Standard name | |
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Human Ortholog | |
Description | Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 3 | 1 | 2 | 0 | 9 | – | 0 | 3 | 3 | 4 | 0 | 0 | 1 | 3 | 3 | 1 | 2 | 2 | 1 | 1 | 1 | 2 |
Bud | 1 | 1 | 1 | 3 | 1 | 6 | 15 | – | 9 | 21 | 22 | 13 | 3 | 3 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 27 | 34 | 26 | 17 | 46 | 43 | 64 | – | 49 | 43 | 34 | 57 | 26 | 40 | 67 | 18 | 25 | 16 | 1 | 0 | 1 | 1 | 2 | 1 |
Endoplasmic Reticulum | 9 | 7 | 4 | 2 | 3 | 9 | 29 | – | 12 | 8 | 10 | 7 | 2 | 15 | 8 | 1 | 5 | 7 | 3 | 0 | 3 | 2 | 5 | 4 |
Endosome | 68 | 40 | 88 | 21 | 65 | 40 | 24 | – | 16 | 2 | 3 | 14 | 59 | 108 | 88 | 19 | 54 | 29 | 127 | 118 | 126 | 113 | 153 | 162 |
Golgi | 3 | 1 | 2 | 0 | 1 | 0 | 3 | – | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 16 | 6 | 7 | 5 | 4 | 2 | 3 | 7 |
Mitochondria | 19 | 24 | 4 | 21 | 14 | 70 | 129 | – | 161 | 133 | 167 | 124 | 40 | 12 | 17 | 9 | 3 | 5 | 9 | 11 | 11 | 8 | 9 | 11 |
Nucleus | 3 | 1 | 1 | 1 | 4 | 3 | 4 | – | 2 | 7 | 1 | 2 | 0 | 1 | 4 | 3 | 1 | 1 | 1 | 1 | 0 | 1 | 2 | 1 |
Nuclear Periphery | 6 | 6 | 2 | 0 | 1 | 2 | 3 | – | 7 | 3 | 2 | 1 | 2 | 1 | 1 | 3 | 0 | 0 | 14 | 6 | 10 | 11 | 20 | 15 |
Nucleolus | 11 | 7 | 3 | 5 | 5 | 4 | 1 | – | 1 | 3 | 1 | 0 | 2 | 10 | 5 | 5 | 9 | 8 | 1 | 0 | 0 | 0 | 1 | 1 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
SpindlePole | 5 | 3 | 10 | 9 | 9 | 2 | 3 | – | 0 | 2 | 4 | 5 | 1 | 6 | 5 | 0 | 5 | 4 | 3 | 1 | 2 | 1 | 3 | 3 |
Vac/Vac Membrane | 117 | 182 | 152 | 33 | 111 | 66 | 67 | – | 44 | 42 | 35 | 35 | 171 | 231 | 207 | 95 | 154 | 96 | 68 | 151 | 93 | 94 | 126 | 78 |
Unique Cell Count | 217 | 258 | 230 | 87 | 210 | 197 | 279 | 246 | 205 | 214 | 208 | 249 | 320 | 333 | 130 | 202 | 126 | 246 | 305 | 261 | 242 | 336 | 297 | |
Labelled Cell Count | 269 | 306 | 297 | 113 | 262 | 245 | 352 | 304 | 269 | 285 | 264 | 306 | 430 | 405 | 159 | 275 | 173 | 246 | 305 | 261 | 242 | 336 | 297 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.9 | 7.1 | 7.5 | 6.2 | 7.0 | 5.9 | 5.8 | – | 5.4 | 4.9 | 4.9 | 5.4 | 6.3 | 6.8 | 7.0 | 10.8 | 11.9 | 12.0 | 6.2 | 6.5 | 6.9 |
