Standard name | |
---|---|
Human Ortholog | |
Description | Minor isoform of pyruvate decarboxylase; key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 4 | – | 6 | 2 | 7 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 1 | 2 | 5 | 7 | 4 | 12 | – | 7 | 9 | 4 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 2 | 0 | 1 | 4 | – | 0 | 3 | 3 | 2 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 201 | 180 | 170 | 304 | 280 | 347 | 470 | – | 263 | 262 | 354 | 478 | 27 | 27 | 95 | 62 | 90 | 118 | 169 | 166 | 125 | 13 | 35 | 41 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 5 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1 | 2 | 2 | 1 | 3 | 5 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 5 | 8 | 2 | 2 | 1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 20 | 2 | 1 | 5 | 1 | 40 | 48 | – | 90 | 64 | 206 | 126 | 2 | 0 | 0 | 4 | 1 | 1 | 17 | 7 | 14 | 0 | 0 | 1 |
Nucleus | 15 | 19 | 14 | 26 | 17 | 16 | 14 | – | 6 | 7 | 9 | 3 | 3 | 3 | 9 | 24 | 29 | 19 | 30 | 35 | 22 | 5 | 2 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 1 | 1 | 1 | 5 | 12 | – | 8 | 6 | 8 | 15 | 0 | 0 | 0 | 0 | 2 | 0 | 6 | 1 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 29 | 34 | 9 | 9 | 28 | 16 | 12 | – | 7 | 10 | 5 | 12 | 4 | 0 | 5 | 70 | 64 | 88 | 16 | 18 | 20 | 1 | 3 | 4 |
Unique Cell Count | 248 | 223 | 194 | 342 | 319 | 399 | 544 | 325 | 331 | 504 | 585 | 35 | 30 | 105 | 144 | 166 | 205 | 262 | 244 | 201 | 24 | 45 | 56 | |
Labelled Cell Count | 267 | 240 | 199 | 354 | 336 | 436 | 582 | 388 | 370 | 605 | 658 | 37 | 30 | 110 | 172 | 199 | 238 | 262 | 244 | 201 | 24 | 45 | 56 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 4.9 | 5.5 | 4.9 | 5.3 | 5.1 | 4.4 | – | 3.9 | 3.9 | 3.4 | 3.6 | 4.5 | 5.3 | 6.8 | 8.7 | 8.8 | 8.4 | – | – | – |
Std Deviation (1e-4) | 2.0 | 1.5 | 3.8 | 1.5 | 1.4 | 13.1 | 1.3 | – | 5.7 | 1.3 | 1.0 | 1.0 | 1.1 | 1.8 | 10.6 | 3.0 | 3.6 | 2.8 | – | – | – |
Intensity Change (Log2) | – | – | – | -0.15 | -0.05 | -0.11 | -0.32 | – | -0.49 | -0.49 | -0.7 | -0.61 | -0.29 | -0.04 | 0.31 | 0.66 | 0.68 | 0.61 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.4 | 0 | -0.2 | -0.4 | – | -2.0 | -2.5 | -4.8 | -1.9 | -1.6 | 0.4 | 0.7 | -8.7 | -7.1 | -6.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.2 | -0.9 | -1.7 | -2.9 | – | -3.1 | -2.9 | -3.6 | 0 | 0 | 0 | 0.4 | 2.7 | 3.0 | 0.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.2 | 1.8 | -0.4 | -1.8 | – | -1.6 | -1.0 | 0 | -1.9 | 0 | 0 | 0 | 9.4 | 8.0 | 8.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.6658 | -0.0589 | -0.2176 | -0.0511 | -0.8824 | -0.4051 | -0.2904 | -0.3157 | -0.3881 | -0.7067 | -1.1147 | -0.8973 | -1.1379 | -1.0205 | -1.2034 | -1.3497 | -0.6651 | -1.1315 |
