Standard name | |
---|---|
Human Ortholog | |
Description | Catalytic subunit of 1,3-beta-D-glucan synthase; functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling; FKS1 has a paralog, GSC2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 2 | 0 | 1 | 0 | – | 9 | 4 | 4 | 1 | 3 | 2 | 3 | 2 | 0 | 0 | 0 | 8 | 4 | 1 | 2 | 11 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 21 | 21 | 7 | 64 | 56 | 88 | – | 248 | 189 | 256 | 137 | 296 | 175 | 420 | 618 | 25 | 48 | 48 | 4 | 5 | 4 | 7 | 14 | 10 |
Endoplasmic Reticulum | 3 | 0 | 1 | 0 | 8 | 4 | – | 9 | 3 | 1 | 1 | 4 | 8 | 11 | 2 | 7 | 4 | 8 | 2 | 0 | 0 | 1 | 3 | 2 |
Endosome | 15 | 3 | 0 | 6 | 13 | 1 | – | 4 | 0 | 2 | 0 | 0 | 12 | 30 | 7 | 9 | 15 | 9 | 9 | 4 | 2 | 11 | 16 | 33 |
Golgi | 0 | 0 | 0 | 0 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 5 | 0 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 19 | 0 | 20 | 76 | 52 | 74 | – | 174 | 123 | 112 | 73 | 126 | 14 | 2 | 0 | 6 | 7 | 4 | 3 | 1 | 2 | 3 | 4 | 5 |
Nucleus | 11 | 5 | 7 | 50 | 75 | 80 | – | 179 | 75 | 102 | 41 | 50 | 11 | 18 | 2 | 39 | 27 | 48 | 2 | 1 | 4 | 2 | 5 | 16 |
Nuclear Periphery | 2 | 1 | 0 | 2 | 4 | 0 | – | 3 | 3 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 3 | 6 |
Nucleolus | 0 | 0 | 0 | 3 | 0 | 0 | – | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 0 | 2 | 0 | 0 | 1 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 1 | – | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 3 | 0 | 1 | 1 | 4 | 4 |
Vac/Vac Membrane | 140 | 60 | 39 | 203 | 413 | 265 | – | 584 | 319 | 357 | 184 | 270 | 195 | 246 | 159 | 108 | 105 | 116 | 159 | 74 | 67 | 56 | 258 | 431 |
Unique Cell Count | 182 | 80 | 61 | 322 | 525 | 401 | 931 | 522 | 609 | 321 | 556 | 381 | 639 | 747 | 166 | 180 | 195 | 198 | 97 | 85 | 88 | 332 | 528 | |
Labelled Cell Count | 211 | 90 | 76 | 405 | 626 | 514 | 1215 | 717 | 839 | 438 | 750 | 421 | 731 | 790 | 195 | 215 | 243 | 198 | 97 | 85 | 88 | 332 | 528 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 7.4 | 5.3 | 6.0 | 8.2 | 7.1 | – | 6.6 | 5.9 | 5.8 | 5.5 | 5.3 | 6.4 | 6.1 | 5.4 | 8.9 | 9.5 | 9.2 | 7.3 | 7.5 | 7.6 |
Std Deviation (1e-4) | 2.4 | 2.1 | 1.6 | 2.0 | 2.5 | 3.0 | – | 2.8 | 2.8 | 2.6 | 2.5 | 2.5 | 2.0 | 1.4 | 1.2 | 2.6 | 3.2 | 2.7 | 2.4 | 2.5 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.16 | 0.62 | 0.42 | – | 0.3 | 0.15 | 0.12 | 0.05 | 0.0 | 0.26 | 0.19 | 0.01 | 0.74 | 0.83 | 0.78 | 0.46 | 0.5 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | -0.2 | 1.9 | – | 2.6 | 3.9 | 4.7 | 4.6 | 6.2 | 5.1 | 8.3 | 12.8 | 0.7 | 2.4 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.5 | -5.2 | -2.6 | – | -2.7 | -1.6 | -2.7 | -1.7 | -1.8 | 0 | 0 | 0 | -6.1 | -6.2 | -7.2 |
