Standard name | |
---|---|
Human Ortholog | |
Description | Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 4 | 4 |
Bud | 1 | 3 | 1 | 0 | 1 | 2 | 4 | 4 | 4 | – | 0 | 6 | 1 | 0 | 0 | 1 | 2 | 1 | 23 | 16 | 7 | 13 | 27 | 20 |
Bud Neck | 2 | 0 | 0 | 0 | 1 | 1 | 2 | 5 | 5 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 4 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 10 | 14 | 5 | 1 | 1 | 0 | 0 | 1 | 1 |
Cytoplasm | 300 | 176 | 74 | 64 | 59 | 85 | 98 | 162 | 111 | – | 47 | 85 | 229 | 266 | 291 | 223 | 140 | 166 | 248 | 139 | 62 | 32 | 95 | 89 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | – | 0 | 0 | 2 | 0 | 2 | 16 | 9 | 6 | 0 | 0 | 0 | 1 | 2 | 0 |
Endosome | 4 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 6 | 5 | 3 | 0 | 3 | 5 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 7 | 4 | 4 | 5 | 1 | 14 | 26 | 25 | 49 | – | 21 | 43 | 2 | 0 | 2 | 7 | 6 | 3 | 3 | 4 | 3 | 3 | 11 | 4 |
Nucleus | 20 | 11 | 13 | 1 | 3 | 7 | 9 | 13 | 8 | – | 1 | 6 | 0 | 1 | 0 | 11 | 15 | 7 | 21 | 13 | 11 | 4 | 10 | 5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 4 | 3 | 1 | 0 | 2 | 3 | 2 | 2 | – | 1 | 0 | 1 | 0 | 0 | 6 | 3 | 2 | 9 | 5 | 2 | 3 | 6 | 4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 26 | 20 | 11 | 6 | 23 | 9 |
Vac/Vac Membrane | 11 | 10 | 2 | 0 | 0 | 1 | 1 | 1 | 3 | – | 0 | 4 | 0 | 2 | 3 | 29 | 16 | 18 | 5 | 3 | 3 | 11 | 17 | 18 |
Unique Cell Count | 339 | 205 | 93 | 68 | 64 | 99 | 121 | 194 | 142 | 59 | 119 | 235 | 268 | 296 | 266 | 175 | 196 | 356 | 217 | 106 | 86 | 220 | 173 | |
Labelled Cell Count | 348 | 214 | 100 | 71 | 67 | 113 | 147 | 216 | 183 | 71 | 146 | 235 | 270 | 298 | 311 | 211 | 217 | 356 | 217 | 106 | 86 | 220 | 173 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 5.1 | 4.7 | 4.3 | 5.1 | 3.8 | 4.0 | 4.2 | 3.5 | – | 3.4 | 3.5 | 4.5 | 4.9 | 4.8 | 8.3 | 8.6 | 8.4 | 4.9 | 4.8 | 5.3 |
Std Deviation (1e-4) | 0.9 | 1.1 | 0.8 | 0.9 | 1.2 | 0.8 | 1.1 | 1.1 | 0.5 | – | 1.0 | 0.8 | 1.0 | 1.2 | 1.2 | 2.4 | 2.9 | 2.9 | 1.4 | 1.6 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.13 | 0.12 | -0.3 | -0.23 | -0.16 | -0.44 | – | -0.5 | -0.42 | -0.06 | 0.06 | 0.03 | 0.82 | 0.87 | 0.83 | 0.04 | 0.02 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.6 | 2.2 | 1.2 | 0.3 | 0.8 | -0.3 | – | 0 | -1.4 | 5.5 | 7.0 | 6.6 | 0.9 | 0.1 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -2.8 | -1.9 | -1.6 | -1.6 | -2.0 | -2.2 | – | -2.6 | -2.3 | 0 | 0 | 0 | -3.3 | -1.4 | -3.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.2 | 2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.3567 | -0.7194 | -0.5876 | -0.5172 | -0.4657 | -0.288 | 0.5467 | -0.0005 | 0.0718 | 0.3618 | 0.287 | 0.4991 | 0.4247 | -0.3365 | -0.2262 | 0.5064 | 0.561 | 0.2619 |
