Standard name | |
---|---|
Human Ortholog | |
Description | Epsin-like protein required for endocytosis and actin patch assembly; functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus; ENT2 has a paralog, ENT1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 75 | 108 | 59 | 30 | 39 | 55 | 64 | 68 | 67 | 49 | 57 | 51 | – | 5 | 3 | 55 | 43 | 37 | 229 | 315 | 129 | 17 | 33 | 43 |
Bud | 25 | 26 | 6 | 7 | 13 | 16 | 25 | 22 | 32 | 15 | 26 | 16 | – | 1 | 1 | 11 | 11 | 11 | 8 | 9 | 6 | 0 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 25 | 42 | 21 | 0 | 4 | 4 |
Bud Site | 4 | 2 | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 3 | 0 | 1 | – | 0 | 0 | 0 | 2 | 1 | – | – | – | – | – | – |
Cell Periphery | 29 | 109 | 30 | 23 | 19 | 26 | 31 | 32 | 16 | 18 | 23 | 34 | – | 3 | 10 | 15 | 11 | 7 | 27 | 55 | 14 | 3 | 2 | 3 |
Cytoplasm | 207 | 243 | 104 | 78 | 122 | 105 | 115 | 118 | 94 | 87 | 88 | 91 | – | 18 | 53 | 50 | 46 | 46 | 65 | 72 | 34 | 2 | 3 | 6 |
Endoplasmic Reticulum | 52 | 149 | 53 | 41 | 34 | 56 | 61 | 66 | 64 | 46 | 62 | 42 | – | 6 | 18 | 6 | 3 | 7 | 25 | 44 | 15 | 2 | 2 | 6 |
Endosome | 14 | 0 | 0 | 0 | 1 | 7 | 13 | 10 | 13 | 5 | 5 | 3 | – | 0 | 3 | 10 | 6 | 21 | 10 | 8 | 13 | 1 | 3 | 3 |
Golgi | 1 | 1 | 2 | 0 | 3 | 3 | 4 | 9 | 7 | 5 | 9 | 11 | – | 0 | 0 | 19 | 22 | 11 | 4 | 6 | 1 | 0 | 0 | 1 |
Mitochondria | 4 | 1 | 0 | 2 | 0 | 12 | 11 | 5 | 51 | 25 | 69 | 50 | – | 0 | 0 | 3 | 2 | 4 | 8 | 6 | 8 | 0 | 1 | 2 |
Nucleus | 3 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 3 | 2 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 1 | 0 | 3 | – | 0 | 0 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 1 | 0 | 0 | 0 | 3 | 0 | 3 | 2 | 4 | 1 | 2 | – | 0 | 0 | 1 | 6 | 8 | 1 | 0 | 3 | 0 | 0 | 4 |
SpindlePole | 2 | 4 | 4 | 0 | 11 | 13 | 20 | 29 | 23 | 7 | 3 | 7 | – | 1 | 0 | 37 | 42 | 41 | 3 | 0 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 5 | 8 | 3 | 0 | 7 | 17 | 27 | 21 | 42 | 34 | 24 | 30 | – | 0 | 0 | 12 | 16 | 12 | 11 | 9 | 3 | 0 | 1 | 0 |
Unique Cell Count | 345 | 498 | 208 | 148 | 207 | 245 | 295 | 294 | 319 | 229 | 271 | 248 | 28 | 72 | 169 | 159 | 152 | 427 | 577 | 256 | 32 | 63 | 87 | |
Labelled Cell Count | 424 | 653 | 262 | 182 | 249 | 316 | 377 | 387 | 417 | 302 | 368 | 342 | 34 | 88 | 220 | 215 | 208 | 427 | 577 | 256 | 32 | 63 | 87 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.4 | 11.1 | 9.6 | 9.2 | 10.8 | 10.0 | 10.0 | 10.4 | 8.8 | 9.4 | 8.8 | 9.6 | – | 11.6 | 11.3 | 16.1 | 16.3 | 14.9 | – | – | 10.5 |
