Standard name
Human Ortholog
Description Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 0 0 – 0 0.05 0 0.05 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.91 0.96 0.93 0.94 0.85 0.82 0.72 0.7 0.61 – 0.57 0.54 0.93 0.96 0.97 0.84 0.86 0.84 0.91 0.93 0.87 0.87 0.87 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0.06 0.05 0 0 0 0.05 0.05 0 0 0 0 0.07 0.06 0.14
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.12 0.15 0.26 0.33 0.33 0.48 – 0.56 0.56 0 0 0.1 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0.13 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 2 0 3 7 3 4 2 – 4 5 3 2 1 3 1 3 0 0 2 0 0 1
Bud 0 2 6 1 2 5 5 5 6 – 7 8 1 0 3 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 26 6 10 3 14 8 16 6 14 – 16 28 1 12 6 5 7 11 0 0 0 0 0 0
Cytoplasm 458 370 331 279 286 369 346 375 251 – 243 296 188 245 273 274 168 200 519 369 331 151 180 172
Endoplasmic Reticulum 18 14 12 9 13 14 19 20 15 – 24 27 5 3 2 16 9 9 12 7 15 12 12 31
Endosome 1 0 0 0 0 0 2 1 0 – 0 0 3 1 0 5 1 1 2 0 1 0 1 2
Golgi 3 1 1 0 0 0 0 0 0 – 0 0 0 0 1 3 3 2 0 0 2 0 1 1
Mitochondria 5 0 9 35 49 117 161 176 199 – 239 307 3 4 27 6 1 3 8 1 9 2 1 1
Nucleus 2 1 1 0 0 0 2 2 1 – 0 3 0 2 0 5 3 3 2 4 1 0 0 0
Nuclear Periphery 0 2 0 0 0 0 1 0 0 – 0 1 0 0 1 1 0 0 3 0 0 0 0 1
Nucleolus 1 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Vac/Vac Membrane 17 4 6 0 8 5 8 13 14 – 9 16 6 2 0 32 25 34 17 10 10 3 5 5
Unique Cell Count 504 385 355 297 336 451 481 532 412 430 546 202 254 281 326 195 237 569 399 382 174 208 224
Labelled Cell Count 532 402 378 327 375 525 563 602 502 542 691 210 271 315 350 220 267 569 399 382 174 208 224


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 11.6 8.4 7.7 8.0 7.2 6.9 6.4 6.1 – 5.8 5.6 9.9 9.7 8.9 11.5 12.9 12.1 10.9 11.9 12.3
Std Deviation (1e-4) 4.5 1.9 1.3 1.1 1.4 1.1 1.3 1.1 1.3 – 1.2 1.1 1.5 1.5 1.4 2.8 3.0 3.2 1.8 2.1 2.2
Intensity Change (Log2) – – – -0.13 -0.07 -0.21 -0.28 -0.39 -0.46 – -0.52 -0.59 0.24 0.2 0.08 0.46 0.63 0.53 0.38 0.51 0.55

