Standard name
Human Ortholog
Description Mitochondrial membrane protein; coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0.1 0.29 0.07 – – 0 0 0 0 0 0 0
Bud 0 0.06 0 0 0 0 0 0.05 0.05 0 0.05 0.05 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 – – 0.05 0 0 0 0 0 0
Cytoplasm 0 0.08 0 0 0 0.07 0 0 0 0 0.06 0 0.12 0.19 0.38 – – 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0 – – 0.12 0 0 0 0 0 0
Endosome 0 0 0.06 0 0.12 0 0 0 0 0 0 0 0 0 0.06 – – 0.26 0.08 0.05 0.08 0.1 0.08 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.91 0.92 0.88 0.87 0.84 0.9 0.9 0.87 0.93 0.96 0.94 0.96 0.44 0.2 0.2 – – 0.11 0.76 0.79 0.7 0.7 0.66 0.68
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0.11 0.09 0.14 0 0 0 0 0 0 0 0 0 0 – – 0.18 0.09 0 0.12 0.09 0.1 0.07
SpindlePole 0.08 0 0 0 0.07 0.05 0.08 0.08 0.05 0.05 0 0.06 0.17 0.18 0.09 – – 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.05 0 0 0.08 0.07 0 0 0.05 0 0 0 0.1 0.09 0.17 – – 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 12 0 0 0 0 0 0 0 0 0 0 15 63 18 – – 0 1 5 6 2 14 24
Bud 1 17 13 10 9 9 22 45 27 17 24 26 3 5 6 – – 1 0 0 0 1 3 1
Bud Neck 0 3 0 0 0 0 0 1 1 0 0 0 3 2 1 – – 0 0 0 3 4 4 14
Bud Site 2 2 0 0 0 9 14 19 10 12 10 9 0 0 1 – – 0 – – – – – –
Cell Periphery 2 6 5 10 4 1 2 3 1 3 1 5 8 10 9 – – 3 0 0 1 0 1 0
Cytoplasm 3 22 14 11 14 27 30 41 23 10 29 12 19 41 94 – – 13 0 2 1 1 3 1
Endoplasmic Reticulum 2 7 2 1 1 0 0 1 2 5 1 0 8 24 3 – – 8 0 1 2 2 1 6
Endosome 2 0 20 7 43 9 20 18 2 2 2 5 7 5 15 – – 17 10 12 29 37 51 51
Golgi 2 0 1 2 0 0 0 0 0 0 0 0 10 6 3 – – 0 3 2 6 12 25 16
Mitochondria 87 243 287 215 290 352 632 797 521 607 448 544 69 45 50 – – 7 98 215 241 267 417 446
Nucleus 0 1 0 0 2 10 25 34 6 7 10 4 4 5 3 – – 1 0 0 0 0 0 2
Nuclear Periphery 1 2 4 4 0 12 11 12 11 16 11 8 3 0 0 – – 0 0 2 3 1 5 5
Nucleolus 0 0 2 1 3 9 12 21 11 10 9 6 0 0 0 – – 0 0 0 1 3 3 3
Peroxisomes 2 0 35 22 49 12 30 36 8 10 5 10 3 3 10 – – 12 11 8 40 34 66 43
SpindlePole 8 4 9 8 24 20 59 73 26 29 18 34 26 40 22 – – 7 0 2 0 2 13 5
Vac/Vac Membrane 8 14 9 8 29 26 30 35 28 18 9 15 16 20 41 – – 10 0 9 2 4 10 25
Unique Cell Count 96 263 327 246 347 391 702 916 559 633 477 569 157 220 245 66 129 271 347 382 633 657
Labelled Cell Count 121 333 401 299 468 496 887 1136 677 746 577 678 194 269 276 79 129 271 347 382 633 657


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 3.8 4.4 4.6 4.4 3.9 3.5 3.8 3.5 3.2 3.5 3.5 6.8 6.6 6.7 3.6 4.5 5.2 6.5 6.8 6.9
Std Deviation (1e-4) 0.6 0.5 1.2 1.4 1.2 1.6 1.2 1.5 1.4 0.8 1.4 1.1 0.9 0.9 1.0 0.5 1.1 1.3 1.5 1.5 1.8
Intensity Change (Log2) – – – 0.05 0.01 -0.2 -0.33 -0.23 -0.32 -0.49 -0.33 -0.35 0.62 0.58 0.61 -0.31 0.02 0.23 0.56 0.62 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5342 2.1214 2.4165 2.1377 1.3611 2.1663 1.5278 1.524 1.2787 1.5081 0.8222 1.247 0.8656 1.1296 0.7832 0.8846 0.3764 0.7834
