Standard name | |
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Human Ortholog | |
Description | Component of CORVET membrane tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 2 | 2 | 2 | 3 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 2 |
Bud | 0 | 0 | 12 | 6 | 6 | 24 | 35 | 50 | 28 | 44 | 36 | 35 | 2 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 6 | 23 | 15 | 34 | 33 | 14 | 29 | 18 | 9 | 8 | 16 | 4 | 6 | 6 | 6 | 11 | 7 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 1 | 2 | 2 | 4 | 7 | 7 | 3 | 1 | 0 | 1 | 2 | 1 | 1 | 7 | 0 | 0 | 2 | 0 | 1 | 3 |
Endosome | 120 | 34 | 524 | 316 | 468 | 300 | 322 | 388 | 192 | 230 | 116 | 414 | 74 | 209 | 207 | 69 | 90 | 78 | 170 | 130 | 624 | 133 | 260 | 305 |
Golgi | 1 | 2 | 2 | 2 | 3 | 3 | 1 | 6 | 2 | 7 | 0 | 5 | 0 | 2 | 4 | 7 | 8 | 7 | 5 | 2 | 17 | 1 | 3 | 6 |
Mitochondria | 53 | 76 | 164 | 222 | 212 | 453 | 534 | 350 | 502 | 582 | 495 | 558 | 16 | 5 | 7 | 20 | 18 | 10 | 4 | 10 | 6 | 2 | 9 | 9 |
Nucleus | 0 | 4 | 0 | 0 | 7 | 2 | 13 | 7 | 2 | 4 | 1 | 4 | 2 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 1 |
Nuclear Periphery | 9 | 16 | 14 | 11 | 5 | 12 | 24 | 16 | 17 | 15 | 8 | 15 | 7 | 5 | 7 | 9 | 2 | 7 | 3 | 1 | 10 | 0 | 1 | 3 |
Nucleolus | 85 | 44 | 161 | 119 | 130 | 122 | 124 | 142 | 79 | 100 | 74 | 84 | 44 | 65 | 65 | 25 | 30 | 36 | 1 | 1 | 1 | 1 | 1 | 0 |
Peroxisomes | 2 | 0 | 111 | 38 | 21 | 61 | 97 | 117 | 55 | 65 | 36 | 135 | 10 | 21 | 11 | 1 | 2 | 0 | 4 | 1 | 16 | 2 | 6 | 5 |
SpindlePole | 19 | 4 | 153 | 88 | 117 | 99 | 151 | 210 | 59 | 94 | 56 | 133 | 26 | 69 | 74 | 16 | 21 | 18 | 8 | 4 | 27 | 9 | 9 | 4 |
Vac/Vac Membrane | 57 | 49 | 72 | 44 | 112 | 89 | 79 | 90 | 52 | 81 | 62 | 138 | 19 | 81 | 51 | 52 | 57 | 58 | 9 | 16 | 6 | 6 | 12 | 20 |
Unique Cell Count | 198 | 154 | 700 | 517 | 756 | 848 | 989 | 1007 | 755 | 917 | 690 | 1074 | 118 | 269 | 264 | 126 | 152 | 140 | 212 | 173 | 723 | 161 | 314 | 367 |
Labelled Cell Count | 348 | 237 | 1236 | 861 | 1116 | 1201 | 1400 | 1419 | 1015 | 1242 | 900 | 1546 | 204 | 466 | 436 | 211 | 241 | 232 | 212 | 173 | 723 | 161 | 314 | 367 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 6.7 | 7.1 | 6.8 | 6.4 | 5.3 | 5.4 | 6.2 | 5.8 | 6.0 | 5.8 | 6.0 | 8.3 | 8.0 | 8.1 | 9.2 | 8.7 | 9.1 | 7.0 | 6.9 | 7.4 |