Actin | 0.0012 | 0.0004 | 0.0002 | 0.0001 | 0.0009 | 0.0007 | 0.0284 | 0.0001 | 0.0115 | 0.0003 | 0.0106 | 0.0016 | 0.0343 | 0.0001 | 0.0146 | 0.0495 | 0.0412 | 0.0074 |
Bud | 0.0021 | 0.0007 | 0.0003 | 0.0012 | 0.0075 | 0.0007 | 0.0008 | 0.0007 | 0.0009 | 0.0002 | 0.0015 | 0.0032 | 0.0007 | 0.0005 | 0.0022 | 0.0002 | 0.0025 | 0.0003 |
Bud Neck | 0.0006 | 0.0005 | 0.0002 | 0.0008 | 0.0016 | 0.0021 | 0.0051 | 0.0002 | 0.0008 | 0.0001 | 0.0018 | 0.0041 | 0.0039 | 0.0001 | 0.003 | 0.0003 | 0.0019 | 0.0092 |
Bud Periphery | 0.0014 | 0.0011 | 0.0002 | 0.0016 | 0.0038 | 0.001 | 0.0016 | 0.0003 | 0.0013 | 0.0002 | 0.0018 | 0.0015 | 0.0017 | 0.0002 | 0.0015 | 0.0002 | 0.0054 | 0.0002 |
Bud Site | 0.0029 | 0.0015 | 0.0021 | 0.0002 | 0.0038 | 0.0002 | 0.0045 | 0.0042 | 0.005 | 0.0008 | 0.0007 | 0.0003 | 0.0047 | 0.0038 | 0.0096 | 0.0004 | 0.0021 | 0.0002 |
Cell Periphery | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0886 | 0.1502 | 0.0782 | 0.054 | 0.0982 | 0.1071 | 0.0488 | 0.0761 | 0.0805 | 0.0391 | 0.0821 | 0.1021 | 0.0513 | 0.1599 | 0.1218 | 0.203 | 0.1417 | 0.1784 |
Cytoplasmic Foci | 0.0129 | 0.0119 | 0.0047 | 0.0033 | 0.0044 | 0.0108 | 0.0336 | 0.0013 | 0.0258 | 0.0076 | 0.0052 | 0.0164 | 0.0335 | 0.0051 | 0.0079 | 0.0064 | 0.0146 | 0.0188 |
Eisosomes | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0006 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0015 | 0.0021 | 0.0011 | 0.0006 | 0.0012 | 0.0008 | 0.0031 | 0.0024 | 0.0009 | 0.0006 | 0.0033 | 0.001 | 0.0027 | 0.0012 | 0.0018 | 0.0008 | 0.004 | 0.0021 |
Endosome | 0.0063 | 0.0017 | 0.0033 | 0.0007 | 0.0007 | 0.0014 | 0.0418 | 0.001 | 0.0186 | 0.0408 | 0.0243 | 0.0032 | 0.0119 | 0.0012 | 0.0051 | 0.001 | 0.0159 | 0.0029 |
Golgi | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0097 | 0 | 0.004 | 0.0084 | 0.0055 | 0.0004 | 0.0051 | 0 | 0.0015 | 0.0009 | 0.0107 | 0.001 |
Lipid Particles | 0.0027 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0153 | 0.0001 | 0.0053 | 0.0014 | 0.0104 | 0.0011 | 0.0228 | 0.0001 | 0.002 | 0.0007 | 0.0094 | 0.0022 |
Mitochondria | 0.0033 | 0.0005 | 0.0007 | 0.001 | 0.0004 | 0.0005 | 0.0082 | 0.0002 | 0.0079 | 0.0053 | 0.0787 | 0.0011 | 0.0022 | 0.0001 | 0.0014 | 0.001 | 0.0047 | 0.0004 |
None | 0.7182 | 0.7015 | 0.7604 | 0.6903 | 0.7384 | 0.7223 | 0.6543 | 0.8397 | 0.737 | 0.7291 | 0.6892 | 0.7796 | 0.7423 | 0.7652 | 0.7219 | 0.7212 | 0.6762 | 0.6703 |
Nuclear Periphery | 0.0056 | 0.0051 | 0.0039 | 0.0024 | 0.0016 | 0.0022 | 0.0201 | 0.0048 | 0.003 | 0.0026 | 0.0138 | 0.0026 | 0.0065 | 0.0013 | 0.0054 | 0.0008 | 0.0029 | 0.0035 |
