Standard name | |
---|---|
Human Ortholog | |
Description | ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 0 | 1 | 1 | – | 2 | 5 | 3 | 6 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 2 | 2 |
Bud | 0 | 1 | 0 | 1 | 0 | 2 | 6 | – | 10 | 10 | 5 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 5 | 1 | 5 | 12 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 20 | 12 | 7 | 8 | 17 | 8 | – | 12 | 21 | 14 | 16 | 2 | 2 | 5 | 14 | 15 | 5 | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 130 | 564 | 382 | 281 | 327 | 390 | 450 | – | 380 | 374 | 225 | 241 | 250 | 299 | 345 | 318 | 250 | 251 | 129 | 599 | 368 | 145 | 174 | 218 |
Endoplasmic Reticulum | 2 | 4 | 7 | 12 | 4 | 9 | 5 | – | 10 | 10 | 6 | 33 | 3 | 3 | 0 | 11 | 13 | 9 | 7 | 15 | 10 | 40 | 37 | 45 |
Endosome | 0 | 9 | 1 | 0 | 2 | 7 | 2 | – | 4 | 5 | 1 | 0 | 0 | 2 | 3 | 3 | 4 | 0 | 0 | 3 | 1 | 3 | 1 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 5 | 1 | 1 | 0 | 1 | 0 | 0 | 4 | 3 |
Mitochondria | 2 | 8 | 0 | 6 | 29 | 66 | 137 | – | 260 | 234 | 229 | 101 | 3 | 1 | 3 | 7 | 2 | 4 | 0 | 3 | 17 | 18 | 13 | 22 |
Nucleus | 3 | 2 | 2 | 1 | 1 | 0 | 2 | – | 0 | 3 | 1 | 1 | 0 | 0 | 2 | 5 | 4 | 1 | 0 | 4 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 39 | 9 | 5 | 18 | 15 | 7 | – | 12 | 31 | 18 | 10 | 0 | 1 | 1 | 49 | 36 | 12 | 1 | 21 | 10 | 23 | 23 | 31 |
Unique Cell Count | 135 | 609 | 400 | 301 | 359 | 463 | 550 | 557 | 567 | 406 | 367 | 258 | 306 | 351 | 370 | 290 | 265 | 144 | 670 | 429 | 240 | 274 | 354 | |
Labelled Cell Count | 140 | 648 | 415 | 313 | 389 | 507 | 621 | 692 | 698 | 503 | 415 | 264 | 309 | 360 | 412 | 326 | 283 | 144 | 670 | 429 | 240 | 274 | 354 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 8.9 | 8.0 | 7.5 | 7.1 | 5.8 | 5.7 | – | 5.1 | 5.2 | 4.8 | 5.6 | 7.0 | 7.0 | 7.0 | 10.9 | 11.7 | 10.4 | 6.9 | 7.0 | 7.3 |
Std Deviation (1e-4) | 1.7 | 1.7 | 1.4 | 1.4 | 1.3 | 1.2 | 1.1 | – | 0.9 | 1.0 | 1.0 | 1.0 | 1.4 | 1.5 | 1.5 | 2.0 | 1.7 | 1.8 | 1.5 | 1.8 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.17 | -0.45 | -0.48 | – | -0.63 | -0.61 | -0.74 | -0.52 | -0.18 | -0.19 | -0.19 | 0.46 | 0.55 | 0.38 | -0.22 | -0.19 | -0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.5 | -0.7 | 0.5 | -1.6 | – | -0.8 | 0.6 | 0 | 1.0 | -1.9 | -2.2 | -1.4 | 0.6 | 1.5 | -0.9 |
Cytoplasm | -1.2 | -2.4 | -5.4 | -6.3 | – | -10.3 | -11.0 | -13.2 | -10.6 | 0.9 | 1.6 | 2.2 | -4.6 | -4.3 | -0.5 |
Endoplasmic Reticulum | 1.8 | -0.7 | 0.2 | -1.1 | – | 0.1 | 0 | 0 | 4.5 | 0 | 0 | 0 | 1.1 | 2.1 | 1.4 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 5.8 | 7.9 | 10.8 | – | 16.0 | 14.8 | 0 | 11.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.6 | 2.1 | 0.9 | -1.2 | – | -0.1 | 2.5 | 1.7 | 0.4 | 0 | 0 | 0 | 5.8 | 5.3 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8405 | 4.382 | 3.182 | 3.2539 | 3.0138 | 3.7367 | 4.3358 | 5.3682 | 4.6436 | 3.8821 | 4.5955 | 4.5952 | 4.0438 | 4.9041 | 4.5062 | 4.1441 | 4.3184 | 4.587 |
