Standard name
Human Ortholog
Description Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.1 0.06 0.06 0.1 0.06 0.07 0 0.08 0 0 0.12 0.13 0.17 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.15 0.23 0.27 0.23 0.49 0.28 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.91 0.9 0.89 0.86 0.79 0.72 0.73 0.71 0.65 0.71 0.75 0.84 0.8 0.75 0.9 0.89 0.91 0.89 0.91 0.93 0.77 0.63 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0.06 0.08 0.18 0.1 0.18 0.26 0.24 0.35 0.23 0.34 0.32 0.06 0.09 0.09 0 0 0 0 0 0 0.14 0.18 0.16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.12 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0
Bud 0 0 0 0 0 0 0 2 0 1 1 2 0 0 0 1 0 0 0 0 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 6 3 10 5 10 1 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 11 6 4 7 16 11 13 6 12 5 0 25 41 39 32 18 11 1 2 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 13 7 4 0 0 0 0 1 0
Endosome 2 1 0 0 0 0 0 0 1 0 0 0 0 8 4 7 2 1 2 0 0 1 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1 0 1 0
Mitochondria 1 2 1 2 9 23 40 54 34 71 45 49 0 0 0 8 4 5 0 2 0 0 3 2
Nucleus 170 196 54 63 106 125 126 144 103 95 114 128 182 246 171 597 410 379 163 198 68 109 124 119
Nuclear Periphery 2 0 0 3 0 2 5 1 1 1 1 2 0 1 0 6 3 1 0 0 0 0 1 0
Nucleolus 15 13 5 13 12 28 45 47 51 34 55 55 12 27 21 17 9 16 5 3 0 20 35 29
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2 6 3 2 1 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 1 0 2 3 1 4 2 0 1 0 0 1 0 2
Vac/Vac Membrane 4 6 0 1 2 3 2 4 3 4 1 3 5 1 5 18 23 14 4 5 1 7 22 26
Unique Cell Count 183 215 60 71 123 158 174 198 145 146 161 171 216 309 228 666 459 416 183 219 74 143 196 191
Labelled Cell Count 201 229 66 86 138 198 230 272 202 229 229 250 229 333 245 705 481 432 183 219 74 143 196 191


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 9.5 7.9 7.1 7.1 6.3 5.5 5.5 6.1 4.6 5.3 5.1 10.1 9.0 8.9 9.5 10.9 10.0 8.0 8.6 9.3
Std Deviation (1e-4) 1.5 1.8 2.2 1.9 2.4 1.6 1.9 1.6 3.3 2.0 1.4 1.2 4.1 3.0 3.1 2.9 3.1 2.9 1.7 1.9 1.9
Intensity Change (Log2) – – – -0.15 -0.15 -0.34 -0.54 -0.53 -0.38 -0.78 -0.58 -0.63 0.35 0.19 0.17 0.27 0.47 0.34 0.02 0.12 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 -0.4 0 0 0.3 0.7 1.3 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.7 3.7 4.2 3.8 0 4.3 4.4 0 0 0 0 0 0
Nucleus -0.2 -0.7 -1.9 -2.8 -2.8 -2.9 -3.6 -3.0 -2.5 -1.1 -1.9 -2.5 -0.1 -0.2 0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.7 0.3 1.7 2.9 2.6 3.9 0 3.8 3.6 0 0.1 0.2 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.035 0 0.0022 0 0.049 0.0056 0.0157 0.0156 0.0288 0.0003 0.0221 0.0014 0.0017 0 0.0417 0 0.0004 0
