Standard name | |
---|---|
Human Ortholog | |
Description | Cytoplasmic 60S subunit biogenesis factor; associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 0 | 0 | 2 | 4 | 5 | 4 | 0 | 6 | 3 | 7 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud | 2 | 1 | 3 | 2 | 3 | 9 | 36 | 10 | 15 | 24 | 11 | 20 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 1 | 28 | 8 | 19 | 23 | 24 | 39 | 32 | 35 | 35 | 40 | 12 | 24 | 7 | 12 | 16 | 6 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 221 | 340 | 606 | 374 | 414 | 517 | 538 | 565 | 389 | 449 | 365 | 383 | 447 | 665 | 555 | 289 | 361 | 345 | 223 | 326 | 609 | 437 | 526 | 493 |
Endoplasmic Reticulum | 1 | 2 | 10 | 3 | 9 | 12 | 7 | 10 | 15 | 8 | 5 | 11 | 2 | 15 | 12 | 8 | 12 | 7 | 0 | 1 | 17 | 0 | 2 | 6 |
Endosome | 1 | 0 | 1 | 0 | 0 | 1 | 5 | 4 | 3 | 2 | 0 | 3 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 3 | 1 | 3 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Mitochondria | 2 | 2 | 5 | 13 | 48 | 162 | 235 | 158 | 138 | 248 | 223 | 261 | 0 | 0 | 0 | 2 | 3 | 1 | 1 | 1 | 3 | 1 | 1 | 2 |
Nucleus | 1 | 1 | 1 | 1 | 2 | 2 | 3 | 1 | 0 | 3 | 0 | 3 | 0 | 2 | 1 | 7 | 3 | 5 | 1 | 0 | 1 | 1 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 4 |
Nucleolus | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 2 | 0 | 18 | 7 | 19 | 24 | 53 | 51 | 25 | 42 | 32 | 34 | 0 | 4 | 0 | 11 | 10 | 12 | 3 | 2 | 7 | 2 | 4 | 2 |
Unique Cell Count | 230 | 344 | 633 | 390 | 477 | 643 | 741 | 723 | 522 | 654 | 561 | 606 | 450 | 677 | 565 | 308 | 379 | 354 | 235 | 339 | 657 | 457 | 546 | 526 |
Labelled Cell Count | 237 | 349 | 674 | 408 | 516 | 754 | 909 | 842 | 621 | 818 | 675 | 763 | 464 | 713 | 576 | 332 | 406 | 377 | 235 | 339 | 657 | 457 | 546 | 526 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.9 | 12.6 | 12.6 | 11.4 | 10.4 | 8.5 | 7.7 | 8.0 | 6.8 | 6.7 | 6.2 | 6.1 | 14.7 | 14.6 | 13.8 | 17.5 | 16.0 | 15.4 | 14.7 | 14.9 | 16.1 |
Std Deviation (1e-4) | 2.0 | 2.3 | 2.1 | 1.7 | 1.6 | 1.8 | 1.6 | 1.7 | 1.3 | 1.5 | 1.6 | 1.5 | 2.3 | 2.0 | 2.0 | 4.9 | 3.6 | 4.0 | 2.6 | 2.7 | 3.3 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.27 | -0.56 | -0.71 | -0.65 | -0.88 | -0.91 | -1.02 | -1.04 | 0.23 | 0.22 | 0.14 | 0.48 | 0.35 | 0.3 | 0.22 | 0.25 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1.7 | 4.9 | 1.7 | 3.3 | 4.0 | 2.4 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.0 | -0.4 | -0.8 | -1.1 | 0.8 | 1.3 | 0.8 | 1.4 | 1.7 | -1.5 | -0.8 | -3.3 | -0.4 | -0.2 | -2.3 |
Cytoplasm | 0.1 | -5.4 | -8.4 | -11.4 | -9.4 | -10.4 | -12.6 | -13.6 | -14.3 | 3.6 | 2.7 | 2.5 | -1.3 | -0.4 | 1.4 |
