Standard name
Human Ortholog
Description mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; TIS11 has a paralog, CTH1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.93 0.98 0.98 0.97 0.95 0.9 0.91 0.78 0.84 0.79 0.8 0.96 0.94 0.96 – 0.95 0.89 0.81 0.82 0.8 0.62 0.56 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.09 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0.06 0.1 0.15 0.07 0.39 0.24 0.31 0.3 0 0 0 – 0.07 0 0.09 0.05 0.06 0.18 0.24 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.08 – 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 2 1 0 0 0 0 1 0 – 0 0 1 1 5 1 3 3
Bud 0 1 0 1 0 1 6 3 8 2 7 6 0 1 0 – 0 0 0 0 11 3 4 9
Bud Neck 0 1 13 4 7 2 9 16 2 5 6 8 0 0 0 – 0 0 0 1 2 0 0 2
Bud Site 0 0 0 0 0 0 0 0 1 3 0 4 0 0 0 – 0 0 – – – – – –
Cell Periphery 2 0 4 2 2 1 2 1 2 1 3 1 0 0 0 – 1 0 0 0 2 0 1 1
Cytoplasm 146 198 484 365 352 464 446 642 405 427 436 474 131 207 227 – 95 117 138 180 387 136 131 144
Endoplasmic Reticulum 5 4 0 0 0 0 1 0 1 0 0 0 0 2 0 – 9 12 0 0 2 3 3 1
Endosome 1 0 0 0 1 2 0 2 2 0 3 2 0 0 0 – 1 5 3 5 5 5 5 7
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – 1 0 2 1 2 4 7 6
Mitochondria 7 13 0 10 22 48 75 49 201 122 170 181 0 0 0 – 7 4 14 10 28 40 55 39
Nucleus 0 0 2 1 3 1 8 7 6 4 3 2 2 0 1 – 2 0 0 0 1 1 1 0
Nuclear Periphery 0 2 0 0 0 0 1 0 1 2 0 2 0 0 0 – 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 1 0 0 1 2 0 1 0 0 0 0 0 – 1 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 2 0 0 0 1 1 0 0 0 0 – 0 0 0 1 0 3 3 7
SpindlePole 0 0 2 1 1 3 1 11 5 6 6 13 0 1 0 – 0 0 1 2 10 6 5 4
Vac/Vac Membrane 2 3 3 6 1 6 0 6 5 6 7 6 9 18 19 – 0 11 3 2 5 2 4 2
Unique Cell Count 159 212 492 372 362 490 497 703 517 509 549 596 137 220 237 100 132 171 219 482 221 236 237
Labelled Cell Count 163 222 509 391 390 530 550 741 640 580 642 699 142 230 247 117 149 171 219 482 221 236 237


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.2 5.3 5.0 4.2 3.9 3.2 4.4 3.2 3.7 3.6 3.8 16.5 14.5 13.4 4.5 5.9 6.0 6.0 6.2 6.4
Std Deviation (1e-4) 0.6 1.4 1.9 2.1 1.4 1.1 1.2 1.6 1.0 1.1 1.0 1.0 4.5 3.8 3.1 2.3 2.6 2.5 2.4 1.9 2.2
Intensity Change (Log2) – – – -0.07 -0.32 -0.43 -0.73 -0.26 -0.72 -0.5 -0.53 -0.48 1.65 1.46 1.35 -0.22 0.16 0.19 0.18 0.24 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5546 5.1634 5.3958 4.3528 4.7098 5.2445 5.4977 3.6722 4.0177 4.8982 4.1924 4.4022 1.4463 1.7807 1.3562 1.5871 2.2592 1.8476
Actin 0.0232 0.0044 0.0232 0.0007 0.0056 0.0077 0.2615 0.0001 0.041 0.0052 0.0021 0.0198 0.0366 0.0017 0.0131 0.0129 0.045 0.0036
Bud 0.0004 0.0026 0.0015 0.0001 0.0003 0.0001 0.0009 0.0006 0.0004 0.0005 0.0001 0.0002 0.0003 0.0101 0.0002 0.0001 0.0006 0
Bud Neck 0.0013 0.0002 0.0005 0.0002 0.0005 0.001 0.0065 0.0001 0.0008 0.0013 0.0001 0.0005 0.0002 0.0001 0.0002 0.0001 0.0007 0.0002