Std Deviation (1e-4) | 1.4 | 1.3 | 1.6 | 1.5 | 1.5 | 1.4 | 1.3 | – | 1.2 | 1.0 | 1.1 | 1.1 | 1.0 | 1.3 | 1.3 | 2.5 | 2.7 | 2.5 | 1.3 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.28 | -0.1 | -0.34 | -0.37 | – | -0.46 | -0.62 | -0.61 | -0.46 | -0.25 | -0.13 | -0.09 | 0.53 | 0.67 | 0.68 | -0.27 | -0.21 | -0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 1.4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 3.2 | – | 0 | 0 | 4.7 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.9 | 3.0 | 2.9 | 3.4 | – | 2.6 | 2.8 | 1.4 | 4.3 | -0.3 | 0.4 | 2.8 | 0.7 | 0.3 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 1.7 | 3.9 | – | 1.9 | 0 | 1.8 | 1.1 | 0 | 1.9 | 0 | 0 | 0 | 0 |
Endosome | -2.4 | -1.6 | -4.0 | -8.0 | – | -8.4 | 0 | -9.6 | -7.8 | -3.5 | -1.1 | -3.0 | -4.7 | -2.5 | -2.9 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 |
Mitochondria | 6.6 | 2.6 | 9.2 | 11.4 | – | 14.6 | 0 | 16.5 | 13.3 | 5.4 | 1.4 | 2.1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 | 2.0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2.0 | 0 | -2.1 | -2.3 | – | 0 | 0 | -1.5 | -1.1 | -2.9 | -1.7 | -2.1 | 0 | -1.1 | 0 |
Vacuole | -4.5 | -2.8 | -6.7 | -9.5 | – | -10.7 | -9.6 | -10.6 | -10.4 | 0.6 | 1.5 | -1.0 | 1.4 | 2.3 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.1435 | 4.6287 | 4.5996 | 3.7414 | 4.0252 | 4.2936 | 2.5715 | 2.9904 | 3.0112 | 2.54 | 2.9657 | 2.5813 | 2.334 | 2.1971 | 2.5192 | 2.8047 | 1.9759 | 2.3294 |
Actin | 0.0134 | 0.0003 | 0.0058 | 0.0001 | 0.0033 | 0.0015 | 0.0122 | 0.0001 | 0.0142 | 0 | 0.0187 | 0.0006 | 0.0125 | 0.0003 | 0.0062 | 0.0232 | 0 | 0.0115 |
Bud | 0.0017 | 0.0044 | 0.0009 | 0.0004 | 0.0026 | 0.0022 | 0.0005 | 0.0009 | 0.0015 | 0.0001 | 0.0045 | 0.0005 | 0.0004 | 0.0004 | 0.0009 | 0.0004 | 0.0001 | 0.0004 |
Bud Neck | 0.0022 | 0.0003 | 0.0012 | 0.0027 | 0.0002 | 0.0015 | 0.0007 | 0.0005 | 0.0005 | 0.0001 | 0.0008 | 0.0005 | 0.0013 | 0.0003 | 0.0037 | 0.0009 | 0.0001 | 0.001 |
Bud Periphery | 0.0017 | 0.007 | 0.0016 | 0.0008 | 0.0068 | 0.0058 | 0.0008 | 0.0012 | 0.0018 | 0.0001 | 0.0041 | 0.0014 | 0.0004 | 0.0002 | 0.0008 | 0.0005 | 0.0001 | 0.0008 |
Bud Site | 0.0021 | 0.0022 | 0.0077 | 0.0005 | 0.0006 | 0.001 | 0.0014 | 0.0015 | 0.002 | 0.0001 | 0.0086 | 0.0003 | 0.0018 | 0.0019 | 0.0025 | 0.001 | 0.0001 | 0.0006 |
Cell Periphery | 0.0008 | 0.0003 | 0.0005 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0002 |
Cytoplasm | 0.0185 | 0.0475 | 0.0195 | 0.0047 | 0.0078 | 0.0172 | 0.0079 | 0.0292 | 0.0305 | 0.0037 | 0.0078 | 0.0061 | 0.014 | 0.0232 | 0.0245 | 0.0065 | 0.0028 | 0.0144 |