Actin | 0.0128 | 0.0004 | 0.0099 | 0.0032 | 0.0536 | 0.0015 | 0.0233 | 0.0023 | 0.0108 | 0.0024 | 0.0134 | 0.0079 | 0.0154 | 0.0003 | 0.0063 | 0.0004 | 0.0025 | 0.0053 |
Bud | 0.0025 | 0.0073 | 0.003 | 0.0003 | 0.0171 | 0.0103 | 0.0095 | 0.0276 | 0.0055 | 0.015 | 0.0112 | 0.0158 | 0.0101 | 0.0102 | 0.0101 | 0.0148 | 0.0054 | 0.0178 |
Bud Neck | 0.0106 | 0.0005 | 0.0008 | 0.0003 | 0.0014 | 0.0224 | 0.0047 | 0.0008 | 0.0019 | 0.0049 | 0.0021 | 0.0246 | 0.0038 | 0.0007 | 0.0008 | 0.0021 | 0.0025 | 0.0137 |
Bud Periphery | 0.0026 | 0.0046 | 0.0036 | 0.0003 | 0.0192 | 0.0113 | 0.0104 | 0.0097 | 0.007 | 0.0155 | 0.0098 | 0.0218 | 0.0096 | 0.0049 | 0.0068 | 0.0165 | 0.0105 | 0.0156 |
Bud Site | 0.0154 | 0.0133 | 0.0011 | 0.0007 | 0.0067 | 0.0052 | 0.0258 | 0.0381 | 0.0195 | 0.0116 | 0.0077 | 0.0064 | 0.0164 | 0.0217 | 0.013 | 0.0016 | 0.0023 | 0.0059 |
Cell Periphery | 0.0008 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0005 | 0.0017 | 0.0009 | 0.0006 | 0.0006 | 0.0005 | 0.0005 | 0.0008 | 0.0005 | 0.0005 | 0.0003 | 0.0003 | 0.0005 |
Cytoplasm | 0.1613 | 0.2042 | 0.1786 | 0.5007 | 0.3142 | 0.1436 | 0.3299 | 0.2288 | 0.3229 | 0.4922 | 0.5348 | 0.2557 | 0.2446 | 0.1917 | 0.223 | 0.4634 | 0.3969 | 0.1767 |
Cytoplasmic Foci | 0.0208 | 0.0085 | 0.0266 | 0.0235 | 0.0182 | 0.0096 | 0.0252 | 0.0079 | 0.0232 | 0.0221 | 0.0374 | 0.0098 | 0.016 | 0.0105 | 0.0064 | 0.0143 | 0.0118 | 0.0066 |
Eisosomes | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0009 | 0.0001 | 0.002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0061 | 0.0021 | 0.0037 | 0.0089 | 0.0043 | 0.0019 | 0.0043 | 0.0019 | 0.0029 | 0.0035 | 0.0032 | 0.0019 | 0.0069 | 0.0014 | 0.0019 | 0.0018 | 0.0052 | 0.0018 |
Endosome | 0.0104 | 0.0019 | 0.0097 | 0.0141 | 0.0465 | 0.0031 | 0.0142 | 0.002 | 0.009 | 0.0234 | 0.0242 | 0.0037 | 0.0131 | 0.0019 | 0.0023 | 0.0066 | 0.0201 | 0.0034 |
Golgi | 0.0039 | 0.0002 | 0.0031 | 0.0063 | 0.0195 | 0.0004 | 0.0057 | 0.0003 | 0.0025 | 0.001 | 0.0043 | 0.0014 | 0.0031 | 0.0002 | 0.0014 | 0.0003 | 0.0057 | 0.0004 |
Lipid Particles | 0.0188 | 0.0003 | 0.0068 | 0.0095 | 0.0358 | 0.0017 | 0.0106 | 0.0006 | 0.0066 | 0.0012 | 0.0049 | 0.0005 | 0.0058 | 0.0005 | 0.0005 | 0.0001 | 0.0118 | 0.0004 |
Mitochondria | 0.0016 | 0.0007 | 0.001 | 0.0499 | 0.0166 | 0.0011 | 0.0074 | 0.0007 | 0.0105 | 0.0013 | 0.0012 | 0.0015 | 0.0027 | 0.0006 | 0.0008 | 0.0004 | 0.0039 | 0.0015 |
None | 0.6038 | 0.7192 | 0.7265 | 0.3306 | 0.3398 | 0.3378 | 0.3914 | 0.5997 | 0.5424 | 0.3293 | 0.2893 | 0.2297 | 0.5035 | 0.7009 | 0.685 | 0.4266 | 0.3677 | 0.3281 |
