Standard name | |
---|---|
Human Ortholog | |
Description | Dock family protein (Dedicator Of CytoKinesis), homolog of human DOCK1; upstream component for regulation through the small GTPase Rho5p; may form a complex with Lmo1p that acts as a GEF for Rho5p; interacts with Ino4p; cytoplasmic protein that relocates to mitochondria under oxidative stress; implicated in mitophagy; not an essential protein; DOCK proteins act as guanine nucleotide exchange factors |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 4 | 4 | 6 | 4 | 14 | 9 | 4 | 2 | 4 | 2 | 4 | 7 | 6 | 5 | – | – | – | 31 | 15 | 56 | 28 | 54 | 85 |
Bud | 2 | 2 | 22 | 20 | 19 | 46 | 49 | 50 | 31 | 56 | 30 | 41 | 7 | 4 | 21 | – | – | – | 7 | 1 | 7 | 4 | 11 | 7 |
Bud Neck | 0 | 0 | 11 | 7 | 13 | 18 | 8 | 20 | 9 | 11 | 8 | 9 | 0 | 0 | 1 | – | – | – | 12 | 14 | 16 | 10 | 17 | 27 |
Bud Site | 0 | 0 | 0 | 1 | 9 | 10 | 10 | 17 | 13 | 11 | 6 | 4 | 0 | 0 | 1 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 31 | 12 | 16 | 22 | 19 | 24 | 17 | 19 | 41 | 26 | 31 | 24 | 63 | 95 | 111 | – | – | – | 15 | 11 | 41 | 16 | 45 | 94 |
Cytoplasm | 274 | 185 | 509 | 364 | 501 | 596 | 626 | 671 | 475 | 538 | 318 | 325 | 386 | 456 | 535 | – | – | – | 190 | 127 | 269 | 223 | 262 | 254 |
Endoplasmic Reticulum | 1 | 5 | 2 | 1 | 0 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 3 | 4 | – | – | – | 38 | 6 | 16 | 5 | 25 | 32 |
Endosome | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | – | – | – | 7 | 4 | 7 | 2 | 2 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 4 | 0 | 2 | 1 | 0 | 1 |
Mitochondria | 11 | 4 | 5 | 25 | 34 | 108 | 162 | 153 | 159 | 202 | 119 | 141 | 0 | 1 | 4 | – | – | – | 17 | 9 | 72 | 17 | 24 | 29 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 4 | 2 | 5 | 1 | 4 | 0 | 1 | 1 | – | – | – | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | – | – | – | 1 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 5 | 0 | 6 | 1 | 14 | 11 | 19 | 26 | 19 | 15 | 5 | 4 | 2 | 0 | 2 | – | – | – | 1 | 0 | 3 | 0 | 0 | 2 |
Vac/Vac Membrane | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 3 | 0 | 1 | 2 | – | – | – | 3 | 8 | 6 | 6 | 7 | 6 |
Unique Cell Count | 305 | 197 | 532 | 385 | 548 | 704 | 784 | 836 | 601 | 705 | 428 | 450 | 397 | 471 | 557 | 338 | 206 | 521 | 323 | 462 | 557 | |||
Labelled Cell Count | 342 | 214 | 575 | 447 | 613 | 831 | 903 | 971 | 753 | 871 | 521 | 562 | 467 | 570 | 693 | 338 | 206 | 521 | 323 | 462 | 557 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 5.4 | 3.8 | 3.6 | 3.8 | 3.5 | 3.5 | 3.6 | 3.2 | 3.1 | 3.2 | 3.2 | 5.6 | 5.7 | 5.1 | – | – | – | 5.7 | 5.9 | 6.2 |
Std Deviation (1e-4) | 1.0 | 0.7 | 0.8 | 0.9 | 1.1 | 0.8 | 0.9 | 1.3 | 1.1 | 0.9 | 0.9 | 1.1 | 0.9 | 1.1 | 1.0 | – | – | – | 1.7 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.01 | -0.11 | -0.13 | -0.06 | -0.25 | -0.28 | -0.26 | -0.26 | 0.57 | 0.59 | 0.42 | – | – | – | 0.6 | 0.63 | 0.72 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.1 | -3.3 | -0.3 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.1 | -3.0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.0 | 8.6 | 8.7 | – | – | – |