Endoplasmic Reticulum | 0 | 0.4 | 0.4 | -1.1 | -0.3 | 1.5 | -0.5 | -1.1 | 0.3 | 0 | 0.8 | 0.7 | 1.1 | 1.7 | 0.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.0 | 7.2 | 12.9 | 15.0 | 11.9 | 13.2 | 16.8 | 17.1 | 18.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.1 | 1.0 | 0.9 | 3.6 | 3.5 | 1.7 | 3.0 | 2.5 | 2.4 | -3.6 | -3.2 | -4.0 | 0.6 | -0.2 | 0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.8698 | 13.3069 | 12.7785 | 12.2312 | 11.725 | 12.9753 | 7.1523 | 11.9897 | 11.1002 | 11.2567 | 9.8522 | 11.2095 | 9.9754 | 13.0727 | 11.9885 | 11.467 | 10.9115 | 11.7253 |
Actin | 0.0096 | 0.0085 | 0.0022 | 0.0001 | 0.0004 | 0.0011 | 0.0251 | 0.0074 | 0.0011 | 0.0025 | 0.0006 | 0.0027 | 0.0153 | 0.0006 | 0.0103 | 0.0021 | 0.0054 | 0.0022 |
Bud | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.0002 | 0.0012 | 0.0012 | 0.0002 | 0.0063 | 0.0002 | 0.0024 | 0.0014 | 0.0036 | 0.0044 | 0.004 | 0.0097 | 0.0017 |
Bud Neck | 0.0009 | 0.0002 | 0.0005 | 0.0003 | 0.0005 | 0.0008 | 0.0047 | 0.0001 | 0.0004 | 0.0007 | 0.0003 | 0.0009 | 0.0006 | 0.0005 | 0.0016 | 0.0005 | 0.0012 | 0.0017 |
Bud Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0002 | 0.0003 | 0.0027 | 0.0001 | 0.001 | 0.001 | 0.0005 | 0.0017 | 0.0011 | 0.0057 | 0.0007 |
Bud Site | 0.0008 | 0.0009 | 0.0131 | 0.0002 | 0.0002 | 0.0001 | 0.023 | 0.0007 | 0.0002 | 0.0039 | 0.0002 | 0.0004 | 0.0031 | 0.0015 | 0.0052 | 0.0014 | 0.0089 | 0.0021 |
Cell Periphery | 0.0005 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0001 |
Cytoplasm | 0.7538 | 0.9532 | 0.908 | 0.9279 | 0.9549 | 0.9131 | 0.6293 | 0.9733 | 0.9604 | 0.8664 | 0.8381 | 0.9041 | 0.8405 | 0.9602 | 0.9168 | 0.9139 | 0.9057 | 0.9259 |
Cytoplasmic Foci | 0.0309 | 0.0062 | 0.0133 | 0.027 | 0.0096 | 0.0089 | 0.0479 | 0.0042 | 0.0064 | 0.0185 | 0.0127 | 0.0129 | 0.0257 | 0.0064 | 0.0115 | 0.0087 | 0.0156 | 0.0105 |
Eisosomes | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0753 | 0.0038 | 0.023 | 0.0054 | 0.0031 | 0.0422 | 0.0335 | 0.0025 | 0.0026 | 0.0031 | 0.0013 | 0.0213 | 0.0204 | 0.0058 | 0.0073 | 0.0048 | 0.0044 | 0.0164 |
Endosome | 0.0252 | 0.0084 | 0.0182 | 0.026 | 0.0202 | 0.0225 | 0.0532 | 0.0027 | 0.0078 | 0.0416 | 0.0662 | 0.0266 | 0.0292 | 0.0114 | 0.0134 | 0.0298 | 0.0193 | 0.0222 |
Golgi | 0.0113 | 0.0024 | 0.0029 | 0.0011 | 0.0023 | 0.0028 | 0.0096 | 0.0011 | 0.0006 | 0.0008 | 0.0203 | 0.0035 | 0.0081 | 0.0015 | 0.0036 | 0.0065 | 0.0071 | 0.0039 |
Lipid Particles | 0.0021 | 0.0027 | 0.0039 | 0.0003 | 0 | 0.0001 | 0.0249 | 0.0001 | 0.0001 | 0.0006 | 0.0171 | 0.0001 | 0.0081 | 0.0002 | 0.0034 | 0.0003 | 0.0003 | 0.0002 |
Mitochondria | 0.0027 | 0.0021 | 0.0004 | 0.0003 | 0.001 | 0.0018 | 0.008 | 0.0004 | 0.0145 | 0.0007 | 0.0178 | 0.0034 | 0.0102 | 0.0002 | 0.0042 | 0.0045 | 0.0039 | 0.006 |
None | 0.0081 | 0.0017 | 0.0009 | 0.0019 | 0.0007 | 0.0009 | 0.0135 | 0.0019 | 0.0007 | 0.0025 | 0.0004 | 0.0053 | 0.0053 | 0.0008 | 0.0016 | 0.0015 | 0.0014 | 0.0006 |