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 -0.3 – -0.1 -0.3 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -1.6 1.0 -1.0 0.4 -1.9 0.5 – 0.7 1.7 0 1.2 -0.5 -1.1 0.5 1.2
Cytoplasm 0.4 -3.5 -4.8 -7.8 -8.2 -10.4 – -11.6 -12.4 -0.1 1.7 2.2 -3.8 -2.7 -3.5
Endoplasmic Reticulum -0.3 0.3 -0.2 0.4 0.3 0.2 – 1.5 1.1 -0.6 -1.7 -2.3 1.0 0.7 0.3
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 4.7 5.7 9.1 11.0 11.0 14.2 – 15.9 16.5 0 -0.8 3.8 -0.6 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0.6 0 0 0.8 1.5 – 0.4 1.2 0 0 0 4.6 5.4 6.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.7253 13.9374 12.5071 11.1939 11.6014 12.3356 9.5185 12.4463 11.1359 10.5066 10.0554 11.2516 11.1996 12.6508 11.9623 11.577 11.0361 12.0383
Actin 0.001 0.001 0.0009 0.0023 0.0011 0.001 0.0021 0.0002 0.0016 0.0003 0.0001 0.0004 0.0013 0.0024 0.001 0.0009 0.0002 0.0009
Bud 0.0011 0.0007 0.0013 0.0022 0.0006 0.0008 0.0004 0.0004 0.0006 0.0006 0.0003 0.0002 0.0005 0.0007 0.0006 0.0005 0.0004 0.0005
Bud Neck 0.0011 0.0008 0.0012 0.0014 0.0016 0.0033 0.0004 0.0002 0.0004 0.0006 0.0004 0.0011 0.0004 0.0004 0.0005 0.0006 0.0006 0.0012
Bud Periphery 0.0022 0.0008 0.0015 0.0048 0.0005 0.0017 0.0003 0.0002 0.0004 0.0003 0.0002 0.0001 0.0004 0.0002 0.0004 0.0003 0.0001 0.0005
Bud Site 0.0009 0.0014 0.0016 0.0011 0.0003 0.0003 0.0005 0.0004 0.0006 0.0004 0.0001 0.0001 0.0007 0.0018 0.0006 0.0003 0.0002 0.0002
Cell Periphery 0.0035 0.002 0.0018 0.0042 0.0005 0.0024 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0009 0.0003 0.0003 0.0002 0 0.0003
Cytoplasm 0.7853 0.9037 0.8294 0.7871 0.8287 0.8349 0.8521 0.9576 0.9025 0.8631 0.8714 0.8865 0.8434 0.9118 0.8834 0.8079 0.9048 0.8635
Cytoplasmic Foci 0.008 0.0064 0.0072 0.013 0.0117 0.0102 0.0204 0.0086 0.0173 0.0218 0.0163 0.0184 0.0172 0.0178 0.0152 0.0168 0.0199 0.0215
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0982 0.0435 0.0724 0.0321 0.0832 0.0846 0.0434 0.0069 0.0099 0.0076 0.0188 0.0272 0.0521 0.0161 0.0193 0.0347 0.0034 0.0267
Endosome 0.0324 0.0165 0.0313 0.0669 0.0398 0.0274 0.0478 0.0153 0.044 0.08 0.0697 0.0473 0.0543 0.0301 0.0523 0.0822 0.0576 0.0549
Golgi 0.0065 0.0032 0.0036 0.0085 0.0061 0.0048 0.008 0.0022 0.0043 0.0036 0.0034 0.0073 0.0093 0.0079 0.005 0.0106 0.0033 0.0127
Lipid Particles 0.0008 0.0002 0.0004 0.0002 0.0003 0.0002 0.0034 0 0.0004 0 0 0 0.0003 0.0002 0.0002 0.0002 0 0.0001
Mitochondria 0.0136 0.0029 0.0047 0.0044 0.0036 0.012 0.0058 0.0028 0.0024 0.0021 0.0023 0.0051 0.0051 0.0025 0.0033 0.0037 0.0014 0.0099
None 0.0087 0.0007 0.0007 0.0007 0.0007 0.0008 0.003 0.0008 0.0014 0.0012 0.0015 0.001 0.0017 0.0009 0.0007 0.0006 0.0005 0.0008
Nuclear Periphery 0.0041 0.0015 0.0045 0.0043 0.0034 0.002 0.0021 0.0004 0.0009 0.0012 0.002 0.0006 0.0016 0.0008 0.0014 0.0017 0.0005 0.0007
Nucleolus 0.0001 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.002 0.0013 0.0021 0.0024 0.0015 0.0013 0.001 0.0005 0.001 0.001 0.0012 0.0007 0.0008 0.0008 0.0009 0.0008 0.0007 0.0007
Peroxisomes 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.001 0 0.0019 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Punctate Nuclear 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0005 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001
Vacuole 0.0255 0.0115 0.0295 0.0516 0.013 0.0089 0.0058 0.0025 0.0081 0.0115 0.0082 0.0025 0.0078 0.0042 0.0114 0.0335 0.0045 0.0032
Vacuole Periphery 0.0047 0.0019 0.0057 0.0127 0.0031 0.0032 0.0016 0.0006 0.0019 0.0044 0.0038 0.0012 0.0021 0.001 0.0033 0.004 0.0018 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.1271 27.3142 33.9069 37.482 25.3615 25.1154 31.8762 34.4054 39.1013 34.6752
Translational Efficiency 1.5402 1.3103 1.1063 1.2249 1.2374 1.4417 1.087 0.8938 0.8978 0.9892

