Standard name
Human Ortholog
Description Twin Cx(9)C protein involved in cytochrome c oxidase organization; organization includes assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.99 0.9 0.86 – 0.72 0.66 0.64 0.68 0.66 0.98 0.98 0.98 0.94 0.91 0.95 0.96 0.94 0.95 0.91 0.93 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.13 0.06 0.17 – 0.18 0.36 0.32 0.36 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 – 0.17 0.12 0.16 0.09 0.1 0 0 0 0.1 0.12 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 1 0 0 2 1 0 – 1 2 3 1 7 0 0 1 0 0 0 1 0 0 0 0 4
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 2 0 0 2 0 – 2 2 9 0 8 1 1 1 3 3 1 0 0 0 0 0 0
Cytoplasm 370 144 109 154 207 203 – 174 156 237 154 232 208 326 423 165 119 166 372 137 104 98 189 203
Endoplasmic Reticulum 2 1 0 0 0 1 – 1 2 3 0 2 0 0 1 5 4 2 0 1 0 0 0 2
Endosome 1 0 0 0 3 0 – 0 0 0 0 0 0 0 0 1 2 1 2 0 0 1 0 4
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 1 1
Mitochondria 2 0 7 21 13 40 – 43 84 119 81 136 0 2 3 0 2 4 0 2 1 0 1 2
Nucleus 2 0 1 0 1 2 – 2 9 13 6 10 6 2 6 2 0 1 1 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 – 2 2 1 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 – 0 0 1 1 1 0 0 0 1 2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1
Vac/Vac Membrane 1 2 0 4 14 9 – 42 29 61 21 34 4 1 5 18 16 14 3 0 1 3 5 3
Unique Cell Count 377 148 112 156 229 235 240 235 371 225 353 213 332 433 176 131 175 387 146 110 108 204 231
Labelled Cell Count 382 149 117 181 242 255 267 286 447 265 433 219 334 441 195 148 189 387 146 110 108 204 231


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 8.1 4.1 4.9 6.9 6.3 – 6.9 5.9 6.3 6.1 6.0 7.1 7.3 7.6 15.8 15.0 15.1 5.8 7.0 7.1
Std Deviation (1e-4) 0.8 1.3 1.1 1.2 1.5 1.2 – 1.8 1.4 1.2 1.6 1.3 5.7 1.2 1.4 3.6 3.2 3.2 1.2 1.4 1.4
Intensity Change (Log2) – – – 0.26 0.75 0.6 – 0.74 0.52 0.61 0.57 0.53 0.78 0.82 0.88 1.94 1.86 1.87 0.5 0.76 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 -2.3 -3.2 – -5.5 -6.3 -6.9 -6.0 -6.6 0.2 0.6 0.2 -1.4 -2.1 -1.0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.9 -0.2 2.7 – 2.9 5.8 5.5 5.9 6.4 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 4.7 3.9 4.6 3.3 3.4 0 0 0 3.5 3.8 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5173 3.4647 3.0159 2.7576 2.6148 2.862 1.6179 2.4462 2.1616 1.6162 1.5188 1.7998 3.5844 4.7684 4.1447 3.9528 4.1027 3.8746
Actin 0.0027 0.0003 0.0212 0.0006 0.0002 0.0078 0.0226 0.0012 0.0056 0.0089 0.0001 0.0006 0.0232 0.0003 0.0127 0.0151 0.0918 0.0021
Bud 0.0002 0.0001 0.0004 0.0002 0.0002 0.0007 0.0006 0.0007 0.0071 0.0003 0.0002 0.0003 0.0003 0.0001 0.0002 0.0005 0.0002 0.0002
Bud Neck 0.0003 0.0001 0.0011 0.0002 0.0003 0.0019 0.001 0.0002 0.0005 0.0005 0.0002 0.001 0.0018 0.0001 0.0007 0.0005 0.0005 0.0008