Nuclear Periphery | 0.0222 | 0.0008 | 0.0022 | 0.0009 | 0.0007 | 0.0012 | 0.0385 | 0.0005 | 0.0007 | 0.0014 | 0.0011 | 0.0022 | 0.0065 | 0.001 | 0.0019 | 0.0021 | 0.001 | 0.0013 |
Nucleolus | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0077 | 0 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0014 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0 |
Nucleus | 0.0442 | 0.0012 | 0.0013 | 0.0011 | 0.0013 | 0.0011 | 0.0289 | 0.0007 | 0.0007 | 0.0019 | 0.0011 | 0.0073 | 0.0029 | 0.002 | 0.0021 | 0.0021 | 0.0025 | 0.0009 |
Peroxisomes | 0.0011 | 0.0026 | 0.0032 | 0.0001 | 0.0001 | 0 | 0.0257 | 0.001 | 0.0002 | 0.0003 | 0.0046 | 0.0002 | 0.0047 | 0.0001 | 0.0041 | 0.0001 | 0.0006 | 0.0002 |
Punctate Nuclear | 0.0073 | 0.0005 | 0.0017 | 0.0007 | 0.0001 | 0.0001 | 0.0142 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0012 | 0.0056 | 0.0005 | 0.0013 | 0.0003 | 0.0007 | 0.0001 |
Vacuole | 0.0026 | 0.0036 | 0.0036 | 0.0051 | 0.003 | 0.0021 | 0.0059 | 0.0014 | 0.0021 | 0.0355 | 0.0125 | 0.003 | 0.0068 | 0.0024 | 0.0039 | 0.0139 | 0.0045 | 0.002 |
Vacuole Periphery | 0.0007 | 0.0004 | 0.0007 | 0.001 | 0.0011 | 0.0008 | 0.0028 | 0.0002 | 0.0005 | 0.0096 | 0.0053 | 0.0011 | 0.0029 | 0.0003 | 0.0009 | 0.0021 | 0.0011 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 48.842 | 37.9668 | 43.9498 | 48.3064 | 34.1409 | 52.6353 | 50.0965 | 58.2962 | 49.2292 | 45.4758 |
Translational Efficiency | 1.9992 | 2.2992 | 1.6319 | 1.6347 | 2.014 | 2.0826 | 1.7706 | 1.3511 | 1.6194 | 1.4554 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1175 | 1321 | 1729 | 1538 | 2057 | 2072 | 756 | 1300 | 3232 | 3393 | 2485 | 2838 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1095.35 | 1418.12 | 1380.35 | 1408.41 | 1157.47 | 1321.16 | 1358.50 | 1432.73 | 1134.89 | 1358.91 | 1373.70 | 1419.55 |
Standard Deviation | 176.77 | 235.86 | 187.57 | 193.57 | 168.84 | 197.17 | 192.91 | 239.11 | 174.35 | 218.25 | 189.48 | 215.97 |
Intensity Change Log 2 | — | 0.372588 | 0.333642 | 0.362675 | — | 0.190830 | 0.231040 | 0.307792 | — | 0.282065 | 0.281838 | 0.334738 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000104 | 0.000865 | 0.000227 | 0.000345 | 0.000153 | 0.000252 | 0.000275 | 0.000873 | 0.000135 | 0.000491 | 0.000241 | 0.000587 |
Bud Neck | 0.000669 | 0.000334 | 0.000695 | 0.001179 | 0.000297 | 0.000266 | 0.001824 | 0.001244 | 0.000432 | 0.000292 | 0.001039 | 0.001209 |
Bud Site | 0.010990 | 0.011323 | 0.007624 | 0.015570 | 0.011170 | 0.010389 | 0.013149 | 0.020296 | 0.011104 | 0.010753 | 0.009305 | 0.017734 |
Cell Periphery | 0.000112 | 0.000185 | 0.000117 | 0.000210 | 0.000132 | 0.000107 | 0.000118 | 0.000574 | 0.000125 | 0.000138 | 0.000118 | 0.000377 |