Std Deviation (1e-4) | 0.9 | 1.2 | 1.2 | 1.4 | 1.5 | 1.5 | 1.0 | 1.4 | 1.3 | 1.1 | 1.1 | 1.1 | 9.2 | 0.9 | 1.1 | 1.9 | 1.7 | 1.7 | 0.9 | 1.1 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.14 | -0.42 | -0.38 | -0.2 | -0.28 | -0.25 | -0.29 | -0.24 | 0.23 | 0.17 | 0.19 | 0.38 | 0.3 | 0.36 | -0.02 | -0.04 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.8 | -1.6 | 1.4 | 2.2 | 3.5 | 2.3 | 3.4 | 3.6 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.4 | 1.2 | 0.6 | -2.6 | -0.5 | -1.0 | -3.3 | -2.7 | -2.5 | 0 | -0.9 | -0.8 | 0 | 2.3 | 1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -5.1 | -5.3 | -15.5 | -17.1 | -14.8 | -18.8 | -19.9 | -21.7 | -15.0 | -2.8 | 0.9 | 1.1 | -4.6 | -3.9 | -4.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 7.2 | 2.0 | 12.0 | 12.6 | 5.0 | 16.5 | 16.0 | 18.0 | 12.0 | -2.4 | -7.9 | -7.5 | -1.9 | -3.2 | -4.3 |
Nucleus | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.2 | -2.2 | -0.9 | 0.6 | -0.6 | 0.3 | -0.5 | -1.3 | -1.0 | 0 | -0.1 | 0.6 | 0 | 0 | 0 |
Nucleolus | 0 | -2.8 | -4.4 | -5.7 | -4.7 | -6.4 | -6.5 | -6.1 | -9.1 | 3.3 | 0.4 | 0.5 | -0.8 | -0.9 | 0.7 |
Peroxisomes | -4.5 | -8.7 | -5.4 | -3.7 | -2.5 | -5.1 | -5.6 | -6.4 | -2.0 | -2.1 | -3.3 | -4.9 | -4.5 | -4.8 | -5.1 |
SpindlePole | -2.1 | -3.1 | -5.4 | -3.5 | -0.5 | -7.6 | -6.4 | -7.2 | -5.3 | 0 | 1.3 | 2.0 | -2.3 | -2.2 | -2.4 |
Vacuole | -1.0 | 2.6 | 0.1 | -1.6 | -0.9 | -2.3 | -1.0 | -0.8 | 1.6 | 1.9 | 7.6 | 3.7 | 9.0 | 8.5 | 9.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0628 | 2.5346 | 2.341 | 1.7931 | 1.8385 | 2.1899 | 3.5594 | 4.1402 | 3.9164 | 3.8148 | 3.8673 | 4.0629 | 2.12 | 2.9474 | 2.725 | 2.6916 | 2.7473 | 2.4627 |
Actin | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0025 | 0 | 0.0027 | 0.005 | 0.0006 | 0.0016 | 0.0131 | 0.0001 | 0.0016 | 0.005 | 0.0009 | 0.0006 |
Bud | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0.0005 | 0.0002 | 0 |
Bud Neck | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0007 | 0.0006 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0013 | 0.001 | 0.0003 | 0.0008 | 0.0003 | 0.0002 |
Bud Periphery | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0.0001 |
Bud Site | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0005 | 0.0003 | 0.0011 | 0.0003 | 0.0002 | 0.0067 | 0.0003 | 0.0007 | 0.0019 | 0.0003 | 0.0001 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0007 | 0.0008 | 0.0006 | 0.0006 | 0.0005 | 0.0004 | 0.0059 | 0.0004 | 0.0005 | 0.0013 | 0.0008 | 0.0021 | 0.0055 | 0.0005 | 0.0013 | 0.0007 | 0.0005 | 0.0003 |
Cytoplasmic Foci | 0.1339 | 0.1346 | 0.1343 | 0.1854 | 0.1526 | 0.0822 | 0.1204 | 0.1528 | 0.1369 | 0.1445 | 0.1249 | 0.0983 | 0.1363 | 0.134 | 0.1351 | 0.1475 | 0.1286 | 0.073 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0.0016 | 0.001 | 0.0003 | 0.0016 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 |