Cytoplasm | -0.8 | -2.8 | -6.2 | -8.2 | -8.1 | -8.3 | -9.4 | -9.5 | -10.2 | 1.2 | 0.9 | 0.3 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6452 | 3.0299 | 2.6697 | 2.5678 | 2.7927 | 2.727 | 0.4399 | 0.9277 | 0.5499 | 0.2875 | 0.8252 | 0.5987 | 0.2613 | 1.3192 | 0.9019 | 0.9028 | 1.2807 | 0.866 |
Actin | 0.0468 | 0.028 | 0.0185 | 0.0873 | 0.0306 | 0.0128 | 0.0337 | 0.0057 | 0.0328 | 0.0336 | 0.0366 | 0.0299 | 0.0212 | 0.0035 | 0.0526 | 0.0296 | 0.0144 | 0.0238 |
Bud | 0.0017 | 0.0011 | 0.0047 | 0.0016 | 0.0017 | 0.0021 | 0.0044 | 0.0034 | 0.0045 | 0.016 | 0.0076 | 0.0015 | 0.0014 | 0.0024 | 0.0011 | 0.0012 | 0.0006 | 0.0013 |
Bud Neck | 0.0137 | 0.0029 | 0.0055 | 0.0031 | 0.0221 | 0.0246 | 0.012 | 0.0032 | 0.0137 | 0.0077 | 0.0086 | 0.0281 | 0.0017 | 0.0012 | 0.0139 | 0.0096 | 0.0018 | 0.0245 |
Bud Periphery | 0.0054 | 0.0023 | 0.012 | 0.0059 | 0.0068 | 0.0049 | 0.0102 | 0.0085 | 0.0112 | 0.0674 | 0.022 | 0.0041 | 0.0033 | 0.0049 | 0.0019 | 0.003 | 0.0009 | 0.0071 |
Bud Site | 0.0089 | 0.0714 | 0.0335 | 0.0103 | 0.0427 | 0.0017 | 0.0364 | 0.1604 | 0.047 | 0.0116 | 0.0214 | 0.0028 | 0.0194 | 0.0523 | 0.0198 | 0.0034 | 0.002 | 0.0009 |
Cell Periphery | 0.0259 | 0.0162 | 0.0147 | 0.0057 | 0.0186 | 0.0089 | 0.0107 | 0.0148 | 0.0075 | 0.0044 | 0.0031 | 0.0053 | 0.0051 | 0.0054 | 0.0036 | 0.0014 | 0.0006 | 0.003 |
Cytoplasm | 0.4552 | 0.5924 | 0.5785 | 0.4055 | 0.4185 | 0.614 | 0.6292 | 0.6628 | 0.7009 | 0.6831 | 0.6 | 0.7659 | 0.6732 | 0.7463 | 0.7451 | 0.7673 | 0.806 | 0.792 |
Cytoplasmic Foci | 0.0202 | 0.0077 | 0.0116 | 0.0163 | 0.0289 | 0.0073 | 0.0202 | 0.0053 | 0.0177 | 0.0109 | 0.0363 | 0.0172 | 0.0246 | 0.0064 | 0.0092 | 0.0074 | 0.0243 | 0.0138 |
Eisosomes | 0.0016 | 0.0008 | 0.001 | 0.0006 | 0.0019 | 0.0004 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0096 | 0.0024 | 0.0052 | 0.0051 | 0.0065 | 0.0026 | 0.0073 | 0.0036 | 0.0045 | 0.0062 | 0.0095 | 0.0023 | 0.0045 | 0.0042 | 0.0062 | 0.0051 | 0.004 | 0.0075 |
Endosome | 0.0261 | 0.001 | 0.0021 | 0.0015 | 0.0266 | 0.0021 | 0.036 | 0.0008 | 0.0085 | 0.0045 | 0.0412 | 0.0068 | 0.0085 | 0.0025 | 0.0083 | 0.0065 | 0.046 | 0.0035 |
Golgi | 0.007 | 0.0033 | 0.0033 | 0.0022 | 0.0104 | 0.0013 | 0.011 | 0.0007 | 0.0055 | 0.0022 | 0.0374 | 0.0049 | 0.0025 | 0.001 | 0.0052 | 0.0023 | 0.008 | 0.0014 |
Lipid Particles | 0.0081 | 0.0006 | 0.0033 | 0.0024 | 0.012 | 0.0023 | 0.0099 | 0.0012 | 0.0024 | 0.0004 | 0.0351 | 0.0014 | 0.0056 | 0.0004 | 0.0024 | 0.0019 | 0.0196 | 0.0004 |
Mitochondria | 0.0179 | 0.0023 | 0.0014 | 0.0026 | 0.0048 | 0.0014 | 0.0078 | 0.0002 | 0.0043 | 0.0012 | 0.0135 | 0.007 | 0.0014 | 0.0002 | 0.0019 | 0.0023 | 0.0048 | 0.0005 |
None | 0.3047 | 0.2602 | 0.2948 | 0.4193 | 0.3346 | 0.3091 | 0.1377 | 0.1254 | 0.1285 | 0.141 | 0.0668 | 0.1185 | 0.1945 | 0.1657 | 0.1206 | 0.1447 | 0.0129 | 0.1175 |
Nuclear Periphery | 0.0085 | 0.0002 | 0.0005 | 0.0005 | 0.0068 | 0.0003 | 0.006 | 0.0003 | 0.0008 | 0.0007 | 0.0071 | 0.0003 | 0.0013 | 0.0003 | 0.0017 | 0.0025 | 0.0146 | 0.0003 |
Nucleolus | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0019 | 0.0002 | 0.001 | 0.0002 | 0.0006 | 0.0003 | 0.0145 | 0.0001 | 0.0018 | 0.0001 | 0.0001 | 0.0009 | 0.0014 | 0 |