Actin 0.0313 0.0081 0.0196 0.0212 0.0143 0.0259 0.0917 0.005 0.0103 0.0123 0.0009 0.0057 0.0139 0.0168 0.0226 0.0329 0.0027 0.0116
Bud 0.0006 0.0009 0.0003 0.0002 0.0045 0.0005 0.0008 0.0004 0.0006 0.0009 0.0001 0.0001 0.0004 0.0008 0.0004 0.0005 0.0005 0.0003
Bud Neck 0.0028 0.0004 0.0005 0.0003 0.0004 0.0011 0.0008 0.0002 0.0012 0.0004 0.0001 0.0005 0.0004 0.0004 0.001 0.0008 0.0003 0.0019
Bud Periphery 0.0009 0.0013 0.0004 0.0007 0.0038 0.0015 0.0014 0.0008 0.001 0.0015 0.0004 0.0004 0.0009 0.001 0.0006 0.0007 0.0013 0.0007
Bud Site 0.0052 0.0076 0.0009 0.0003 0.002 0.0018 0.0033 0.0011 0.0089 0.0009 0.0002 0.0002 0.0011 0.0057 0.0049 0.0011 0.0006 0.0003
Cell Periphery 0.0004 0.0006 0.0002 0.0004 0.0003 0.0005 0.0004 0.0002 0.0003 0.0002 0.0003 0.0001 0.0003 0.0002 0.0015 0.0002 0.0002 0.0002
Cytoplasm 0.0132 0.0043 0.0023 0.0002 0.0008 0.0009 0.0011 0.0006 0.0009 0.0002 0.0003 0.0002 0.0024 0.0023 0.0017 0.001 0.0028 0.0004
Cytoplasmic Foci 0.0219 0.0201 0.0107 0.0088 0.0336 0.0182 0.0222 0.0199 0.0118 0.0052 0.0118 0.008 0.025 0.0285 0.0261 0.0337 0.0395 0.0073
Eisosomes 0.0004 0.0004 0.0003 0.0036 0.0003 0.0003 0.0011 0.0002 0.0016 0.0003 0.0002 0.0002 0.0005 0.0003 0.0012 0.0004 0.0001 0.0003
Endoplasmic Reticulum 0.0104 0.0014 0.0014 0.0015 0.0003 0.0007 0.0009 0.0002 0.0002 0 0.0002 0.0001 0.0016 0.0006 0.0005 0.0006 0.0002 0.0007
Endosome 0.0881 0.0681 0.045 0.0219 0.0184 0.0377 0.0497 0.0349 0.0282 0.0039 0.0477 0.0217 0.0669 0.0569 0.0344 0.0333 0.0355 0.0331
Golgi 0.0716 0.0606 0.0368 0.0313 0.0549 0.0584 0.0526 0.052 0.0249 0.0165 0.0537 0.0438 0.0807 0.0733 0.067 0.0689 0.0389 0.0676
Lipid Particles 0.0373 0.0197 0.0061 0.037 0.0293 0.0313 0.0412 0.0185 0.0074 0.0004 0.0276 0.0337 0.0659 0.0157 0.0133 0.025 0.0079 0.0156
Mitochondria 0.6275 0.7119 0.8422 0.8515 0.7822 0.7634 0.5973 0.7616 0.8426 0.9506 0.7849 0.8394 0.6565 0.7532 0.7369 0.7107 0.8107 0.8068
None 0.0032 0.0011 0.0003 0.0006 0.0023 0.0085 0.0019 0.0004 0.0113 0.0001 0.0002 0.0001 0.0084 0.0003 0.0029 0.0006 0.0014 0.0001
Nuclear Periphery 0.009 0.0004 0.0011 0.0011 0.0004 0.001 0.0108 0.0001 0.0001 0 0.0003 0.0001 0.0072 0.0002 0.0022 0.0012 0.0001 0.0017
Nucleolus 0.0008 0.0002 0.0002 0.0001 0.001 0.0003 0.0005 0.0002 0.0003 0 0.0002 0.0001 0.0008 0.0001 0.0004 0.0002 0.0001 0.0001
Nucleus 0.0033 0.0003 0.0003 0.0002 0.0004 0.0003 0.0014 0.0001 0.0002 0 0.0001 0.0001 0.001 0.0001 0.0018 0.0002 0.0001 0.0002
Peroxisomes 0.056 0.0831 0.0222 0.0137 0.0465 0.0421 0.11 0.0995 0.0452 0.0056 0.0651 0.0426 0.0523 0.0408 0.0769 0.0792 0.0526 0.0449
Punctate Nuclear 0.0058 0.0003 0.0002 0.0002 0.0019 0.0003 0.0029 0.0001 0.0002 0 0.0001 0.0001 0.0042 0.0001 0.0008 0.0065 0.0002 0.0001
Vacuole 0.005 0.0046 0.006 0.0022 0.001 0.0026 0.0035 0.0017 0.0015 0.0003 0.0024 0.0008 0.0042 0.0012 0.0014 0.0009 0.0014 0.0026
