Standard name
Human Ortholog
Description Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.92 0.85 0.88 0.73 0.71 0.42 0.25 0.29 0.15 0.15 0.24 0.9 0.9 0.79 0.88 0.78 0.81 0.68 0.58 0.7 0.76 0.73 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.12 0.09 0.16 0.36 0.3 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.06 0.11 0.18 0.11 0.14 0.13 0.14 0 0 0 0 0 0 0.21 0.28 0.08 0.12 0.12 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.1 0.2 0.13 0.28 0.26 0.44 0.55 0.5 0.49 0.52 0.46 0.14 0.18 0.33 0.25 0.38 0.32 0.05 0.09 0.08 0.09 0.07 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 4 0 0 3 4 5 0 0 0 0 1 0 1 1 1 1 5 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 1 2 1 2 0 3 1 1 0 1 1 0 1 0 0 0 0 0 0 0
Cytoplasm 126 84 204 175 151 200 156 102 62 45 30 56 239 414 356 21 56 30 97 63 174 155 367 293
Endoplasmic Reticulum 1 0 3 3 2 4 2 2 0 1 2 2 3 1 2 1 5 2 2 0 7 0 5 2
Endosome 0 0 2 0 2 2 3 12 5 4 2 0 0 0 0 0 1 1 0 0 6 1 7 7
Golgi 1 1 7 15 15 4 3 7 1 2 0 0 6 11 25 1 3 2 0 0 0 0 0 0
Mitochondria 1 0 1 0 3 14 46 37 35 109 60 71 0 0 0 0 0 0 0 0 1 0 0 0
Nucleus 0 0 4 0 3 16 43 74 23 41 26 33 2 1 3 0 0 0 30 31 19 25 58 46
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 6 0 9 9
Nucleolus 0 0 0 1 3 4 5 10 3 3 1 2 0 0 2 0 1 2 0 0 1 0 2 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 6 5
Vac/Vac Membrane 13 9 49 26 58 73 165 225 107 148 103 106 38 84 150 6 27 12 6 9 20 17 34 39
Unique Cell Count 136 91 240 200 207 282 374 410 216 303 199 232 267 461 448 24 72 37 144 109 250 206 501 416
Labelled Cell Count 142 94 270 221 240 319 429 475 236 360 230 276 288 512 539 29 95 49 144 109 250 206 501 416


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.0 32.5 22.8 22.7 18.2 14.5 12.5 12.3 10.8 9.7 9.2 9.3 28.9 28.0 25.9 30.8 31.8 34.2 28.7 30.0 31.0
Std Deviation (1e-4) 4.9 5.8 5.4 4.0 4.1 3.2 3.3 3.6 2.6 2.9 2.4 3.0 6.1 5.8 5.8 12.7 9.4 11.4 6.5 6.2 6.5
Intensity Change (Log2) – – – -0.01 -0.33 -0.65 -0.86 -0.89 -1.08 -1.23 -1.32 -1.29 0.34 0.3 0.19 0.43 0.48 0.59 0.34 0.4 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 -3.1 -3.8 -10.6 -14.8 -12.2 -16.3 -14.6 -13.3 1.5 1.9 -1.8 0.3 -1.4 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 1.8 0 0 0 0 0 0 0 0 0 0
Golgi 2.2 2.1 -1.2 0 -1.0 0 0 0 0 -0.5 -0.4 1.6 0 0 0
Mitochondria 0 0 3.1 5.4 4.5 6.2 10.2 9.0 9.1 0 0 0 0 0 0
Nucleus 0 0 2.4 4.5 6.2 4.1 5.0 4.7 5.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.1 1.9 1.5 6.0 8.6 6.5 6.8 6.9 5.8 -1.8 -0.7 3.6 0.5 3.0 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.9947 25.5074 23.3569 24.4432 21.3429 22.2772 21.6715 30.512 25.8155 23.9864 24.0419 27.201 21.7567 30.5445 25.7637 21.6925 19.7714 24.9523
Actin 0.0002 0.0001 0.0018 0.0002 0.0001 0.0007 0.0119 0.0008 0.0034 0.0066 0.0005 0.0035 0.0053 0.0001 0.0198 0.0146 0.0508 0.0061
