Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0.06 0.08 0.05 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0.09 0.05 0 0.08 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.97 0.98 0.98 0.95 0.85 0.75 0.75 0.74 0.66 0.62 0.63 0.98 0.99 0.99 0.98 0.96 0.98 0.84 0.96 0.95 0.92 0.9 0.92
Endoplasmic Reticulum – 0 0 0 0 0.06 0.17 0.17 0.11 0.18 0.19 0.17 0 0 0 0 0 0 0.06 0 0 0 0.06 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0.12 0 0.13 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 1 2 12 16 8 17 14 31 0 0 0 1 0 0 0 0 0 0 0 1
Bud – 1 0 1 1 0 0 0 0 2 2 1 1 1 2 0 0 1 0 0 0 0 3 6
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 1 5 1 28 18 5 16 24 35 2 5 4 3 4 6 0 0 0 0 0 0
Cytoplasm – 103 47 130 201 97 223 273 92 139 163 201 191 405 447 363 357 417 22 114 67 275 405 437
Endoplasmic Reticulum – 2 1 1 7 7 51 63 14 39 50 55 5 4 7 13 16 14 1 1 1 9 25 18
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi – 0 1 0 1 1 13 15 5 11 18 23 0 0 0 2 2 1 0 0 0 1 0 0
Mitochondria – 0 0 0 0 5 0 6 15 4 34 33 0 0 0 1 1 0 0 0 0 2 2 0
Nucleus – 0 0 0 0 0 0 2 1 1 3 0 0 0 0 0 0 0 2 0 0 1 1 1
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 1 4 2 5 4 6 2 11 0 2 0 3 8 1 0 2 0 6 5 4
Unique Cell Count 106 48 133 212 114 299 366 124 211 262 320 195 411 453 372 370 425 27 120 71 300 451 477
Labelled Cell Count 106 49 133 217 117 329 398 144 235 310 391 199 417 460 386 388 441 27 120 71 300 451 477


