Standard name
Human Ortholog
Description Protein component of the synaptonemal complex axial elements; involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; promotes interhomolog recombination by phosphorylating Hop1p; also interacts with Mec3p and Ddc1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.99 0.98 0.96 0.96 0.96 0.89 0.79 0.81 0.98 0.99 0.99 0.57 0.59 0.57 0.89 0.91 0.77 0.85 0.76 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.11 0.06 0.28 0.33 0.21 0.08 0 0 0 0 0 0 0 0.07 0.06 0.09 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.21 0.18 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.28 0.28 0.3 0 0 0 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 1 1 3 0 3 3
Bud 0 1 0 0 1 2 2 2 4 2 4 0 0 0 0 0 0 1 1 7 0 6 7
Bud Neck 1 1 10 7 14 6 5 10 8 1 8 0 0 0 0 2 0 0 0 1 0 3 2
Bud Site 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0
Cell Periphery 3 2 1 2 2 0 4 3 1 0 0 0 1 2 1 1 0 1 1 5 0 3 3
Cytoplasm 189 302 492 332 503 328 455 362 258 142 208 114 331 321 232 198 169 174 280 338 57 252 154
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 0 0 2 0 20 12 17 0 0 3 0 5 4
Endosome 0 0 0 0 0 1 0 0 0 0 1 0 0 0 19 5 11 0 3 4 0 2 2
Golgi 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 3 0 0 0
Mitochondria 2 9 2 4 6 34 52 21 80 59 55 9 0 0 6 8 8 5 8 29 3 29 20
Nucleus 1 0 5 5 2 1 4 5 3 3 7 0 0 0 81 71 52 0 0 1 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 1 0 1 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 3 3 5 5 0 2 6 0 0 0 2 1 2 0 4 7 0 5 0
Vac/Vac Membrane 1 0 1 1 1 1 1 0 3 1 3 0 0 1 115 96 88 4 3 4 1 12 17
Unique Cell Count 193 307 498 336 511 340 474 376 289 180 258 116 333 323 410 338 297 196 308 438 68 333 222
Labelled Cell Count 197 316 515 351 532 376 529 410 358 211 296 123 335 325 478 396 348 196 308 438 68 333 222


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.0 4.8 4.3 4.9 3.8 4.2 4.5 3.4 3.1 3.6 4.1 4.2 4.3 6.4 6.6 6.1 4.0 3.9 4.4
Std Deviation (1e-4) 0.5 0.6 1.3 1.5 1.7 1.5 1.5 1.8 1.2 0.7 0.9 1.6 1.2 1.6 1.2 1.3 1.1 1.7 1.3 1.8
Intensity Change (Log2) -0.13 0.03 -0.32 -0.2 -0.09 -0.51 -0.64 -0.41 -0.21 -0.18 -0.14 0.42 0.46 0.35 -0.24 -0.29 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 12.5 12.5 12.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0128 1.5309 1.1406 0.5418 0.8498 1.1798 -0.3577 -0.4296 -0.3205 -0.1603 -0.5429 -0.7016 -1.1285 -1.2126 -1.3562 -1.1393 -1.199 -1.0311
Actin 0.0292 0.0002 0.0053 0.0001 0.0316 0.0034 0.0267 0.001 0.0066 0.0125 0.0033 0.0071 0.0458 0.0004 0.0025 0.0129 0.0215 0.0091
Bud 0.0028 0.011 0.0017 0 0.0014 0.0006 0.006 0.0061 0.0079 0.0031 0.0007 0.0038 0.0019 0.0024 0.0001 0.0012 0.0016 0.0014
Bud Neck 0.0088 0.0001 0.0011 0 0.025 0.0022 0.0032 0.0003 0.003 0.0019 0.0011 0.0026 0.0056 0.0008 0.0001 0.0002 0.0011 0.0011
Bud Periphery 0.0056 0.002 0.0028 0 0.0038 0.0017 0.0119 0.01 0.0159 0.0151 0.0009 0.007 0.0049 0.0036 0.0002 0.0029 0.0035 0.002
Bud Site 0.0133 0.0104 0.0112 0 0.0071 0.002 0.0088 0.0177 0.027 0.006 0.0126 0.0022 0.0036 0.0203 0.0003 0.0012 0.0024 0.0005