Cytoplasmic Foci | 0.0438 | 0.0289 | 0.0361 | 0.0237 | 0.0273 | 0.0323 | 0.0442 | 0.0366 | 0.0391 | 0.0087 | 0.045 | 0.0311 | 0.0483 | 0.0232 | 0.0466 | 0.0164 | 0.0132 | 0.0249 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.004 | 0.0041 | 0.0059 | 0.0019 | 0.0009 | 0.0055 | 0.0062 | 0.0046 | 0.0031 | 0.0033 | 0.0023 | 0.0033 | 0.0046 | 0.0046 | 0.0023 | 0.0012 | 0.001 | 0.0038 |
Endosome | 0.534 | 0.5797 | 0.5424 | 0.5444 | 0.6175 | 0.5546 | 0.5691 | 0.5662 | 0.5847 | 0.5795 | 0.6095 | 0.6102 | 0.4897 | 0.5547 | 0.5414 | 0.5967 | 0.6267 | 0.5326 |
Golgi | 0.0276 | 0.0208 | 0.0403 | 0.0186 | 0.0559 | 0.0323 | 0.0392 | 0.027 | 0.0417 | 0.0108 | 0.0858 | 0.0283 | 0.0217 | 0.0135 | 0.0335 | 0.0256 | 0.0236 | 0.0282 |
Lipid Particles | 0.0432 | 0.0072 | 0.0282 | 0.0057 | 0.0483 | 0.006 | 0.0383 | 0.0087 | 0.0103 | 0.0024 | 0.0145 | 0.014 | 0.0234 | 0.0049 | 0.0172 | 0.0047 | 0.0012 | 0.0081 |
Mitochondria | 0.023 | 0.0101 | 0.0386 | 0.0124 | 0.0195 | 0.0344 | 0.0164 | 0.0075 | 0.025 | 0.004 | 0.0363 | 0.0176 | 0.0133 | 0.0019 | 0.0126 | 0.006 | 0.0013 | 0.007 |
None | 0.0095 | 0.0118 | 0.0197 | 0.0006 | 0.0002 | 0.003 | 0.0028 | 0.0012 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0222 | 0.0015 | 0.0063 | 0.0003 | 0.0001 | 0.0007 |
Nuclear Periphery | 0.0152 | 0.0118 | 0.0089 | 0.0048 | 0.0024 | 0.0104 | 0.0174 | 0.0087 | 0.0041 | 0.0131 | 0.0025 | 0.0067 | 0.0121 | 0.0107 | 0.01 | 0.0042 | 0.0023 | 0.0118 |
Nucleolus | 0.0035 | 0.0006 | 0.0009 | 0.0005 | 0.0001 | 0.0007 | 0.0007 | 0.0007 | 0.001 | 0.0001 | 0.0003 | 0.0012 | 0.003 | 0.0003 | 0.0018 | 0.0004 | 0 | 0.001 |
Nucleus | 0.0046 | 0.0021 | 0.0031 | 0.0008 | 0.0006 | 0.0025 | 0.0018 | 0.0012 | 0.0013 | 0.0006 | 0.0009 | 0.0012 | 0.0026 | 0.0013 | 0.0021 | 0.0006 | 0.0002 | 0.002 |
Peroxisomes | 0.011 | 0.001 | 0.0049 | 0.0007 | 0.0075 | 0.0021 | 0.0178 | 0.0011 | 0.003 | 0.0001 | 0.0074 | 0.0016 | 0.0129 | 0.0012 | 0.0065 | 0.0045 | 0.0002 | 0.0011 |
Punctate Nuclear | 0.0016 | 0.0003 | 0.0011 | 0.0001 | 0.0001 | 0.0006 | 0.003 | 0.0002 | 0.0045 | 0 | 0.0007 | 0.0004 | 0.0042 | 0.0002 | 0.0026 | 0.0011 | 0 | 0.0028 |
Vacuole | 0.1718 | 0.1923 | 0.1668 | 0.2418 | 0.1382 | 0.1823 | 0.1592 | 0.2102 | 0.1593 | 0.2167 | 0.0979 | 0.1795 | 0.2225 | 0.2515 | 0.1907 | 0.1786 | 0.1776 | 0.2185 |
