Standard name | |
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Human Ortholog | |
Description | Aspartic protease; hyperglycosylated member of the yapsin family of proteases, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; involved in nutrient limitation-induced cleavage of the extracellular inhibitory domain of signaling mucin Msb2p, resulting in activation of the filamentous growth MAPK pathway; involved with other yapsins in the cell wall integrity response; role in KEX2-independent processing of the alpha factor precursor |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 4 | 5 | 11 | 15 | 20 | – | 4 | 6 | 6 | 7 | 0 | 0 | 0 | 0 | 3 | 3 | 10 | 11 | 9 | 1 | 2 | 5 |
Bud | 1 | 2 | 0 | 5 | 4 | 5 | 7 | – | 9 | 9 | 5 | 4 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 3 | 2 | 1 | 4 | 5 |
Bud Neck | 0 | 0 | 10 | 1 | 0 | 0 | 0 | – | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 6 | 6 | 10 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 4 | 4 | 26 | 8 | 13 | 22 | 22 | – | 11 | 10 | 9 | 13 | 2 | 15 | 10 | 10 | 12 | 19 | 7 | 3 | 8 | 0 | 2 | 3 |
Cytoplasm | 285 | 206 | 249 | 274 | 303 | 320 | 372 | – | 239 | 263 | 210 | 244 | 18 | 102 | 152 | 75 | 83 | 94 | 146 | 116 | 127 | 15 | 6 | 20 |
Endoplasmic Reticulum | 26 | 35 | 9 | 20 | 32 | 70 | 127 | – | 12 | 29 | 42 | 41 | 8 | 48 | 11 | 3 | 4 | 6 | 128 | 107 | 69 | 16 | 54 | 63 |
Endosome | 0 | 3 | 1 | 0 | 2 | 2 | 5 | – | 0 | 3 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 3 | 1 | 0 | 0 |
Golgi | 1 | 2 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 9 | 3 | 0 | 2 | 3 | 27 | 27 | – | 34 | 30 | 79 | 37 | 0 | 0 | 0 | 1 | 0 | 1 | 37 | 18 | 53 | 4 | 1 | 4 |
Nucleus | 1 | 1 | 0 | 3 | 3 | 4 | 6 | – | 3 | 3 | 3 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 0 | 0 | 3 | 0 | 1 | – | 2 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 5 | 9 | 5 | 6 | 34 | 35 | 27 | – | 14 | 21 | 6 | 13 | 0 | 0 | 8 | 3 | 2 | 4 | 18 | 12 | 17 | 2 | 2 | 9 |
Unique Cell Count | 327 | 252 | 271 | 304 | 373 | 451 | 553 | 301 | 342 | 332 | 334 | 25 | 149 | 169 | 84 | 92 | 115 | 375 | 292 | 310 | 50 | 78 | 118 | |
Labelled Cell Count | 337 | 268 | 304 | 324 | 408 | 500 | 616 | 328 | 378 | 363 | 369 | 30 | 169 | 182 | 93 | 107 | 134 | 375 | 292 | 310 | 50 | 78 | 118 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.7 | 6.1 | 6.1 | 7.1 | 6.0 | 6.1 | – | 5.4 | 5.4 | 4.6 | 5.1 | 8.0 | 7.6 | 7.0 | 8.3 | 8.4 | 8.1 | – | 10.0 | 10.7 |