Cell Periphery 0.0007 0.0003 0.0009 0 0.0003 0.0002 0.0008 0.0006 0.0009 0.0003 0.0002 0.0002 0.0004 0.0004 0 0.0001 0.0002 0.0001
Cytoplasm 0.0266 0.0234 0.0455 0.0314 0.0283 0.0797 0.0847 0.1078 0.1005 0.1158 0.129 0.1572 0.0291 0.0541 0.0219 0.0208 0.0129 0.0388
Cytoplasmic Foci 0.0151 0.0016 0.0073 0.0049 0.0298 0.0133 0.0186 0.0049 0.014 0.0414 0.0145 0.0099 0.0104 0.0042 0.0046 0.0032 0.0062 0.007
Eisosomes 0.0016 0.0002 0.0006 0.0001 0.0004 0.0003 0.0004 0.0001 0.0003 0.0004 0.0001 0.0001 0.001 0.0001 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0024 0.0004 0.0021 0.0003 0.0017 0.0004 0.0049 0.0011 0.0022 0.0019 0.0026 0.0008 0.003 0.0004 0.0009 0.0009 0.0047 0.0011
Endosome 0.0142 0.0001 0.0037 0.0005 0.015 0.004 0.0172 0.001 0.0056 0.0416 0.0259 0.0019 0.0164 0.0005 0.0019 0.0041 0.006 0.0047
Golgi 0.0067 0 0.0022 0.0005 0.0078 0.0022 0.0054 0.0001 0.0013 0.018 0.0073 0.0007 0.0035 0.0001 0.0014 0.0013 0.0024 0.0013
Lipid Particles 0.0133 0.0001 0.0094 0.0074 0.0043 0.0095 0.0108 0.0002 0.0017 0.0131 0.0261 0.0006 0.0037 0.0002 0.0024 0.0013 0.0011 0.0012
Mitochondria 0.0211 0.0003 0.0083 0.0002 0.0369 0.0018 0.0057 0.0005 0.0064 0.0193 0.029 0.0028 0.0018 0.0008 0.0018 0.0042 0.0026 0.0058
None 0.8059 0.9484 0.8676 0.9267 0.7609 0.8596 0.7419 0.8407 0.7831 0.6816 0.7247 0.7941 0.8424 0.9012 0.9537 0.9326 0.8985 0.9129
Nuclear Periphery 0.0045 0.0002 0.0017 0.0001 0.0038 0.0016 0.0132 0.0007 0.0051 0.0019 0.0063 0.0007 0.0067 0.0007 0.0026 0.0052 0.019 0.0033
Nucleolus 0.0015 0.0001 0.0009 0.0001 0.0044 0.0009 0.0026 0.0004 0.0035 0.0013 0.0012 0.0004 0.0014 0.0008 0.0002 0.0006 0.0002 0.0004
Nucleus 0.0023 0.0004 0.002 0.0002 0.0235 0.0011 0.0143 0.0025 0.0043 0.0024 0.0027 0.0018 0.0048 0.0019 0.0006 0.002 0.0023 0.0021
Peroxisomes 0.0178 0.0001 0.0211 0.0269 0.0052 0.0088 0.0091 0.0003 0.0016 0.0069 0.0041 0.0014 0.0018 0.0003 0.0017 0.0015 0.0102 0.0014
Punctate Nuclear 0.0038 0.0004 0.0029 0.0003 0.0037 0.0056 0.0056 0.0025 0.0045 0.0105 0.0039 0.0037 0.0069 0.006 0.0024 0.0025 0.0008 0.0048
Vacuole 0.0018 0.0002 0.0011 0.0001 0.0034 0.001 0.0063 0.0015 0.0039 0.0039 0.0025 0.0006 0.0041 0.0005 0.0003 0.0007 0.0013 0.0006
Vacuole Periphery 0.001 0 0.0005 0 0.0017 0.0002 0.002 0.0002 0.0008 0.0009 0.0015 0.0001 0.0008 0.0001 0.0002 0.0006 0.0013 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.3326 0.3971 3.2413 4.0906 2.5917 3.5517 3.4127 5.1487 3.8899 4.5343
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein component of the synaptonemal complex axial elements; involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; promotes interhomolog recombination by phosphorylating Hop1p; also interacts with Mec3p and Ddc1p
Localization
Cell Percentages cytoplasm (23%), nucleus (7%), mixed (60%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Red1

Red1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Red1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available