Nuclear Periphery | 0.0097 | 0.0011 | 0.0025 | 0.0057 | 0.007 | 0.0054 | 0.0053 | 0.0041 | 0.0018 | 0.0048 | 0.0036 | 0.0068 | 0.0152 | 0.0013 | 0.0026 | 0.0014 | 0.0294 | 0.0081 |
Nucleolus | 0.0042 | 0.0033 | 0.0012 | 0.0003 | 0.0016 | 0.038 | 0.0081 | 0.0044 | 0.0026 | 0.0056 | 0.0018 | 0.0391 | 0.0066 | 0.0029 | 0.0025 | 0.0049 | 0.0106 | 0.0306 |
Nucleus | 0.0938 | 0.0267 | 0.0051 | 0.0033 | 0.0222 | 0.3647 | 0.0938 | 0.0519 | 0.0125 | 0.0269 | 0.0268 | 0.3363 | 0.099 | 0.0425 | 0.0253 | 0.0334 | 0.0868 | 0.3403 |
Peroxisomes | 0.006 | 0.0002 | 0.0061 | 0.0363 | 0.0646 | 0.0018 | 0.0101 | 0.0007 | 0.0077 | 0.0022 | 0.0038 | 0.0025 | 0.0046 | 0.0004 | 0.0005 | 0.0004 | 0.0051 | 0.0007 |
Punctate Nuclear | 0.0103 | 0.0018 | 0.008 | 0.0012 | 0.0039 | 0.0334 | 0.008 | 0.0136 | 0.0047 | 0.0274 | 0.0138 | 0.03 | 0.0159 | 0.004 | 0.0071 | 0.0051 | 0.0114 | 0.038 |
Vacuole | 0.0033 | 0.0029 | 0.0021 | 0.0038 | 0.0047 | 0.0054 | 0.0077 | 0.0036 | 0.0043 | 0.0082 | 0.0053 | 0.0035 | 0.0054 | 0.0025 | 0.0026 | 0.0048 | 0.0073 | 0.004 |
Vacuole Periphery | 0.0009 | 0.0003 | 0.0004 | 0.0008 | 0.002 | 0.0007 | 0.0011 | 0.0004 | 0.0007 | 0.001 | 0.0008 | 0.0006 | 0.0013 | 0.0002 | 0.0004 | 0.0006 | 0.0024 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.6786 | 19.6166 | 23.1004 | 19.4148 | 6.1851 | 10.966 | 28.1346 | 36.3773 | 21.5711 | 11.489 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
802 | 726 | 1292 | 113 | 1664 | 586 | 1723 | 1760 | 2466 | 1312 | 3015 | 1873 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 662.67 | 705.30 | 921.26 | 894.10 | 665.46 | 714.51 | 946.26 | 874.44 | 664.55 | 709.41 | 935.55 | 875.63 |
Standard Deviation | 71.85 | 98.65 | 127.08 | 134.35 | 82.91 | 89.46 | 158.69 | 124.39 | 79.50 | 94.76 | 146.51 | 125.10 |
Intensity Change Log 2 | — | 0.089946 | 0.475318 | 0.432146 | — | 0.102602 | 0.507885 | 0.394007 | — | 0.096302 | 0.491727 | 0.413162 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000103 | 0.000356 | 0.000505 | 0.000474 | 0.000178 | 0.000469 | 0.000404 | 0.000101 | 0.000153 | 0.000407 | 0.000447 | 0.000124 |
Bud Neck | 0.009358 | 0.025468 | 0.019143 | 0.006553 | 0.021419 | 0.036341 | 0.015876 | 0.035409 | 0.017497 | 0.030324 | 0.017276 | 0.033668 |
Bud Site | 0.006177 | 0.017441 | 0.008092 | 0.008802 | 0.008222 | 0.019390 | 0.008792 | 0.014895 | 0.007557 | 0.018312 | 0.008492 | 0.014528 |
Cell Periphery | 0.000249 | 0.000221 | 0.000203 | 0.000251 | 0.000267 | 0.000243 | 0.000357 | 0.000185 | 0.000261 | 0.000231 | 0.000291 | 0.000189 |
Cytoplasm | 0.541626* | 0.443304* | 0.260040* | 0.426752* | 0.489363* | 0.373723* | 0.418762* | 0.633819* | 0.506360* | 0.412226* | 0.350746* | 0.621327* |
