Standard name
Human Ortholog
Description Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII during osmotic shock; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; binds calmodulin; stimulates antisense transcription to activate CDC28; defines novel S-phase checkpoint with Mrc1p that prevent replication/transcription conflicts; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.09 0.05 0.18 0.24 0.23 0.27 0.22 0.21 0.33 0.23 0.31 0.25 0.2 0.25 0.21 0.1 0.12 0.13 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.95 0.94 0.94 0.9 0.93 0.88 0.87 0.87 0.88 0.99 0.99 0.92 0.96 0.97 0.96 0.91 0.95 0.95 0.94 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 2 2 1 0 0 0 0 0 0 0 0 3 0 0 1
Bud 1 3 4 1 3 4 25 12 11 16 18 13 1 2 0 3 3 0 0 1 1 8 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 9 16 147 130 148 212 176 183 220 178 182 149 110 120 92 43 47 52 0 1 3 0 0 2
Cytoplasm 98 348 787 510 600 730 722 797 584 662 505 529 533 469 403 429 375 398 96 325 769 551 508 519
Endoplasmic Reticulum 0 1 13 7 11 17 19 9 35 40 14 21 5 4 13 7 8 5 1 1 12 2 2 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 1 0 1 1 3 1 3
Golgi 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 2 1 0 0 2 3 0 0 2
Mitochondria 1 0 1 0 5 6 22 12 15 28 19 22 0 0 0 1 2 1 0 0 2 0 1 1
Nucleus 0 2 6 2 2 1 3 2 1 0 1 1 2 8 45 24 7 3 0 1 0 4 1 1
Nuclear Periphery 0 0 1 0 1 1 1 1 1 4 1 1 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0 2 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Vac/Vac Membrane 0 0 2 8 8 5 14 18 3 4 5 3 1 2 13 3 2 10 0 0 2 4 1 2
Unique Cell Count 99 350 804 535 641 780 798 858 661 762 580 602 538 476 438 446 385 415 106 341 808 586 526 552
Labelled Cell Count 109 370 962 658 778 977 983 1036 871 934 748 740 652 606 566 514 445 471 106 341 808 586 526 552


