Standard name
Human Ortholog
Description S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated UPR; regulates HAC1 mRNA translocation, splicing and translation with KIN1 during ER stress; direct phosphorylation of Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the PM and sites of polarized growth; may regulate septin and cell wall organization; activation loop phosphorylation (T281) required for full kinase activity; orthologous to MARK/PAR-1, AMPK family members
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.1 0.05 0.05 0.07 0.12 0.09 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0.06 0.1 0.16 0.06 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.11 0 0 0 0 0 0 0 – 0 0 0 0.11 0.06 0.05 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.9 0.9 0.9 0.97 0.91 0.9 0.83 0.77 – 0.72 0.7 0.65 0.97 0.99 0.97 0.92 0.92 0.82 0.71 0.76 0.64 0.36 0.65 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.1 0.22 0.28 0.37 – 0.45 0.46 0.49 0 0 0 0.08 0 0 0 0 0.05 0.07 0.05 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 15 2 0 1 0 0 0 – 0 1 1 3 2 4 12 19 36 27 13 24 27 32 13
Bud 4 8 12 5 4 11 13 21 – 24 18 22 1 2 3 4 5 15 13 6 8 35 17 9
Bud Neck 0 0 1 0 0 0 0 1 – 0 0 0 3 2 0 4 8 0 45 16 36 37 21 12
Bud Site 0 1 1 0 0 1 0 1 – 2 1 1 0 0 0 2 2 3 – – – – – –
Cell Periphery 45 10 15 14 10 12 15 4 – 10 21 17 18 10 15 14 9 23 7 3 10 5 6 6
Cytoplasm 381 237 324 323 320 463 555 591 – 452 363 427 155 159 286 512 389 301 373 213 242 82 241 297
Endoplasmic Reticulum 0 7 0 1 3 2 6 0 – 2 3 4 5 2 2 30 17 22 20 2 13 7 8 12
Endosome 21 0 5 0 5 4 4 11 – 9 5 6 0 0 0 1 0 2 9 6 8 3 4 4
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 4 1 1 1 2 2
Mitochondria 11 8 12 20 36 116 190 279 – 287 237 320 0 0 0 43 19 10 9 6 17 16 18 10
Nucleus 1 1 0 1 0 0 2 4 – 5 2 4 0 0 0 2 5 1 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 4 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 2 0 1 1 0 0 3 1 – 2 2 0 0 0 0 0 0 0 1 0 0 0 1 0
Peroxisomes 4 1 9 2 4 0 2 1 – 0 0 0 0 0 0 0 0 5 0 0 0 0 0 0
SpindlePole 13 0 7 1 3 1 5 3 – 3 3 0 0 0 0 8 9 14 2 0 1 1 1 1
Vac/Vac Membrane 4 0 1 0 8 3 7 15 – 5 9 15 0 0 1 1 0 5 7 5 5 5 7 14
Unique Cell Count 422 264 359 334 352 517 668 763 631 517 653 159 161 294 554 425 367 529 280 379 228 370 392
Labelled Cell Count 489 288 390 368 394 613 802 936 801 665 818 185 177 311 633 482 437 529 280 379 228 370 392


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.9 4.8 5.0 4.6 4.0 4.1 4.1 – 3.7 3.7 3.8 6.6 6.6 7.0 4.9 5.1 6.9 6.9 6.9 7.0
Std Deviation (1e-4) 0.6 0.8 0.8 1.2 1.2 1.0 1.2 0.8 – 1.2 0.9 0.9 0.9 0.9 1.0 1.0 0.8 1.2 1.5 1.4 1.6
Intensity Change (Log2) – – – 0.06 -0.04 -0.24 -0.24 -0.24 – -0.37 -0.38 -0.32 0.46 0.46 0.54 0.04 0.09 0.52 0.53 0.53 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 1.9 3.4 5.6
Bud -1.6 0 0 0 0 – 0 0 0 0 0 -2.0 -2.9 -2.1 0.5
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 3.1 1.0 0.6 -1.4 -1.7 1.3