Cytoplasmic Foci | 0.113108 | 0.153375 | 0.003718 | 0.050429 | 0.137511 | 0.236214* | 0.012260 | 0.005873 | 0.129575 | 0.190375* | 0.008600 | 0.008561 |
Eisosomes | 0.000087 | 0.000088 | 0.000020 | 0.000084 | 0.000088 | 0.000176 | 0.000038 | 0.000011 | 0.000087 | 0.000128 | 0.000030 | 0.000015 |
Endoplasmic Reticulum | 0.000407 | 0.000948 | 0.002757 | 0.003852 | 0.001042 | 0.000759 | 0.002185 | 0.000367 | 0.000836 | 0.000864 | 0.002430 | 0.000577 |
Endosome | 0.005315 | 0.027575 | 0.001272 | 0.011089 | 0.014522 | 0.037375 | 0.002110 | 0.000782 | 0.011527 | 0.031952 | 0.001751 | 0.001404 |
Golgi | 0.000620 | 0.002162 | 0.000194 | 0.000228 | 0.000986 | 0.005136 | 0.000243 | 0.000254 | 0.000867 | 0.003490 | 0.000222 | 0.000252 |
Lipid Particles | 0.002866 | 0.005138 | 0.000548 | 0.003005 | 0.005354 | 0.006206 | 0.000648 | 0.000125 | 0.004545 | 0.005615 | 0.000605 | 0.000299 |
Mitochondria | 0.001761 | 0.004164 | 0.001346 | 0.000551 | 0.002005 | 0.009464 | 0.001024 | 0.000680 | 0.001925 | 0.006531 | 0.001162 | 0.000672 |
Mitotic Spindle | 0.001185 | 0.010330 | 0.008451 | 0.013673 | 0.001862 | 0.009001 | 0.018623 | 0.016435 | 0.001642 | 0.009737 | 0.014264 | 0.016268 |
None | 0.015920 | 0.010468 | 0.009987 | 0.003072 | 0.019170 | 0.009652 | 0.016141 | 0.010982 | 0.018113 | 0.010103 | 0.013504 | 0.010504 |
Nuclear Periphery | 0.000305 | 0.000659 | 0.001381 | 0.001277 | 0.000579 | 0.000330 | 0.001236 | 0.000547 | 0.000490 | 0.000512 | 0.001298 | 0.000591 |
Nuclear Periphery Foci | 0.000302 | 0.000974 | 0.002100 | 0.005542 | 0.000946 | 0.000815 | 0.001209 | 0.000154 | 0.000737 | 0.000903 | 0.001591 | 0.000479 |
Nucleolus | 0.014131 | 0.012350 | 0.009377 | 0.001761 | 0.013375 | 0.015023 | 0.010387 | 0.006316 | 0.013621 | 0.013544 | 0.009954 | 0.006041 |
Nucleus | 0.248657* | 0.168163* | 0.640187* | 0.293703* | 0.203485* | 0.124580 | 0.416857* | 0.258032* | 0.218176* | 0.148696* | 0.512559* | 0.260184* |
Peroxisomes | 0.002158 | 0.010052 | 0.000252 | 0.000811 | 0.003245 | 0.017721 | 0.000385 | 0.000208 | 0.002891 | 0.013477 | 0.000328 | 0.000244 |
Vacuole | 0.035098 | 0.104314 | 0.030101 | 0.167697 | 0.075160 | 0.094445 | 0.072047 | 0.014383 | 0.062131 | 0.099906 | 0.054072 | 0.023632 |
Vacuole Periphery | 0.000568 | 0.002450 | 0.000327 | 0.000395 | 0.001221 | 0.002936 | 0.000418 | 0.000442 | 0.001009 | 0.002667 | 0.000379 | 0.000439 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.22 | -13.44 | -3.98 | -2.33 | -0.72 | -3.78 | -9.84 | 3.45 | 5.04 | 14.71* | -6.13 | -15.52 | 0.59 | 6.61 | 17.16* |