Nucleolus | 0.0024 | 0.0012 | 0.0014 | 0.0019 | 0.002 | 0.0019 | 0.0051 | 0.0007 | 0.0019 | 0.0012 | 0.0022 | 0.0025 | 0.0015 | 0.0003 | 0.0027 | 0.0002 | 0.0005 | 0.0011 |
Nucleus | 0.1267 | 0.1051 | 0.1223 | 0.1847 | 0.1256 | 0.1247 | 0.0715 | 0.0658 | 0.0721 | 0.1485 | 0.0513 | 0.0489 | 0.034 | 0.0559 | 0.0771 | 0.0032 | 0.0479 | 0.0826 |
Peroxisomes | 0.0056 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0167 | 0 | 0.0079 | 0.001 | 0.0023 | 0.0021 | 0.0278 | 0 | 0.0013 | 0.0008 | 0.0081 | 0.0007 |
Punctate Nuclear | 0.0144 | 0.0128 | 0.0184 | 0.0558 | 0.0089 | 0.0219 | 0.0247 | 0.0015 | 0.0125 | 0.0094 | 0.0073 | 0.0265 | 0.0108 | 0.0028 | 0.0172 | 0.0083 | 0.0082 | 0.0177 |
Vacuole | 0.0018 | 0.0022 | 0.0013 | 0.0009 | 0.0005 | 0.0005 | 0.0043 | 0.0007 | 0.0016 | 0.0023 | 0.004 | 0.0012 | 0.0012 | 0.0015 | 0.001 | 0.0003 | 0.0011 | 0.0007 |
Vacuole Periphery | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0021 | 0.0001 | 0.0003 | 0.001 | 0.0034 | 0.0002 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0005 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 462.3604 | 568.3905 | 805.9057 | 315.6437 | 532.252 | 579.4229 | 449.2968 | 441.59 | 608.1423 | 300.9746 |
Translational Efficiency | 0.7196 | 0.6469 | 0.4844 | 1.3435 | 0.7045 | 0.6551 | 1.1218 | 1.5042 | 0.9541 | 1.4827 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1100 | 298 | 175 | 793 | 1724 | 1781 | 207 | 518 | 2824 | 2079 | 382 | 1311 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 638.29 | 751.65 | 3954.07 | 943.88 | 695.41 | 715.05 | 1023.61 | 951.22 | 673.16 | 720.30 | 2366.10 | 946.78 |
Standard Deviation | 140.22 | 132.15 | 6377.01 | 490.83 | 96.84 | 125.04 | 237.57 | 293.97 | 118.99 | 126.73 | 4559.85 | 424.13 |
Intensity Change Log 2 | — | 0.235849 | 2.631054 | 0.564391 | — | 0.040180 | 0.557730 | 0.451915 | — | 0.137140 | 1.900039 | 0.506840 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000166 | 0.000805 | 0.001053 | 0.003662 | 0.000083 | 0.000568 | 0.000974 | 0.000619 | 0.000115 | 0.000602 | 0.001010 | 0.002460 |
Bud Neck | 0.008365 | 0.027272 | 0.006366 | 0.013151 | 0.007955 | 0.028215 | 0.005338 | 0.006731 | 0.008114 | 0.028080 | 0.005809 | 0.010615 |
Bud Site | 0.005054 | 0.011940 | 0.004630 | 0.023519 | 0.003813 | 0.011499 | 0.005752 | 0.015873 | 0.004296 | 0.011563 | 0.005238 | 0.020498 |
Cell Periphery | 0.000500 | 0.000201 | 0.002227 | 0.000280 | 0.000147 | 0.000160 | 0.000221 | 0.000249 | 0.000284 | 0.000166 | 0.001140 | 0.000267 |
Cytoplasm | 0.439487* | 0.283429* | 0.141953* | 0.326104* | 0.467931* | 0.398090* | 0.166774* | 0.339004* | 0.456851* | 0.381655* | 0.155403* | 0.331201* |