Std Deviation (1e-4) | 1.3 | 1.7 | 1.2 | 1.3 | 1.6 | 1.8 | 1.7 | 1.9 | 1.9 | 1.8 | 2.0 | 2.4 | – | 1.9 | 1.5 | 3.9 | 3.8 | 3.3 | – | – | 1.8 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.17 | 0.06 | 0.06 | 0.12 | -0.13 | -0.03 | -0.12 | 0.01 | – | 0.28 | 0.24 | 0.75 | 0.76 | 0.64 | – | – | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.7 | -2.3 | -1.4 | -1.7 | -1.3 | -1.9 | -1.7 | -1.9 | -1.9 | – | -1.2 | -4.3 | 0.9 | -0.3 | -0.9 |
Bud | 0.9 | 1.7 | 1.8 | 2.6 | 2.2 | 3.1 | 1.8 | 2.9 | 1.8 | – | 0 | 0 | 1.7 | 1.8 | 1.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.3 | -1.7 | -1.2 | -1.3 | -1.2 | -3.7 | -2.2 | -2.1 | -0.2 | – | 0 | -0.1 | -1.7 | -2.3 | -3.0 |
Cytoplasm | 0.5 | 1.8 | -1.5 | -2.5 | -2.2 | -4.8 | -2.5 | -3.9 | -2.9 | – | 1.4 | 3.5 | -4.0 | -4.1 | -3.8 |
Endoplasmic Reticulum | 0.5 | -2.3 | -0.7 | -1.3 | -0.8 | -1.5 | -1.3 | -0.7 | -2.2 | – | -0.5 | -0.1 | -5.8 | -6.2 | -5.2 |
Endosome | 0 | 0 | 0 | 3.1 | 0 | 2.9 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 5.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | – | 0 | 0 | 4.3 | 4.9 | 3.2 |
Mitochondria | 0 | 0 | 3.2 | 0 | 0 | 6.1 | 4.9 | 7.9 | 6.9 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1.9 | 1.9 | 2.5 | 3.5 | 2.7 | 0.8 | 0 | 0.6 | – | 0 | 0 | 6.2 | 7.0 | 7.1 |
Vacuole | 0 | 0 | 2.8 | 3.6 | 2.9 | 4.7 | 5.0 | 3.5 | 4.4 | – | 0 | 0 | 2.8 | 3.7 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.8452 | 5.9226 | 5.5315 | 4.3859 | 4.1501 | 5.5871 | 6.1241 | 7.0026 | 6.4127 | 5.9262 | 5.5532 | 6.6143 | 6.2364 | 6.853 | 5.8683 | 6.1245 | 5.6411 | 6.7494 |
Actin | 0.3517 | 0.3351 | 0.4803 | 0.3315 | 0.4003 | 0.4441 | 0.4607 | 0.3547 | 0.4527 | 0.5082 | 0.3655 | 0.4151 | 0.5902 | 0.4137 | 0.5677 | 0.5635 | 0.5 | 0.4559 |
Bud | 0.0041 | 0.0022 | 0.0094 | 0.0018 | 0.0032 | 0.0101 | 0.0021 | 0.0055 | 0.0043 | 0.0019 | 0.0069 | 0.0032 | 0.0159 | 0.0053 | 0.0124 | 0.001 | 0.005 | 0.0022 |
Bud Neck | 0.0245 | 0.0087 | 0.0276 | 0.0123 | 0.0312 | 0.232 | 0.0218 | 0.0126 | 0.047 | 0.0127 | 0.0144 | 0.2233 | 0.0279 | 0.0163 | 0.0449 | 0.0147 | 0.0047 | 0.2429 |
Bud Periphery | 0.0077 | 0.0025 | 0.0234 | 0.0035 | 0.0109 | 0.0228 | 0.004 | 0.0056 | 0.0128 | 0.0065 | 0.0194 | 0.0137 | 0.0107 | 0.0035 | 0.0158 | 0.0019 | 0.0074 | 0.0091 |