Std Deviation (1e-4) | 0.8 | 1.0 | 0.9 | 1.6 | 1.8 | 1.2 | 1.1 | – | 1.4 | 1.0 | 0.8 | 1.0 | 1.9 | 1.4 | 1.6 | 2.0 | 2.6 | 2.4 | – | 3.0 | 2.7 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.23 | -0.0 | 0.01 | – | -0.18 | -0.18 | -0.39 | -0.25 | 0.39 | 0.33 | 0.2 | 0.45 | 0.47 | 0.42 | – | 0.72 | 0.82 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 1.2 | 1.5 | 1.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -2.9 | 0 | 0 | 0 | – | 0 | -2.4 | 0 | -2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -3.5 | -3.2 | -2.5 | -3.2 | – | -2.9 | -3.5 | 0 | -2.8 | 0 | 0.2 | -1.4 | 0.6 | 0.9 | 1.9 |
Cytoplasm | -0.7 | -3.8 | -6.7 | -7.7 | – | -4.2 | -5.0 | -8.2 | -5.9 | -3.2 | -6.2 | -0.7 | -0.7 | -0.5 | -2.9 |
Endoplasmic Reticulum | 1.8 | 2.7 | 5.1 | 7.1 | – | 0.4 | 2.6 | 0 | 4.0 | 0 | 8.3 | 1.6 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.1 | 3.7 | – | 5.7 | 5.0 | 0 | 5.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 3.8 | 3.4 | 2.1 | – | 1.9 | 2.6 | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2053 | 2.1984 | 1.8673 | 1.413 | 1.3432 | 1.7874 | 1.328 | 1.9047 | 2.177 | 1.6283 | 1.2501 | 1.6439 | 1.2258 | 2.0871 | 2.4209 | 1.4743 | 1.5451 | 1.7103 |
Actin | 0.0075 | 0.0064 | 0.0028 | 0.0033 | 0.0005 | 0.0022 | 0.1005 | 0.0009 | 0.0333 | 0.0901 | 0.0005 | 0.0132 | 0.0094 | 0.0016 | 0.026 | 0.0253 | 0.0501 | 0.0254 |
Bud | 0.0004 | 0.0009 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0013 | 0.0012 | 0.0003 | 0.0006 | 0.0001 | 0.0017 | 0.0007 | 0.001 | 0.0042 | 0.0019 | 0.0007 | 0.002 |
Bud Neck | 0.0049 | 0.0007 | 0.0011 | 0.0019 | 0.0007 | 0.0013 | 0.0026 | 0.0003 | 0.0022 | 0.0102 | 0.0005 | 0.0117 | 0.0018 | 0.001 | 0.0018 | 0.0014 | 0.0016 | 0.0023 |
Bud Periphery | 0.0006 | 0.0012 | 0.0005 | 0.0009 | 0.0002 | 0.0003 | 0.0031 | 0.0059 | 0.0007 | 0.0013 | 0.0003 | 0.004 | 0.0015 | 0.0021 | 0.004 | 0.0025 | 0.0012 | 0.0043 |
Bud Site | 0.007 | 0.0165 | 0.0038 | 0.001 | 0.0008 | 0.0002 | 0.0148 | 0.0053 | 0.0013 | 0.0067 | 0.0002 | 0.002 | 0.0033 | 0.0235 | 0.0152 | 0.0047 | 0.0044 | 0.0011 |
Cell Periphery | 0.0009 | 0.0033 | 0.0014 | 0.001 | 0.0002 | 0.0005 | 0.0023 | 0.0044 | 0.0023 | 0.0033 | 0.0008 | 0.0024 | 0.0017 | 0.0033 | 0.0014 | 0.0017 | 0.0004 | 0.0006 |