Bud 0.0011 0 0.0001 0 0.0007 0.0003 0.0004 0 0.0003 0.0003 0.0007 0.0001 0.0003 0 0.0008 0.0001 0.0001 0.0001
Bud Neck 0.0017 0.0001 0.0002 0.0001 0.0023 0.0007 0.0011 0 0.0009 0.0009 0.0014 0.0007 0.0004 0 0.0006 0.0002 0.0003 0.0019
Bud Periphery 0.0018 0 0.0002 0 0.002 0.0004 0.0008 0 0.0009 0.0008 0.001 0.0001 0.0004 0 0.0009 0.0001 0.0002 0.0002
Bud Site 0.0079 0 0.0004 0 0.003 0.0072 0.0012 0.0001 0.0063 0.0005 0.0024 0.0002 0.0002 0 0.0039 0 0 0.0001
Cell Periphery 0.0005 0 0.0001 0 0.0006 0.0001 0.0001 0 0.0055 0.0001 0.0002 0 0.0001 0 0.0003 0 0 0
Cytoplasm 0.0089 0 0.004 0 0.0047 0.0002 0.0046 0 0.0021 0.0008 0.0164 0.0019 0.0119 0 0.0037 0.0001 0.0001 0
Cytoplasmic Foci 0.0192 0 0.0029 0 0.0081 0.003 0.0068 0 0.0035 0.0002 0.0169 0.0007 0.0137 0 0.0319 0 0 0
Eisosomes 0.001 0 0 0 0.0006 0.0001 0.0001 0.0001 0.0003 0 0.0002 0 0 0 0.0004 0 0 0
Endoplasmic Reticulum 0.003 0 0.002 0 0.0025 0.0002 0.0038 0 0.0024 0.0004 0.0016 0.0029 0.0039 0 0.0159 0 0.0001 0
Endosome 0.0127 0 0.0114 0 0.0146 0.0025 0.0123 0.0001 0.0085 0.0014 0.0223 0.0038 0.0059 0 0.1645 0 0.0001 0
Golgi 0.0071 0 0.002 0 0.0135 0.0011 0.0039 0.0004 0.0056 0.0001 0.0322 0.0007 0.0007 0 0.0841 0 0 0
Lipid Particles 0.0221 0 0.0054 0 0.0288 0.0045 0.0053 0 0.0099 0.0003 0.0054 0.0024 0.0062 0 0.0959 0 0 0
Mitochondria 0.0174 0.0001 0.0022 0.0001 0.0361 0.0008 0.0031 0.0003 0.0083 0.0012 0.0204 0.0017 0.0007 0.0001 0.0238 0.0002 0.0026 0.0003
None 0.0058 0 0.0006 0 0.0024 0.0003 0.0016 0 0.0005 0.0004 0.0379 0.0005 0.0052 0 0.0018 0 0.0001 0
Nuclear Periphery 0.0092 0.0013 0.0077 0.0002 0.0079 0.0049 0.0216 0.0004 0.0028 0.0068 0.0332 0.0022 0.3297 0.0002 0.0113 0.001 0.2171 0.0002
Nucleolus 0.0193 0.0145 0.0134 0.0336 0.0785 0.0434 0.0222 0.0062 0.005 0.0197 0.0549 0.0335 0.0381 0.0262 0.029 0.0483 0.0803 0.034
Nucleus 0.7979 0.982 0.9333 0.9655 0.7218 0.9177 0.8804 0.9764 0.8985 0.9592 0.714 0.945 0.1742 0.9731 0.4675 0.9475 0.6932 0.9629
Peroxisomes 0.0172 0 0.0052 0 0.0089 0.0032 0.0039 0.0001 0.0057 0.0003 0.0062 0.0004 0.0004 0 0.0069 0 0 0
Punctate Nuclear 0.006 0.002 0.0022 0.0004 0.0063 0.0034 0.0066 0.0002 0.0007 0.0044 0.005 0.0011 0.3983 0.0003 0.0084 0.0024 0.0008 0.0001
Vacuole 0.0031 0 0.0018 0 0.0051 0.0002 0.0026 0 0.003 0.0014 0.0019 0.0003 0.0024 0 0.004 0 0.0003 0.0001
Vacuole Periphery 0.002 0 0.0026 0 0.0027 0.0002 0.0016 0 0.0008 0.0005 0.0037 0.0002 0.0057 0 0.0029 0 0.0041 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.8913 20.6787 17.9019 20.7129 23.4936 12.3303 16.2039 21.4027 16.3284 18.8163
Translational Efficiency 0.6027 0.6125 0.6365 0.5799 0.5525 0.8385 0.854 0.5009 0.7705 0.6896

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