Bud Neck | -5.98 | -6.46 | -0.24 | 4.04 | 3.68 | -3.88 | 0.01 | -6.71 | -1.16 | -7.12 | -5.51 | -2.86 | -8.49 | -2.76 | -6.84 |
Bud Site | -4.04 | -1.56 | -1.56 | 1.80 | -0.69 | -5.13 | -1.39 | -5.44 | 1.81 | -4.66 | -6.27 | -1.98 | -5.98 | 1.73 | -4.94 |
Cell Periphery | 0.51 | 0.89 | 0.10 | -0.52 | -1.27 | 0.68 | 0.06 | 3.35 | 1.63 | 3.63 | 0.92 | 0.89 | 3.02 | 1.12 | 3.16 |
Cytoplasm | 6.90 | 24.87 | 6.37 | 2.44 | -5.20 | 8.24 | 13.55 | -11.55 | -16.86 | -24.90 | 9.53 | 26.09 | -9.32 | -16.81 | -32.85 |
Cytoplasmic Foci | -4.77 | 21.25 | 9.52 | 13.16 | -6.33 | -9.76 | 30.03 | 30.96 | 25.92 | 3.69 | -9.21 | 36.81 | 36.06 | 33.39 | -1.59 |
Eisosomes | -0.08 | 6.35 | -0.27 | -0.26 | -6.63 | -6.29 | 11.80 | 21.38 | 12.42 | 22.60 | -5.05 | 12.90 | 18.07 | 16.87 | 15.81 |
Endoplasmic Reticulum | -5.79 | -17.20 | -6.16 | -5.30 | -1.20 | 2.02 | -6.61 | 5.95 | 4.34 | 9.86 | -0.56 | -13.69 | 1.85 | 2.61 | 15.11 |
Endosome | -8.44 | 5.89 | -2.17 | 6.23 | -4.90 | -6.49 | 14.04 | 15.50 | 10.90 | 6.42 | -9.32 | 15.17 | 15.79 | 14.87 | 2.98 |
Golgi | -4.01 | 1.88 | 1.67 | 5.76 | -0.60 | -4.21 | 7.81 | 7.22 | 4.98 | -0.12 | -5.39 | 6.83 | 6.22 | 6.76 | -0.35 |
Lipid Particles | -2.61 | 7.27 | -0.17 | 2.26 | -4.60 | -0.71 | 11.03 | 12.60 | 6.18 | 15.71 | -1.41 | 12.75 | 14.24 | 8.53 | 9.29 |
Mitochondria | -2.14 | 0.33 | 1.24 | 4.63 | 4.51 | -5.00 | 4.71 | 7.18 | 5.95 | 6.67 | -5.51 | 2.22 | 4.27 | 7.52 | 9.81 |
Mitotic Spindle | -3.62 | -6.43 | -2.37 | -0.83 | -0.64 | -3.27 | -10.31 | -7.98 | -3.08 | 2.39 | -4.74 | -12.09 | -8.51 | -2.89 | 0.82 |
None | 2.15 | 3.37 | 6.22 | 4.28 | 9.39 | 3.99 | 3.90 | 4.71 | -0.81 | 0.66 | 4.57 | 5.33 | 5.57 | -0.43 | -0.05 |
Nuclear Periphery | -2.42 | -20.40 | -7.60 | -4.34 | 0.84 | 4.31 | -15.79 | -1.85 | -7.58 | 16.07 | -0.47 | -23.71 | -5.91 | -2.23 | 19.76 |
Nuclear Periphery Foci | -3.66 | -7.60 | -4.28 | -3.67 | -2.56 | 0.43 | -1.43 | 5.68 | 4.24 | 8.95 | -1.25 | -5.95 | 1.87 | 2.81 | 7.57 |
Nucleolus | 0.13 | 1.60 | 4.84 | 5.16 | 5.07 | -0.82 | 1.11 | 2.78 | 2.56 | 1.73 | -0.52 | 1.94 | 3.60 | 3.48 | 2.05 |
Nucleus | 6.50 | -31.83 | -1.29 | -4.17 | 12.37 | 7.88 | -23.98 | -8.11 | -14.48 | 16.90 | 8.95 | -39.32 | -7.07 | -14.76 | 30.05 |
Peroxisomes | -6.21 | 2.82 | 1.90 | 8.35 | -2.54 | -7.30 | 8.33 | 9.12 | 9.08 | 2.56 | -9.46 | 8.21 | 8.57 | 12.49 | 1.78 |
Vacuole | -12.29 | -4.25 | -8.82 | -5.32 | -7.94 | -3.47 | -7.35 | 17.78 | 14.04 | 19.37 | -9.28 | -7.08 | 10.03 | 16.02 | 14.83 |
Vacuole Periphery | -4.19 | 3.29 | 2.60 | 4.76 | -0.31 | -3.66 | 10.83 | 9.34 | 5.48 | -1.77 | -5.06 | 11.33 | 8.80 | 7.06 | -2.06 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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