Endosome | 0.4171 | 0.3472 | 0.4032 | 0.5178 | 0.4997 | 0.4102 | 0.4014 | 0.3353 | 0.3739 | 0.5082 | 0.4604 | 0.3816 | 0.3916 | 0.3429 | 0.3973 | 0.5926 | 0.5155 | 0.2951 |
Golgi | 0.056 | 0.0721 | 0.0972 | 0.1236 | 0.0957 | 0.095 | 0.0709 | 0.0715 | 0.0893 | 0.0808 | 0.0913 | 0.0901 | 0.0491 | 0.0638 | 0.0907 | 0.0798 | 0.116 | 0.1032 |
Lipid Particles | 0.2997 | 0.3392 | 0.2583 | 0.07 | 0.1959 | 0.3138 | 0.3028 | 0.3308 | 0.2486 | 0.1701 | 0.2462 | 0.3444 | 0.2729 | 0.3597 | 0.2464 | 0.089 | 0.1461 | 0.4477 |
Mitochondria | 0.0024 | 0.0096 | 0.0133 | 0.0011 | 0.0029 | 0.0072 | 0.0063 | 0.0016 | 0.0148 | 0.0019 | 0.0113 | 0.0034 | 0.022 | 0.0174 | 0.0101 | 0.0025 | 0.035 | 0.0064 |
None | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0018 | 0.0001 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0038 | 0.0001 | 0.0014 | 0.0002 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0051 | 0.0005 | 0.0007 | 0.0005 | 0.0001 | 0.0005 | 0.0009 | 0.0002 | 0.0002 | 0.0013 | 0.002 | 0.0007 | 0.0014 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0003 |
Nucleolus | 0.0048 | 0.0031 | 0.0021 | 0.0003 | 0.0023 | 0.0037 | 0.0009 | 0.0087 | 0.0017 | 0.0002 | 0.0031 | 0.0005 | 0.0034 | 0.0052 | 0.0008 | 0.0006 | 0.0002 | 0.0038 |
Nucleus | 0.0013 | 0.0008 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0007 | 0.0007 | 0.0002 | 0.0002 | 0.0005 | 0.003 | 0.0009 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 |
Peroxisomes | 0.0442 | 0.0393 | 0.0484 | 0.0491 | 0.0347 | 0.0478 | 0.0599 | 0.0711 | 0.1111 | 0.0706 | 0.0361 | 0.054 | 0.0482 | 0.0334 | 0.069 | 0.0518 | 0.0448 | 0.0489 |
Punctate Nuclear | 0.0034 | 0.0042 | 0.0007 | 0.0005 | 0.0035 | 0.0005 | 0.0046 | 0.0079 | 0.0008 | 0.0023 | 0.0019 | 0.0043 | 0.0082 | 0.0007 | 0.0008 | 0.0012 | 0.0004 | 0.0023 |
Vacuole | 0.0228 | 0.035 | 0.0286 | 0.0316 | 0.0076 | 0.0238 | 0.0131 | 0.0143 | 0.0137 | 0.0073 | 0.0117 | 0.0084 | 0.0238 | 0.0223 | 0.0329 | 0.0174 | 0.0072 | 0.01 |
Vacuole Periphery | 0.007 | 0.0116 | 0.011 | 0.0188 | 0.0033 | 0.0134 | 0.0058 | 0.0029 | 0.0039 | 0.0025 | 0.007 | 0.0065 | 0.0094 | 0.0176 | 0.0102 | 0.0071 | 0.0031 | 0.0075 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.2078 | 10.3754 | 9.5573 | 16.8275 | 18.421 | 11.1999 | 14.4697 | 15.2595 | 15.5483 | 16.937 |
Translational Efficiency | 0.8351 | 1.0686 | 1.261 | 0.6825 | 0.5729 | 1.0871 | 0.9331 | 0.7442 | 0.9051 | 0.7598 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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