Cytoplasm | 0.866247* | 0.864275* | 0.910729* | 0.804922* | 0.862727* | 0.890104* | 0.645988* | 0.893869* | 0.864007* | 0.880048* | 0.830188* | 0.845666* |
Cytoplasmic Foci | 0.076076 | 0.075612 | 0.030692 | 0.032367 | 0.086489 | 0.060881 | 0.066669 | 0.026878 | 0.082703 | 0.066616 | 0.041637 | 0.029852 |
Eisosomes | 0.000006 | 0.000004 | 0.000003 | 0.000004 | 0.000004 | 0.000003 | 0.000014 | 0.000005 | 0.000005 | 0.000003 | 0.000006 | 0.000004 |
Endoplasmic Reticulum | 0.001329 | 0.001946 | 0.000394 | 0.000519 | 0.002751 | 0.000911 | 0.001310 | 0.000162 | 0.002234 | 0.001314 | 0.000672 | 0.000355 |
Endosome | 0.002719 | 0.004749 | 0.001995 | 0.004849 | 0.003158 | 0.003454 | 0.009700 | 0.001967 | 0.002998 | 0.003958 | 0.004339 | 0.003529 |
Golgi | 0.013340 | 0.008935 | 0.001122 | 0.003442 | 0.010061 | 0.010061 | 0.000615 | 0.003393 | 0.011253 | 0.009623 | 0.000968 | 0.003420 |
Lipid Particles | 0.000681 | 0.001555 | 0.001484 | 0.002316 | 0.000773 | 0.001042 | 0.002165 | 0.003155 | 0.000739 | 0.001242 | 0.001691 | 0.002701 |
Mitochondria | 0.000673 | 0.000903 | 0.000034 | 0.000204 | 0.000827 | 0.000534 | 0.000036 | 0.000146 | 0.000771 | 0.000678 | 0.000034 | 0.000177 |
Mitotic Spindle | 0.000932 | 0.003457 | 0.001474 | 0.005715 | 0.000697 | 0.002975 | 0.001172 | 0.002990 | 0.000783 | 0.003163 | 0.001382 | 0.004466 |
None | 0.000854 | 0.001238 | 0.000750 | 0.000181 | 0.000775 | 0.000625 | 0.000297 | 0.000390 | 0.000804 | 0.000864 | 0.000612 | 0.000277 |
Nuclear Periphery | 0.000010 | 0.000035 | 0.000042 | 0.000059 | 0.000013 | 0.000012 | 0.000169 | 0.000021 | 0.000011 | 0.000021 | 0.000080 | 0.000042 |
Nuclear Periphery Foci | 0.000231 | 0.001224 | 0.002182 | 0.001452 | 0.000326 | 0.000492 | 0.004714 | 0.001688 | 0.000291 | 0.000777 | 0.002952 | 0.001560 |
Nucleolus | 0.000068 | 0.000087 | 0.000062 | 0.000112 | 0.000033 | 0.000030 | 0.000468 | 0.000081 | 0.000046 | 0.000052 | 0.000186 | 0.000097 |
Nucleus | 0.004232 | 0.003361 | 0.011925 | 0.005528 | 0.002448 | 0.002729 | 0.015017 | 0.004876 | 0.003096 | 0.002975 | 0.012866 | 0.005229 |
Peroxisomes | 0.000387 | 0.000346 | 0.000179 | 0.000524 | 0.000201 | 0.000226 | 0.000513 | 0.000866 | 0.000268 | 0.000273 | 0.000281 | 0.000680 |
Vacuole | 0.015177 | 0.014940 | 0.027900 | 0.117179 | 0.010904 | 0.011694 | 0.234687* | 0.035529 | 0.012457 | 0.012958 | 0.090810 | 0.079778 |
Vacuole Periphery | 0.005163 | 0.004625 | 0.000371 | 0.003325 | 0.006063 | 0.003212 | 0.001101 | 0.000997 | 0.005736 | 0.003762 | 0.000593 | 0.002259 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.60 | -5.23 | -7.22 | 1.08 | -2.41 | -1.98 | -4.48 | -2.98 | -2.55 | -2.45 | -1.89 | -5.59 | -3.99 | -0.53 | -2.88 |
