Standard name
Human Ortholog
Description Minor sphingoid long-chain base kinase; possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules; LCB5 has a paralog, LCB4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.93 0.95 0.95 0.89 0.91 – 0.78 0.79 0.78 0.7 0.97 0.96 0.97 0.82 0.87 0.92 0.67 0.76 0.49 0.71 0.67 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.14 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.11 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.05 0 0.08 0.12 – 0.27 0.22 0.34 0.39 0 0 0 0.07 0 0.05 0.15 0.07 0.18 0 0 0.05
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0.06 0.05 0 0.05 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 4 0 3 1
Bud 0 0 0 0 0 1 1 – 2 0 1 2 0 0 0 0 0 0 0 0 3 0 5 2
Bud Neck 0 0 3 1 3 3 3 – 2 3 2 2 0 0 0 0 0 0 0 0 1 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 1 0 1 0 – 1 1 0 0 0 0 0 0 1 1 1 0 0 0 0 0
Cytoplasm 89 88 89 59 123 143 156 – 83 64 50 47 69 145 189 23 52 76 75 89 55 29 79 101
Endoplasmic Reticulum 0 1 1 1 1 3 1 – 1 0 0 1 2 0 1 4 4 3 2 0 1 1 4 2
Endosome 0 0 1 0 0 2 3 – 0 0 0 0 0 2 0 3 3 1 1 3 4 1 3 5
Golgi 0 1 0 1 0 0 0 – 0 1 0 0 0 2 2 0 2 2 1 3 4 0 3 2
Mitochondria 5 1 0 3 0 13 20 – 29 18 22 26 0 0 0 2 1 4 17 7 20 1 3 6
Nucleus 0 0 3 0 1 2 2 – 3 1 0 0 0 3 1 0 1 1 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 1
Nucleolus 1 0 0 0 0 0 0 – 0 1 2 0 0 0 0 1 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 3 5 – 1 1 0 1 0 0 2 0 1 0 3 1 3 3 1 4
Vac/Vac Membrane 0 1 3 0 4 1 3 – 3 1 0 0 0 1 0 2 2 1 6 5 4 2 4 8
Unique Cell Count 93 92 96 62 129 160 172 107 81 64 67 71 151 194 28 60 83 114 118 113 41 119 144
Labelled Cell Count 95 93 103 66 134 173 194 125 91 78 79 71 153 195 35 69 91 114 118 113 41 119 144


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.2 5.2 5.4 6.3 5.0 5.2 – 3.6 4.0 3.2 3.7 5.1 5.9 5.2 5.8 7.8 7.1 – 5.8 5.5
Std Deviation (1e-4) 0.5 1.3 2.2 3.5 3.9 3.7 4.0 – 1.7 2.0 1.5 2.0 1.7 2.5 2.0 1.9 4.2 3.5 – 2.4 2.3
Intensity Change (Log2) – – – 0.04 0.26 -0.06 -0.0 – -0.53 -0.41 -0.72 -0.51 -0.03 0.18 -0.02 0.15 0.58 0.44 – 0.15 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.5373 -1.5088 -1.7593 -1.9525 -2.0626 -1.0905 -0.845 -0.5756 -0.6156 0.0178 -0.7346 -0.6158 -0.1235 -0.954 -1.1184 -0.2618 -0.5496 -0.886
Actin 0.0367 0.0047 0.0027 0.0512 0.0052 0.0285 0.0118 0.0068 0.0012 0.0023 0.0098 0.001 0.0722 0.0081 0.036 0.0418 0.0849 0.0223
Bud 0.0009 0.0001 0.0026 0.0004 0.0045 0.0003 0.0005 0.0005 0.0025 0.0049 0.0007 0.0001 0.0013 0.0042 0.0071 0.0009 0.0031 0.001
Bud Neck 0.007 0.0005 0.0006 0.0043 0.001 0.0007 0.009 0.0019 0.0045 0.026 0.0005 0.0016 0.0032 0.0009 0.005 0.001 0.0112 0.0011
Bud Periphery 0.0015 0.0001 0.0137 0.0008 0.0085 0.0003 0.0008 0.0016 0.0024 0.0069 0.0025 0.0001 0.004 0.0086 0.0074 0.0014 0.0101 0.0031
