Standard name | |
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Human Ortholog | |
Description | Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 |
Bud | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 4 | 3 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 0 | 4 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 3 |
Bud Site | 1 | 0 | 0 | 1 | 1 | 9 | 8 | 7 | 8 | 13 | 14 | 9 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 8 | 5 | 13 | 14 | 13 | 19 | 16 | 20 | 9 | 8 | 11 | 5 | 19 | 14 | 25 | 8 | 10 | 4 | 3 | 0 | 0 | 0 | 2 | 5 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 5 | 2 | 3 | 0 | 0 | 0 | 1 | 1 | 2 |
Endosome | 3 | 4 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 5 | 0 | 3 | 4 | 2 | 1 | 3 | 6 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 2 | 1 | 1 | 2 | 2 |
Mitochondria | 24 | 25 | 2 | 12 | 2 | 43 | 54 | 39 | 71 | 80 | 87 | 42 | 3 | 1 | 2 | 7 | 1 | 3 | 1 | 2 | 1 | 0 | 3 | 3 |
Nucleus | 186 | 205 | 202 | 157 | 200 | 300 | 328 | 366 | 183 | 247 | 177 | 265 | 166 | 169 | 159 | 136 | 118 | 119 | 174 | 197 | 176 | 42 | 92 | 91 |
Nuclear Periphery | 2 | 1 | 0 | 1 | 0 | 4 | 0 | 1 | 2 | 2 | 4 | 2 | 2 | 1 | 1 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 36 | 26 | 2 | 7 | 6 | 21 | 44 | 43 | 56 | 80 | 55 | 56 | 30 | 27 | 34 | 19 | 11 | 11 | 26 | 12 | 24 | 9 | 34 | 29 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 1 | 3 | 2 | 3 | 5 | 5 | 12 | 1 | 1 | 1 | 3 | 1 | 1 | 1 | 0 | 2 | 4 | 8 | 2 | 9 | 1 | 3 | 5 |
Vac/Vac Membrane | 9 | 11 | 8 | 3 | 2 | 2 | 2 | 6 | 0 | 0 | 2 | 2 | 0 | 2 | 2 | 12 | 13 | 14 | 10 | 9 | 9 | 5 | 24 | 20 |
Unique Cell Count | 217 | 232 | 227 | 178 | 220 | 337 | 377 | 414 | 230 | 304 | 237 | 308 | 189 | 201 | 198 | 169 | 156 | 145 | 236 | 241 | 235 | 67 | 180 | 190 |
Labelled Cell Count | 271 | 279 | 234 | 197 | 228 | 403 | 460 | 499 | 334 | 434 | 357 | 394 | 222 | 217 | 227 | 193 | 165 | 160 | 236 | 241 | 235 | 67 | 180 | 190 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 5.9 | 6.0 | 5.6 | 6.3 | 5.3 | 5.1 | 5.6 | 4.8 | 4.7 | 4.4 | 4.9 | 6.4 | 6.7 | 6.5 | 9.1 | 9.5 | 8.9 | 5.8 | 5.9 | 7.4 |
Std Deviation (1e-4) | 1.1 | 1.1 | 1.0 | 1.1 | 1.3 | 1.7 | 1.1 | 1.4 | 1.2 | 1.2 | 1.2 | 0.9 | 2.0 | 1.8 | 1.6 | 2.1 | 2.4 | 1.9 | 0.9 | 1.6 | 3.9 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.08 | -0.18 | -0.22 | -0.1 | -0.31 | -0.35 | -0.43 | -0.28 | 0.1 | 0.16 | 0.12 | 0.6 | 0.67 | 0.57 | -0.04 | -0.03 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.9 | 0.1 | 0 | -0.8 | -0.5 | -0.9 | -1.8 | -0.5 | -2.6 | 1.6 | 0.5 | 2.5 | -0.4 | 0.3 | -1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.2 | 0 | 5.1 | 5.5 | 4.2 | 0 | 0 | 0 | 5.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.2 | 0.7 | 0 | -0.7 | -0.2 | -2.8 | -2.4 | -4.0 | -1.0 | -0.4 | -1.5 | -2.5 | -2.4 | -3.5 | -1.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 3.2 | 4.8 | 4.5 | 0 | 0 | 0 | 6.4 | 5.7 | 5.2 | 6.0 | 4.6 | 3.3 | 3.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 2.0 | 2.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4284 | 4.3153 | 4.2054 | 3.529 | 4.338 | 3.911 | 2.6457 | 2.6457 | 2.4154 | 2.0137 | 1.7227 | 2.0909 | 2.3882 | 2.3443 | 2.0974 | 2.4727 | 3.6322 | 1.7878 |
Actin | 0.0831 | 0.0001 | 0.002 | 0.0079 | 0.0017 | 0.0077 | 0.0216 | 0.0001 | 0.0034 | 0.0044 | 0.0001 | 0.0023 | 0.0286 | 0 | 0.0125 | 0.0065 | 0.0243 | 0 |
Bud | 0.0012 | 0.0003 | 0.0002 | 0.0031 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0007 | 0.0022 | 0.0001 | 0.0002 | 0.0009 | 0 | 0.0007 | 0.0002 | 0.0037 | 0 |