Bud Periphery 0.0005 0.0012 0.0016 0.0001 0.0002 0.0001 0.0009 0.0008 0.0005 0.0007 0.0002 0.0002 0.0007 0.0014 0.0002 0.0002 0.0008 0.0001
Bud Site 0.002 0.0048 0.0019 0.0001 0.0008 0.0001 0.0131 0.0003 0.0018 0.0012 0 0.0002 0.0007 0.003 0.0006 0.0001 0.0029 0
Cell Periphery 0.0003 0.0003 0.0002 0.0001 0 0 0.0004 0.0002 0.0002 0.0002 0 0 0.0002 0.0002 0.0001 0 0.0001 0
Cytoplasm 0.538 0.7793 0.632 0.7386 0.6532 0.7883 0.171 0.4724 0.3333 0.2116 0.3002 0.3769 0.1371 0.2294 0.2274 0.1952 0.1957 0.3491
Cytoplasmic Foci 0.0271 0.0057 0.0102 0.0114 0.0281 0.0145 0.0506 0.0031 0.0165 0.0151 0.0553 0.0075 0.0185 0.0124 0.0158 0.0107 0.067 0.0038
Eisosomes 0.0005 0.0001 0.0002 0 0 0 0.0009 0.0001 0.0004 0.0001 0.0001 0.0001 0.0008 0.0001 0.0002 0.0003 0.0002 0.0001
Endoplasmic Reticulum 0.0106 0.0035 0.0054 0.0026 0.0025 0.0033 0.0039 0.0021 0.0052 0.0028 0.0009 0.0013 0.0033 0.0023 0.0021 0.0026 0.0012 0.0013
Endosome 0.0208 0.0027 0.0104 0.0428 0.0163 0.0044 0.0393 0.0022 0.01 0.0431 0.0031 0.0042 0.0132 0.0042 0.0135 0.0052 0.0397 0.0022
Golgi 0.0134 0.001 0.0086 0.0023 0.0363 0.0025 0.0114 0.0001 0.0074 0.0375 0.002 0.0059 0.0081 0.0007 0.0079 0.0045 0.0362 0.0007
Lipid Particles 0.0118 0.0003 0.0097 0.0003 0.0077 0.0002 0.0212 0.0001 0.014 0.0111 0.0013 0.0003 0.0109 0.0027 0.0085 0.0027 0.0531 0.0009
Mitochondria 0.0097 0.0004 0.0109 0.0011 0.0071 0.0009 0.0035 0.0015 0.0023 0.0264 0.0021 0.0021 0.0181 0.0003 0.0053 0.0014 0.0099 0.0007
None 0.3023 0.1819 0.2674 0.191 0.2089 0.1496 0.2854 0.5073 0.5377 0.5818 0.5959 0.5784 0.7191 0.7189 0.6807 0.7576 0.4239 0.6323
Nuclear Periphery 0.0059 0.0022 0.0022 0.0009 0.0008 0.0042 0.0477 0.001 0.0071 0.0026 0.0002 0.0003 0.0079 0.0019 0.0024 0.0008 0.0016 0.0023
Nucleolus 0.0005 0.0002 0.0001 0 0 0.0002 0.0111 0.0001 0.0005 0.0005 0.0001 0 0.0002 0.0002 0.0002 0.0001 0.0006 0.0001
Nucleus 0.0139 0.0061 0.005 0.0048 0.005 0.0181 0.0279 0.0028 0.002 0.0487 0.0006 0.0006 0.0014 0.0027 0.0017 0.0009 0.0006 0.0011
Peroxisomes 0.0107 0.0002 0.0058 0.0005 0.0249 0.0001 0.0221 0 0.0119 0.0033 0.0345 0.0007 0.0127 0.0036 0.0164 0.0033 0.0902 0.0004
Punctate Nuclear 0.0052 0.0015 0.0016 0.0005 0.0009 0.0023 0.0125 0.0003 0.0056 0.0025 0.0011 0.0005 0.0086 0.0029 0.0025 0.0006 0.0275 0.0008
Vacuole 0.0014 0.0016 0.0011 0.0014 0.0005 0.0016 0.005 0.0036 0.0008 0.0024 0.0002 0.0003 0.0006 0.0011 0.0007 0.0004 0.0016 0.0002
Vacuole Periphery 0.0006 0.0002 0.0004 0.0005 0.0002 0.0007 0.0032 0.0011 0.0007 0.0012 0.0001 0.0001 0.001 0.0002 0.0003 0.0002 0.0009 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.2236 70.127 37.5351 39.3983 12.5054 88.6862 110.4263 133.37 106.8842 133.2555
Translational Efficiency 2.539 2.1124 2.5427 1.8511 2.4246 2.4925 2.3241 1.8078 2.0304 1.8958