Vacuole Periphery | 0.0664 | 0.0674 | 0.0656 | 0.1344 | 0.06 | 0.104 | 0.0601 | 0.0921 | 0.0717 | 0.1564 | 0.0516 | 0.0951 | 0.0886 | 0.1042 | 0.0873 | 0.1271 | 0.1494 | 0.1287 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.3451 | 14.1817 | 12.7389 | 18.1159 | 25.711 | 20.4453 | 18.5843 | 14.1349 | 17.6149 | 30.9404 |
Translational Efficiency | 1.2075 | 1.1925 | 0.8767 | 0.8241 | 1.0008 | 1.6144 | 0.7799 | 0.7989 | 0.8856 | 0.9322 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1883 | 1536 | 354 | 202 | 2050 | 1448 | 273 | 207 | 3933 | 2984 | 627 | 409 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 785.04 | 829.99 | 1199.62 | 1141.25 | 841.93 | 904.78 | 1131.57 | 1026.70 | 814.69 | 866.28 | 1169.99 | 1083.27 |
Standard Deviation | 93.72 | 121.07 | 144.48 | 158.92 | 103.68 | 619.47 | 160.36 | 183.72 | 103.03 | 441.76 | 155.30 | 181.21 |
Intensity Change Log 2 | — | 0.080328 | 0.611739 | 0.539777 | — | 0.103867 | 0.426554 | 0.286242 | — | 0.092557 | 0.518879 | 0.414144 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000134 | 0.000138 | 0.000315 | 0.000763 | 0.000052 | 0.000207 | 0.000213 | 0.000340 | 0.000091 | 0.000171 | 0.000271 | 0.000549 |
Bud Neck | 0.003463 | 0.002721 | 0.017428 | 0.012189 | 0.001982 | 0.003767 | 0.016313 | 0.008511 | 0.002691 | 0.003228 | 0.016943 | 0.010327 |
Bud Site | 0.009471 | 0.011866 | 0.014344 | 0.064234 | 0.005642 | 0.011083 | 0.021746 | 0.034013 | 0.007475 | 0.011486 | 0.017567 | 0.048939 |
Cell Periphery | 0.000136 | 0.000098 | 0.000094 | 0.000109 | 0.000092 | 0.000190 | 0.000089 | 0.000116 | 0.000113 | 0.000143 | 0.000091 | 0.000112 |
Cytoplasm | 0.006844 | 0.012724 | 0.002121 | 0.020211 | 0.006829 | 0.016433 | 0.011862 | 0.012939 | 0.006836 | 0.014524 | 0.006362 | 0.016531 |
Cytoplasmic Foci | 0.075188 | 0.112361 | 0.028908 | 0.053510 | 0.056251 | 0.089055 | 0.032852 | 0.039110 | 0.065317 | 0.101052 | 0.030625 | 0.046222 |
Eisosomes | 0.000032 | 0.000019 | 0.000037 | 0.000013 | 0.000015 | 0.000024 | 0.000027 | 0.000020 | 0.000023 | 0.000021 | 0.000033 | 0.000017 |
Endoplasmic Reticulum | 0.001154 | 0.001449 | 0.002575 | 0.000581 | 0.001174 | 0.000962 | 0.001121 | 0.001678 | 0.001164 | 0.001213 | 0.001942 | 0.001136 |
Endosome | 0.502114* | 0.413967* | 0.213275* | 0.192930 | 0.351234* | 0.478408* | 0.179144 | 0.230027* | 0.423471* | 0.445237* | 0.198414 | 0.211705* |