Bud Site | 0.0571 | 0.1233 | 0.0393 | 0.0025 | 0.0087 | 0.0126 | 0.0321 | 0.147 | 0.0604 | 0.003 | 0.008 | 0.0063 | 0.0358 | 0.1278 | 0.0577 | 0.0019 | 0.0042 | 0.0026 |
Cell Periphery | 0.0204 | 0.0188 | 0.0095 | 0.003 | 0.0113 | 0.019 | 0.0184 | 0.0098 | 0.012 | 0.0063 | 0.0119 | 0.0127 | 0.0203 | 0.0122 | 0.0115 | 0.0037 | 0.0021 | 0.0147 |
Cytoplasm | 0.2607 | 0.443 | 0.3181 | 0.1902 | 0.4158 | 0.1665 | 0.3 | 0.3818 | 0.3008 | 0.2817 | 0.2353 | 0.2464 | 0.1915 | 0.3828 | 0.2161 | 0.3496 | 0.1595 | 0.2155 |
Cytoplasmic Foci | 0.053 | 0.0177 | 0.019 | 0.1032 | 0.0265 | 0.0237 | 0.0386 | 0.031 | 0.0271 | 0.0321 | 0.0761 | 0.0192 | 0.0215 | 0.0099 | 0.0115 | 0.0153 | 0.1285 | 0.0119 |
Eisosomes | 0.0031 | 0.0016 | 0.0012 | 0.0006 | 0.0018 | 0.0011 | 0.0052 | 0.001 | 0.0058 | 0.0015 | 0.0041 | 0.0025 | 0.0076 | 0.0011 | 0.002 | 0.0067 | 0.0006 | 0.002 |
Endoplasmic Reticulum | 0.0247 | 0.0099 | 0.0087 | 0.0292 | 0.0301 | 0.0142 | 0.0154 | 0.0051 | 0.0122 | 0.0191 | 0.0198 | 0.0079 | 0.0066 | 0.0138 | 0.0054 | 0.006 | 0.0029 | 0.0095 |
Endosome | 0.0422 | 0.0063 | 0.0117 | 0.0987 | 0.0213 | 0.0148 | 0.022 | 0.0054 | 0.014 | 0.0361 | 0.0581 | 0.0108 | 0.0195 | 0.001 | 0.0093 | 0.0026 | 0.0369 | 0.0059 |
Golgi | 0.0322 | 0.0174 | 0.0186 | 0.0672 | 0.0112 | 0.0209 | 0.0323 | 0.0163 | 0.0302 | 0.0341 | 0.0669 | 0.0135 | 0.0144 | 0.0051 | 0.0132 | 0.0239 | 0.0461 | 0.0081 |
Lipid Particles | 0.025 | 0.0029 | 0.0045 | 0.024 | 0.0062 | 0.0062 | 0.0108 | 0.0012 | 0.0032 | 0.0034 | 0.036 | 0.0063 | 0.0118 | 0.0011 | 0.0031 | 0.0028 | 0.0147 | 0.006 |
Mitochondria | 0.0222 | 0.0006 | 0.0023 | 0.0235 | 0.0021 | 0.0014 | 0.0063 | 0.0095 | 0.0054 | 0.0212 | 0.0237 | 0.0027 | 0.0027 | 0.0006 | 0.0071 | 0.0007 | 0.0083 | 0.0017 |
None | 0.0194 | 0.0057 | 0.012 | 0.0024 | 0.0047 | 0.0022 | 0.0115 | 0.0014 | 0.0021 | 0.003 | 0.0041 | 0.0019 | 0.0046 | 0.0024 | 0.0104 | 0.001 | 0.0219 | 0.0052 |
Nuclear Periphery | 0.0135 | 0.0007 | 0.0008 | 0.0108 | 0.0013 | 0.001 | 0.0016 | 0.0005 | 0.0017 | 0.0045 | 0.0041 | 0.0044 | 0.0036 | 0.0004 | 0.0015 | 0.0003 | 0.0019 | 0.0012 |
Nucleolus | 0.0054 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0004 |
Nucleus | 0.0031 | 0.0006 | 0.0007 | 0.0013 | 0.0013 | 0.0008 | 0.0014 | 0.0021 | 0.0009 | 0.0009 | 0.0013 | 0.0008 | 0.0015 | 0.0005 | 0.0023 | 0.0003 | 0.0011 | 0.0008 |
Peroxisomes | 0.0146 | 0.0019 | 0.0105 | 0.0785 | 0.0076 | 0.0036 | 0.01 | 0.0083 | 0.0053 | 0.0044 | 0.0281 | 0.0072 | 0.0075 | 0.0016 | 0.0045 | 0.0034 | 0.0165 | 0.0026 |