Cytoplasm | 0.3173 | 0.4665 | 0.4388 | 0.3597 | 0.4913 | 0.5277 | 0.3099 | 0.4339 | 0.4223 | 0.3983 | 0.4687 | 0.4849 | 0.3931 | 0.5311 | 0.4015 | 0.4921 | 0.5397 | 0.5221 |
Cytoplasmic Foci | 0.0391 | 0.0251 | 0.0296 | 0.0139 | 0.017 | 0.0192 | 0.0364 | 0.0098 | 0.0101 | 0.0126 | 0.0093 | 0.0132 | 0.021 | 0.0073 | 0.0243 | 0.0154 | 0.025 | 0.017 |
Eisosomes | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0009 | 0.0002 | 0.0004 | 0.0017 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.2248 | 0.2176 | 0.2271 | 0.2125 | 0.2265 | 0.1691 | 0.1372 | 0.2052 | 0.2294 | 0.1442 | 0.1439 | 0.1009 | 0.2475 | 0.2216 | 0.2102 | 0.1929 | 0.1342 | 0.2119 |
Endosome | 0.046 | 0.0238 | 0.0474 | 0.0612 | 0.0671 | 0.0267 | 0.015 | 0.015 | 0.0218 | 0.0252 | 0.0567 | 0.0076 | 0.02 | 0.0066 | 0.0357 | 0.0346 | 0.0656 | 0.014 |
Golgi | 0.009 | 0.0046 | 0.0099 | 0.0235 | 0.0114 | 0.0031 | 0.0061 | 0.003 | 0.0071 | 0.0085 | 0.016 | 0.0019 | 0.0034 | 0.0013 | 0.0087 | 0.0172 | 0.0179 | 0.0051 |
Lipid Particles | 0.0327 | 0.0101 | 0.0203 | 0.0295 | 0.0247 | 0.0182 | 0.0083 | 0.005 | 0.0069 | 0.008 | 0.0225 | 0.0033 | 0.0144 | 0.0067 | 0.0122 | 0.0353 | 0.0301 | 0.0084 |
Mitochondria | 0.0075 | 0.0072 | 0.0132 | 0.0244 | 0.0017 | 0.0024 | 0.0021 | 0.0008 | 0.0018 | 0.014 | 0.0025 | 0.0015 | 0.0031 | 0.0042 | 0.0098 | 0.0179 | 0.0062 | 0.0211 |
None | 0.2497 | 0.1907 | 0.1715 | 0.2199 | 0.1183 | 0.201 | 0.3197 | 0.2887 | 0.2433 | 0.2449 | 0.2604 | 0.3346 | 0.2274 | 0.1649 | 0.2174 | 0.1294 | 0.083 | 0.1295 |
Nuclear Periphery | 0.02 | 0.007 | 0.0086 | 0.0192 | 0.0147 | 0.0096 | 0.0064 | 0.0072 | 0.0059 | 0.0074 | 0.0052 | 0.0034 | 0.0234 | 0.0057 | 0.0065 | 0.0086 | 0.0056 | 0.007 |
Nucleolus | 0.0013 | 0.0005 | 0.0003 | 0.0005 | 0.0036 | 0.0018 | 0.0007 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0009 | 0.001 | 0.0003 | 0.0007 | 0.0009 | 0.0005 | 0.0003 |
Nucleus | 0.0038 | 0.0043 | 0.0033 | 0.008 | 0.0055 | 0.0031 | 0.0032 | 0.0028 | 0.0035 | 0.0031 | 0.0026 | 0.003 | 0.0089 | 0.0048 | 0.0032 | 0.0033 | 0.0024 | 0.0048 |
Peroxisomes | 0.0053 | 0.0019 | 0.0103 | 0.0026 | 0.0023 | 0.0017 | 0.0164 | 0.0002 | 0.001 | 0.0077 | 0.0004 | 0.0012 | 0.004 | 0.0034 | 0.0036 | 0.0032 | 0.0221 | 0.0076 |
Punctate Nuclear | 0.0114 | 0.0022 | 0.002 | 0.0016 | 0.003 | 0.0014 | 0.0089 | 0.0006 | 0.0012 | 0.0036 | 0.0005 | 0.0031 | 0.0057 | 0.0017 | 0.0037 | 0.0015 | 0.0044 | 0.0084 |