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1833 1623 1975 1692 2036 1613 2138 1753 3869 3236 4113 3445

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 799.73 693.70 908.18 930.30 834.65 789.71 982.20 960.68 818.11 741.56 946.66 945.76
Standard Deviation 133.19 97.09 157.95 152.64 147.14 93.06 145.45 142.88 141.78 106.53 156.02 148.53
Intensity Change Log 2 — -0.205201 0.183465 0.218183 — -0.079848 0.234845 0.202885 — -0.139826 0.209933 0.210391

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000107 0.000367 0.000258 0.001434 0.000139 0.000233 0.000240 0.000516 0.000124 0.000300 0.000249 0.000967
Bud Neck 0.009839 0.039836 0.003851 0.010773 0.003836 0.027413 0.002331 0.005580 0.006680 0.033643 0.003061 0.008130
Bud Site 0.004522 0.020174 0.007327 0.032942 0.003962 0.010818 0.006343 0.017939 0.004227 0.015511 0.006815 0.025308
Cell Periphery 0.000180 0.000145 0.000118 0.000351 0.000136 0.000211 0.000122 0.000164 0.000157 0.000178 0.000120 0.000256
Cytoplasm 0.590802* 0.432560* 0.430619* 0.505189* 0.673800* 0.451857* 0.513485* 0.565949* 0.634479* 0.442179* 0.473694* 0.536107*
Cytoplasmic Foci 0.169315 0.238932* 0.004825 0.011707 0.077985 0.219785* 0.007444 0.013143 0.121254 0.229388* 0.006186 0.012438
Eisosomes 0.000121 0.000121 0.000016 0.000037 0.000037 0.000176 0.000022 0.000030 0.000077 0.000149 0.000019 0.000034
Endoplasmic Reticulum 0.001413 0.000919 0.002665 0.002121 0.001799 0.000511 0.001893 0.003064 0.001616 0.000715 0.002264 0.002601
Endosome 0.007590 0.021940 0.000900 0.005310 0.002740 0.016118 0.001363 0.005590 0.005038 0.019038 0.001141 0.005452
Golgi 0.001511 0.004435 0.000069 0.002621 0.000578 0.002835 0.000322 0.002835 0.001020 0.003637 0.000200 0.002730
Lipid Particles 0.005924 0.008280 0.000558 0.000991 0.002168 0.010234 0.000672 0.000974 0.003947 0.009254 0.000617 0.000982
Mitochondria 0.002597 0.013384 0.000670 0.002491 0.001045 0.008562 0.000416 0.002862 0.001780 0.010981 0.000538 0.002680
Mitotic Spindle 0.000884 0.007311 0.003424 0.062429 0.001700 0.004976 0.005871 0.024594 0.001313 0.006147 0.004696 0.043176
None 0.008811 0.005265 0.007074 0.011566 0.005084 0.004901 0.007353 0.008610 0.006849 0.005084 0.007219 0.010062
Nuclear Periphery 0.000348 0.000454 0.001314 0.001593 0.000455 0.000457 0.001240 0.001417 0.000404 0.000455 0.001275 0.001503
Nuclear Periphery Foci 0.001031 0.000532 0.001259 0.001299 0.001349 0.000257 0.001207 0.001871 0.001199 0.000395 0.001232 0.001590
Nucleolus 0.000821 0.002966 0.000297 0.000923 0.000437 0.002454 0.000249 0.000242 0.000619 0.002711 0.000272 0.000577
Nucleus 0.151408* 0.116507 0.505046* 0.235473* 0.190847* 0.175127* 0.400523* 0.253351* 0.172163* 0.145726* 0.450714* 0.244570*
Peroxisomes 0.002990 0.017369 0.000117 0.002239 0.000775 0.011499 0.000132 0.000520 0.001825 0.014443 0.000125 0.001364