Punctate Nuclear | 0.009 | 0.0004 | 0.0013 | 0.0018 | 0.0027 | 0.0014 | 0.0028 | 0.0006 | 0.0007 | 0.0005 | 0.0126 | 0.0009 | 0.0032 | 0.0004 | 0.0016 | 0.0005 | 0.0364 | 0.0011 |
Vacuole | 0.0048 | 0.0005 | 0.0007 | 0.0051 | 0.0013 | 0.0009 | 0.0018 | 0.0004 | 0.0008 | 0.007 | 0.0023 | 0.0005 | 0.0022 | 0.0002 | 0.0012 | 0.0001 | 0.0007 | 0.0006 |
Vacuole Periphery | 0.0017 | 0.0001 | 0.0001 | 0.0088 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0118 | 0.0009 | 0.0004 | 0.0007 | 0 | 0.0004 | 0 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.8498 | 29.9875 | 29.4045 | 43.6995 | 38.0565 | 35.9216 | 45.6159 | 55.9112 | 51.7894 | 47.8344 |
Translational Efficiency | 1.65 | 1.4711 | 1.5116 | 1.0578 | 1.2121 | 1.6849 | 0.9709 | 1.0443 | 1.1211 | 1.0739 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
16 | 174 | 1593 | 6 | 1726 | 77 | 1911 | 1512 | 1742 | 251 | 3504 | 1518 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1006.14 | 943.80 | 959.12 | 319.25 | 947.39 | 1166.30 | 1161.51 | 1055.83 | 947.93 | 1012.06 | 1069.50 | 1052.92 |
Standard Deviation | 197.21 | 166.76 | 106.11 | 2.12 | 119.31 | 277.98 | 186.80 | 188.43 | 120.39 | 231.32 | 185.21 | 193.65 |
Intensity Change Log 2 | — | -0.092278 | -0.069048 | -1.656073 | — | 0.299909 | 0.293971 | 0.156347 | — | 0.111228 | 0.118410 | -0.506568 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.116197* | 0.580311* | 0.401750* | 0.000106 | 0.478338* | 0.569226* | 0.663867* | 0.448246* | 0.475012* | 0.576910* | 0.544703* | 0.446475* |
Bud Neck | 0.012183 | 0.009320 | 0.052113 | 0.000008 | 0.033331 | 0.004353 | 0.024392 | 0.022433 | 0.033137 | 0.007796 | 0.036995 | 0.022344 |
Bud Site | 0.103982* | 0.072093 | 0.307207* | 0.000037 | 0.133427* | 0.037643 | 0.161973* | 0.120515* | 0.133157* | 0.061525 | 0.228000* | 0.120039* |
Cell Periphery | 0.011090 | 0.013977 | 0.022214 | 0.000477 | 0.035233 | 0.000648 | 0.021104 | 0.003063 | 0.035011 | 0.009888 | 0.021609 | 0.003052 |
Cytoplasm | 0.084305 | 0.021691 | 0.096455 | 0.000084 | 0.127524* | 0.001937 | 0.021060 | 0.009017 | 0.127127* | 0.015631 | 0.055336 | 0.008982 |
Cytoplasmic Foci | 0.047751 | 0.106408 | 0.027932 | 0.000014 | 0.022548 | 0.075831 | 0.016227 | 0.127777 | 0.022779 | 0.097028 | 0.021548 | 0.127272 |
Eisosomes | 0.001007 | 0.018018 | 0.014662 | 0.001630 | 0.063168 | 0.019924 | 0.011968 | 0.002221 | 0.062597 | 0.018603 | 0.013193 | 0.002219 |