Nucleus | 0.8 | 0.6 | 1.6 | – | 1.5 | 0.6 | 1.1 | 0.3 | -0.6 | 0 | 0 | 0 | 2.0 | 0.7 | 2.2 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.1 | 2.6 | 0.3 | – | -0.2 | -0.4 | -0.8 | -1.0 | -2.3 | -1.9 | -3.9 | -7.4 | 0.2 | -0.8 | -0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5138 | 8.3122 | 6.6171 | 5.9039 | 5.4512 | 6.5516 | 2.2787 | 8.9059 | 7.5526 | 5.8052 | 4.8155 | 6.204 | 4.2021 | 9.0912 | 9.8737 | 8.3177 | 8.3353 | 9.0068 |
Actin | 0.0067 | 0 | 0.0021 | 0.0002 | 0.0097 | 0.0028 | 0.0063 | 0.0001 | 0.0117 | 0.0315 | 0.0048 | 0 | 0.0108 | 0.0102 | 0.0001 | 0 | 0 | 0 |
Bud | 0.0029 | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0004 | 0.0007 | 0.0003 | 0.0008 | 0.0002 | 0.0002 | 0.0008 | 0.0005 | 0.0004 | 0.0004 | 0.0004 | 0.0003 |
Bud Neck | 0.001 | 0.0012 | 0.0013 | 0.0007 | 0.0013 | 0.003 | 0.001 | 0.0008 | 0.0006 | 0.0023 | 0.0012 | 0.0015 | 0.0012 | 0.0019 | 0.001 | 0.0037 | 0.0011 | 0.0014 |
Bud Periphery | 0.0018 | 0.0011 | 0.0011 | 0.0004 | 0.001 | 0.001 | 0.0008 | 0.0016 | 0.0006 | 0.0013 | 0.0004 | 0.0007 | 0.0016 | 0.0011 | 0.0009 | 0.0011 | 0.0003 | 0.0008 |
Bud Site | 0.002 | 0.0019 | 0.0013 | 0.001 | 0.001 | 0.0005 | 0.0017 | 0.0008 | 0.0011 | 0.0084 | 0.0009 | 0.0007 | 0.0061 | 0.0033 | 0.0015 | 0.002 | 0.0029 | 0.0005 |
Cell Periphery | 0.0017 | 0.0041 | 0.0031 | 0.0018 | 0.0018 | 0.004 | 0.0024 | 0.005 | 0.0025 | 0.0036 | 0.0011 | 0.0037 | 0.0018 | 0.0041 | 0.0025 | 0.0043 | 0.0014 | 0.0035 |
Cytoplasm | 0.0083 | 0.0029 | 0.0044 | 0.0009 | 0.0012 | 0.001 | 0.0026 | 0.0039 | 0.0144 | 0.0022 | 0.008 | 0.0057 | 0.0046 | 0.007 | 0.0078 | 0.0011 | 0.0033 | 0.0032 |
Cytoplasmic Foci | 0.0188 | 0.013 | 0.0069 | 0.0038 | 0.0105 | 0.0027 | 0.0103 | 0.0029 | 0.0139 | 0.0202 | 0.0328 | 0.0057 | 0.0189 | 0.0091 | 0.0077 | 0.0047 | 0.0114 | 0.0036 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0.0004 | 0.0003 | 0 | 0.0002 | 0.0003 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0052 | 0.0053 | 0.0092 | 0.002 | 0.0037 | 0.0032 | 0.0034 | 0.0076 | 0.0096 | 0.0043 | 0.0035 | 0.0062 | 0.0057 | 0.0037 | 0.0099 | 0.0018 | 0.0007 | 0.0067 |
Endosome | 0.2734 | 0.1569 | 0.2036 | 0.2123 | 0.2889 | 0.125 | 0.3396 | 0.1438 | 0.3381 | 0.2679 | 0.3812 | 0.2101 | 0.3197 | 0.1313 | 0.2923 | 0.1084 | 0.2966 | 0.192 |
Golgi | 0.0172 | 0.0052 | 0.0096 | 0.012 | 0.0147 | 0.0083 | 0.0185 | 0.0068 | 0.0209 | 0.0145 | 0.0315 | 0.0108 | 0.0235 | 0.006 | 0.0157 | 0.0039 | 0.0079 | 0.0099 |
Lipid Particles | 0.0309 | 0.0137 | 0.0108 | 0.0176 | 0.0519 | 0.0204 | 0.0334 | 0.0152 | 0.0205 | 0.0102 | 0.0523 | 0.0254 | 0.0378 | 0.0216 | 0.021 | 0.0244 | 0.0113 | 0.0498 |
Mitochondria | 0.0485 | 0.0095 | 0.0159 | 0.0054 | 0.0486 | 0.0103 | 0.0176 | 0.0038 | 0.0065 | 0.0056 | 0.0082 | 0.0049 | 0.0352 | 0.028 | 0.0062 | 0.0026 | 0.0005 | 0.0066 |