515 713 205 949 1239 1538 141 2652 1754 2251 346 3601

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 739.88 839.70 1204.64 1198.64 878.67 947.49 1170.06 1232.24 837.92 913.35 1190.55 1223.39
Standard Deviation 92.73 187.55 177.06 195.60 121.69 143.01 216.62 224.35 130.31 166.22 194.90 217.65
Intensity Change Log 2 — 0.182583 0.703239 0.696035 — 0.108789 0.413189 0.487890 — 0.142991 0.553043 0.586777

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000490 0.000832 0.001052 0.000783 0.000330 0.000518 0.000884 0.000592 0.000377 0.000617 0.000983 0.000642
Bud Neck 0.004078 0.007132 0.011046 0.024032 0.003956 0.010265 0.005819 0.030333 0.003992 0.009272 0.008916 0.028672
Bud Site 0.002557 0.001621 0.001048 0.002557 0.000456 0.001413 0.000588 0.003569 0.001073 0.001479 0.000861 0.003303
Cell Periphery 0.000170 0.000260 0.000034 0.000092 0.000054 0.000057 0.000033 0.000186 0.000088 0.000121 0.000034 0.000161
Cytoplasm 0.001494 0.002991 0.000279 0.002401 0.000399 0.000343 0.000179 0.003278 0.000720 0.001182 0.000239 0.003047
Cytoplasmic Foci 0.000597 0.000678 0.000030 0.000617 0.000055 0.000370 0.000028 0.000238 0.000214 0.000468 0.000029 0.000338
Eisosomes 0.000024 0.000038 0.000067 0.000034 0.000033 0.000048 0.000049 0.000034 0.000030 0.000044 0.000060 0.000034
Endoplasmic Reticulum 0.001874 0.003569 0.001930 0.001737 0.002030 0.003161 0.002095 0.002146 0.001984 0.003290 0.001997 0.002038
Endosome 0.001097 0.000439 0.000047 0.000989 0.000168 0.000547 0.000035 0.000512 0.000441 0.000513 0.000042 0.000638
Golgi 0.000387 0.000454 0.000074 0.001833 0.000047 0.000363 0.000064 0.000261 0.000147 0.000392 0.000070 0.000675
Lipid Particles 0.000410 0.000112 0.000038 0.000258 0.000023 0.000452 0.000033 0.000054 0.000137 0.000344 0.000036 0.000108
Mitochondria 0.001889 0.001598 0.001358 0.003304 0.000397 0.001459 0.001016 0.002286 0.000835 0.001503 0.001219 0.002555
Mitotic Spindle 0.000869 0.002546 0.000394 0.011156 0.000056 0.001684 0.000044 0.006483 0.000294 0.001957 0.000251 0.007715
None 0.005135 0.013364 0.001126 0.002659 0.002769 0.001571 0.000838 0.003991 0.003464 0.005307 0.001009 0.003640
Nuclear Periphery 0.000193 0.000119 0.000088 0.001146 0.000041 0.000277 0.000069 0.001216 0.000086 0.000227 0.000080 0.001197
Nuclear Periphery Foci 0.000377 0.000819 0.000034 0.000947 0.000131 0.000545 0.000084 0.000446 0.000204 0.000631 0.000055 0.000578
Nucleolus 0.058934 0.039488 0.021087 0.009122 0.042273 0.027538 0.014338 0.005947 0.047165 0.031323 0.018336 0.006784
Nucleus 0.917239* 0.922189* 0.958212* 0.928876* 0.946146* 0.947054* 0.972301* 0.935751* 0.937658* 0.939178* 0.963953* 0.933939*