Vacuole 0.038955 0.066118 0.029465 0.105182 0.030863 0.049619 0.048160 0.088538 0.034697 0.057894 0.039183 0.096713
Vacuole Periphery 0.000831 0.002385 0.000128 0.003328 0.000264 0.001957 0.000613 0.002211 0.000532 0.002171 0.000380 0.002760

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.60 -6.62 -3.01 -1.49 -1.73 -1.63 -2.95 -5.53 -2.86 -2.29 -4.92 -6.19 -4.36 -2.34 -2.35
Bud Neck -12.75 4.56 1.51 14.09 -3.58 -12.82 1.39 -2.87 11.74 -4.24 -18.09 4.71 -0.04 18.32 -5.28
Bud Site -8.93 -2.10 -6.18 2.10 -4.09 -5.14 -1.14 -4.43 0.42 -3.09 -10.23 -2.34 -7.60 1.95 -5.14
Cell Periphery 2.64 4.78 0.49 -0.49 -0.91 -3.83 1.69 1.05 5.05 -0.80 -1.59 4.13 0.79 1.66 -1.11
Cytoplasm 16.65 24.67 22.11 3.92 -1.67 25.83 31.12 29.41 2.65 0.40 29.85 39.15 36.37 4.61 -0.81
Cytoplasmic Foci -9.80 36.12 35.49 41.71 -4.44 -24.30 26.77 25.14 40.13 -4.67 -23.36 43.18 41.86 57.82 -6.40
Eisosomes -0.01 17.64 13.39 16.56 -10.38 -17.13 4.54 -1.29 17.00 -7.68 -13.38 17.56 11.39 22.95 -12.84
Endoplasmic Reticulum 1.95 -7.41 -5.04 -11.81 3.81 6.23 -4.01 -3.74 -8.72 -1.31 5.55 -8.19 -5.94 -13.00 0.80
Endosome -10.92 14.35 10.37 15.69 -4.75 -12.10 7.85 0.16 11.52 -3.39 -16.31 16.12 7.73 19.29 -5.33
Golgi -7.34 9.67 2.19 7.49 -1.96 -7.31 2.44 -0.62 3.51 -1.45 -10.37 8.01 0.71 7.56 -2.28
Lipid Particles -2.29 10.43 9.48 7.73 -6.50 -9.05 6.48 4.55 10.39 -5.99 -7.83 12.12 10.45 12.76 -8.86
Mitochondria -7.59 3.00 1.90 9.35 -3.68 -7.91 1.83 -0.84 7.47 -2.82 -10.81 3.49 1.04 11.94 -4.27
Mitotic Spindle -4.39 -4.41 -8.88 -6.15 -7.29 -2.73 -3.48 -6.76 -4.69 -4.37 -5.09 -5.39 -11.14 -7.71 -8.41
None 2.79 2.36 0.28 -3.19 -2.94 0.08 -3.17 -3.37 -2.69 -0.84 2.43 0.76 -1.33 -4.16 -2.82
Nuclear Periphery -2.00 -22.85 -27.26 -23.75 -9.86 0.21 -25.72 -21.46 -21.41 -6.45 -1.38 -34.23 -34.15 -31.86 -11.51
Nuclear Periphery Foci 4.05 -2.87 -1.78 -6.82 1.78 7.08 -0.38 -2.14 -13.60 -2.34 8.03 -2.31 -2.68 -14.57 0.00
Nucleolus -6.53 5.07 0.12 5.87 -2.45 -4.62 3.41 2.72 4.95 -1.78 -7.68 6.06 0.70 7.62 -2.64
Nucleus 6.71 -52.43 -24.35 -29.77 25.17 2.81 -37.32 -20.34 -21.96 14.20 6.68 -62.59 -31.23 -36.19 27.63
Peroxisomes -11.46 7.95 4.69 13.34 -1.79 -10.02 2.77 2.19 11.03 -2.12 -15.26 7.75 5.02 17.29 -2.22
Vacuole -9.33 -4.32 -28.18 -22.37 -24.59 -6.45 -15.07 -26.03 -22.21 -14.81 -11.36 -14.29 -38.30 -31.35 -27.66
Vacuole Periphery -6.58 4.23 -0.34 4.45 -2.96 -5.00 -0.36 -1.44 2.57 -0.92 -8.01 1.84 -1.33 4.63 -2.34
Standard name
Human Ortholog
Description mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; TIS11 has a paralog, CTH1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (66%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tis11

Tis11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tis11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available