Bud Site 0.0107 0.0127 0.0003 0.0045 0.0016 0.0006 0.0212 0.0095 0.0142 0.0816 0.0019 0.0012 0.0028 0.023 0.0241 0.0031 0.0073 0.0006
Cell Periphery 0.0009 0.0002 0.0002 0.0006 0.0005 0.0002 0.0004 0.0006 0.0004 0.0019 0.0003 0.0001 0.0008 0.0006 0.0006 0.0003 0.0006 0.0001
Cytoplasm 0.0101 0.0145 0.0351 0.0094 0.0216 0.0732 0.0089 0.0145 0.0294 0.0111 0.0182 0.0177 0.0378 0.0374 0.0453 0.1167 0.0416 0.0698
Cytoplasmic Foci 0.02 0.0034 0.007 0.0097 0.024 0.0309 0.0344 0.002 0.0184 0.0174 0.0037 0.0141 0.0246 0.0135 0.0175 0.0127 0.0462 0.0068
Eisosomes 0.0007 0.0007 0.0005 0.0026 0.0003 0.0002 0.0005 0.0005 0.0003 0.0003 0.0003 0.0002 0.0009 0.0003 0.0004 0.0004 0.0006 0.0002
Endoplasmic Reticulum 0.0059 0.0009 0.0009 0.0008 0.0038 0.001 0.0009 0.0043 0.0041 0.0029 0.0004 0.0008 0.02 0.0012 0.0014 0.0059 0.0036 0.0015
Endosome 0.0333 0.0036 0.0058 0.0105 0.0692 0.0366 0.0237 0.0032 0.0022 0.0734 0.0045 0.005 0.037 0.0162 0.0103 0.0369 0.033 0.0049
Golgi 0.0113 0.0007 0.001 0.0097 0.0416 0.0369 0.0114 0.0009 0.0007 0.0187 0.0038 0.0012 0.0139 0.0022 0.006 0.0246 0.0215 0.0023
Lipid Particles 0.0127 0.0129 0.0125 0.0272 0.0063 0.0276 0.0202 0.0007 0.0016 0.0101 0.0227 0.0122 0.0165 0.011 0.0026 0.0183 0.0173 0.0013
Mitochondria 0.0058 0.001 0.0009 0.0378 0.1138 0.0481 0.0163 0.0007 0.0125 0.0068 0.0101 0.0105 0.0088 0.0025 0.0081 0.0145 0.0045 0.0019
None 0.7926 0.9413 0.907 0.8157 0.6659 0.6807 0.7985 0.9484 0.8893 0.6569 0.9099 0.9093 0.685 0.8531 0.8075 0.6987 0.6275 0.8669
Nuclear Periphery 0.0109 0.0002 0.0004 0.0005 0.0021 0.0043 0.0014 0.0007 0.0013 0.0045 0.0008 0.0061 0.0249 0.0014 0.0012 0.005 0.0212 0.0043
Nucleolus 0.0063 0.0001 0.0038 0.0005 0.0045 0.0005 0.0024 0.0004 0.0012 0.0095 0.0005 0.0015 0.001 0.0011 0.0005 0.0002 0.0012 0.0004
Nucleus 0.0109 0.0001 0.0006 0.0003 0.0027 0.0006 0.0022 0.0006 0.0026 0.0099 0.0004 0.0068 0.014 0.0013 0.0018 0.0014 0.0027 0.0013
Peroxisomes 0.0093 0.0014 0.0014 0.0095 0.0042 0.0163 0.0244 0.0001 0.0024 0.0025 0.0067 0.0014 0.0177 0.0035 0.0126 0.0089 0.0539 0.0008
Punctate Nuclear 0.0047 0.0002 0.0007 0.0022 0.0091 0.0103 0.0056 0.0007 0.0072 0.0183 0.0009 0.0078 0.0053 0.0074 0.0025 0.0042 0.0044 0.0085
Vacuole 0.0059 0.0003 0.0019 0.0012 0.0057 0.0015 0.0048 0.0011 0.001 0.0293 0.001 0.0005 0.0048 0.002 0.0015 0.0019 0.0021 0.0007
Vacuole Periphery 0.0018 0.0001 0.0003 0.0007 0.004 0.0009 0.0007 0.0002 0.0004 0.0048 0.0006 0.0008 0.0035 0.0005 0.0007 0.0012 0.0017 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5033 12.4253 15.6032 13.7842 13.1694 13.3575 19.2072 20.2199 19.8325 18.1899
Translational Efficiency 0.6341 0.9679 0.478 0.6669 0.7086 0.9035 0.727 0.7175 0.7101 0.8023

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1420 99 277 1412 212 1202 817 2154 1632 1301 1094 3566