Bud Periphery 0.0001 0 0.0002 0.0001 0 0.0017 0.0005 0.0002 0.0029 0.0002 0.0001 0.0001 0.0003 0 0.0002 0.0003 0.0002 0.0004
Bud Site 0.0011 0.0003 0.0029 0.0001 0 0.0008 0.0037 0.0053 0.0024 0.0006 0 0.0002 0.0016 0.0002 0.003 0.0005 0.0004 0.0003
Cell Periphery 0.0001 0.0001 0.0001 0 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0 0.0001 0 0 0
Cytoplasm 0.787 0.9228 0.7938 0.8829 0.9012 0.8742 0.7314 0.8718 0.8096 0.8762 0.9562 0.9188 0.7722 0.9404 0.8335 0.8549 0.8177 0.9206
Cytoplasmic Foci 0.0163 0.0035 0.0252 0.0041 0.0025 0.0119 0.0448 0.0037 0.0159 0.0107 0.0052 0.0124 0.0314 0.0016 0.0077 0.0071 0.0115 0.008
Eisosomes 0.0001 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0 0.0001 0 0.0002 0.0001 0.0002 0
Endoplasmic Reticulum 0.006 0.0031 0.0049 0.0032 0.0017 0.002 0.0071 0.0017 0.004 0.0032 0.001 0.0027 0.0041 0.0013 0.002 0.003 0.0005 0.0015
Endosome 0.0197 0.0017 0.0166 0.0018 0.0011 0.0189 0.0282 0.0012 0.0074 0.0101 0.0032 0.0043 0.022 0.0004 0.0023 0.0029 0.0025 0.0086
Golgi 0.008 0.0002 0.0073 0.0001 0 0.0123 0.0119 0.0002 0.0044 0.0158 0.0001 0.0001 0.0033 0 0.0013 0.002 0.007 0.0021
Lipid Particles 0.0047 0.0002 0.0065 0.0001 0 0.0071 0.019 0.0001 0.0016 0.0076 0 0.0001 0.0111 0 0.0031 0.0049 0.0146 0.0047
Mitochondria 0.0082 0.0001 0.0016 0.0003 0.0001 0.0138 0.009 0.0001 0.0047 0.0357 0.0001 0.0002 0.0013 0.0001 0.0007 0.0011 0.0008 0.0015
None 0.1168 0.0625 0.0919 0.0947 0.0856 0.025 0.0923 0.1093 0.115 0.0136 0.0285 0.0502 0.0857 0.0521 0.1183 0.0869 0.0063 0.0255
Nuclear Periphery 0.0055 0.0008 0.0032 0.0025 0.0013 0.0025 0.0038 0.0007 0.0024 0.0013 0.0005 0.0011 0.0032 0.0005 0.0011 0.0019 0.0006 0.0021
Nucleolus 0.0004 0 0.0001 0 0 0.0005 0.0005 0 0.0004 0.0001 0 0 0.0012 0 0.0002 0.0001 0 0.0003
Nucleus 0.0102 0.0034 0.0032 0.007 0.0045 0.0022 0.0068 0.0022 0.0062 0.0032 0.0021 0.0053 0.0078 0.0023 0.0033 0.0061 0.0042 0.0089
Peroxisomes 0.0048 0 0.0168 0.0001 0 0.0035 0.01 0.0001 0.0014 0.0098 0 0.0001 0.0127 0 0.0064 0.0092 0.0383 0.0013
Punctate Nuclear 0.0058 0.0003 0.0016 0.0012 0.0003 0.0084 0.0037 0.0008 0.007 0.0006 0.0003 0.0014 0.0129 0.0002 0.0026 0.0016 0.0023 0.0101
Vacuole 0.0016 0.0005 0.0008 0.0006 0.0007 0.0027 0.0024 0.0004 0.001 0.001 0.0017 0.0008 0.0028 0.0002 0.0004 0.0009 0.0001 0.0007
Vacuole Periphery 0.0005 0.0001 0.0003 0.0001 0.0002 0.0016 0.0005 0.0001 0.0002 0.0004 0.0004 0.0002 0.0008 0 0.0001 0.0002 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.4358 6.5323 12.926 10.504 4.7371 14.6065 9.3568 10.4015 25.2084 10.3598
Translational Efficiency 6.433 1.6522 0.7134 1.9607 3.3308 1.6593 1.6707 2.7792 1.0618 2.1004

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1758 1185 339 1391 1427 1665 1218 1422 3185 2850 1557 2813