Vacuole | 0.0078 | 0.0088 | 0.0065 | 0.0121 | 0.0087 | 0.0077 | 0.0038 | 0.0085 | 0.0042 | 0.0069 | 0.007 | 0.0042 | 0.007 | 0.0075 | 0.0076 | 0.0084 | 0.0036 | 0.0059 |
Vacuole Periphery | 0.0025 | 0.0005 | 0.0012 | 0.0026 | 0.0016 | 0.0027 | 0.0005 | 0.0009 | 0.0006 | 0.0011 | 0.0016 | 0.0006 | 0.0016 | 0.0003 | 0.0018 | 0.0011 | 0.001 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 94.2088 | 41.5316 | 35.0986 | 46.725 | 42.6674 | 84.483 | 58.1772 | 54.9129 | 63.5953 | 67.7191 |
Translational Efficiency | 0.9298 | 0.9531 | 0.8734 | 1.0221 | 1.1566 | 1.5182 | 0.9964 | 1.0143 | 0.9653 | 0.9982 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
80 | 1266 | 564 | 768 | 1498 | 2088 | 656 | 2059 | 1578 | 3354 | 1220 | 2827 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 721.46 | 819.10 | 1211.28 | 1021.31 | 732.39 | 761.38 | 1244.40 | 1114.75 | 731.84 | 783.17 | 1229.09 | 1089.37 |
Standard Deviation | 131.53 | 120.92 | 193.01 | 271.84 | 102.73 | 108.66 | 228.58 | 210.31 | 104.41 | 116.84 | 213.51 | 232.41 |
Intensity Change Log 2 | — | 0.183120 | 0.747541 | 0.501430 | — | 0.056005 | 0.764766 | 0.606036 | — | 0.120484 | 0.756244 | 0.555074 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000279 | 0.000735 | 0.002628 | 0.006575 | 0.000239 | 0.000940 | 0.000987 | 0.006187 | 0.000241 | 0.000863 | 0.001746 | 0.006292 |
Bud Neck | 0.018089 | 0.011336 | 0.005440 | 0.012616 | 0.008874 | 0.020962 | 0.004596 | 0.011775 | 0.009341 | 0.017328 | 0.004986 | 0.012004 |
Bud Site | 0.007834 | 0.010868 | 0.009248 | 0.068325 | 0.004311 | 0.014759 | 0.014414 | 0.038997 | 0.004489 | 0.013291 | 0.012026 | 0.046965 |
Cell Periphery | 0.001488 | 0.000574 | 0.001148 | 0.003912 | 0.000631 | 0.000518 | 0.001268 | 0.004760 | 0.000675 | 0.000539 | 0.001213 | 0.004529 |
Cytoplasm | 0.446384* | 0.383041* | 0.154201* | 0.275902* | 0.465165* | 0.361498* | 0.209151* | 0.353188* | 0.464213* | 0.369630* | 0.183747* | 0.332192* |
Cytoplasmic Foci | 0.194578* | 0.227821* | 0.020257 | 0.070764 | 0.186017* | 0.207157* | 0.026056 | 0.042772 | 0.186451* | 0.214957* | 0.023375 | 0.050376 |
Eisosomes | 0.000521 | 0.000460 | 0.000144 | 0.000535 | 0.000617 | 0.000697 | 0.000101 | 0.000895 | 0.000612 | 0.000607 | 0.000121 | 0.000797 |
Endoplasmic Reticulum | 0.042659 | 0.075320 | 0.489413* | 0.136799* | 0.068977 | 0.062943 | 0.475308* | 0.211631* | 0.067643 | 0.067615 | 0.481828* | 0.191302* |