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 92.8 76.2 79.0 60.3 31.5 36.5 32.0 23.9 22.7 20.7 19.0 84.8 88.3 85.9 85.3 82.9 93.8 75.2 81.7 85.1
Std Deviation (1e-4) 1.6 19.7 19.7 21.1 14.9 12.2 9.1 7.9 6.7 6.7 6.6 5.6 19.7 25.5 23.0 26.9 26.3 31.3 19.2 21.4 23.7
Intensity Change (Log2) – – – 0.05 -0.34 -1.28 -1.06 -1.25 -1.67 -1.75 -1.88 -2.01 0.15 0.21 0.17 0.16 0.12 0.3 -0.02 0.1 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 -0.9 -2.4 -3.6 -3.6 -3.5 -4.5 -4.9 -4.8 0 0.3 0.4 -0.1 -0.5 0.1
Endoplasmic Reticulum 0 0 0 2.7 2.7 0 2.8 2.9 2.7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.6 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 75.0408 95.9111 86.3187 69.9531 55.0693 73.4708 83.4307 141.6651 125.4177 102.9329 87.4047 87.4918 95.5086 127.6902 116.2305 117.8093 84.0568 122.4045
Actin 0.0021 0.0006 0.0019 0.0258 0.0045 0.0069 0.0247 0.0009 0.0253 0.0113 0.0018 0.026 0.0084 0.0002 0.0113 0.0001 0.0076 0.0016
Bud 0.0059 0.0075 0.0064 0.0037 0.0048 0.0033 0.0043 0.001 0.0027 0.0112 0.0133 0.0102 0.0025 0.0008 0.0016 0.0012 0.002 0.0034
Bud Neck 0.0008 0.0004 0.0013 0.0014 0.0011 0.0019 0.0009 0.0007 0.001 0.0012 0.0023 0.0022 0.0023 0.0003 0.0005 0.0004 0.0021 0.0013
Bud Periphery 0.0098 0.005 0.0056 0.003 0.0118 0.0021 0.0084 0.0006 0.0049 0.0239 0.0127 0.0118 0.003 0.0003 0.0006 0.0003 0.0009 0.001
Bud Site 0.0021 0.0026 0.0026 0.0139 0.0023 0.0008 0.0035 0.001 0.0078 0.0115 0.0014 0.0014 0.0094 0.0005 0.0009 0.0001 0.0053 0.0003
Cell Periphery 0.0222 0.0087 0.0185 0.0119 0.0081 0.0025 0.0362 0.0249 0.0333 0.0117 0.0149 0.0129 0.026 0.0044 0.003 0.0007 0.0021 0.003
Cytoplasm 0.6137 0.9072 0.7457 0.5863 0.507 0.6344 0.4731 0.8434 0.6994 0.5893 0.5077 0.5265 0.635 0.9375 0.8477 0.7987 0.6649 0.7904
Cytoplasmic Foci 0.0437 0.0012 0.023 0.0104 0.0989 0.0135 0.0212 0.0007 0.0135 0.0361 0.0253 0.0494 0.0096 0.001 0.0038 0.0018 0.0233 0.0065
Eisosomes 0.0003 0 0.0002 0.0008 0.0005 0.0001 0.0004 0.0001 0.001 0.0001 0.0003 0.0008 0.0008 0 0.0002 0 0.0002 0
Endoplasmic Reticulum 0.0288 0.0035 0.0038 0.0032 0.0072 0.0195 0.0155 0.0023 0.0061 0.0023 0.0091 0.0108 0.0354 0.0024 0.0038 0.0155 0.0047 0.009
Endosome 0.0125 0.0018 0.0088 0.0342 0.0365 0.0732 0.043 0.0015 0.0169 0.0357 0.0665 0.0076 0.0128 0.0017 0.0127 0.0094 0.0375 0.0145
Golgi 0.0022 0.0002 0.001 0.0234 0.0055 0.024 0.0095 0.0003 0.0062 0.0076 0.0156 0.008 0.0033 0.0002 0.001 0.0001 0.0084 0.0033
Lipid Particles 0.0092 0.0002 0.0048 0.0028 0.0122 0.0019 0.0113 0.0009 0.0028 0.0156 0.0115 0.0294 0.0036 0.0001 0.0012 0.0001 0.0078 0.0011
Mitochondria 0.01 0.0003 0.007 0.0257 0.0625 0.0279 0.0223 0.0076 0.0074 0.0053 0.0626 0.0226 0.0091 0.0002 0.0031 0.0002 0.0057 0.0068
None 0.0161 0.0003 0.0065 0.0025 0.0374 0.0054 0.0116 0.0002 0.0053 0.0017 0.0169 0.017 0.0164 0.0001 0.0025 0.0002 0.0071 0.0009
Nuclear Periphery 0.0164 0.0013 0.0036 0.0039 0.0071 0.0231 0.0318 0.0043 0.0149 0.0038 0.0118 0.0234 0.0252 0.0013 0.0115 0.0052 0.0175 0.0046
Nucleolus 0.0125 0.0002 0.0076 0.001 0.0238 0.0086 0.0086 0.0003 0.0009 0.0012 0.0084 0.0158 0.0022 0.0001 0.0003 0.0001 0.0006 0.0006
Nucleus 0.0135 0.0015 0.0049 0.0046 0.0108 0.0434 0.021 0.0028 0.0077 0.0055 0.0087 0.0112 0.0089 0.0014 0.0042 0.0027 0.0128 0.0043
Peroxisomes 0.001 0 0.0009 0.0018 0.002 0.0006 0.0031 0.0001 0.0005 0.0028 0.0028 0.0032 0.0025 0 0.0004 0 0.0343 0.0002
Punctate Nuclear 0.0036 0 0.0006 0.0004 0.0114 0.0015 0.0121 0 0.0017 0.0027 0.0039 0.0047 0.0037 0 0.001 0 0.0055 0.0001
Vacuole 0.1461 0.0529 0.124 0.2043 0.1167 0.0641 0.1996 0.0997 0.1268 0.1899 0.1587 0.1641 0.1487 0.0426 0.0713 0.1248 0.123 0.1082
Vacuole Periphery 0.0274 0.0043 0.0213 0.0348 0.0281 0.0413 0.0379 0.0069 0.0139 0.0296 0.0438 0.041 0.0312 0.0052 0.0172 0.0384 0.0266 0.0389