Endoplasmic Reticulum | 0.008534 | 0.002935 | 0.008559 | 0.000001 | 0.005766 | 0.001109 | 0.017972 | 0.001316 | 0.005792 | 0.002375 | 0.013693 | 0.001310 |
Endosome | 0.096546 | 0.017799 | 0.006421 | 0.000000 | 0.004157 | 0.014030 | 0.008024 | 0.013751 | 0.005006 | 0.016642 | 0.007295 | 0.013697 |
Golgi | 0.024281 | 0.027057 | 0.004798 | 0.000004 | 0.013555 | 0.022309 | 0.005214 | 0.010261 | 0.013654 | 0.025600 | 0.005025 | 0.010221 |
Lipid Particles | 0.002829 | 0.028243 | 0.005846 | 0.000025 | 0.008413 | 0.050061 | 0.017479 | 0.029232 | 0.008361 | 0.034936 | 0.012191 | 0.029117 |
Mitochondria | 0.025681 | 0.004174 | 0.001951 | 0.000018 | 0.009287 | 0.004562 | 0.001450 | 0.003199 | 0.009438 | 0.004293 | 0.001678 | 0.003186 |
Mitotic Spindle | 0.007239 | 0.001278 | 0.002208 | 0.000181 | 0.000166 | 0.018871 | 0.002079 | 0.011781 | 0.000231 | 0.006675 | 0.002137 | 0.011735 |
None | 0.000706 | 0.000128 | 0.000147 | 0.997044* | 0.000259 | 0.000199 | 0.000237 | 0.000100 | 0.000263 | 0.000150 | 0.000196 | 0.004041 |
Nuclear Periphery | 0.001759 | 0.000013 | 0.000019 | 0.000000 | 0.000011 | 0.000056 | 0.000027 | 0.000067 | 0.000027 | 0.000026 | 0.000023 | 0.000067 |
Nuclear Periphery Foci | 0.005740 | 0.000213 | 0.000416 | 0.000001 | 0.000130 | 0.000242 | 0.000496 | 0.000593 | 0.000181 | 0.000222 | 0.000460 | 0.000591 |
Nucleolus | 0.070521 | 0.000045 | 0.000040 | 0.000001 | 0.000029 | 0.000021 | 0.000012 | 0.000070 | 0.000677 | 0.000038 | 0.000025 | 0.000069 |
Nucleus | 0.078798 | 0.000852 | 0.005807 | 0.000007 | 0.004325 | 0.000045 | 0.000765 | 0.000149 | 0.005009 | 0.000605 | 0.003057 | 0.000148 |
Peroxisomes | 0.028127 | 0.092041 | 0.023292 | 0.000362 | 0.056379 | 0.177550* | 0.018052 | 0.194440* | 0.056120 | 0.118273* | 0.020434 | 0.193673* |
Vacuole | 0.233982* | 0.003225 | 0.017990 | 0.000000 | 0.003637 | 0.001148 | 0.007461 | 0.001619 | 0.005753 | 0.002588 | 0.012248 | 0.001613 |
Vacuole Periphery | 0.038745 | 0.000181 | 0.000172 | 0.000000 | 0.000316 | 0.000236 | 0.000139 | 0.000149 | 0.000669 | 0.000198 | 0.000154 | 0.000149 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | NaN | NaN | NaN | NaN | NaN | -2.02 | -16.28 | 2.54 | 2.72 | 16.66* | -3.98 | -6.57 | 2.42 | 5.05 | 8.17 |
Bud Neck | NaN | NaN | NaN | NaN | NaN | 13.33 | 3.88 | 4.49 | -9.89 | 0.89 | 11.11 | -1.50 | 4.48 | -7.41 | 6.79 |