Vacuole Periphery 0.0052 0.0046 0.0029 0.0029 0.0014 0.0025 0.0044 0.0021 0.0011 0.0005 0.0031 0.002 0.0054 0.0017 0.0016 0.0014 0.0028 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.569 9.1898 4.925 8.851 6.2941 6.8496 8.7756 10.2781 7.3656 9.7771
Translational Efficiency 1.0083 0.5914 1.1952 0.5402 0.6854 0.6786 0.6032 0.5915 0.8825 0.6868

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
95 987 1891 1003 1760 280 436 1423 1855 1267 2327 2426

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 956.66 733.26 888.19 899.18 619.34 781.02 821.43 847.09 636.62 743.81 875.68 868.63
Standard Deviation 164.73 127.24 104.80 121.53 89.14 107.33 97.80 124.16 120.24 124.71 106.75 125.72
Intensity Change Log 2 — -0.383681 -0.107138 -0.089396 — 0.334628 0.407406 0.451784 — -0.057636 0.117408 0.148009

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006220 0.002739 0.003859 0.038418 0.000859 0.002291 0.015490 0.020616 0.001133 0.002640 0.006038 0.027976
Bud Neck 0.020439 0.008237 0.004465 0.005857 0.010677 0.019042 0.018943 0.017281 0.011176 0.010625 0.007177 0.012558
Bud Site 0.055443 0.018776 0.021400 0.084156 0.010722 0.017148 0.082366 0.058792 0.013012 0.018416 0.032823 0.069278
Cell Periphery 0.000958 0.000466 0.000168 0.000773 0.000640 0.000491 0.001033 0.000679 0.000657 0.000471 0.000330 0.000717
Cytoplasm 0.014910 0.009744 0.006050 0.020159 0.006291 0.008307 0.007575 0.023765 0.006733 0.009427 0.006335 0.022274
Cytoplasmic Foci 0.111846 0.082851 0.098018 0.092031 0.039902 0.094387 0.041320 0.083029 0.043587 0.085401 0.087395 0.086751
Eisosomes 0.000420 0.000127 0.000087 0.000267 0.000103 0.000161 0.000153 0.000184 0.000119 0.000135 0.000099 0.000218
Endoplasmic Reticulum 0.003898 0.000393 0.002161 0.002499 0.000368 0.000372 0.002301 0.001266 0.000549 0.000389 0.002187 0.001776
Endosome 0.197286 0.098605 0.136575 0.083363 0.077957 0.116474 0.089642 0.107684 0.084068 0.102553 0.127781 0.097629
Golgi 0.081024 0.156977* 0.134172* 0.142568* 0.087005 0.146564* 0.044222 0.117999* 0.086699 0.154676* 0.117319* 0.128157*
Lipid Particles 0.010066 0.005661 0.004362 0.000844 0.008758 0.007286 0.001421 0.014440 0.008825 0.006020 0.003811 0.008819
Mitochondria 0.166255* 0.484004* 0.372648* 0.289077* 0.693730* 0.397159* 0.455705* 0.350625* 0.666716* 0.464811* 0.388210* 0.325179*
Mitotic Spindle 0.035410 0.024025 0.080815 0.067541 0.003858 0.012691 0.049214 0.053584 0.005474 0.021520 0.074894 0.059355
None 0.000281 0.000552 0.000213 0.000665 0.001663 0.000191 0.000217 0.001633 0.001592 0.000472 0.000214 0.001233
Nuclear Periphery 0.002360 0.000062 0.001131 0.000087 0.000098 0.000067 0.000116 0.000409 0.000214 0.000063 0.000941 0.000276
Nuclear Periphery Foci 0.003897 0.000255 0.001456 0.000428 0.000196 0.000208 0.000417 0.000707 0.000386 0.000245 0.001261 0.000591
Nucleolus 0.003838 0.001028 0.001103 0.000096 0.001908 0.000381 0.000489 0.002022 0.002007 0.000885 0.000988 0.001226