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 589.87 793.02 751.74 773.22 655.30 670.61 782.26 764.26 598.37 679.92 774.53 767.81
Standard Deviation 73.05 1002.72 79.33 133.79 76.55 118.57 77.88 114.81 76.73 300.92 79.37 122.76
Intensity Change Log 2 — 0.426960 0.349837 0.390482 — 0.033318 0.255493 0.221908 — 0.233208 0.300956 0.304225

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000059 0.001799 0.000412 0.001488 0.000093 0.000740 0.000120 0.001018 0.000063 0.000821 0.000194 0.001204
Bud Neck 0.016266 0.034938 0.005646 0.023363 0.019008 0.036270 0.003658 0.017912 0.016622 0.036169 0.004162 0.020070
Bud Site 0.004499 0.032813 0.005839 0.065781 0.003354 0.036320 0.010519 0.065333 0.004350 0.036053 0.009334 0.065510
Cell Periphery 0.000212 0.000258 0.000163 0.000222 0.000175 0.000270 0.000109 0.000187 0.000207 0.000269 0.000122 0.000201
Cytoplasm 0.513144* 0.212012* 0.173285* 0.376925* 0.369445* 0.317768* 0.368989* 0.449955* 0.494477* 0.309720* 0.319437* 0.421038*
Cytoplasmic Foci 0.188501* 0.316865* 0.016792 0.017589 0.311998* 0.241739* 0.014333 0.014274 0.204544* 0.247456* 0.014956 0.015587
Eisosomes 0.000177 0.000279 0.000062 0.000040 0.000239 0.000161 0.000036 0.000032 0.000185 0.000170 0.000042 0.000035
Endoplasmic Reticulum 0.000447 0.001157 0.002241 0.001806 0.000383 0.001490 0.000917 0.001211 0.000438 0.001465 0.001252 0.001447
Endosome 0.007799 0.046323 0.002561 0.013108 0.027424 0.053185 0.001928 0.009139 0.010348 0.052663 0.002088 0.010711
Golgi 0.001056 0.014076 0.000126 0.007467 0.001383 0.027429 0.000067 0.007064 0.001099 0.026413 0.000082 0.007223
Lipid Particles 0.007787 0.023255 0.002814 0.000955 0.013694 0.015298 0.001019 0.001413 0.008554 0.015904 0.001474 0.001232
Mitochondria 0.005550 0.065870 0.001200 0.014173 0.003190 0.042983 0.000419 0.014855 0.005244 0.044724 0.000617 0.014585
Mitotic Spindle 0.001324 0.006507 0.007379 0.129698* 0.002092 0.048958 0.007581 0.102847* 0.001423 0.045728 0.007530 0.113479*
None 0.009505 0.005677 0.009417 0.006780 0.002355 0.007195 0.005882 0.005362 0.008577 0.007079 0.006777 0.005924
Nuclear Periphery 0.000329 0.000860 0.001383 0.001631 0.000549 0.001032 0.001141 0.001902 0.000358 0.001018 0.001202 0.001795
Nuclear Periphery Foci 0.000223 0.000987 0.000502 0.001775 0.000344 0.001804 0.000464 0.000668 0.000238 0.001742 0.000473 0.001106
Nucleolus 0.002465 0.005597 0.001727 0.001588 0.001627 0.004513 0.000485 0.000675 0.002356 0.004596 0.000800 0.001036
Nucleus 0.200233* 0.112351 0.738407* 0.253144* 0.183372* 0.069068 0.542237* 0.240713* 0.198043* 0.072361 0.591907* 0.245635*
Peroxisomes 0.004283 0.058744 0.001696 0.003775 0.010440 0.032241 0.000367 0.002355 0.005083 0.034258 0.000704 0.002917