Bud 0.0001 0.0001 0.0002 0 0.0001 0.0002 0.001 0.0001 0.0009 0.001 0.0007 0.0001 0.0012 0.0001 0.0007 0.0004 0.0009 0.0002
Bud Neck 0.0002 0.0003 0.0006 0.0002 0.0003 0.001 0.0014 0.0003 0.0015 0.0017 0.0006 0.0006 0.0007 0.0002 0.0005 0.0014 0.0008 0.0005
Bud Periphery 0 0 0.0001 0 0.0001 0.0001 0.0008 0.0001 0.0008 0.0011 0.0005 0.0001 0.0015 0 0.0004 0.0004 0.0009 0.0001
Bud Site 0.0001 0.0001 0.0004 0 0.0002 0.0001 0.0031 0.0002 0.0012 0.0024 0.0004 0.0001 0.003 0 0.0007 0.0028 0.0026 0.0001
Cell Periphery 0 0.0001 0.0001 0 0.0001 0.0001 0.0006 0 0.0002 0.0004 0.0001 0 0.0002 0 0.0001 0.0001 0.0003 0.0001
Cytoplasm 0.598 0.7106 0.6792 0.6344 0.6053 0.6677 0.623 0.8043 0.6981 0.7163 0.7282 0.76 0.6479 0.8766 0.7425 0.6829 0.6958 0.6565
Cytoplasmic Foci 0.0018 0.0015 0.0047 0.0002 0.0041 0.0022 0.027 0.0007 0.0107 0.0117 0.0122 0.0022 0.0149 0.0002 0.0112 0.0294 0.0161 0.0034
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0001 0.0004 0 0 0.0001 0 0.0002 0.0001 0.0003 0
Endoplasmic Reticulum 0.0037 0.0014 0.0025 0.0012 0.0036 0.0081 0.013 0.0008 0.0051 0.0043 0.0029 0.0023 0.0095 0.0003 0.0028 0.0004 0.0101 0.0035
Endosome 0.0035 0.0019 0.0057 0.0001 0.0154 0.0101 0.0225 0.0019 0.022 0.0259 0.0264 0.0068 0.0207 0.0002 0.0171 0.0145 0.0207 0.0104
Golgi 0.0001 0 0.0005 0 0.001 0.002 0.0085 0.0014 0.0034 0.0035 0.0031 0.0016 0.0041 0 0.0073 0.0151 0.0112 0.0031
Lipid Particles 0.0002 0.0003 0.0011 0 0.0031 0.0001 0.0041 0.0003 0.0101 0.0059 0.0024 0.0033 0.0114 0 0.0076 0.0184 0.0344 0.0016
Mitochondria 0.0003 0.0001 0.0003 0.0001 0.001 0.0011 0.0054 0.0012 0.0081 0.0163 0.0014 0.0024 0.0069 0.0001 0.0113 0.0672 0.0114 0.0015
None 0.0006 0.0001 0.0007 0.0001 0.001 0.0008 0.0032 0.0001 0.0011 0.0012 0.0007 0.0003 0.0023 0.0002 0.0018 0.0004 0.0045 0.0011
Nuclear Periphery 0.0751 0.0309 0.0454 0.0193 0.069 0.0551 0.048 0.0131 0.0272 0.0261 0.022 0.0298 0.0494 0.0082 0.0205 0.0091 0.0348 0.0447
Nucleolus 0.0003 0.0005 0.0012 0.0001 0.0002 0.0003 0.001 0.001 0.0016 0.0059 0.0003 0.0001 0.0011 0.0001 0.0005 0.0003 0.0005 0.0008
Nucleus 0.2986 0.2436 0.2381 0.3428 0.2832 0.2398 0.2011 0.1704 0.1828 0.1401 0.1672 0.1778 0.1994 0.1123 0.1335 0.0918 0.0735 0.2593
Peroxisomes 0 0 0.0014 0 0.0001 0 0.0035 0.0001 0.0016 0.0015 0.0008 0.0013 0.0021 0 0.0133 0.0445 0.0139 0.0002
Punctate Nuclear 0.0074 0.0006 0.0034 0.0005 0.0008 0.0018 0.0028 0.0004 0.0027 0.0044 0.0032 0.0012 0.003 0.0003 0.0029 0.0017 0.0034 0.0012
Vacuole 0.0075 0.0072 0.011 0.0005 0.009 0.0065 0.0141 0.0025 0.0126 0.0178 0.0183 0.0047 0.0108 0.0009 0.0036 0.0026 0.0098 0.0036
Vacuole Periphery 0.0022 0.0007 0.0017 0.0002 0.0024 0.0021 0.0038 0.0004 0.0048 0.0055 0.0081 0.0019 0.0044 0.0003 0.0019 0.0018 0.0035 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 166.3638 157.6168 120.7313 157.5538 146.8713 93.7943 146.2999 137.3432 111.2359 150.9904