None | 0.0019 | 0.0001 | 0.0003 | 0.0001 | 0.0012 | 0.0001 | 0.0004 | 0.0001 | 0.0012 | 0.0011 | 0.0266 | 0.0002 | 0.001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.0135 | 0.006 | 0.0178 | 0.0035 | 0.0301 | 0.0155 | 0.0055 | 0.0125 | 0.01 | 0.0037 | 0.0322 | 0.0074 | 0.0217 | 0.0182 | 0.0071 | 0.0033 | 0.0011 | 0.0343 |
Nucleolus | 0.0048 | 0.005 | 0.0032 | 0.0015 | 0.0058 | 0.005 | 0.0088 | 0.0026 | 0.0013 | 0.0074 | 0.0021 | 0.0025 | 0.0029 | 0.0057 | 0.0021 | 0.081 | 0.0031 | 0.0055 |
Nucleus | 0.007 | 0.004 | 0.0097 | 0.0012 | 0.0034 | 0.0062 | 0.0046 | 0.0025 | 0.0016 | 0.0032 | 0.0041 | 0.0028 | 0.0038 | 0.0028 | 0.0027 | 0.0124 | 0.0017 | 0.0231 |
Peroxisomes | 0.003 | 0.0009 | 0.0008 | 0.0005 | 0.0047 | 0.0005 | 0.0016 | 0.0002 | 0.003 | 0.0018 | 0.0052 | 0.0003 | 0.0059 | 0.0027 | 0.0004 | 0.0016 | 0.0007 | 0.0006 |
Punctate Nuclear | 0.0015 | 0.0001 | 0.0003 | 0.0002 | 0.0029 | 0.0002 | 0.0005 | 0.0001 | 0.0009 | 0.0015 | 0.0218 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0002 |
Vacuole | 0.3693 | 0.5788 | 0.4938 | 0.5591 | 0.3849 | 0.5453 | 0.4018 | 0.5768 | 0.3951 | 0.4745 | 0.3111 | 0.5313 | 0.3336 | 0.5661 | 0.4728 | 0.5954 | 0.5523 | 0.4466 |
Vacuole Periphery | 0.1803 | 0.1896 | 0.2041 | 0.1755 | 0.132 | 0.2446 | 0.1388 | 0.2125 | 0.1459 | 0.1336 | 0.0706 | 0.1796 | 0.1625 | 0.1759 | 0.1476 | 0.147 | 0.103 | 0.2112 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 483.2171 | 459.1177 | 288.3021 | 288.8552 | 440.4131 | 360.7434 | 402.9323 | 292.6923 | 236.4283 | 460.8302 |
Translational Efficiency | 0.8251 | 0.7702 | 0.6771 | 0.5813 | 0.6054 | 0.8943 | 0.6781 | 0.5038 | 0.4765 | 0.527 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
64 | 620 | 482 | 1066 | 355 | 610 | 1840 | 1285 | 419 | 1230 | 2322 | 2351 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 769.59 | 923.75 | 1009.64 | 1160.11 | 795.11 | 949.06 | 1102.83 | 1080.90 | 791.21 | 936.30 | 1083.49 | 1116.82 |
Standard Deviation | 135.59 | 196.29 | 118.95 | 205.01 | 201.33 | 159.30 | 185.47 | 184.35 | 192.96 | 179.35 | 177.83 | 197.95 |
Intensity Change Log 2 | — | 0.263412 | 0.391679 | 0.592100 | — | 0.255345 | 0.471984 | 0.443007 | — | 0.259318 | 0.433045 | 0.518263 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000749 | 0.000148 | 0.001259 | 0.009511 | 0.000075 | 0.000041 | 0.004514 | 0.007103 | 0.000178 | 0.000095 | 0.003838 | 0.008195 |
Bud Neck | 0.010187 | 0.009481 | 0.033459 | 0.024949 | 0.030680 | 0.012353 | 0.021923 | 0.017908 | 0.027550 | 0.010905 | 0.024318 | 0.021100 |
Bud Site | 0.014478 | 0.027733 | 0.031799 | 0.078241 | 0.024125 | 0.011134 | 0.052098 | 0.069119 | 0.022651 | 0.019501 | 0.047884 | 0.073255 |