Cytoplasm 3.4 0.3 -0.3 -3.1 -5.2 – -6.8 -7.1 -8.6 2.9 3.5 3.6 1.2 0.6 -3.2
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 4.5 3.8 4.7
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 1.7 0 0 0 0 – 0 0 0 0 0 -3.2 2.7 0.8 -0.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes -2.0 0 0 0 0 – 0 0 0 0 0 0 0 0 -1.1
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 -0.6 0.2 1.5
Vacuole 0 0 0 0 2.2 – 0 0 2.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.031 0.0024 0.0294 0.0319 0.0726 0.0183 0.0582 0.0041 0.032 0.0489 0.023 0.0184 – – – – – –
Bud 0.0033 0.0065 0.0181 0.0122 0.0018 0.0032 0.0029 0.0025 0.0215 0.0105 0.0061 0.0008 – – – – – –
Bud Neck 0.009 0.0012 0.0052 0.0028 0.0246 0.1718 0.0092 0.0018 0.0009 0.0081 0.0014 0.22 – – – – – –
Bud Periphery 0.0078 0.0083 0.0535 0.0464 0.0038 0.0046 0.0076 0.0016 0.0538 0.0422 0.0267 0.0021 – – – – – –
Bud Site 0.0265 0.0538 0.029 0.0033 0.0014 0.004 0.0305 0.1016 0.0039 0.0147 0.0027 0.0027 – – – – – –
Cell Periphery 0.0063 0.0044 0.004 0.0033 0.0011 0.0023 0.0121 0.0028 0.0039 0.0058 0.0061 0.002 – – – – – –
Cytoplasm 0.5877 0.7837 0.6742 0.7756 0.6778 0.6564 0.611 0.8044 0.6671 0.6378 0.6784 0.6388 – – – – – –
Cytoplasmic Foci 0.0354 0.0115 0.0172 0.0129 0.0309 0.0157 0.0375 0.0127 0.0185 0.0203 0.0296 0.0056 – – – – – –
Eisosomes 0.0004 0.0001 0.0003 0.0002 0.0005 0.0002 0.0008 0.0001 0.0005 0.0009 0.0003 0.0001 – – – – – –
Endoplasmic Reticulum 0.0146 0.0044 0.0053 0.0075 0.0115 0.0056 0.0096 0.0043 0.0059 0.003 0.0045 0.0019 – – – – – –
Endosome 0.0298 0.006 0.0163 0.0246 0.0463 0.0101 0.018 0.0041 0.015 0.0226 0.0047 0.0065 – – – – – –
Golgi 0.0058 0.0013 0.0054 0.0053 0.0147 0.0025 0.0148 0.0015 0.0077 0.0095 0.0044 0.0032 – – – – – –
Lipid Particles 0.0052 0.0006 0.002 0.0014 0.0204 0.0012 0.0123 0.0017 0.007 0.0127 0.0122 0.001 – – – – – –
Mitochondria 0.0075 0.0004 0.0039 0.0033 0.0029 0.0011 0.0036 0.0001 0.0095 0.0034 0.0031 0.0118 – – – – – –
None 0.167 0.1074 0.1165 0.0544 0.0381 0.0874 0.1364 0.0514 0.1321 0.1431 0.1217 0.0808 – – – – – –
Nuclear Periphery 0.0225 0.0006 0.0072 0.0019 0.006 0.0065 0.0017 0.0005 0.002 0.0011 0.0019 0.0012 – – – – – –
Nucleolus 0.0011 0.0003 0.0003 0.0002 0.0009 0.0003 0.0004 0.0001 0.0002 0.0006 0.004 0.0001 – – – – – –
Nucleus 0.0142 0.0019 0.0023 0.0017 0.0241 0.0029 0.001 0.0008 0.001 0.0013 0.0025 0.0006 – – – – – –
Peroxisomes 0.0045 0.0002 0.0021 0.0021 0.0074 0.0007 0.0221 0.0002 0.0127 0.0056 0.054 0.0007 – – – – – –
Punctate Nuclear 0.0119 0.0006 0.0022 0.0012 0.0075 0.002 0.0061 0.0008 0.0017 0.0035 0.0103 0.0005 – – – – – –
Vacuole 0.0066 0.0039 0.0046 0.0067 0.0047 0.0025 0.0035 0.0029 0.0026 0.0038 0.0018 0.001 – – – – – –
Vacuole Periphery 0.0021 0.0002 0.001 0.0011 0.001 0.0005 0.0005 0.0002 0.0006 0.0006 0.0005 0.0003 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.5823 16.6114 15.9393 26.9272 22.8466 18.572 23.0942 24.2357 24.5365 27.6109