Golgi | 0.090515 | 0.071466 | 0.023757 | 0.039263 | 0.052798 | 0.068565 | 0.020724 | 0.025922 | 0.070856 | 0.070058 | 0.022436 | 0.032511 |
Lipid Particles | 0.004214 | 0.002989 | 0.003097 | 0.000998 | 0.002932 | 0.006434 | 0.001557 | 0.001950 | 0.003546 | 0.004661 | 0.002427 | 0.001480 |
Mitochondria | 0.057987 | 0.020923 | 0.002370 | 0.003040 | 0.028660 | 0.031213 | 0.003658 | 0.004839 | 0.042701 | 0.025916 | 0.002931 | 0.003950 |
Mitotic Spindle | 0.001420 | 0.008484 | 0.030109 | 0.071850 | 0.002145 | 0.008089 | 0.038537 | 0.013604 | 0.001798 | 0.008292 | 0.033778 | 0.042371 |
None | 0.000147 | 0.000189 | 0.000085 | 0.000117 | 0.000116 | 0.000922 | 0.000226 | 0.000107 | 0.000131 | 0.000545 | 0.000147 | 0.000112 |
Nuclear Periphery | 0.000150 | 0.000474 | 0.011448 | 0.001741 | 0.000498 | 0.000381 | 0.004729 | 0.002565 | 0.000331 | 0.000429 | 0.008523 | 0.002158 |
Nuclear Periphery Foci | 0.000827 | 0.003207 | 0.027880 | 0.004188 | 0.001335 | 0.001697 | 0.007012 | 0.003582 | 0.001092 | 0.002474 | 0.018794 | 0.003882 |
Nucleolus | 0.000264 | 0.000497 | 0.019031 | 0.001238 | 0.000488 | 0.000827 | 0.015907 | 0.005256 | 0.000380 | 0.000657 | 0.017671 | 0.003272 |
Nucleus | 0.000228 | 0.000576 | 0.014790 | 0.001613 | 0.000437 | 0.004340 | 0.013592 | 0.004730 | 0.000337 | 0.002403 | 0.014268 | 0.003190 |
Peroxisomes | 0.003274 | 0.003858 | 0.009232 | 0.007747 | 0.001510 | 0.004539 | 0.006178 | 0.005378 | 0.002354 | 0.004189 | 0.007902 | 0.006548 |
Vacuole | 0.132291 | 0.192674 | 0.429242* | 0.409122* | 0.275005* | 0.161878 | 0.469611* | 0.482753* | 0.206678* | 0.177730 | 0.446819* | 0.446387* |
Vacuole Periphery | 0.110147* | 0.139319* | 0.149863* | 0.114545* | 0.210808* | 0.110988* | 0.154904* | 0.122560* | 0.162614* | 0.125571* | 0.152058* | 0.118602* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.13 | -2.65 | -1.42 | -1.42 | -1.02 | -1.93 | -3.31 | -1.71 | -0.73 | -0.55 | -1.86 | -4.15 | -1.90 | -1.59 | -1.12 |
Bud Neck | 1.12 | -6.26 | -4.49 | -4.85 | 1.80 | -2.70 | -8.17 | -6.31 | -4.07 | 4.37 | -1.20 | -9.73 | -6.83 | -6.22 | 3.92 |
Bud Site | -1.73 | -2.50 | -5.71 | -5.47 | -5.06 | -4.10 | -6.70 | -4.58 | -3.64 | -1.69 | -4.23 | -6.51 | -7.22 | -6.51 | -5.20 |
Cell Periphery | 2.17 | 2.30 | 0.90 | -1.06 | -1.16 | -3.40 | -0.19 | -1.26 | 2.20 | -1.21 | -1.95 | 1.58 | -0.37 | 1.46 | -1.66 |