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 770.07 833.43 1298.86 1153.19 730.16 837.11 1140.60 1178.58 752.19 835.58 1175.06 1166.02
Standard Deviation 92.10 112.04 181.00 180.29 105.85 108.87 146.71 198.23 100.48 110.22 168.04 190.00
Intensity Change Log 2 — 0.114071 0.754184 0.582569 — 0.197205 0.643508 0.690765 — 0.155131 0.701378 0.636242

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000107 0.000285 0.000937 0.004505 0.000112 0.000459 0.000873 0.004829 0.000109 0.000386 0.000887 0.004668
Bud Neck 0.008590 0.014373 0.006271 0.006667 0.004540 0.015208 0.005393 0.004732 0.006775 0.014861 0.005584 0.005689
Bud Site 0.003656 0.010558 0.008863 0.040767 0.003680 0.011529 0.018807 0.025817 0.003667 0.011125 0.016642 0.033210
Cell Periphery 0.000061 0.000094 0.000218 0.000177 0.000091 0.000100 0.000102 0.000139 0.000075 0.000097 0.000128 0.000158
Cytoplasm 0.657181* 0.577518* 0.341400* 0.561116* 0.618186* 0.599372* 0.520420* 0.668437* 0.639710* 0.590286* 0.481443* 0.615368*
Cytoplasmic Foci 0.094066 0.140567 0.006228 0.021097 0.118585 0.155390 0.005091 0.015820 0.105052 0.149227 0.005339 0.018429
Eisosomes 0.000050 0.000049 0.000022 0.000036 0.000051 0.000083 0.000020 0.000031 0.000050 0.000069 0.000021 0.000033
Endoplasmic Reticulum 0.000401 0.001102 0.003736 0.002060 0.000881 0.000521 0.002005 0.001026 0.000616 0.000762 0.002382 0.001537
Endosome 0.003575 0.010825 0.001280 0.011501 0.004826 0.011051 0.001191 0.010838 0.004135 0.010957 0.001210 0.011166
Golgi 0.000859 0.002554 0.000191 0.009970 0.001425 0.001938 0.000134 0.011527 0.001112 0.002195 0.000147 0.010757
Lipid Particles 0.001651 0.002531 0.001136 0.001654 0.002493 0.002149 0.000918 0.001997 0.002028 0.002308 0.000966 0.001827
Mitochondria 0.001370 0.001397 0.000661 0.008746 0.004511 0.004557 0.000897 0.006323 0.002778 0.003243 0.000846 0.007521
Mitotic Spindle 0.000426 0.006232 0.002412 0.060218 0.002752 0.005029 0.031394 0.028175 0.001468 0.005530 0.025084 0.044020
None 0.002971 0.001965 0.006586 0.005682 0.003202 0.002173 0.008799 0.009396 0.003074 0.002086 0.008318 0.007559
Nuclear Periphery 0.000201 0.000483 0.001111 0.001323 0.000350 0.000222 0.001321 0.000450 0.000268 0.000330 0.001275 0.000882
Nuclear Periphery Foci 0.000247 0.000746 0.002065 0.003028 0.000660 0.000534 0.001254 0.002269 0.000432 0.000622 0.001430 0.002644
Nucleolus 0.000443 0.000708 0.000205 0.000273 0.000861 0.000584 0.000192 0.000153 0.000630 0.000635 0.000195 0.000212
Nucleus 0.177379* 0.153776* 0.564275* 0.136697* 0.168184* 0.106346 0.326720* 0.125844 0.173259* 0.126067 0.378442* 0.131211*
Peroxisomes 0.001597 0.003291 0.000157 0.003521 0.001472 0.005245 0.000601 0.001067 0.001541 0.004433 0.000504 0.002280