Actin | 0.0015 | 0.0028 | 0.0079 | 0.0011 | 0.0051 | 0.0023 | 0.0319 | 0.0029 | 0.0178 | 0.0024 | 0.0284 | 0.0058 | 0.0158 | 0.0006 | 0.0133 | 0.0004 | 0.0067 | 0.0071 |
Bud | 0.0016 | 0.0019 | 0.0023 | 0.001 | 0.0006 | 0.0015 | 0.0017 | 0.0045 | 0.0035 | 0.0012 | 0.0092 | 0.0008 | 0.0067 | 0.002 | 0.0057 | 0.0006 | 0.0041 | 0.0036 |
Bud Neck | 0.0008 | 0.0005 | 0.0008 | 0.0013 | 0.0012 | 0.0033 | 0.0045 | 0.0004 | 0.0012 | 0.0017 | 0.0031 | 0.0022 | 0.0047 | 0.0003 | 0.0011 | 0.0011 | 0.0022 | 0.0013 |
Bud Periphery | 0.0008 | 0.0019 | 0.0035 | 0.0005 | 0.0006 | 0.002 | 0.0016 | 0.0052 | 0.0041 | 0.0008 | 0.0087 | 0.0007 | 0.002 | 0.0055 | 0.002 | 0.0003 | 0.0046 | 0.0016 |
Bud Site | 0.0027 | 0.0109 | 0.0025 | 0.0004 | 0.0007 | 0.0017 | 0.0084 | 0.0072 | 0.0101 | 0.0007 | 0.0479 | 0.0012 | 0.0065 | 0.0024 | 0.0059 | 0.0012 | 0.0275 | 0.0002 |
Cell Periphery | 0.0005 | 0.0006 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0004 | 0.0005 | 0.0002 | 0.001 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0002 |
Cytoplasm | 0.7054 | 0.823 | 0.7218 | 0.6696 | 0.7014 | 0.7399 | 0.5261 | 0.861 | 0.7454 | 0.7655 | 0.5263 | 0.7979 | 0.6102 | 0.9005 | 0.7878 | 0.7477 | 0.6646 | 0.8205 |
Cytoplasmic Foci | 0.0384 | 0.0292 | 0.0403 | 0.0551 | 0.0373 | 0.0505 | 0.0549 | 0.0227 | 0.0302 | 0.0304 | 0.0641 | 0.0305 | 0.0612 | 0.0192 | 0.0268 | 0.0479 | 0.0449 | 0.0332 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0014 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0273 | 0.0103 | 0.0118 | 0.0184 | 0.0221 | 0.0201 | 0.0302 | 0.0139 | 0.015 | 0.0147 | 0.0068 | 0.0211 | 0.0288 | 0.0115 | 0.0169 | 0.009 | 0.0129 | 0.0142 |
Endosome | 0.0934 | 0.0618 | 0.1144 | 0.1313 | 0.1061 | 0.1179 | 0.1499 | 0.0404 | 0.0778 | 0.0854 | 0.1231 | 0.0843 | 0.1208 | 0.0318 | 0.0748 | 0.1341 | 0.0756 | 0.0672 |
Golgi | 0.0102 | 0.0129 | 0.0064 | 0.0147 | 0.0094 | 0.0114 | 0.0319 | 0.0058 | 0.0196 | 0.0106 | 0.0531 | 0.0113 | 0.0152 | 0.0031 | 0.0054 | 0.0045 | 0.0107 | 0.0067 |
Lipid Particles | 0.0023 | 0.0002 | 0.0007 | 0.0005 | 0.0118 | 0.0004 | 0.0152 | 0.0004 | 0.0081 | 0.0009 | 0.0118 | 0.002 | 0.0094 | 0.0001 | 0.0027 | 0.0007 | 0.0042 | 0.0014 |
Mitochondria | 0.0099 | 0.0144 | 0.0073 | 0.0051 | 0.006 | 0.009 | 0.0242 | 0.0019 | 0.0197 | 0.0023 | 0.0656 | 0.0078 | 0.0069 | 0.0021 | 0.005 | 0.0021 | 0.0314 | 0.0053 |
None | 0.06 | 0.0132 | 0.0501 | 0.0718 | 0.0475 | 0.0228 | 0.0294 | 0.0166 | 0.0129 | 0.0467 | 0.0046 | 0.0127 | 0.0548 | 0.01 | 0.0212 | 0.0235 | 0.0877 | 0.0223 |