Cytoplasm | -4.03 | 5.40 | -4.62 | -2.90 | -5.89 | -4.52 | -2.15 | -2.49 | 0.90 | 0.25 | -5.99 | -0.66 | -5.14 | -1.56 | -3.85 |
Cytoplasmic Foci | -9.63 | 13.66 | 3.31 | 8.65 | -3.98 | -9.19 | 5.58 | 3.50 | 8.98 | -0.92 | -13.54 | 13.28 | 4.69 | 12.48 | -3.60 |
Eisosomes | 3.52 | -1.42 | 4.88 | 2.70 | 6.25 | -2.24 | -4.21 | -1.74 | 0.91 | 2.40 | 0.72 | -3.75 | 2.74 | 1.83 | 6.17 |
Endoplasmic Reticulum | -0.84 | -4.08 | 2.78 | 2.37 | 5.44 | 1.02 | -0.72 | -1.40 | -1.87 | -0.91 | -0.25 | -4.06 | 0.05 | 0.23 | 3.47 |
Endosome | 9.54 | 23.05 | 21.80 | 15.46 | 1.29 | -13.05 | 14.82 | 8.05 | 15.52 | -3.04 | -3.08 | 25.70 | 20.26 | 21.67 | -1.11 |
Golgi | 4.55 | 15.00 | 8.44 | 5.47 | -2.13 | -4.26 | 8.86 | 5.19 | 7.75 | -0.97 | 0.34 | 16.61 | 9.63 | 9.24 | -2.17 |
Lipid Particles | 1.62 | 1.13 | 3.97 | 3.73 | 2.97 | -3.02 | 1.84 | 1.40 | 3.98 | -0.38 | -1.66 | 1.75 | 3.77 | 5.05 | 2.03 |
Mitochondria | 11.43 | 19.80 | 18.62 | 9.34 | -0.54 | -0.89 | 13.58 | 11.50 | 10.70 | -0.80 | 7.96 | 23.95 | 21.63 | 14.12 | -1.08 |
Mitotic Spindle | -5.40 | -5.87 | -5.60 | -5.08 | -3.13 | -5.29 | -5.57 | -3.27 | -1.58 | 3.57 | -7.48 | -8.06 | -6.10 | -5.19 | -1.07 |
None | -1.05 | 1.63 | 0.38 | 1.35 | -0.94 | -3.63 | -3.08 | 0.37 | 3.68 | 3.32 | -3.77 | -1.45 | 0.50 | 3.87 | 1.86 |
Nuclear Periphery | -4.26 | -6.27 | -3.52 | -2.81 | 5.16 | 1.40 | -2.91 | -1.57 | -1.66 | 1.30 | -1.71 | -6.75 | -2.65 | -2.51 | 4.59 |
Nuclear Periphery Foci | -2.52 | -6.05 | -2.83 | -1.07 | 4.95 | -0.85 | -3.50 | -1.33 | -1.06 | 1.79 | -2.59 | -6.74 | -2.84 | -1.47 | 5.23 |
Nucleolus | -1.81 | -6.49 | -2.63 | -2.11 | 6.03 | -1.10 | -4.82 | -2.77 | -2.49 | 3.23 | -1.58 | -8.04 | -3.35 | -2.97 | 6.38 |
Nucleus | -2.54 | -4.99 | -2.80 | -2.33 | 4.36 | -4.06 | -4.47 | -3.82 | 0.18 | 3.20 | -4.33 | -6.66 | -4.72 | -0.99 | 5.34 |
Peroxisomes | -0.92 | -4.87 | -2.64 | -2.31 | 0.53 | -5.14 | -4.23 | -3.28 | -0.59 | 0.66 | -4.27 | -6.64 | -4.01 | -2.25 | 0.97 |
Vacuole | -10.02 | -22.48 | -14.47 | -11.07 | 1.03 | 16.95 | -12.35 | -11.37 | -17.52 | -1.07 | 6.22 | -23.46 | -17.81 | -19.94 | -0.23 |
Vacuole Periphery | -5.29 | -3.74 | 0.24 | 2.90 | 2.80 | 17.24* | 6.08 | 8.20 | -0.90 | 2.00 | 9.07 | 2.40 | 6.33 | 1.46 | 3.35 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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