Vacuole 0.044904 0.069933 0.052098 0.114156 0.062589 0.076475 0.073732 0.077554 0.052827 0.073755 0.069021 0.095653
Vacuole Periphery 0.000266 0.001013 0.000148 0.006807 0.000549 0.001036 0.000134 0.003578 0.000393 0.001026 0.000137 0.005175

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.50 -9.78 -3.42 -2.93 -0.06 -2.29 -4.35 -2.06 -1.43 -1.31 -2.95 -7.30 -3.55 -2.68 -1.75
Bud Neck -2.99 1.23 5.45 5.93 2.59 -7.23 -1.20 2.70 8.52 3.94 -6.81 1.66 6.19 10.26 4.32
Bud Site -5.73 -2.17 -5.79 -1.63 -2.02 -6.12 -3.38 -4.39 0.96 -0.49 -8.20 -3.95 -7.24 -0.45 -1.83
Cell Periphery -5.83 -10.94 -10.25 -4.47 7.75 -0.99 -2.78 -0.00 0.81 2.28 -4.83 -11.29 -6.01 -2.20 5.56
Cytoplasm 8.85 28.38 20.19 9.00 -14.44 1.77 22.73 6.76 5.12 -16.54 7.65 35.77 18.95 10.36 -19.10
Cytoplasmic Foci -7.66 27.28 25.39 25.87 -5.78 -6.35 30.35 28.09 33.22 -11.59 -10.64 41.10 37.67 42.04 -13.26
Eisosomes 0.01 4.80 6.19 6.47 0.40 -5.24 9.55 10.68 10.43 2.91 -4.88 10.75 11.60 11.77 1.55
Endoplasmic Reticulum -7.92 -9.22 -8.65 -2.06 7.04 3.17 -10.63 1.75 -2.15 13.05 -2.02 -15.68 -5.83 -3.47 12.66
Endosome -7.72 4.23 1.39 7.87 -1.27 -7.33 10.85 3.89 9.08 -1.96 -10.79 10.93 3.56 12.01 -2.78
Golgi -2.18 4.66 -1.22 0.76 -2.13 -0.98 2.82 -0.67 -0.04 -2.70 -2.58 4.43 -1.37 0.53 -3.51
Lipid Particles -2.22 -0.73 -0.45 2.46 0.50 0.96 3.60 3.20 2.88 -0.08 -1.03 2.56 1.99 3.72 -0.76
Mitochondria 0.05 1.07 -0.41 -0.50 -1.15 -0.05 2.81 2.41 3.12 -0.81 -0.55 2.96 1.69 2.44 -1.36
Mitotic Spindle -3.81 -3.92 -8.45 -5.59 -6.80 -1.58 -7.68 -6.04 -4.77 2.66 -4.03 -8.46 -10.48 -7.43 0.46
None 2.30 -3.67 -4.47 -6.98 -1.08 1.47 -11.20 -10.19 -8.36 1.22 2.22 -11.82 -9.74 -10.18 2.24
Nuclear Periphery -7.87 -15.78 -8.13 -5.99 2.36 5.44 -28.22 -14.88 -19.83 19.66 -3.50 -33.10 -11.74 -10.67 12.25
Nuclear Periphery Foci -6.54 -8.55 -13.65 -9.54 1.03 1.40 -6.04 -8.68 -9.22 -2.91 -3.12 -11.06 -16.03 -13.40 -2.96
Nucleolus -3.00 3.65 2.64 4.71 -0.79 1.29 2.99 3.49 6.68 4.87 -0.43 3.89 4.30 7.63 1.83
Nucleus 3.50 -27.54 -0.33 -3.74 27.34 10.17 -26.08 -0.54 -12.31 27.18 10.75 -35.57 -0.52 -11.75 35.76
Peroxisomes -3.95 4.65 2.11 6.19 -1.81 -5.73 5.25 4.51 7.89 -0.55 -6.73 6.45 4.15 9.55 -1.69
Vacuole -8.30 -6.80 -30.48 -24.11 -15.25 -3.03 -12.57 -21.24 -19.45 -8.18 -8.57 -16.25 -36.84 -30.83 -15.39
Vacuole Periphery -3.44 3.07 -1.64 -0.32 -1.79 -3.17 4.95 -0.23 1.06 -1.45 -5.14 6.38 -1.54 0.32 -2.36
Standard name
Human Ortholog
Description Twin Cx(9)C protein involved in cytochrome c oxidase organization; organization includes assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors
Localization
Cell Percentages cytoplasm (83%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Coa4

Coa4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Coa4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available