Cell Periphery | 0.000409 | 0.000910 | 0.000277 | 0.012761 | 0.001402 | 0.001169 | 0.005096 | 0.004689 | 0.001250 | 0.001038 | 0.004095 | 0.008349 |
Cytoplasm | 0.320740* | 0.039106 | 0.111107* | 0.141773* | 0.044813 | 0.015959 | 0.096648 | 0.166758* | 0.086959 | 0.027627 | 0.099650 | 0.155429* |
Cytoplasmic Foci | 0.145919 | 0.035139 | 0.063337 | 0.033355 | 0.030888 | 0.015223 | 0.043203 | 0.067531 | 0.048458 | 0.025262 | 0.047383 | 0.052035 |
Eisosomes | 0.000278 | 0.000022 | 0.000071 | 0.000821 | 0.000069 | 0.000034 | 0.000270 | 0.000386 | 0.000101 | 0.000028 | 0.000228 | 0.000583 |
Endoplasmic Reticulum | 0.002838 | 0.003401 | 0.012219 | 0.099317 | 0.005468 | 0.006475 | 0.122702* | 0.072259 | 0.005066 | 0.004925 | 0.099768 | 0.084527 |
Endosome | 0.022332 | 0.151068 | 0.151687 | 0.063054 | 0.144654 | 0.141656 | 0.165860 | 0.106635 | 0.125970 | 0.146400 | 0.162918 | 0.086874 |
Golgi | 0.000726 | 0.011360 | 0.001625 | 0.006541 | 0.005257 | 0.003693 | 0.011425 | 0.008258 | 0.004565 | 0.007558 | 0.009391 | 0.007479 |
Lipid Particles | 0.019049 | 0.002996 | 0.013447 | 0.075863 | 0.005607 | 0.002433 | 0.075433 | 0.050776 | 0.007660 | 0.002717 | 0.062566 | 0.062151 |
Mitochondria | 0.000245 | 0.002213 | 0.000407 | 0.001067 | 0.005280 | 0.003218 | 0.001455 | 0.003992 | 0.004511 | 0.002711 | 0.001237 | 0.002666 |
Mitotic Spindle | 0.000084 | 0.000253 | 0.006032 | 0.000752 | 0.000251 | 0.000111 | 0.000820 | 0.003801 | 0.000226 | 0.000183 | 0.001902 | 0.002419 |
None | 0.000359 | 0.000459 | 0.000562 | 0.000087 | 0.004050 | 0.000088 | 0.000120 | 0.000231 | 0.003486 | 0.000275 | 0.000211 | 0.000166 |
Nuclear Periphery | 0.000612 | 0.000214 | 0.002868 | 0.000184 | 0.000829 | 0.001354 | 0.000319 | 0.000337 | 0.000796 | 0.000780 | 0.000848 | 0.000267 |
Nuclear Periphery Foci | 0.001540 | 0.001049 | 0.020096 | 0.007727 | 0.001099 | 0.001098 | 0.009665 | 0.007968 | 0.001167 | 0.001073 | 0.011830 | 0.007859 |
Nucleolus | 0.000371 | 0.000800 | 0.001787 | 0.000153 | 0.003824 | 0.000768 | 0.000262 | 0.000232 | 0.003296 | 0.000784 | 0.000579 | 0.000196 |
Nucleus | 0.088597 | 0.004026 | 0.053325 | 0.005404 | 0.034997 | 0.005435 | 0.005097 | 0.006576 | 0.043184 | 0.004725 | 0.015108 | 0.006045 |
Peroxisomes | 0.003106 | 0.001784 | 0.007066 | 0.003273 | 0.002051 | 0.001073 | 0.002123 | 0.005077 | 0.002213 | 0.001431 | 0.003149 | 0.004259 |
Vacuole | 0.366963* | 0.590276* | 0.484760* | 0.429893* | 0.562934* | 0.684079* | 0.375434* | 0.395697* | 0.533001* | 0.636796* | 0.398128* | 0.411202* |
Vacuole Periphery | 0.000418 | 0.117560* | 0.002811 | 0.005275 | 0.091647 | 0.092608 | 0.005535 | 0.004669 | 0.077712 | 0.105186* | 0.004970 | 0.004943 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.16 | -0.65 | -6.44 | -7.39 | -6.29 | 1.47 | -8.70 | -7.95 | -8.00 | -2.29 | 0.98 | -8.66 | -10.62 | -10.78 | -4.87 |