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1744.1836 1669.6472 2150.0552 1837.5404 2421.609 1700.1401 1579.0962 1786.2392 1902.3203 1675.2417
Translational Efficiency 1.6977 1.7238 1.3173 1.8069 1.5524 1.9042 1.6854 2.1092 1.5993 2.0545

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
349 599 2442 1887 1982 1731 1538 2684 2331 2330 3980 4571

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3073.83 5695.18 8889.82 8415.44 4273.57 5417.86 8450.54 9464.37 4093.94 5489.15 8720.07 9031.35
Standard Deviation 647.57 1192.17 1597.64 2038.02 910.19 1140.88 1587.72 1945.29 974.90 1160.63 1608.10 2050.21
Intensity Change Log 2 — 0.889704 1.532117 1.453001 — 0.342281 0.983602 1.147065 — 0.596948 1.238828 1.283026

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000160 0.000117 0.000068 0.000197 0.000052 0.000070 0.000118 0.000071 0.000068 0.000082 0.000087 0.000123
Bud Neck 0.000411 0.000786 0.000287 0.000593 0.000208 0.000261 0.000793 0.000211 0.000238 0.000396 0.000483 0.000369
Bud Site 0.006242 0.004288 0.002138 0.004625 0.001255 0.003663 0.002878 0.003465 0.002002 0.003824 0.002424 0.003944
Cell Periphery 0.033317 0.030856 0.028053 0.062528 0.009356 0.007994 0.042041 0.016808 0.012943 0.013871 0.033459 0.035682
Cytoplasm 0.227108* 0.624651* 0.771837* 0.531799* 0.734227* 0.811705* 0.570006* 0.758719* 0.658301* 0.763617* 0.693843* 0.665042*
Cytoplasmic Foci 0.031739 0.014578 0.004814 0.007002 0.010576 0.010951 0.008692 0.009722 0.013744 0.011883 0.006313 0.008599
Eisosomes 0.000003 0.000003 0.000002 0.000006 0.000001 0.000001 0.000003 0.000001 0.000001 0.000002 0.000002 0.000003
Endoplasmic Reticulum 0.004048 0.001190 0.001304 0.001774 0.001591 0.001088 0.001061 0.000746 0.001959 0.001114 0.001210 0.001170
Endosome 0.003037 0.001071 0.000381 0.000715 0.000681 0.000723 0.000632 0.000472 0.001034 0.000812 0.000478 0.000572
Golgi 0.319370* 0.092376 0.024426 0.053821 0.070971 0.052833 0.057209 0.040059 0.108162 0.062999 0.037094 0.045740
Lipid Particles 0.022345 0.024760 0.014397 0.027510 0.008883 0.008051 0.025414 0.010199 0.010899 0.012347 0.018654 0.017345
Mitochondria 0.000147 0.000166 0.000302 0.000516 0.000224 0.000335 0.000230 0.000412 0.000212 0.000292 0.000274 0.000455
Mitotic Spindle 0.002134 0.003099 0.000387 0.000780 0.000692 0.000927 0.000738 0.000548 0.000908 0.001485 0.000523 0.000644
None 0.001332 0.002281 0.001548 0.004237 0.002221 0.002820 0.002223 0.001861 0.002088 0.002682 0.001808 0.002842
Nuclear Periphery 0.000510 0.001119 0.000584 0.001022 0.000486 0.000286 0.001237 0.000335 0.000489 0.000500 0.000837 0.000618
Nuclear Periphery Foci 0.010647 0.017765 0.006272 0.010312 0.011130 0.008100 0.014565 0.005472 0.011057 0.010585 0.009476 0.007470
Nucleolus 0.000112 0.000191 0.000062 0.000312 0.000067 0.000160 0.000082 0.000070 0.000073 0.000168 0.000070 0.000170
Nucleus 0.005285 0.007250 0.005029 0.006641 0.003667 0.003396 0.008544 0.003092 0.003909 0.004387 0.006387 0.004557
Peroxisomes 0.000038 0.000035 0.000065 0.000098 0.000013 0.000030 0.000021 0.000048 0.000016 0.000031 0.000048 0.000069
Vacuole 0.061601 0.089899 0.090000 0.184484 0.047736 0.035431 0.180710 0.080035 0.049812 0.049434 0.125053 0.123154
Vacuole Periphery 0.270413* 0.083519 0.048044 0.101031* 0.095964 0.051176 0.082804 0.067652 0.122083* 0.059491 0.061476 0.081432