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2186 671 279 988 1750 2089 129 870 3936 2760 408 1858

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 920.35 1205.68 1481.50 1428.77 962.66 1099.81 1443.23 1437.97 939.16 1125.55 1469.40 1433.08
Standard Deviation 123.03 185.52 180.70 212.49 183.00 163.12 158.24 233.29 154.07 174.84 174.82 222.52
Intensity Change Log 2 — 0.389593 0.686804 0.634519 — 0.192156 0.584203 0.578935 — 0.292033 0.635263 0.606370

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000230 0.000432 0.000451 0.000917 0.000191 0.000284 0.001170 0.000551 0.000212 0.000320 0.000678 0.000746
Bud Neck 0.001207 0.000506 0.002473 0.001603 0.000875 0.000730 0.002140 0.001309 0.001059 0.000675 0.002368 0.001465
Bud Site 0.034697 0.044553 0.020147 0.036882 0.025084 0.029220 0.038059 0.036567 0.030423 0.032948 0.025811 0.036734
Cell Periphery 0.000384 0.000423 0.000215 0.001730 0.000237 0.000180 0.000440 0.001865 0.000319 0.000239 0.000286 0.001793
Cytoplasm 0.616151 0.608408 0.469681 0.828863 0.592328 0.637450 0.630707 0.834191 0.605559 0.630389 0.520594 0.831358
Cytoplasmic Foci 0.223705 0.252706 0.105883 0.039297 0.241362 0.211932 0.145525 0.043161 0.231555 0.221845 0.118417 0.041107
Eisosomes 0.000026 0.000009 0.000010 0.000007 0.000014 0.000011 0.000014 0.000005 0.000020 0.000010 0.000011 0.000006
Endoplasmic Reticulum 0.009954 0.005013 0.005377 0.000737 0.009462 0.003782 0.003588 0.000637 0.009735 0.004081 0.004811 0.000690
Endosome 0.009966 0.012490 0.035667 0.005629 0.015984 0.014798 0.022171 0.003974 0.012642 0.014237 0.031400 0.004854
Golgi 0.023062 0.030369 0.006900 0.010755 0.029161 0.027219 0.007103 0.009694 0.025773 0.027985 0.006964 0.010258
Lipid Particles 0.000872 0.000628 0.004649 0.005533 0.001253 0.000772 0.004065 0.007821 0.001041 0.000737 0.004464 0.006605
Mitochondria 0.007081 0.000677 0.000054 0.000654 0.003054 0.002790 0.000021 0.002145 0.005290 0.002276 0.000044 0.001352
Mitotic Spindle 0.000335 0.005957 0.006883 0.003272 0.000800 0.002401 0.004327 0.002356 0.000542 0.003266 0.006075 0.002843
None 0.000832 0.000124 0.000068 0.000096 0.001358 0.000274 0.000047 0.000141 0.001066 0.000238 0.000061 0.000117
Nuclear Periphery 0.000024 0.000018 0.000277 0.000029 0.000156 0.000035 0.000089 0.000027 0.000082 0.000031 0.000217 0.000028
Nuclear Periphery Foci 0.000116 0.000334 0.005311 0.001143 0.000455 0.000198 0.003584 0.001253 0.000267 0.000231 0.004765 0.001194
Nucleolus 0.000054 0.000061 0.000101 0.000077 0.000298 0.000384 0.000039 0.000105 0.000162 0.000305 0.000081 0.000090
Nucleus 0.002056 0.001214 0.004672 0.002144 0.004111 0.001608 0.003854 0.002139 0.002970 0.001512 0.004413 0.002141
Peroxisomes 0.000571 0.000570 0.000607 0.001130 0.000471 0.000651 0.001069 0.000862 0.000526 0.000631 0.000753 0.001004
Vacuole 0.020812 0.014490 0.314385 0.054226 0.032469 0.036056 0.128708 0.046388 0.025995 0.030812 0.255678 0.050556
Vacuole Periphery 0.047867 0.021019 0.016188 0.005277 0.040880 0.029229 0.003279 0.004810 0.044760 0.027233 0.012107 0.005058