Vacuole 0.035263 0.054795 0.027940 0.073237 0.047514 0.053410 0.039526 0.059048 0.036855 0.053515 0.036592 0.064666
Vacuole Periphery 0.000878 0.004837 0.000407 0.005456 0.001322 0.008126 0.000204 0.004036 0.000935 0.007876 0.000255 0.004598

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.36 -3.08 -7.18 1.15 -2.97 -7.59 -1.24 -5.26 0.77 -4.64 -8.07 -3.87 -8.87 0.55 -6.17
Bud Neck -2.16 4.24 -0.76 1.96 -4.74 -4.29 4.47 1.27 8.74 -8.93 -7.49 8.42 0.61 8.53 -10.33
Bud Site -2.86 -0.52 -10.97 -0.82 -9.45 -10.85 -1.91 -12.23 -0.54 -9.16 -11.26 -1.64 -16.05 -0.92 -12.36
Cell Periphery -0.71 0.71 1.11 1.99 0.60 -4.08 4.08 1.91 6.11 -2.51 -1.57 2.16 1.54 6.15 -1.93
Cytoplasm 13.47 26.08 17.80 -5.53 -12.32 2.53 1.66 -2.30 -8.50 -6.54 18.02 20.03 14.42 -7.38 -9.45
Cytoplasmic Foci -5.76 31.78 35.69 14.08 2.29 3.97 18.73 19.02 35.58 2.78 -5.25 38.43 39.99 37.88 3.38
Eisosomes -2.70 10.39 17.18 6.31 3.72 3.34 8.57 9.01 18.42 3.02 1.61 17.60 20.22 18.79 4.11
Endoplasmic Reticulum -1.61 -8.23 -14.99 -3.23 1.16 -2.49 -9.01 -10.46 -0.52 -1.77 -2.54 -11.59 -16.07 -1.56 -1.62
Endosome -4.25 6.34 1.41 4.40 -3.67 -5.05 5.82 5.17 16.91 -4.68 -15.07 9.92 5.43 17.32 -6.24
Golgi -3.97 3.67 -2.54 3.25 -3.95 -11.74 6.03 -2.11 10.41 -4.00 -12.24 4.59 -3.34 10.95 -5.52
Lipid Particles -2.24 6.07 10.06 3.23 4.85 -0.63 5.56 5.58 7.86 0.35 -3.95 10.42 11.26 8.50 3.00
Mitochondria -3.23 3.79 -1.09 3.14 -5.36 -11.06 3.09 -3.73 9.63 -7.62 -10.74 4.61 -1.94 10.26 -9.51
Mitotic Spindle -2.07 -1.58 -13.80 -11.93 -10.36 -8.28 -0.57 -11.93 -2.66 -13.68 -10.84 -2.73 -20.86 -4.46 -17.51
None 1.32 0.56 2.41 -0.07 0.67 -5.29 -3.37 -6.15 2.21 -0.73 0.74 2.02 2.39 1.97 -0.02
Nuclear Periphery -1.60 -13.22 -17.41 -4.99 -6.19 -1.45 -6.32 -10.25 -4.89 -6.41 -2.31 -16.33 -19.84 -5.52 -8.63
Nuclear Periphery Foci -2.00 -4.77 -2.77 -0.06 -1.49 -4.34 -3.23 -1.98 3.63 0.84 -4.90 -6.22 -3.76 2.73 -0.96
Nucleolus -1.35 1.59 2.11 1.85 0.59 -4.73 5.56 4.42 6.49 -4.05 -3.18 3.70 3.23 6.33 -1.57
Nucleus 4.47 -32.30 -15.05 -11.25 22.31 7.93 -20.80 -8.58 -35.12 19.35 21.81 -39.56 -19.40 -42.01 26.44
Peroxisomes -4.58 3.81 4.23 4.82 -0.45 -7.62 5.69 5.21 14.82 -4.53 -13.20 8.88 7.26 15.33 -3.15
Vacuole -2.42 -1.46 -18.51 -8.55 -13.30 -0.41 -2.53 -9.17 -12.89 -7.17 -5.30 -6.16 -24.65 -17.33 -12.46
Vacuole Periphery -3.10 2.99 -3.06 1.72 -4.10 -7.64 5.14 -1.51 6.64 -4.97 -8.51 5.90 -3.84 6.70 -6.56
Standard name
Human Ortholog
Description Minor sphingoid long-chain base kinase; possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules; LCB5 has a paralog, LCB4, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (20%), vacuole (2%), mixed (43%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Lcb5

Lcb5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lcb5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available