Cytoplasmic Foci | 0.074197 | 0.189668* | 0.021258 | 0.015864 | 0.120889 | 0.144586 | 0.006227 | 0.031298 | 0.102702 | 0.151048 | 0.013113 | 0.021962 |
Eisosomes | 0.000112 | 0.000148 | 0.000097 | 0.000045 | 0.000070 | 0.000100 | 0.000085 | 0.000075 | 0.000086 | 0.000107 | 0.000091 | 0.000056 |
Endoplasmic Reticulum | 0.001876 | 0.001924 | 0.005413 | 0.004946 | 0.000602 | 0.000841 | 0.010077 | 0.002657 | 0.001098 | 0.000996 | 0.007940 | 0.004042 |
Endosome | 0.002922 | 0.017848 | 0.003378 | 0.006812 | 0.005519 | 0.012971 | 0.003780 | 0.013978 | 0.004508 | 0.013670 | 0.003596 | 0.009644 |
Golgi | 0.000475 | 0.003499 | 0.000561 | 0.003395 | 0.001509 | 0.003369 | 0.000137 | 0.007254 | 0.001106 | 0.003388 | 0.000331 | 0.004920 |
Lipid Particles | 0.005540 | 0.007835 | 0.002332 | 0.001129 | 0.004027 | 0.005379 | 0.002351 | 0.002363 | 0.004617 | 0.005731 | 0.002342 | 0.001617 |
Mitochondria | 0.003713 | 0.008188 | 0.001848 | 0.003158 | 0.005244 | 0.013435 | 0.000924 | 0.015015 | 0.004647 | 0.012683 | 0.001347 | 0.007843 |
Mitotic Spindle | 0.001908 | 0.009574 | 0.004550 | 0.030536 | 0.003061 | 0.015898 | 0.010323 | 0.021600 | 0.002612 | 0.014992 | 0.007679 | 0.027005 |
None | 0.028323 | 0.004316 | 0.004179 | 0.008638 | 0.008555 | 0.006317 | 0.006651 | 0.008500 | 0.016255 | 0.006030 | 0.005518 | 0.008584 |
Nuclear Periphery | 0.000917 | 0.000763 | 0.001724 | 0.001821 | 0.000521 | 0.000919 | 0.002476 | 0.001473 | 0.000675 | 0.000896 | 0.002131 | 0.001683 |
Nuclear Periphery Foci | 0.000905 | 0.000979 | 0.003762 | 0.001402 | 0.000398 | 0.000364 | 0.002082 | 0.002673 | 0.000595 | 0.000452 | 0.002852 | 0.001904 |
Nucleolus | 0.003139 | 0.002604 | 0.001506 | 0.000852 | 0.001626 | 0.002609 | 0.000958 | 0.001822 | 0.002215 | 0.002608 | 0.001209 | 0.001235 |
Nucleus | 0.397923* | 0.332845* | 0.733446* | 0.481146* | 0.342970* | 0.298813* | 0.661966* | 0.406324* | 0.364375* | 0.303691* | 0.694712* | 0.451582* |
Peroxisomes | 0.001516 | 0.034904 | 0.000967 | 0.001859 | 0.003310 | 0.010878 | 0.001046 | 0.001210 | 0.002611 | 0.014322 | 0.001010 | 0.001603 |
Vacuole | 0.022203 | 0.059889 | 0.058307 | 0.066325 | 0.020900 | 0.043184 | 0.111629 | 0.113923 | 0.021408 | 0.045578 | 0.087201 | 0.085132 |
Vacuole Periphery | 0.000759 | 0.001369 | 0.000442 | 0.005356 | 0.000871 | 0.001805 | 0.000230 | 0.007360 | 0.000827 | 0.001743 | 0.000327 | 0.006148 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.85 | -8.06 | -2.65 | -1.53 | -0.63 | -1.47 | -8.20 | -8.07 | -0.21 | 3.69 | -1.68 | -11.59 | -3.31 | -1.56 | -0.10 |
Bud Neck | -3.91 | -0.07 | -1.11 | 3.51 | -1.05 | -10.59 | -0.88 | 0.62 | 10.74 | 1.31 | -11.38 | -0.76 | -0.83 | 10.55 | -0.02 |