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.63 -3.36 -3.22 -2.12 -2.00 -2.45 -3.77 -7.32 -4.73 2.48 -2.97 -4.93 -4.63 -3.60 -0.10
Bud Neck 7.31 -6.21 -3.67 -9.91 3.63 1.53 -4.22 -4.01 -5.69 2.60 5.43 -7.81 -5.26 -10.20 4.81
Bud Site -3.84 6.12 -0.66 2.44 -5.09 -2.48 -2.46 -4.01 -2.55 0.43 -1.88 1.70 -3.13 -1.72 -3.51
Cell Periphery -0.85 5.85 -2.94 -2.84 -3.34 3.65 -1.91 -7.43 -7.71 -5.82 4.61 0.90 -5.65 -5.98 -5.74
Cytoplasm 0.61 7.48 -23.58 -16.42 -17.90 -4.73 -1.15 -24.83 -20.72 -8.21 -3.41 5.50 -34.16 -26.76 -19.30
Cytoplasmic Foci -3.11 15.30 40.61 24.81 9.48 4.40 7.39 35.90 32.76 8.80 1.93 16.87 54.03 42.00 12.66
Eisosomes 10.39 8.86 11.31 1.59 2.33 3.08 -0.68 9.97 7.61 5.45 10.35 6.94 14.37 5.94 5.16
Endoplasmic Reticulum 3.63 3.79 9.31 4.47 5.38 4.48 4.18 7.50 6.59 3.19 6.53 5.29 11.93 7.82 6.28
Endosome -2.65 -8.86 6.09 6.50 10.29 1.06 -1.65 12.75 14.70 5.52 -2.34 -8.12 13.56 14.57 11.74
Golgi -4.33 16.40 7.50 9.08 -1.95 1.38 15.49 15.40 12.48 -1.80 -2.14 23.05 14.61 13.88 -2.69
Lipid Particles 4.01 -6.75 -10.10 -10.58 -1.36 3.59 -5.39 -6.07 -6.53 -3.14 3.84 -8.18 -9.99 -10.51 -3.11
Mitochondria 15.70 17.75 12.48 0.21 -1.65 0.64 14.84 0.71 0.52 -1.42 8.34 21.86 5.52 1.36 -1.79
Mitotic Spindle -2.68 -3.51 -2.28 1.19 1.66 -2.76 -3.11 -1.68 -0.64 0.32 -4.08 -4.16 -2.77 -0.01 1.60
None 3.53 3.85 3.71 1.08 -1.22 3.23 3.94 3.66 1.86 -1.97 4.38 5.45 5.14 2.45 -2.27
Nuclear Periphery 0.91 -5.79 -0.77 -1.28 5.57 1.98 0.99 2.10 0.43 3.02 1.81 -3.39 1.93 0.18 6.04
Nuclear Periphery Foci -3.26 -8.51 -13.52 -8.46 6.65 2.34 -6.58 -6.67 -9.78 4.83 0.22 -10.09 -13.74 -13.22 7.85
Nucleolus -0.29 -3.60 -4.27 -0.27 1.78 -0.66 4.24 3.00 1.47 -2.44 -1.10 2.69 2.45 1.50 -0.36
Nucleus 2.24 -4.48 -1.87 -3.49 3.79 3.52 -0.33 2.50 -2.68 1.71 3.95 -2.83 1.57 -4.15 4.00
Peroxisomes -0.11 -0.60 -5.11 -4.13 -4.24 -3.02 -2.31 -4.43 -2.13 0.88 -2.31 -2.45 -6.71 -4.49 -1.91
Vacuole 3.51 -17.16 -12.06 -13.18 14.65 -1.45 -6.01 -4.89 -3.98 4.90 -2.92 -17.12 -12.16 -9.49 14.79
Vacuole Periphery 9.78 11.45 19.36 7.89 4.82 4.82 18.10 17.69 15.56 -1.63 9.48 16.66 26.13 17.18 4.41
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Ski2

Ski2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ski2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available