.0082 | 0.0075 | 0.0066 | 0.0066 | 0.0022 | 0.0027 | 0.0052 | 0.0031 | 0.0057 | 0.002 | 0.0035 | 0.0034 | 0.003 | 0.0046 | 0.0033 | 0.0078 | 0.0015 | 0.0047 |
Vacuole Periphery | 0.0036 | 0.0016 | 0.0019 | 0.0034 | 0.0025 | 0.0013 | 0.002 | 0.001 | 0.0029 | 0.0008 | 0.0013 | 0.0012 | 0.0018 | 0.0009 | 0.001 | 0.0017 | 0.0008 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.2146 | 5.6852 | 13.7106 | 14.1888 | 13.3991 | 10.5936 | 10.4187 | 14.3872 | 18.564 | 14.6797 |
Translational Efficiency | 0.8731 | 1.1888 | 0.6598 | 0.7254 | 0.5968 | 0.8091 | 0.8716 | 0.6258 | 0.6202 | 0.6302 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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