Translational Efficiency 0.8695 0.7716 0.8429 0.4577 0.5582 0.8693 0.6385 0.5414 0.5531 0.5602

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1039 1272 942 1339 1584 1792 2395 1679 2623 3064 3337 3018

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 833.94 823.07 852.79 977.99 623.02 757.93 916.88 929.05 706.57 784.97 898.79 950.76
Standard Deviation 96.02 104.75 95.95 124.80 70.84 106.97 107.57 119.80 131.62 110.80 108.33 124.44
Intensity Change Log 2 — -0.018928 0.032247 0.229876 — 0.282786 0.557454 0.576478 — 0.117876 0.280519 0.388374

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002405 0.001425 0.001676 0.016320 0.001464 0.001044 0.001882 0.003720 0.001837 0.001202 0.001824 0.009310
Bud Neck 0.031917 0.055042 0.013258 0.019725 0.095789 0.062666 0.035116 0.024881 0.070489 0.059501 0.028946 0.022594
Bud Site 0.079818 0.076939 0.079074 0.136295* 0.069525 0.064429 0.089136 0.123424* 0.073602 0.069622 0.086295 0.129134*
Cell Periphery 0.000378 0.000435 0.000364 0.000965 0.001966 0.000243 0.000234 0.000432 0.001337 0.000323 0.000271 0.000668
Cytoplasm 0.471354* 0.507385* 0.618748* 0.489726* 0.487583* 0.485127* 0.668674* 0.614056* 0.481154* 0.494367* 0.654581* 0.558895*
Cytoplasmic Foci 0.057510 0.192196* 0.083562 0.046174 0.196721* 0.192927* 0.037983 0.055883 0.141578 0.192624* 0.050849 0.051575
Eisosomes 0.000221 0.000574 0.000088 0.000533 0.002847 0.000404 0.000106 0.000263 0.001807 0.000475 0.000101 0.000383
Endoplasmic Reticulum 0.000486 0.000910 0.002040 0.001598 0.002548 0.000584 0.000331 0.000533 0.001731 0.000720 0.000814 0.001005
Endosome 0.010571 0.021928 0.007109 0.004839 0.011162 0.029401 0.002960 0.003401 0.010928 0.026298 0.004131 0.004039
Golgi 0.002162 0.009157 0.000794 0.002353 0.020404 0.008999 0.000426 0.001516 0.013178 0.009064 0.000530 0.001887
Lipid Particles 0.002619 0.003350 0.001687 0.001640 0.010334 0.004480 0.000457 0.000556 0.007278 0.004011 0.000804 0.001037
Mitochondria 0.001876 0.001970 0.000117 0.002427 0.024251 0.005927 0.000243 0.000909 0.015388 0.004284 0.000207 0.001582
Mitotic Spindle 0.008050 0.001297 0.001457 0.012540 0.000154 0.004113 0.003040 0.001695 0.003282 0.002944 0.002593 0.006506
None 0.000305 0.000652 0.001444 0.000305 0.004907 0.001033 0.000431 0.001014 0.003084 0.000875 0.000717 0.000699
Nuclear Periphery 0.000132 0.000058 0.000168 0.000116 0.000303 0.000115 0.000053 0.000043 0.000235 0.000092 0.000086 0.000076
Nuclear Periphery Foci 0.001226 0.000273 0.004702 0.001401 0.000379 0.000192 0.000653 0.000675 0.000714 0.000225 0.001796 0.000997
Nucleolus 0.000174 0.000265 0.000170 0.000127 0.000438 0.000379 0.000077 0.000059 0.000333 0.000332 0.000103 0.000089
Nucleus 0.025866 0.022279 0.066773 0.023707 0.029763 0.019797 0.028172 0.018465 0.028219 0.020827 0.039068 0.020791
Peroxisomes 0.003978 0.006323 0.003166 0.005690 0.006687 0.009203 0.003021 0.003411 0.005614 0.008007 0.003062 0.004422
Vacuole 0.298371* 0.095655 0.113346 0.232161* 0.029683 0.106748 0.126853 0.144837 0.136113 0.102143 0.123040 0.183580