Bud Neck | 0.03 | -4.74 | -3.66 | -7.24 | 2.35 | 3.77 | 1.90 | 2.82 | -2.23 | 2.33 | 4.11 | 0.87 | 1.75 | -6.30 | 2.20 |
Bud Site | -1.41 | -1.49 | -6.29 | -9.51 | -8.52 | 3.57 | -6.96 | -8.52 | -13.38 | -3.09 | 0.88 | -6.71 | -11.62 | -15.65 | -6.60 |
Cell Periphery | -2.68 | 1.63 | -9.98 | -9.46 | -10.13 | 0.85 | -7.36 | -5.00 | -5.43 | 0.75 | 0.99 | -7.01 | -10.34 | -10.84 | -5.43 |
Cytoplasm | 8.48 | 5.62 | 5.06 | -13.94 | -1.98 | 4.44 | -7.01 | -13.74 | -23.06 | -9.37 | 6.56 | -1.97 | -7.47 | -25.60 | -9.36 |
Cytoplasmic Foci | 5.74 | 4.27 | 5.88 | 0.57 | 7.23 | 3.89 | -2.86 | -7.75 | -15.51 | -7.41 | 4.66 | 0.31 | -0.64 | -10.50 | -2.01 |
Eisosomes | 5.24 | 4.18 | -1.65 | -2.51 | -2.34 | 2.93 | -8.47 | -8.23 | -9.25 | -2.51 | 6.12 | -6.13 | -3.28 | -3.82 | -2.37 |
Endoplasmic Reticulum | -0.54 | -5.05 | -14.96 | -15.04 | -13.27 | -0.53 | -21.42 | -12.59 | -12.78 | 7.42 | 0.08 | -21.15 | -18.68 | -19.72 | 3.16 |
Endosome | -15.33 | -11.73 | -7.95 | 10.97 | 7.59 | 0.19 | -1.65 | 3.68 | 4.35 | 9.63 | -2.01 | -3.07 | 4.44 | 10.24 | 15.05 |
Golgi | -6.52 | -1.15 | -7.71 | 3.04 | -6.16 | 0.85 | -3.17 | -1.40 | -4.29 | 3.29 | -1.77 | -2.90 | -1.64 | 0.49 | 2.71 |
Lipid Particles | 1.92 | 0.68 | -5.87 | -14.19 | -12.13 | 2.55 | -20.19 | -11.87 | -13.38 | 5.49 | 2.86 | -17.81 | -15.86 | -19.35 | 0.56 |
Mitochondria | -5.38 | -1.78 | -2.09 | 2.39 | -1.59 | 1.24 | 2.68 | 0.80 | -0.34 | -1.76 | 1.39 | 2.70 | 1.43 | 0.24 | -1.70 |
Mitotic Spindle | -3.27 | -2.75 | -2.97 | -2.29 | 2.42 | 1.14 | -0.78 | -3.59 | -4.17 | -3.38 | 0.92 | -2.51 | -3.55 | -4.46 | -1.33 |
None | -0.38 | -0.83 | 2.93 | 1.55 | 2.07 | 1.76 | 1.74 | 1.69 | -1.48 | -1.11 | 1.68 | 1.71 | 1.74 | 0.83 | 0.69 |
Nuclear Periphery | 2.72 | -7.43 | 2.92 | 0.61 | 9.37 | -0.92 | 1.98 | 1.86 | 1.96 | -0.70 | 0.08 | -0.87 | 2.42 | 1.96 | 8.63 |
Nuclear Periphery Foci | 0.68 | -7.46 | -8.89 | -9.96 | 5.09 | 0.04 | -10.80 | -7.12 | -7.17 | 1.41 | 0.23 | -12.98 | -10.86 | -11.05 | 4.30 |
Nucleolus | -2.23 | -4.52 | 2.81 | 3.84 | 5.14 | 2.30 | 2.69 | 2.71 | 4.33 | 1.00 | 2.23 | 2.31 | 2.76 | 5.59 | 5.39 |
Nucleus | 4.56 | 1.42 | 4.46 | -1.73 | 9.40 | 4.33 | 4.39 | 4.18 | -1.00 | -1.67 | 5.99 | 3.95 | 5.74 | -1.92 | 7.46 |
Peroxisomes | 1.10 | -2.14 | 0.04 | -2.67 | 3.43 | 2.28 | -0.11 | -4.46 | -7.58 | -5.47 | 1.81 | -1.66 | -3.71 | -7.36 | -2.96 |
Vacuole | -5.35 | -2.78 | -1.63 | 10.39 | 3.17 | -6.24 | 10.09 | 8.25 | 21.08 | -2.41 | -5.91 | 7.80 | 6.60 | 21.78 | -2.23 |
Vacuole Periphery | -20.21* | -8.74 | -8.83 | 19.35 | -4.65 | -0.16 | 11.21 | 11.32 | 18.79 | 1.76 | -3.64 | 10.87 | 10.87 | 26.73 | 0.24 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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