Nuclear Periphery | 0.0054 | 0.0011 | 0.0018 | 0.0028 | 0.0201 | 0.0019 | 0.021 | 0.0015 | 0.0027 | 0.0063 | 0.0059 | 0.0035 | 0.0087 | 0.001 | 0.0037 | 0.0022 | 0.002 | 0.0029 |
Nucleolus | 0.0018 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0038 | 0.0001 | 0.0002 | 0.0001 | 0.0017 | 0.0003 | 0.0007 | 0 | 0.0015 | 0.0001 | 0.0003 | 0.0001 |
Nucleus | 0.0083 | 0.0015 | 0.0023 | 0.0019 | 0.0043 | 0.0018 | 0.0257 | 0.0019 | 0.0018 | 0.0031 | 0.0058 | 0.0044 | 0.016 | 0.0013 | 0.0056 | 0.0021 | 0.002 | 0.0016 |
Peroxisomes | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0011 | 0.0007 | 0.0059 | 0.0016 | 0.0101 | 0.0004 | 0.01 | 0.002 | 0.0084 | 0.0001 | 0.0014 | 0.0004 | 0.0081 | 0.0035 |
Punctate Nuclear | 0.0051 | 0.0002 | 0.0008 | 0.0004 | 0.005 | 0.0011 | 0.0129 | 0.0005 | 0.0009 | 0.0006 | 0.0036 | 0.0016 | 0.0032 | 0.0002 | 0.0036 | 0.0009 | 0.0019 | 0.0007 |
Vacuole | 0.0207 | 0.0106 | 0.0197 | 0.0178 | 0.0133 | 0.0084 | 0.015 | 0.0097 | 0.0153 | 0.0219 | 0.0133 | 0.007 | 0.0165 | 0.0064 | 0.0121 | 0.0171 | 0.0064 | 0.0047 |
Vacuole Periphery | 0.0031 | 0.0026 | 0.0043 | 0.0055 | 0.005 | 0.003 | 0.0049 | 0.0015 | 0.003 | 0.0042 | 0.0043 | 0.0027 | 0.0031 | 0.0014 | 0.0026 | 0.0038 | 0.0019 | 0.0018 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.1981 | 24.8383 | 30.6144 | 23.2702 | 24.1183 | 12.2376 | 23.6242 | 24.5635 | 21.145 | 25.1125 |
Translational Efficiency | 1.1944 | 1.1935 | 0.9273 | 1.0556 | 1.0724 | 1.8076 | 1.1826 | 0.8048 | 0.8409 | 0.9072 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2268 | 1151 | 177 | 1431 | 1896 | 803 | 1677 | 95 | 4164 | 1954 | 1854 | 1526 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 711.76 | 869.57 | 1075.30 | 995.04 | 804.10 | 861.78 | 954.65 | 1193.44 | 753.81 | 866.37 | 966.17 | 1007.39 |
Standard Deviation | 114.80 | 164.00 | 119.35 | 154.69 | 119.54 | 149.87 | 161.84 | 1327.76 | 125.69 | 158.40 | 162.20 | 366.73 |
Intensity Change Log 2 | — | 0.288911 | 0.595276 | 0.483364 | — | 0.099945 | 0.247597 | 0.569679 | — | 0.191761 | 0.421308 | 0.529793 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000185 | 0.000394 | 0.000420 | 0.000607 | 0.000223 | 0.001158 | 0.000142 | 0.000358 | 0.000203 | 0.000708 | 0.000169 | 0.000591 |
Bud Neck | 0.011302 | 0.004599 | 0.002767 | 0.003310 | 0.006057 | 0.012316 | 0.003515 | 0.002209 | 0.008914 | 0.007770 | 0.003443 | 0.003241 |
Bud Site | 0.021141 | 0.027776 | 0.010212 | 0.031735 | 0.022650 | 0.029099 | 0.008508 | 0.028195 | 0.021829 | 0.028320 | 0.008671 | 0.031514 |
Cell Periphery | 0.000285 | 0.000155 | 0.000163 | 0.000136 | 0.000391 | 0.000315 | 0.000481 | 0.000155 | 0.000334 | 0.000221 | 0.000450 | 0.000137 |