Vacuole Periphery 0.000581 0.001887 0.000259 0.001358 0.003092 0.002190 0.000152 0.000226 0.002097 0.002064 0.000182 0.000729

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.69 0.38 -5.18 -6.47 -6.24 2.20 -0.97 -5.13 -7.17 -4.48 1.31 0.01 -6.39 -7.96 -7.15
Bud Neck -6.02 4.47 4.24 11.36 -0.68 6.16 13.96 15.93 11.35 3.26 2.48 13.41 16.13 15.96 3.75
Bud Site -1.14 -0.24 -3.71 -2.89 -3.28 1.36 -1.03 -5.74 -6.79 -4.79 0.13 -0.99 -6.75 -7.06 -5.75
Cell Periphery -2.05 -0.42 -6.09 -3.90 -5.67 14.47 14.80 12.91 -2.99 -3.91 13.12 14.37 10.04 -5.10 -7.73
Cytoplasm -4.26 -11.42 0.99 5.65 13.06 0.29 -20.06 -12.01 -11.76 6.32 -2.62 -23.28 -7.28 -4.86 15.10
Cytoplasmic Foci -25.25 -6.30 4.75 28.63 10.16 0.58 36.29 30.62 28.47 -7.16 -11.69 27.33 27.75 40.19 0.54
Eisosomes -5.89 7.44 -5.41 2.34 -8.75 13.80 15.81 14.70 3.33 -3.04 11.75 15.74 13.39 3.93 -7.41
Endoplasmic Reticulum -1.50 -8.82 -4.82 -1.75 2.68 6.15 7.01 6.02 0.15 -1.43 4.67 4.43 3.66 -1.52 -0.65
Endosome -8.86 3.57 8.27 13.88 4.63 -12.42 16.75 14.39 18.52 -0.86 -15.19 15.80 16.16 23.04 1.86
Golgi -7.65 1.70 0.63 9.49 -1.51 10.28 22.89 20.06 9.74 -2.08 5.10 20.54 17.54 13.48 -2.78
Lipid Particles -1.13 0.66 0.70 3.88 0.14 7.14 14.80 14.64 7.95 -1.61 5.08 11.89 11.39 8.69 -2.52
Mitochondria -0.43 1.51 0.16 1.35 -3.24 10.19 14.67 14.04 6.21 -1.33 9.40 13.95 12.82 6.24 -3.05
Mitotic Spindle 2.90 2.95 -0.91 -4.00 -4.08 -3.63 -3.21 -3.27 2.04 0.95 -0.11 0.69 -1.97 -1.89 -2.77
None -2.26 -3.75 -0.19 2.51 3.83 4.99 6.72 5.30 0.31 -1.16 4.70 5.80 5.38 0.78 0.27
Nuclear Periphery 4.20 -1.80 -0.72 -6.23 1.24 0.82 0.98 1.06 1.75 3.19 1.10 0.95 0.97 -1.03 0.25
Nuclear Periphery Foci 1.61 -5.24 -0.23 -8.84 8.23 0.86 -1.55 -1.79 -6.28 -0.56 1.82 -4.12 -1.20 -10.70 5.79
Nucleolus -2.05 0.03 1.34 4.40 2.06 0.59 6.09 6.41 5.20 3.55 -0.11 5.74 6.13 6.45 1.89
Nucleus 2.91 -12.21 -0.02 -3.79 13.25 5.97 -3.78 4.97 -1.29 9.08 6.38 -10.14 3.39 -3.65 14.24
Peroxisomes -4.62 0.93 -0.69 2.76 -1.47 -3.50 8.86 7.15 9.05 -1.51 -5.27 7.29 3.82 8.42 -2.18
Vacuole 26.10* 19.51 -0.71 -28.31 -21.11 -23.20 -32.92 -30.71 -13.90 -4.96 9.06 -0.97 -16.77 -29.30 -18.24
Vacuole Periphery -4.47 1.40 -0.86 3.18 -2.09 3.68 16.27 16.04 11.23 -1.29 0.07 14.16 9.40 8.78 -2.45
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated UPR; regulates HAC1 mRNA translocation, splicing and translation with KIN1 during ER stress; direct phosphorylation of Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the PM and sites of polarized growth; may regulate septin and cell wall organization; activation loop phosphorylation (T281) required for full kinase activity; orthologous to MARK/PAR-1, AMPK family members
Localization
Cell Percentages cytoplasm (64%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Kin2

Kin2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kin2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available