Nucleus 0.017912 0.001355 0.004103 0.000886 0.001933 0.002315 0.001522 0.004989 0.002751 0.001567 0.003619 0.003293
Peroxisomes 0.068410 0.085159 0.049075 0.134101* 0.033437 0.152058* 0.160688* 0.081505 0.035228 0.099943 0.069987 0.103251*
Vacuole 0.159448 0.010876 0.053334 0.026653 0.008569 0.011644 0.020335 0.045174 0.016296 0.011046 0.047151 0.037517
Vacuole Periphery 0.039680 0.008108 0.024806 0.009530 0.011325 0.010762 0.006833 0.013618 0.012778 0.008695 0.021439 0.011928

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.66 1.11 -7.82 -9.88 -9.55 -3.43 -4.62 -8.22 -7.54 -1.24 -3.39 -6.55 -13.05 -12.13 -10.06
Bud Neck 2.75 3.72 3.33 1.43 -1.66 -1.44 -2.52 -2.73 0.28 0.46 0.27 2.60 -0.80 -1.01 -3.95
Bud Site 3.75 3.49 -2.51 -12.64 -12.52 -1.89 -9.82 -13.33 -9.01 2.97 -2.43 -8.67 -18.17 -15.41 -10.74
Cell Periphery 2.42 4.05 1.08 -2.17 -5.77 1.19 -1.79 -0.27 -1.55 1.69 1.70 3.73 -0.53 -2.49 -5.47
Cytoplasm 1.71 2.59 -0.85 -4.81 -7.71 -1.00 -1.11 -8.76 -5.81 -8.06 -1.71 0.12 -10.20 -7.53 -11.45
Cytoplasmic Foci 2.16 1.09 1.54 -1.71 1.40 -7.99 -0.35 -12.19 1.65 -9.27 -11.63 -13.97 -14.26 -0.24 0.05
Eisosomes 1.19 1.36 0.62 -3.27 -4.35 -2.69 -4.30 -6.49 -0.96 -2.30 -0.93 1.34 -4.28 -4.09 -6.52
Endoplasmic Reticulum 3.94 1.71 1.26 -4.33 -0.51 -0.17 -2.56 -5.10 -4.69 1.50 2.07 -6.43 -5.31 -6.07 1.48
Endosome 4.53 2.80 5.30 2.81 10.30 -3.71 -1.54 -5.58 0.93 -2.15 -3.53 -9.16 -2.92 1.21 7.18
Golgi -5.24 -3.80 -4.18 1.87 -1.14 -5.35 8.21 -5.14 2.64 -12.14 -10.99 -6.28 -8.11 4.47 -2.02
Lipid Particles 0.65 0.84 1.40 4.64 6.09 0.58 6.65 -2.61 -2.55 -6.55 1.98 4.26 -0.05 -1.92 -3.95
Mitochondria -10.67 -7.18 -4.13 12.56 6.39 13.31 11.96 28.75* 2.20 5.28 15.48 25.69 32.23 11.62 6.51
Mitotic Spindle 0.92 -3.66 -2.50 -7.92 2.18 -4.16 -7.54 -13.32 -9.61 -0.69 -7.46 -20.49 -17.54 -10.58 3.49
None -1.16 0.99 -0.72 -0.31 -0.95 2.97 2.94 0.04 -2.09 -2.06 2.31 2.95 0.51 -1.64 -2.26
Nuclear Periphery 1.28 0.65 1.27 -1.55 4.27 1.28 -0.92 -2.98 -3.25 -2.67 1.60 -3.40 -0.65 -3.45 3.22
Nuclear Periphery Foci 1.47 0.95 1.39 -1.33 4.09 -0.35 -2.92 -4.47 -4.03 -1.93 0.94 -4.16 -1.61 -3.51 3.34
Nucleolus 1.33 1.34 1.94 2.85 3.82 2.53 2.34 -0.15 -4.29 -3.84 1.63 1.62 1.31 -0.69 -0.66
Nucleus 2.00 1.62 2.02 0.27 3.34 -0.50 0.34 -3.33 -0.84 -3.55 1.10 -1.16 -0.95 -2.17 0.37
Peroxisomes -1.01 1.08 -3.61 -5.43 -10.93 -8.03 -10.78 -9.53 4.66 6.43 -11.20 -8.50 -14.51 -0.41 -6.33
Vacuole 6.21 4.34 5.40 -6.35 6.91 -1.58 -4.04 -12.74 -10.28 -6.37 2.79 -12.23 -9.39 -12.84 2.26
Vacuole Periphery 4.92 2.28 4.67 -1.04 9.22 0.22 3.43 -1.62 -0.95 -4.62 3.42 -6.37 0.86 -2.80 7.49
Standard name
Human Ortholog
Description Mitochondrial membrane protein; coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery
Localization
Cell Percentages mitochondrion (91%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Sls1

Sls1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sls1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available