Cytoplasm | 0.578195* | 0.558695* | 0.362498* | 0.685625* | 0.513277* | 0.410450* | 0.575218* | 0.375030* | 0.548636* | 0.497773* | 0.554910* | 0.666289* |
Cytoplasmic Foci | 0.224191* | 0.242822* | 0.126173 | 0.093997 | 0.268972* | 0.302802* | 0.093697 | 0.142499 | 0.244581* | 0.267471* | 0.096797 | 0.097016 |
Eisosomes | 0.000116 | 0.000043 | 0.000081 | 0.000016 | 0.000091 | 0.000221 | 0.000154 | 0.000050 | 0.000104 | 0.000116 | 0.000147 | 0.000018 |
Endoplasmic Reticulum | 0.008362 | 0.004118 | 0.003750 | 0.001075 | 0.011939 | 0.004105 | 0.002445 | 0.005387 | 0.009991 | 0.004112 | 0.002570 | 0.001343 |
Endosome | 0.014535 | 0.026896 | 0.037015 | 0.010935 | 0.025951 | 0.051954 | 0.014426 | 0.034602 | 0.019733 | 0.037194 | 0.016583 | 0.012408 |
Golgi | 0.016295 | 0.011957 | 0.000699 | 0.003020 | 0.021837 | 0.025120 | 0.001659 | 0.001066 | 0.018818 | 0.017366 | 0.001567 | 0.002898 |
Lipid Particles | 0.006351 | 0.004369 | 0.007491 | 0.003016 | 0.005170 | 0.009916 | 0.004336 | 0.004818 | 0.005814 | 0.006649 | 0.004637 | 0.003128 |
Mitochondria | 0.012662 | 0.004766 | 0.000113 | 0.001832 | 0.007859 | 0.010169 | 0.000333 | 0.000080 | 0.010475 | 0.006987 | 0.000312 | 0.001723 |
Mitotic Spindle | 0.000857 | 0.002441 | 0.003925 | 0.011968 | 0.000667 | 0.004240 | 0.003116 | 0.000576 | 0.000771 | 0.003180 | 0.003193 | 0.011258 |
None | 0.009991 | 0.002040 | 0.000553 | 0.000577 | 0.002005 | 0.000687 | 0.005766 | 0.000635 | 0.006355 | 0.001484 | 0.005268 | 0.000581 |
Nuclear Periphery | 0.000187 | 0.000245 | 0.002312 | 0.000181 | 0.000137 | 0.000253 | 0.000689 | 0.000584 | 0.000165 | 0.000249 | 0.000844 | 0.000206 |
Nuclear Periphery Foci | 0.000322 | 0.000570 | 0.008797 | 0.001358 | 0.000458 | 0.000621 | 0.002215 | 0.004588 | 0.000384 | 0.000591 | 0.002843 | 0.001559 |
Nucleolus | 0.000663 | 0.000365 | 0.000539 | 0.000233 | 0.000244 | 0.000589 | 0.000533 | 0.000256 | 0.000472 | 0.000457 | 0.000533 | 0.000234 |
Nucleus | 0.032186 | 0.018390 | 0.046548 | 0.022200 | 0.014615 | 0.021392 | 0.114051 | 0.050052 | 0.024186 | 0.019624 | 0.107607 | 0.023934 |
Peroxisomes | 0.001949 | 0.002384 | 0.001074 | 0.002105 | 0.001542 | 0.004859 | 0.000792 | 0.001588 | 0.001764 | 0.003401 | 0.000819 | 0.002073 |
Vacuole | 0.048845 | 0.074852 | 0.383245* | 0.123443 | 0.078123 | 0.099944 | 0.166524 | 0.345198* | 0.062176 | 0.085163 | 0.187214 | 0.137248 |
Vacuole Periphery | 0.011379 | 0.012122 | 0.001627 | 0.002634 | 0.017788 | 0.009790 | 0.001400 | 0.002075 | 0.014297 | 0.011164 | 0.001421 | 0.002599 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.13 | -3.83 | -4.56 | -1.49 | -0.91 | -2.11 | 3.19 | -0.76 | 1.76 | -1.19 | -2.70 | 0.98 | -4.45 | 0.91 | -4.63 |