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.1 17.4 14.0 14.4 14.8 14.7 13.5 14.2 14.1 13.1 12.8 12.1 17.6 17.5 16.9 17.4 15.5 17.6 18.2 18.1 18.7
Std Deviation (1e-4) 2.5 3.5 2.2 2.2 2.4 2.3 2.4 2.5 2.5 2.4 2.5 2.6 3.4 3.7 3.7 4.4 4.2 4.2 3.3 3.5 3.4
Intensity Change (Log2) – – – 0.04 0.08 0.08 -0.04 0.03 0.01 -0.09 -0.13 -0.21 0.33 0.32 0.28 0.31 0.15 0.33 0.38 0.38 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 4.0 1.9 2.2 2.8 3.8 2.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.7 2.3 4.2 1.9 1.6 6.6 2.5 5.6 2.9 1.0 2.9 1.2 -4.1 -2.7 -2.6
Cytoplasm -2.6 -4.1 -4.2 -6.3 -4.8 -7.4 -8.3 -8.0 -7.6 1.7 0.8 -4.9 -1.8 -0.5 -2.0
Endoplasmic Reticulum -0.5 0.1 0.8 1.1 -1.0 3.9 4.0 1.1 2.3 -1.1 -1.2 1.6 -0.1 0.6 -0.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.4 2.9 3.9 5.2 4.8 5.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 1.6 8.1 5.1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.0 3.5 0 0 0 0 0 0 4.2 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 19.0239 21.1992 20.3625 18.864 17.9725 20.5494 20.9866 22.3812 21.8153 20.8444 20.7008 20.7923 19.4411 22.4106 21.4145 20.5784 20.7075 21.1955
Actin 0.0134 0.0026 0.0136 0.0303 0.026 0.0044 0.0005 0.0007 0.0047 0.0008 0.0027 0.0005 0.0071 0.0088 0.0065 0.0089 0.0082 0.0089
Bud 0.0004 0.0001 0.0002 0.0014 0.0009 0.0005 0.0003 0.0002 0.0025 0 0.0054 0.0008 0.0057 0.013 0.0168 0.0043 0.0056 0.0063
Bud Neck 0.0023 0.0004 0.0012 0.0063 0.0029 0.0039 0.0013 0.0008 0.0013 0.0014 0.0031 0.0044 0.001 0.0008 0.0008 0.0013 0.0043 0.007
Bud Periphery 0.0004 0 0.0002 0.0016 0.0013 0.0018 0.0002 0.0001 0.001 0 0.0085 0.0003 0.0028 0.0025 0.0069 0.0015 0.0023 0.0052
Bud Site 0.0021 0.0017 0.0043 0.0068 0.0009 0.0006 0.0023 0.0017 0.0024 0.0001 0.0032 0.0002 0.0079 0.0155 0.0131 0.0006 0.0013 0.0006
Cell Periphery 0.0014 0.0003 0.0006 0.0006 0.0008 0.0007 0.0005 0.0004 0.0003 0.0002 0.0018 0.0002 0.0012 0.001 0.0017 0.0003 0.0012 0.0008
Cytoplasm 0.9279 0.9899 0.9495 0.865 0.9029 0.972 0.9863 0.9772 0.9708 0.9929 0.9671 0.9824 0.933 0.9445 0.9241 0.9608 0.9646 0.9603
Cytoplasmic Foci 0.0134 0.001 0.0057 0.0133 0.0082 0.0018 0.0011 0.0005 0.0006 0.0004 0.0005 0.0007 0.0063 0.0012 0.0008 0.0022 0.0022 0.0009
Eisosomes 0.0001 0 0.0002 0.0004 0.001 0.0001 0 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0048 0.0012 0.0016 0.0029 0.0122 0.0024 0.0014 0.0015 0.0013 0.001 0.002 0.0018 0.0044 0.0013 0.0022 0.0017 0.0021 0.0013
Endosome 0.0072 0.0002 0.0014 0.0111 0.0115 0.0023 0.0003 0.0001 0 0.0001 0.0001 0 0.0015 0.0001 0.0001 0.0002 0.0001 0.0001
Golgi 0.0023 0.0001 0.0019 0.0039 0.0087 0.0005 0.0001 0 0 0 0.0001 0 0.0008 0.0001 0.0001 0.0001 0.0001 0.0001
Lipid Particles 0.0057 0.0001 0.0022 0.0068 0.0028 0.0005 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0036 0.0001 0.0001 0.0001 0.0013 0.0001
Mitochondria 0.0011 0 0.0007 0.0239 0.0068 0.0011 0.0002 0 0.0001 0 0 0 0.0051 0.0003 0.0006 0.0003 0.0001 0.0001
None 0.0052 0.0006 0.0022 0.0038 0.0017 0.0013 0.0017 0.0013 0.0011 0.0006 0.0006 0.0018 0.0031 0.0017 0.0016 0.0062 0.002 0.0013
Nuclear Periphery 0.0013 0.0003 0.0005 0.0032 0.0055 0.001 0.0004 0.0007 0.0012 0.0003 0.0005 0.0005 0.0038 0.0015 0.001 0.0026 0.0008 0.0011
Nucleolus 0.0003 0 0.0003 0.0015 0.0002 0.0002 0.0001 0 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0
Nucleus 0.0035 0.0012 0.0079 0.0045 0.002 0.0027 0.0023 0.0137 0.0106 0.0018 0.0031 0.0055 0.0052 0.0057 0.0215 0.0045 0.002 0.0044
Peroxisomes 0.0036 0 0.003 0.0057 0.0007 0.0001 0.0001 0 0 0 0 0 0.0016 0.0001 0.0001 0.0001 0 0
Punctate Nuclear 0.0023 0.0001 0.0026 0.0026 0.0009 0.0005 0.0003 0.0009 0.0015 0.0001 0.0003 0.0006 0.0039 0.0011 0.0006 0.0035 0.001 0.0012
Vacuole 0.001 0.0001 0.0003 0.0028 0.0013 0.0014 0.0005 0.0002 0.0002 0.0001 0.0006 0.0002 0.001 0.0004 0.0009 0.0004 0.0005 0.0003
Vacuole Periphery 0.0001 0 0 0.0013 0.0009 0.0003 0 0 0 0 0 0 0.0006 0 0.0001 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 71.2826 77.6734 55.3987 80.2977 80.9368 68.2923 83.6822 80.2811 76.2906 99.0266
Translational Efficiency 0.9619 0.8573 0.8621 0.5483 0.6772 1.1078 0.7869 0.7566 0.8361 0.6682

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII during osmotic shock; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; binds calmodulin; stimulates antisense transcription to activate CDC28; defines novel S-phase checkpoint with Mrc1p that prevent replication/transcription conflicts; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Hog1

Hog1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hog1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available