Bud Neck | 4.40 | -1.98 | -6.44 | -11.96 | -5.31 | 0.76 | -14.33 | -5.84 | -6.02 | 2.31 | 3.74 | -12.45 | -9.23 | -10.88 | -1.90 |
Bud Site | -0.36 | 3.23 | -3.81 | -3.23 | -6.18 | 0.62 | -2.13 | -5.18 | -5.65 | -3.51 | 0.39 | 1.43 | -6.13 | -6.45 | -7.47 |
Cell Periphery | -1.54 | -0.96 | -4.47 | -0.08 | -3.92 | 1.86 | 1.34 | -2.51 | -2.66 | -2.59 | -0.69 | 0.56 | -3.03 | -2.74 | -3.09 |
Cytoplasm | 0.38 | -5.14 | 9.65 | 8.95 | 15.48 | -4.64 | 19.76 | -3.26 | 1.15 | -21.23 | -3.35 | 8.04 | 6.25 | 9.10 | -2.02 |
Cytoplasmic Foci | 0.08 | 11.84 | 11.16 | 11.23 | -0.30 | 6.77 | 4.93 | 16.80 | 10.59 | 11.55 | 5.41 | 15.36 | 20.07 | 15.10 | 6.63 |
Eisosomes | 1.86 | 2.66 | 1.61 | -0.81 | -3.71 | 1.02 | -10.25 | -2.25 | -2.73 | 8.37 | 2.07 | -3.62 | -0.10 | -2.73 | 4.48 |
Endoplasmic Reticulum | -1.19 | 2.15 | 1.97 | 5.11 | -0.99 | 4.05 | 3.53 | 6.00 | 5.39 | 6.95 | 2.67 | 5.01 | 5.99 | 6.91 | 4.68 |
Endosome | -4.34 | 1.63 | -4.22 | 0.34 | -5.68 | -0.80 | -9.42 | 3.57 | 3.71 | 11.01 | -3.31 | -3.95 | -1.32 | 1.77 | 2.36 |
Golgi | 2.82 | 8.49 | 6.49 | 6.43 | -2.52 | 0.02 | 18.14 | 7.28 | 5.68 | -3.02 | 1.88 | 16.61 | 10.11 | 8.15 | -3.76 |
Lipid Particles | -1.51 | -7.00 | -8.99 | -1.61 | -3.82 | -0.61 | -3.81 | -5.70 | -3.70 | -1.93 | -1.42 | -7.36 | -9.20 | -4.00 | -3.96 |
Mitochondria | -0.37 | 6.09 | 4.07 | 1.17 | -2.10 | 0.75 | 2.14 | 1.86 | 2.79 | -1.60 | 0.26 | 3.10 | 2.56 | 2.02 | -2.66 |
Mitotic Spindle | -2.84 | -1.74 | -3.64 | -1.49 | -3.09 | -3.10 | -0.93 | -2.48 | -0.41 | -1.58 | -4.23 | -2.58 | -4.50 | -1.66 | -3.42 |
None | -0.85 | 0.75 | 3.86 | 2.44 | 5.39 | 0.81 | 3.01 | 2.54 | 2.01 | -0.98 | -0.31 | 1.71 | 4.55 | 3.21 | 4.35 |
Nuclear Periphery | -3.28 | -11.17 | -5.40 | -2.27 | -0.79 | 0.05 | -10.70 | -3.41 | -3.93 | 9.56 | -2.78 | -14.24 | -6.04 | -3.98 | 5.45 |
Nuclear Periphery Foci | -6.83 | -13.75 | -9.20 | -1.58 | 4.71 | -2.30 | -9.36 | -6.15 | -5.26 | 5.75 | -6.67 | -15.49 | -10.10 | -5.88 | 6.93 |
Nucleolus | -0.73 | 0.17 | -6.38 | -0.99 | -6.73 | 0.87 | -1.28 | -6.17 | -6.26 | 1.14 | -0.67 | -1.38 | -10.08 | -3.72 | 0.83 |
Nucleus | 2.04 | -17.51 | -6.01 | -7.32 | 11.00 | -2.61 | -14.46 | -10.47 | -9.29 | 10.54 | 0.41 | -25.24 | -12.34 | -12.07 | 15.96 |
Peroxisomes | 0.28 | 2.87 | -2.16 | -1.97 | -5.98 | -0.91 | -5.22 | -3.53 | -3.40 | -1.82 | -0.22 | -0.36 | -4.60 | -4.35 | -4.35 |
Vacuole | 0.10 | -9.83 | -23.33 | -23.09 | -18.42 | -0.75 | -24.59 | -13.85 | -13.17 | 20.70 | -0.61 | -23.30 | -26.08 | -25.64 | 1.51 |
Vacuole Periphery | 0.61 | 7.44 | 2.53 | 2.02 | -3.67 | 4.10 | 8.64 | 8.10 | 4.89 | 0.15 | 3.60 | 11.86 | 6.55 | 3.59 | -3.64 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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