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.99 2.09 -1.11 -3.34 -5.46 -1.29 -4.01 -1.40 -0.29 2.67 -1.08 -1.81 -3.93 -3.36 -2.24
Bud Neck -3.31 0.93 -2.65 1.41 -4.09 -1.43 -9.27 -0.64 1.01 9.15 -3.93 -6.32 -4.66 0.15 2.79
Bud Site 1.56 3.59 1.25 -0.63 -5.60 -5.35 -5.30 -6.75 0.38 -1.06 -4.67 -2.02 -6.40 -0.31 -4.91
Cell Periphery 0.62 1.54 -7.78 -9.63 -13.29 1.69 -16.29 -6.76 -8.03 11.74 -0.92 -16.11 -17.25 -15.96 -1.29
Cytoplasm -18.78 -32.84 -17.28 5.57 22.94 -7.85 14.44 -2.42 6.28 -17.74 -10.54 -3.39 -0.51 12.24 3.63
Cytoplasmic Foci 4.92 8.71 7.76 5.06 -3.21 -0.51 1.64 1.01 1.52 -0.72 1.75 9.23 5.99 4.53 -4.18
Eisosomes 0.19 2.98 -3.87 -4.16 -7.26 -0.03 -8.91 -1.36 -1.43 8.06 -1.21 -5.13 -7.10 -6.14 -2.20
Endoplasmic Reticulum 3.77 3.66 3.03 -2.80 -2.61 2.31 2.29 4.24 2.89 2.00 3.91 3.51 3.81 -0.45 0.37
Endosome 7.23 10.27 8.79 3.54 -5.62 -0.76 0.77 4.31 4.68 3.77 3.03 9.36 7.44 4.83 -2.80
Golgi 17.45 24.35 21.78 7.40 -13.33 5.09 4.43 10.48 4.60 6.93 11.14 20.16 17.52 7.00 -5.59
Lipid Particles -0.74 2.63 -1.67 -0.99 -8.13 0.77 -12.16 -1.53 -2.37 11.73 -1.39 -7.85 -6.44 -4.78 1.53
Mitochondria -0.66 -5.35 -6.03 -5.69 -3.42 -1.80 -0.26 -4.01 -1.14 -2.47 -1.66 -1.86 -5.98 -3.13 -4.19
Mitotic Spindle -0.67 6.87 4.87 1.61 -4.07 -2.54 -0.92 1.86 3.79 2.70 -1.57 6.00 3.16 2.24 -2.14
None -4.85 -0.56 -3.41 -2.29 -2.91 -0.84 0.00 0.78 1.33 0.64 -1.07 0.70 -1.64 -0.30 -2.20
Nuclear Periphery -3.89 -1.54 -7.10 0.57 -6.46 4.83 -12.25 3.69 -1.97 16.86 -0.24 -8.36 -3.32 -2.46 5.68
Nuclear Periphery Foci -4.96 5.51 0.27 5.29 -5.81 3.54 -3.89 8.51 4.04 12.15 0.55 2.33 5.88 4.95 4.54
Nucleolus -0.83 1.35 -1.80 -0.89 -2.36 -0.79 -1.16 -0.16 0.77 1.12 -1.05 0.25 -2.10 -0.05 -2.25
Nucleus -0.69 0.05 -0.70 0.11 -2.66 0.39 -6.52 0.41 0.04 8.37 -0.69 -4.08 -1.52 -1.05 3.95
Peroxisomes 0.28 -2.91 -3.05 -3.20 -1.72 -1.30 -2.56 -6.63 -1.25 -4.97 -1.46 -7.53 -6.26 -2.87 -2.35
Vacuole -3.78 -5.00 -17.72 -12.59 -16.43 4.03 -23.67 -9.31 -13.28 17.11 0.01 -21.63 -22.03 -21.68 0.72
Vacuole Periphery 13.68 17.42 13.10 -2.79 -14.82 10.69 3.22 7.13 -5.13 4.21 14.44 15.17 10.03 -7.52 -8.46
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rps30a

Rps30a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps30a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available