Endosome | 0.028382 | 0.027599 | 0.018046 | 0.030087 | 0.012345 | 0.031930 | 0.016048 | 0.015774 | 0.013158 | 0.030295 | 0.016972 | 0.019662 |
Golgi | 0.003923 | 0.007497 | 0.001229 | 0.010223 | 0.008184 | 0.014174 | 0.001389 | 0.006332 | 0.007968 | 0.011654 | 0.001315 | 0.007389 |
Lipid Particles | 0.046611 | 0.037640 | 0.019582 | 0.054955 | 0.041640 | 0.045877 | 0.017987 | 0.047194 | 0.041892 | 0.042768 | 0.018724 | 0.049302 |
Mitochondria | 0.007641 | 0.007044 | 0.000531 | 0.004964 | 0.006151 | 0.011948 | 0.000545 | 0.002198 | 0.006227 | 0.010097 | 0.000539 | 0.002949 |
Mitotic Spindle | 0.000119 | 0.004612 | 0.002102 | 0.022985 | 0.002959 | 0.007002 | 0.003063 | 0.010514 | 0.002815 | 0.006100 | 0.002619 | 0.013902 |
None | 0.002541 | 0.002417 | 0.001218 | 0.008883 | 0.005089 | 0.002921 | 0.002646 | 0.001887 | 0.004960 | 0.002730 | 0.001986 | 0.003788 |
Nuclear Periphery | 0.002118 | 0.002776 | 0.022779 | 0.001757 | 0.002579 | 0.002046 | 0.019617 | 0.001645 | 0.002556 | 0.002321 | 0.021079 | 0.001675 |
Nuclear Periphery Foci | 0.010763 | 0.006049 | 0.102294 | 0.033141 | 0.003026 | 0.002801 | 0.068520 | 0.021057 | 0.003418 | 0.004027 | 0.084134 | 0.024339 |
Nucleolus | 0.000817 | 0.000869 | 0.000155 | 0.000894 | 0.000848 | 0.001158 | 0.000224 | 0.000338 | 0.000846 | 0.001049 | 0.000192 | 0.000489 |
Nucleus | 0.102204 | 0.074936 | 0.036354 | 0.024641 | 0.127505 | 0.081663 | 0.040032 | 0.052678 | 0.126222 | 0.079124 | 0.038332 | 0.045061 |
Peroxisomes | 0.003315 | 0.005567 | 0.000256 | 0.002311 | 0.003598 | 0.007896 | 0.000364 | 0.001463 | 0.003584 | 0.007017 | 0.000314 | 0.001693 |
Vacuole | 0.078657 | 0.110176 | 0.112520 | 0.224735* | 0.049598 | 0.117001 | 0.097096 | 0.166181 | 0.051071 | 0.114424 | 0.104227 | 0.182088 |
Vacuole Periphery | 0.001076 | 0.002664 | 0.000456 | 0.004996 | 0.001647 | 0.004111 | 0.000589 | 0.002536 | 0.001618 | 0.003565 | 0.000527 | 0.003204 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.97 | -2.62 | -6.78 | -6.22 | -2.66 | -6.20 | -4.28 | -10.90 | -9.39 | -8.78 | -7.06 | -3.63 | -12.87 | -11.31 | -6.70 |
Bud Neck | 1.08 | 1.95 | 0.85 | -0.93 | -5.23 | -7.62 | 3.39 | -2.73 | 6.64 | -8.30 | -6.03 | 3.54 | -2.59 | 5.09 | -9.57 |
Bud Site | -0.64 | -0.52 | -8.28 | -9.93 | -10.04 | -7.70 | -3.51 | -15.39 | -9.62 | -6.41 | -7.82 | -4.52 | -18.55 | -13.96 | -12.04 |
Cell Periphery | 1.45 | 0.43 | -2.44 | -5.72 | -4.41 | 1.97 | -2.94 | -9.51 | -9.88 | -6.56 | 1.95 | -4.12 | -10.79 | -11.34 | -8.22 |