Bud Neck | 7.76 | 10.41 | 10.02 | 2.37 | -1.28 | -5.10 | 4.88 | 6.41 | 8.10 | 1.92 | 1.65 | 9.91 | 11.07 | 7.45 | -0.03 |
Bud Site | -3.29 | 7.18 | -1.83 | 0.95 | -6.54 | -2.89 | 10.77 | -0.31 | 0.43 | -2.03 | -4.34 | 12.58 | -1.65 | 1.31 | -8.09 |
Cell Periphery | 6.79 | 5.91 | 9.45 | 2.86 | 3.02 | 0.77 | -0.19 | 0.98 | 0.57 | 1.34 | 2.45 | -0.83 | 4.72 | 5.40 | 5.33 |
Cytoplasm | 2.02 | 10.62 | -5.40 | -6.17 | -12.54 | 8.88 | -6.03 | 4.26 | 0.85 | 6.07 | 6.61 | 0.04 | -7.56 | -11.57 | -6.39 |
Cytoplasmic Foci | -2.51 | 11.92 | 28.03 | 23.39 | 3.69 | -3.66 | 32.29 | 9.32 | 10.70 | -3.27 | -3.90 | 36.87 | 36.48 | 31.67 | 2.09 |
Eisosomes | 8.73 | 2.93 | 13.85 | 6.30 | 6.55 | -3.34 | -2.26 | 4.05 | 4.29 | 4.13 | -0.73 | -1.40 | 18.29 | 6.03 | 6.69 |
Endoplasmic Reticulum | 3.44 | 3.80 | 7.39 | 4.04 | 4.62 | 5.92 | 8.26 | 3.72 | -0.24 | -1.37 | 6.47 | 9.53 | 11.50 | 5.23 | 3.54 |
Endosome | -8.01 | -5.64 | 6.78 | 11.83 | 7.51 | -9.61 | 10.20 | -0.58 | 3.31 | -2.59 | -11.86 | 4.88 | 10.83 | 17.83 | 5.06 |
Golgi | 4.32 | 23.42 | 20.02 | 12.13 | -4.96 | -1.36 | 20.98 | 22.89 | 10.92 | 0.58 | 1.36 | 25.64 | 28.78 | 14.77 | -1.14 |
Lipid Particles | 2.59 | -0.49 | 7.54 | 2.25 | 3.89 | -3.33 | 2.89 | 0.41 | 3.05 | -0.79 | -1.23 | 3.93 | 8.46 | 5.02 | 3.11 |
Mitochondria | 5.72 | 14.54 | 10.91 | 2.84 | -1.81 | -1.31 | 9.78 | 10.08 | 6.51 | 5.77 | 3.27 | 17.33 | 11.48 | 5.46 | -1.54 |
Mitotic Spindle | -1.88 | -1.78 | -6.78 | -5.35 | -1.23 | -2.72 | -4.54 | 0.16 | 2.74 | 4.55 | -3.30 | -4.80 | -6.85 | -4.83 | -3.77 |
None | 6.74 | 8.89 | 8.83 | 2.39 | -0.69 | 2.37 | -2.68 | 2.26 | 0.30 | 4.25 | 6.93 | 1.68 | 9.02 | 2.30 | 4.35 |
Nuclear Periphery | -0.84 | -2.11 | -5.18 | -2.48 | 1.85 | -1.85 | -6.73 | -3.35 | -2.32 | 0.74 | -1.75 | -5.85 | -6.48 | -3.27 | 3.33 |
Nuclear Periphery Foci | -3.80 | -8.84 | -10.84 | -8.68 | 7.17 | -0.70 | -6.69 | -3.37 | -3.22 | -1.89 | -1.73 | -11.94 | -9.34 | -8.41 | 3.41 |
Nucleolus | 4.22 | 0.86 | 6.83 | 1.87 | 2.23 | -2.12 | -4.01 | 0.06 | 2.06 | 3.26 | 0.05 | -0.48 | 5.55 | 2.46 | 3.73 |
Nucleus | 5.37 | -2.05 | -1.25 | -5.61 | 1.73 | -2.22 | -18.60 | -3.38 | -2.62 | 4.79 | 2.16 | -16.92 | -5.41 | -6.05 | 13.25 |
Peroxisomes | -1.79 | 6.72 | 1.56 | 2.57 | -2.78 | -7.58 | 7.53 | 0.29 | 5.96 | -1.73 | -7.02 | 10.63 | 0.80 | 6.20 | -4.29 |
Vacuole | -6.70 | -16.64 | -23.23 | -16.86 | 9.59 | -3.77 | -18.63 | -9.05 | -8.22 | -5.44 | -6.93 | -26.22 | -22.86 | -17.48 | 0.54 |
Vacuole Periphery | -0.49 | 13.80 | 12.40 | 8.33 | -1.02 | 7.41 | 18.93 | 16.58 | 9.73 | -1.06 | 3.43 | 22.64 | 19.85 | 11.41 | -1.30 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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