Peroxisomes 0.001126 0.000969 0.001357 0.000273 0.000399 0.000519 0.000872 0.000107 0.000612 0.000662 0.001159 0.000151
Vacuole 0.000507 0.000540 0.000590 0.003595 0.000136 0.001127 0.000548 0.001756 0.000245 0.000941 0.000573 0.002241
Vacuole Periphery 0.000554 0.000243 0.000108 0.003590 0.000102 0.000690 0.000082 0.000814 0.000235 0.000549 0.000097 0.001545

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.07 -3.85 -2.65 -1.01 2.66 -2.22 -8.11 -10.54 -1.75 3.31 -3.17 -9.58 -6.77 -1.80 5.48
Bud Neck -2.08 -5.39 -9.90 -8.68 -5.82 -7.76 -3.46 -21.50 -15.46 -18.88 -7.30 -7.18 -23.42 -17.86 -16.03
Bud Site 0.75 1.08 -0.09 -1.76 -2.91 -2.27 -0.77 -5.55 -3.33 -5.55 -0.91 0.07 -4.16 -3.72 -5.44
Cell Periphery -0.91 1.59 1.00 3.13 -4.69 -0.17 1.07 -3.41 -4.05 -5.23 -1.02 1.85 -1.42 -0.48 -5.87
Cytoplasm -1.46 1.31 -0.36 1.41 -2.80 0.38 1.25 -8.01 -9.39 -10.45 -1.31 1.57 -5.50 -4.19 -9.73
Cytoplasmic Foci -0.64 1.82 0.65 1.20 -2.05 -1.65 0.81 -1.21 1.03 -1.98 -1.56 1.96 -0.01 1.64 -2.78
Eisosomes -3.91 -8.99 -4.27 0.31 7.31 -5.66 -4.09 0.14 6.66 4.34 -6.78 -9.35 -1.96 6.15 8.87
Endoplasmic Reticulum -4.57 -0.53 -0.16 6.04 0.61 -4.70 -0.39 -1.33 3.93 -0.53 -6.48 -0.53 -1.25 6.14 -0.55
Endosome 0.93 1.62 0.74 -0.51 -3.11 -1.45 1.06 -1.22 0.63 -4.08 -0.27 1.86 -0.04 0.38 -4.81
Golgi -0.41 1.55 -1.13 -0.89 -1.68 -1.77 -2.11 -2.29 0.73 -1.96 -1.71 0.99 -1.75 -0.16 -2.18
Lipid Particles 0.89 1.27 0.81 -0.12 -1.23 -1.21 -1.13 -2.54 1.10 -1.96 -0.85 1.13 0.52 1.09 -1.78
Mitochondria 0.29 0.49 -1.33 -1.90 -3.06 -4.04 -8.24 -8.26 -2.48 -5.16 -1.91 -2.00 -5.39 -3.02 -5.30
Mitotic Spindle -1.42 -0.80 -3.17 -1.83 -1.18 -2.09 0.17 -6.41 -4.07 -6.42 -2.53 -0.92 -7.02 -4.29 -2.51
None -3.51 3.98 2.48 4.97 -3.11 1.44 2.55 -1.30 -4.58 -8.93 -1.87 3.87 -0.10 2.21 -8.57
Nuclear Periphery 0.80 0.53 -3.07 -3.57 -3.46 -3.16 -2.55 -5.91 -4.34 -5.69 -2.50 -0.99 -6.60 -5.46 -6.45
Nuclear Periphery Foci -1.15 0.80 -0.62 0.88 -1.16 -1.31 0.58 -1.35 0.63 -2.34 -1.73 0.51 -1.41 1.12 -1.78
Nucleolus 4.01 7.48 12.47 10.28 4.46 6.13 8.63 17.49 12.31 2.82 7.09 10.25 21.37 16.10 5.66
Nucleus -0.24 -3.99 -2.11 -2.01 2.21 -0.24 -6.51 4.75 4.56 10.40 -0.16 -4.90 2.14 2.24 6.73
Peroxisomes 0.13 -0.43 1.72 3.60 7.49 -1.18 -2.65 13.07 3.86 3.97 -0.45 -3.09 3.08 5.37 8.61
Vacuole -0.30 -0.95 -3.23 -3.22 -3.06 -2.26 -4.62 -4.92 -1.35 -3.52 -2.31 -4.96 -5.85 -2.67 -4.61
Vacuole Periphery 1.45 2.03 -1.96 -2.45 -2.61 -2.54 0.93 -2.82 0.24 -2.91 -1.85 2.00 -3.12 -1.58 -3.75
Standard name
Human Ortholog
Description Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Msl5

Msl5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msl5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available