Bud Neck | 0.0077 | 0.0003 | 0.0013 | 0.0027 | 0.0018 | 0.0019 | 0.0008 | 0.0001 | 0.0004 | 0.0009 | 0.001 | 0.0011 | 0.0015 | 0.0001 | 0.0004 | 0.0009 | 0.0016 | 0.0012 |
Bud Periphery | 0.0023 | 0.0008 | 0.0003 | 0.0058 | 0.0001 | 0.0005 | 0.0005 | 0.0001 | 0.0007 | 0.0054 | 0.0001 | 0.0006 | 0.0018 | 0 | 0.0021 | 0.0003 | 0.0037 | 0.0001 |
Bud Site | 0.0174 | 0.0062 | 0.0013 | 0.0384 | 0.0003 | 0.0004 | 0.0011 | 0.0001 | 0.005 | 0.0015 | 0.0005 | 0.0002 | 0.0038 | 0.0001 | 0.0005 | 0.0007 | 0.004 | 0.0001 |
Cell Periphery | 0.0006 | 0.001 | 0.0002 | 0.0016 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0038 | 0.0002 | 0.001 | 0.0024 | 0.0057 | 0.0001 | 0.0038 | 0.0001 | 0.0035 | 0.0023 | 0.0016 | 0.0006 | 0.0013 | 0.0001 | 0.0001 | 0.0004 | 0.0052 | 0.0009 |
Cytoplasmic Foci | 0.0227 | 0 | 0.0027 | 0.0101 | 0.0007 | 0.0004 | 0.0226 | 0 | 0.0115 | 0.0006 | 0.033 | 0.0008 | 0.0234 | 0 | 0.0002 | 0.0094 | 0.0159 | 0.0003 |
Eisosomes | 0.0006 | 0 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0047 | 0.0001 | 0.0005 | 0.0006 | 0.0032 | 0.0008 | 0.0047 | 0 | 0.0009 | 0.0017 | 0.0006 | 0.0015 | 0.0027 | 0 | 0.0003 | 0.0001 | 0.0018 | 0 |
Endosome | 0.0346 | 0.0001 | 0.002 | 0.0066 | 0.0122 | 0.0015 | 0.0584 | 0 | 0.0081 | 0.0059 | 0.0322 | 0.0041 | 0.0227 | 0 | 0.0012 | 0.0058 | 0.0265 | 0.0001 |
Golgi | 0.0169 | 0 | 0.001 | 0.0052 | 0.0005 | 0.0008 | 0.0152 | 0 | 0.0038 | 0.0012 | 0.0034 | 0.0011 | 0.0112 | 0 | 0.0008 | 0.0109 | 0.0107 | 0 |
Lipid Particles | 0.0571 | 0.0001 | 0.0009 | 0.0188 | 0.0008 | 0.0029 | 0.0286 | 0 | 0.006 | 0.0007 | 0.0144 | 0.0012 | 0.0305 | 0 | 0.0006 | 0.05 | 0.0022 | 0 |
Mitochondria | 0.0125 | 0.0006 | 0.0035 | 0.0043 | 0.0005 | 0.0029 | 0.025 | 0.0018 | 0.0027 | 0.0141 | 0.0004 | 0.0066 | 0.0125 | 0.0002 | 0.0026 | 0.0036 | 0.0194 | 0.0001 |
None | 0.006 | 0.0001 | 0.0096 | 0.0013 | 0.0021 | 0.0004 | 0.0033 | 0 | 0.0006 | 0.0009 | 0.0001 | 0.0002 | 0.0012 | 0 | 0.0001 | 0.0002 | 0.0025 | 0.0002 |
Nuclear Periphery | 0.0143 | 0.0079 | 0.0021 | 0.003 | 0.05 | 0.0014 | 0.0192 | 0.0076 | 0.009 | 0.0257 | 0.0034 | 0.0025 | 0.0038 | 0.0075 | 0.0013 | 0.0029 | 0.0039 | 0.0007 |
Nucleolus | 0.0246 | 0.0299 | 0.0288 | 0.009 | 0.073 | 0.1279 | 0.0366 | 0.0348 | 0.0329 | 0.039 | 0.1071 | 0.1031 | 0.0258 | 0.0296 | 0.0195 | 0.0348 | 0.0314 | 0.0837 |
Nucleus | 0.6257 | 0.948 | 0.9324 | 0.8627 | 0.7702 | 0.8459 | 0.736 | 0.9538 | 0.9013 | 0.8839 | 0.7922 | 0.8686 | 0.772 | 0.9619 | 0.9529 | 0.8452 | 0.7142 | 0.8986 |
Peroxisomes | 0.0372 | 0 | 0.0053 | 0.0067 | 0.0001 | 0.0013 | 0.0062 | 0 | 0.0042 | 0.0007 | 0.0006 | 0.0011 | 0.0459 | 0 | 0.0004 | 0.0232 | 0.0075 | 0 |
Punctate Nuclear | 0.0139 | 0.0019 | 0.0039 | 0.0063 | 0.0749 | 0.0016 | 0.0059 | 0.001 | 0.0034 | 0.003 | 0.0076 | 0.0029 | 0.004 | 0.0004 | 0.0029 | 0.0038 | 0.1194 | 0.0138 |
Vacuole | 0.0104 | 0.0015 | 0.0007 | 0.0028 | 0.0011 | 0.0007 | 0.0065 | 0.0001 | 0.001 | 0.0026 | 0.0013 | 0.0006 | 0.0041 | 0 | 0.0004 | 0.0006 | 0.0011 | 0.0001 |
Vacuole Periphery | 0.0029 | 0.001 | 0.0002 | 0.0004 | 0.0008 | 0.0003 | 0.0035 | 0.0002 | 0.0007 | 0.0027 | 0.0002 | 0.0006 | 0.0013 | 0.0001 | 0.0003 | 0.0002 | 0.0008 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.5538 | 15.9489 | 13.0486 | 15.8848 | 18.0902 | 15.5452 | 18.7102 | 18.5584 | 17.2624 | 22.5457 |
Translational Efficiency | 1.8648 | 1.4965 | 1.0946 | 0.9255 | 1.1453 | 1.4961 | 1.0891 | 0.7697 | 0.9273 | 0.962 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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