Bud Site | NaN | NaN | NaN | NaN | NaN | 5.99 | -3.95 | 1.73 | -5.19 | 5.44 | 6.75 | -14.13 | 1.76 | -5.48 | 15.31 |
Cell Periphery | NaN | NaN | NaN | NaN | NaN | 16.29 | 5.49 | 14.86 | -4.14 | 11.09 | 6.82 | 5.71 | 14.87 | 2.21 | 14.72 |
Cytoplasm | NaN | NaN | NaN | NaN | NaN | 28.33 | 21.62 | 26.53 | -8.93 | 6.89 | 20.41 | 14.16 | 26.62 | 1.96 | 20.37 |
Cytoplasmic Foci | NaN | NaN | NaN | NaN | NaN | -2.61 | 2.60 | -18.44 | -2.51 | -19.39 | -5.74 | 0.21 | -18.37 | -2.22 | -18.62 |
Eisosomes | NaN | NaN | NaN | NaN | NaN | 5.99 | 18.27 | 22.99 | 2.64 | 9.66 | 9.75 | 18.23 | 22.95 | 4.44 | 14.16 |
Endoplasmic Reticulum | NaN | NaN | NaN | NaN | NaN | 5.70 | -6.61 | 6.87 | -0.38 | 9.55 | 4.27 | -6.55 | 6.97 | 2.06 | 11.81 |
Endosome | NaN | NaN | NaN | NaN | NaN | -1.48 | -4.48 | -6.67 | 0.04 | -3.74 | -3.53 | -2.88 | -5.88 | 0.84 | -4.43 |
Golgi | NaN | NaN | NaN | NaN | NaN | -1.44 | 6.62 | 2.61 | 2.02 | -5.56 | -2.64 | 7.47 | 2.73 | 3.48 | -6.83 |
Lipid Particles | NaN | NaN | NaN | NaN | NaN | -2.57 | -5.54 | -8.13 | 1.27 | -3.93 | -3.71 | -3.48 | -8.14 | 0.77 | -6.35 |
Mitochondria | NaN | NaN | NaN | NaN | NaN | 2.65 | 9.43 | 7.08 | 0.81 | -2.94 | 3.49 | 9.95 | 7.25 | 0.82 | -2.94 |
Mitotic Spindle | NaN | NaN | NaN | NaN | NaN | -1.63 | -3.48 | -7.28 | 0.54 | -5.81 | -1.81 | -4.10 | -7.24 | -1.46 | -5.84 |
None | NaN | NaN | NaN | NaN | NaN | 1.04 | 0.04 | 6.18 | 1.74 | 1.23 | 2.84 | 0.30 | -2.35 | -2.42 | -2.37 |
Nuclear Periphery | NaN | NaN | NaN | NaN | NaN | -2.21 | -6.26 | -4.50 | -0.52 | -2.97 | 0.05 | 0.12 | -2.23 | -2.91 | -3.32 |
Nuclear Periphery Foci | NaN | NaN | NaN | NaN | NaN | -0.82 | -3.78 | -3.50 | -1.91 | -0.32 | -0.51 | -3.74 | -2.97 | -2.59 | -0.67 |
Nucleolus | NaN | NaN | NaN | NaN | NaN | 1.16 | 3.87 | -2.27 | -2.56 | -3.09 | 1.28 | 1.28 | 1.22 | -1.29 | -1.13 |
Nucleus | NaN | NaN | NaN | NaN | NaN | 11.49 | 8.84 | 11.10 | -3.22 | 6.87 | 5.96 | 2.37 | 7.54 | 1.20 | 9.84 |
Peroxisomes | NaN | NaN | NaN | NaN | NaN | -3.98 | 13.21 | -19.02 | -0.51 | -25.14* | -4.70 | 13.38 | -19.01 | -5.04 | -25.07* |
Vacuole | NaN | NaN | NaN | NaN | NaN | 3.01 | -4.44 | 3.88 | -0.66 | 6.25 | 2.55 | -6.13 | 4.29 | 1.06 | 11.79 |
Vacuole Periphery | NaN | NaN | NaN | NaN | NaN | 0.39 | 2.76 | 2.43 | 0.43 | -0.28 | 2.39 | 2.78 | 2.77 | 0.61 | 0.10 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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