Translational Efficiency 2.0193 2.2829 2.4011 1.5577 2.0651 3.2082 1.818 1.729 1.6798 1.7915

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1414 180 467 1384 2195 1229 290 1253 3609 1409 757 2637

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1815.10 2397.72 2862.68 2954.98 2090.46 2153.02 2897.07 2876.97 1982.57 2184.28 2875.85 2917.91
Standard Deviation 348.91 493.09 529.35 778.96 372.49 433.91 504.50 876.01 387.49 449.40 520.24 827.41
Intensity Change Log 2 — 0.401614 0.657317 0.703099 — 0.042541 0.470774 0.460730 — 0.220572 0.560477 0.578449

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000313 0.000631 0.000285 0.000131 0.000144 0.000068 0.000223 0.000236 0.000210 0.000140 0.000261 0.000181
Bud Neck 0.003150 0.003514 0.006911 0.001335 0.002859 0.001366 0.005429 0.001512 0.002973 0.001640 0.006344 0.001419
Bud Site 0.001160 0.002972 0.001985 0.005211 0.000766 0.000627 0.001852 0.003486 0.000921 0.000926 0.001934 0.004391
Cell Periphery 0.000120 0.000243 0.000732 0.001899 0.000083 0.000039 0.000592 0.001345 0.000097 0.000065 0.000678 0.001636
Cytoplasm 0.491971* 0.490895* 0.497695* 0.850513* 0.535537* 0.610728* 0.563313* 0.817684* 0.518468* 0.595419* 0.522833* 0.834914*
Cytoplasmic Foci 0.003750 0.010316 0.013560 0.003371 0.002896 0.001868 0.009590 0.007436 0.003230 0.002947 0.012039 0.005303
Eisosomes 0.000002 0.000003 0.000003 0.000002 0.000001 0.000001 0.000002 0.000002 0.000001 0.000001 0.000003 0.000002
Endoplasmic Reticulum 0.000827 0.001273 0.000445 0.000090 0.000629 0.000177 0.000154 0.000101 0.000707 0.000317 0.000334 0.000095
Endosome 0.000365 0.000314 0.001380 0.000979 0.000318 0.000648 0.000804 0.000999 0.000337 0.000605 0.001159 0.000988
Golgi 0.000618 0.000894 0.002593 0.003894 0.000518 0.001247 0.001593 0.007599 0.000557 0.001202 0.002210 0.005655
Lipid Particles 0.000341 0.000841 0.002431 0.002386 0.000277 0.000130 0.002582 0.004299 0.000302 0.000221 0.002489 0.003295
Mitochondria 0.000729 0.000606 0.000775 0.000300 0.000696 0.000548 0.000396 0.000903 0.000709 0.000555 0.000630 0.000586
Mitotic Spindle 0.003793 0.014298 0.007285 0.003488 0.003298 0.004959 0.007790 0.008348 0.003492 0.006152 0.007478 0.005797
None 0.008086 0.009106 0.006626 0.002940 0.006368 0.010654 0.007884 0.004726 0.007041 0.010456 0.007108 0.003789
Nuclear Periphery 0.000707 0.000523 0.001869 0.000137 0.000939 0.000586 0.001379 0.000238 0.000848 0.000578 0.001681 0.000185
Nuclear Periphery Foci 0.002919 0.003480 0.006211 0.002445 0.003341 0.001654 0.007105 0.003620 0.003176 0.001887 0.006553 0.003003
Nucleolus 0.000826 0.000397 0.000540 0.000168 0.000616 0.000508 0.000343 0.000307 0.000698 0.000494 0.000465 0.000234
Nucleus 0.474517* 0.451281* 0.408773* 0.069647 0.436319* 0.359348* 0.361379* 0.096387 0.451285* 0.371093* 0.390617* 0.082353