Cytoplasm | 2.01 | 9.04 | 5.32 | 8.51 | -8.44 | 12.45 | 20.75 | 12.83 | 0.94 | -11.64 | 12.56 | 29.52 | 16.37 | 5.38 | -16.35 |
Cytoplasmic Foci | -1.73 | 8.76 | 6.28 | 24.27 | -10.76 | -3.56 | 31.17 | 30.86 | 37.22 | -4.96 | -5.37 | 35.58 | 30.21 | 45.56 | -10.72 |
Eisosomes | 0.58 | 4.20 | 0.61 | 0.01 | -5.48 | -0.94 | 8.69 | -1.70 | -0.95 | -7.94 | 0.10 | 8.66 | -1.06 | -1.25 | -8.83 |
Endoplasmic Reticulum | -1.94 | -19.61 | -3.68 | -3.34 | 20.75 | 1.08 | -24.29 | -14.05 | -15.94 | 16.67 | 0.09 | -33.89 | -13.46 | -15.41 | 25.20 |
Endosome | 0.05 | 0.98 | 0.22 | 0.68 | -3.37 | -13.70 | -3.03 | -2.09 | 12.42 | 1.39 | -13.46 | -3.70 | -3.89 | 10.68 | -0.25 |
Golgi | -2.56 | 2.12 | -1.98 | -0.26 | -4.03 | -4.95 | 8.17 | 3.01 | 7.30 | -4.57 | -3.90 | 9.36 | 2.14 | 5.92 | -6.33 |
Lipid Particles | 1.00 | 2.84 | -0.55 | -3.26 | -7.16 | -1.68 | 9.15 | -1.14 | 0.39 | -9.39 | -0.37 | 10.45 | -1.86 | -1.73 | -11.97 |
Mitochondria | 0.10 | 1.26 | 0.64 | 1.60 | -2.13 | -4.21 | 6.51 | 4.73 | 9.11 | -5.62 | -3.24 | 6.56 | 3.88 | 8.21 | -4.18 |
Mitotic Spindle | -3.32 | -3.14 | -5.93 | -4.48 | -5.21 | -2.53 | -1.40 | -4.08 | -1.89 | -1.60 | -2.45 | -1.14 | -6.04 | -4.38 | -4.69 |
None | 0.14 | 1.37 | -1.71 | -1.87 | -2.39 | 2.58 | 2.08 | 4.61 | 1.76 | 0.69 | 3.15 | 3.59 | 2.03 | -0.47 | -1.31 |
Nuclear Periphery | -0.70 | -7.28 | 0.24 | 1.50 | 7.58 | 1.31 | -6.59 | 2.13 | 0.88 | 6.95 | 0.65 | -9.78 | 2.02 | 1.87 | 10.29 |
Nuclear Periphery Foci | 0.96 | -12.06 | -3.52 | -9.62 | 11.78 | 0.54 | -14.65 | -15.81 | -16.10 | 10.24 | -1.26 | -23.25 | -17.92 | -17.98 | 16.48 |
Nucleolus | -0.32 | 2.96 | 0.07 | 0.51 | -3.80 | -2.90 | 7.95 | 7.24 | 8.93 | -2.32 | -2.44 | 9.46 | 4.74 | 7.26 | -4.82 |
Nucleus | 1.57 | 3.51 | 4.10 | 13.34 | 2.60 | 11.50 | 19.46 | 18.84 | 7.90 | -4.25 | 12.88 | 22.08 | 21.79 | 12.45 | -3.27 |
Peroxisomes | -3.18 | 5.37 | 2.10 | 7.19 | -7.25 | -6.62 | 6.66 | 4.61 | 14.70 | -8.46 | -6.16 | 7.10 | 4.41 | 16.60 | -11.72 |
Vacuole | -2.15 | -2.91 | -11.13 | -15.73 | -12.13 | -17.23 | -8.53 | -29.12 | -14.27 | -10.44 | -19.72 | -12.36 | -35.03 | -20.78 | -15.12 |
Vacuole Periphery | -5.12 | 2.85 | -3.12 | -1.34 | -3.82 | -7.19 | 4.65 | -0.97 | 3.75 | -3.35 | -6.93 | 5.08 | -2.17 | 2.10 | -5.00 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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