Bud Site | -2.21 | -0.52 | -4.25 | -1.14 | -3.90 | -5.96 | -2.35 | -3.24 | 0.39 | -1.57 | -6.27 | -2.36 | -5.48 | -1.17 | -4.03 |
Cell Periphery | 5.35 | -1.51 | 5.66 | -0.77 | 1.76 | -0.78 | -4.05 | -1.83 | -1.25 | 2.65 | 5.66 | -1.65 | 3.56 | -3.17 | 1.81 |
Cytoplasm | 8.95 | 19.01 | 15.79 | 2.43 | -6.60 | 7.28 | 26.31 | 16.06 | 10.74 | -8.20 | 9.04 | 32.28 | 23.67 | 14.24 | -10.55 |
Cytoplasmic Foci | -8.91 | 9.19 | 17.52 | 14.48 | 2.60 | -3.65 | 28.09 | 22.92 | 25.29 | -4.76 | -9.11 | 25.26 | 29.47 | 30.95 | 0.18 |
Eisosomes | -1.86 | 0.53 | 3.11 | 5.94 | 2.59 | -4.34 | -4.37 | -1.02 | 3.39 | 3.69 | -2.49 | -2.07 | 2.54 | 6.03 | 4.53 |
Endoplasmic Reticulum | -0.49 | -4.38 | -10.76 | -9.55 | -0.87 | -2.88 | -11.58 | -10.06 | -9.36 | 7.38 | 0.85 | -11.76 | -16.13 | -16.90 | 4.85 |
Endosome | -5.83 | -0.60 | -0.44 | 5.63 | 0.17 | -6.77 | 2.17 | 0.12 | 4.95 | -1.09 | -9.50 | 1.50 | 0.62 | 9.00 | -0.53 |
Golgi | -3.63 | -0.90 | -1.28 | 2.40 | -0.99 | -3.29 | 4.64 | -0.60 | 1.03 | -1.95 | -4.90 | 3.93 | -0.76 | 2.80 | -2.29 |
Lipid Particles | -1.58 | 3.51 | 8.79 | 5.03 | 2.25 | -2.30 | 3.01 | 5.21 | 8.47 | 2.47 | -2.12 | 4.37 | 9.68 | 10.70 | 3.41 |
Mitochondria | -1.88 | 2.05 | 2.24 | 2.98 | 0.65 | -4.56 | 3.89 | 0.06 | 3.45 | -2.12 | -5.49 | 4.30 | 1.47 | 6.44 | -1.99 |
Mitotic Spindle | -1.98 | -1.74 | -5.80 | -1.72 | -4.64 | -5.02 | -3.00 | -4.62 | -1.59 | 0.20 | -5.64 | -3.22 | -7.28 | -2.11 | -1.33 |
None | 7.90 | 8.36 | 7.57 | -1.69 | -3.62 | 2.38 | 3.80 | 0.43 | -1.67 | -3.03 | 7.60 | 9.18 | 7.10 | -1.06 | -4.21 |
Nuclear Periphery | 0.91 | -6.22 | -13.02 | -10.47 | -3.97 | -1.93 | -12.83 | -12.76 | -6.82 | 2.81 | -1.23 | -13.94 | -18.92 | -9.46 | -0.10 |
Nuclear Periphery Foci | -0.25 | -3.85 | -3.28 | -2.29 | 2.93 | 0.69 | -4.60 | -3.27 | -3.38 | 1.65 | 1.95 | -5.97 | -5.68 | -6.84 | 3.35 |
Nucleolus | 0.88 | 3.37 | 4.64 | 5.53 | 2.73 | -3.35 | 2.99 | 2.32 | 5.14 | 0.05 | -1.27 | 4.38 | 5.72 | 8.75 | 2.01 |
Nucleus | 2.88 | -12.60 | -6.32 | -6.97 | 8.38 | 4.09 | -12.29 | -5.72 | -8.21 | 7.08 | 6.48 | -17.69 | -9.80 | -14.24 | 10.39 |
Peroxisomes | -6.13 | 2.55 | 1.94 | 6.27 | -0.02 | -7.42 | 4.55 | 5.47 | 11.81 | 0.30 | -10.26 | 5.20 | 5.23 | 12.41 | 0.07 |
Vacuole | -5.91 | -4.94 | -17.16 | -10.40 | -5.73 | -9.87 | -9.53 | -16.66 | -14.60 | -1.75 | -10.65 | -10.51 | -24.18 | -20.33 | -4.21 |
Vacuole Periphery | -1.77 | 2.13 | -1.23 | -0.50 | -1.68 | -3.06 | 3.19 | -1.74 | -0.61 | -2.57 | -3.75 | 3.70 | -1.94 | -0.37 | -2.87 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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