Peroxisomes 0.000035 0.000060 0.000047 0.000043 0.000018 0.000022 0.000029 0.000034 0.000025 0.000027 0.000040 0.000039
Vacuole 0.004912 0.007679 0.038698 0.047525 0.003680 0.003831 0.027187 0.038778 0.004163 0.004323 0.034288 0.043369
Vacuole Periphery 0.000858 0.000673 0.001156 0.003496 0.000697 0.000992 0.000373 0.001958 0.000760 0.000952 0.000856 0.002765

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.86 -2.53 3.19 1.69 7.69 4.13 -5.72 -2.20 -3.78 2.27 2.19 -6.18 0.88 -1.53 7.08
Bud Neck -1.20 -7.24 5.17 2.96 8.46 3.15 -6.32 3.20 -0.58 7.49 2.80 -9.27 5.65 1.32 10.91
Bud Site -2.21 -7.21 -4.31 -0.57 -0.25 2.23 -6.80 -7.25 -8.02 -0.35 0.21 -10.05 -7.23 -6.95 -0.32
Cell Periphery -3.64 -11.26 -6.55 -5.53 -1.29 5.56 -8.66 -5.24 -5.55 0.14 3.32 -14.36 -8.43 -8.68 -0.81
Cytoplasm 1.08 5.26 -34.72 -18.03 -31.61 -5.61 4.37 -27.05 -17.70 -19.13 -6.58 8.04 -44.39 -26.49 -36.04
Cytoplasmic Foci -3.39 -8.40 -1.20 3.13 8.11 3.93 -7.49 -7.19 -10.57 3.48 1.70 -10.95 -6.50 -7.74 8.21
Eisosomes -1.74 -7.93 2.33 2.29 8.92 2.63 -7.69 -3.81 -4.78 4.16 2.02 -11.27 -2.52 -3.69 9.37
Endoplasmic Reticulum -0.15 1.90 10.24 1.31 4.66 5.24 4.38 6.83 4.17 5.41 3.95 2.74 11.26 2.51 5.55
Endosome 0.32 -6.36 -1.84 -1.89 4.26 -0.97 -6.64 -2.36 -0.67 3.92 -0.91 -8.67 -2.94 -0.91 5.65
Golgi -0.99 -6.72 -3.38 -2.86 -0.20 -0.99 -6.63 -3.28 -2.65 -1.91 -1.03 -9.11 -4.61 -3.34 -1.58
Lipid Particles -2.43 -9.41 -8.53 -4.49 2.76 3.65 -7.76 -8.62 -8.99 1.20 1.28 -12.21 -11.99 -12.16 2.46
Mitochondria 1.22 -3.05 6.18 2.98 8.84 1.04 2.58 0.65 0.17 0.08 1.25 -1.64 2.36 1.09 2.85
Mitotic Spindle -1.44 -3.13 0.08 1.45 3.08 -1.95 -2.75 -3.23 -1.99 0.78 -2.38 -4.26 -2.54 -0.15 2.24
None -0.52 3.34 7.62 5.45 6.77 -6.79 0.25 5.23 9.90 2.88 -5.63 2.73 9.92 12.84 6.26
Nuclear Periphery 0.55 -9.64 9.27 3.46 12.01 3.00 -6.06 6.68 4.24 8.24 2.70 -10.97 10.47 6.89 14.45
Nuclear Periphery Foci -0.64 -4.34 0.63 1.03 5.35 2.50 -5.11 -0.45 -3.25 5.08 2.15 -6.43 0.38 -2.15 7.14
Nucleolus 3.01 1.13 5.19 3.73 8.47 -0.85 2.46 4.92 2.72 3.25 -0.68 2.34 7.24 2.95 8.06
Nucleus 0.62 4.81 46.31* 18.72 26.79 5.75 5.40 46.41* 30.91 16.16 7.14 6.22 66.79* 38.35 30.63
Peroxisomes -1.12 -4.90 0.58 1.38 4.97 0.45 -4.56 -2.54 -2.49 2.95 0.73 -6.89 -1.13 -1.55 5.82
Vacuole -3.16 -11.49 -17.81 -10.40 2.48 -2.26 -9.67 -18.31 -17.24 2.00 -2.96 -15.11 -25.69 -23.17 3.12
Vacuole Periphery 0.62 -1.06 -2.53 -2.84 -2.06 -0.39 0.53 -1.84 -0.78 -2.43 -0.20 -1.10 -3.15 -2.23 -2.58
Standard name
Human Ortholog
Description Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p
Localization
Cell Percentages cytoplasm (75%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Map1

Map1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Map1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available