Nucleus | 0.0082 | 0.0004 | 0.0008 | 0.0007 | 0.0024 | 0.0007 | 0.0034 | 0.001 | 0.0014 | 0.0019 | 0.0065 | 0.0005 | 0.0055 | 0.0006 | 0.0007 | 0.0022 | 0.0033 | 0.0003 |
Peroxisomes | 0.0223 | 0.0053 | 0.0054 | 0.026 | 0.007 | 0.0021 | 0.008 | 0.0001 | 0.0037 | 0.0008 | 0.0173 | 0.0013 | 0.0071 | 0.0001 | 0.0028 | 0.0016 | 0.0029 | 0.0011 |
Punctate Nuclear | 0.0023 | 0.0002 | 0.0012 | 0.001 | 0.0075 | 0.0004 | 0.0022 | 0.0005 | 0.0009 | 0.0007 | 0.0063 | 0.0006 | 0.0096 | 0.0002 | 0.0005 | 0.0055 | 0.0259 | 0.0003 |
Vacuole | 0.0045 | 0.0012 | 0.0014 | 0.0022 | 0.0066 | 0.0009 | 0.0102 | 0.0015 | 0.0029 | 0.0046 | 0.0073 | 0.0011 | 0.0071 | 0.002 | 0.0017 | 0.0011 | 0.0031 | 0.0005 |
Vacuole Periphery | 0.0011 | 0 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0019 | 0.0001 | 0.0003 | 0.0005 | 0.0014 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0003 | 0.0027 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.888 | 10.5458 | 14.2625 | 19.7442 | 15.9121 | 16.3546 | 17.4562 | 19.6169 | 18.2984 | 20.5759 |
Translational Efficiency | 0.25 | 0.2973 | 0.2836 | 0.2309 | 0.1901 | 0.2203 | 0.2238 | 0.1891 | 0.2012 | 0.1783 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2005 | 1523 | 1896 | 1634 | 1891 | 2283 | 1369 | 2096 | 3896 | 3806 | 3265 | 3730 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 617.48 | 746.09 | 1066.97 | 919.66 | 711.03 | 772.54 | 858.24 | 899.16 | 662.89 | 761.96 | 979.45 | 908.14 |
Standard Deviation | 76.58 | 96.68 | 120.10 | 116.65 | 97.96 | 94.46 | 117.36 | 115.47 | 99.30 | 96.23 | 157.35 | 116.43 |
Intensity Change Log 2 | — | 0.272957 | 0.789055 | 0.574708 | — | 0.119699 | 0.271471 | 0.338667 | — | 0.192961 | 0.535207 | 0.453194 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.008302 | 0.003585 | 0.005907 | 0.003570 | 0.002906 | 0.005162 | 0.000663 | 0.006523 | 0.005683 | 0.004531 | 0.003709 | 0.005230 |
Bud Neck | 0.102753 | 0.036487 | 0.013621 | 0.011974 | 0.034527 | 0.057979 | 0.004373 | 0.012430 | 0.069638 | 0.049379 | 0.009743 | 0.012230 |
Bud Site | 0.063080 | 0.047181 | 0.055663 | 0.065894 | 0.022885 | 0.049497 | 0.010100 | 0.075347 | 0.043570 | 0.048570 | 0.036559 | 0.071206 |
Cell Periphery | 0.006800 | 0.000395 | 0.001252 | 0.000663 | 0.000728 | 0.000889 | 0.000377 | 0.000735 | 0.003853 | 0.000691 | 0.000885 | 0.000704 |
Cytoplasm | 0.509238* | 0.475245* | 0.527002* | 0.500243* | 0.518281* | 0.479333* | 0.590385* | 0.546122* | 0.513627* | 0.477697* | 0.553578* | 0.526024* |
Cytoplasmic Foci | 0.102165 | 0.241679* | 0.044498 | 0.051317 | 0.189770* | 0.213125* | 0.059568 | 0.050675 | 0.144686 | 0.224551* | 0.050817 | 0.050956 |
Eisosomes | 0.013134 | 0.000781 | 0.000325 | 0.000206 | 0.001618 | 0.002768 | 0.000148 | 0.000388 | 0.007545 | 0.001973 | 0.000251 | 0.000308 |
Endoplasmic Reticulum | 0.004112 | 0.001460 | 0.004767 | 0.001727 | 0.001927 | 0.001345 | 0.001626 | 0.001753 | 0.003051 | 0.001391 | 0.003450 | 0.001742 |
Endosome | 0.004963 | 0.019345 | 0.002255 | 0.007898 | 0.010636 | 0.019107 | 0.004001 | 0.003565 | 0.007716 | 0.019202 | 0.002987 | 0.005463 |
Golgi | 0.008767 | 0.009480 | 0.000193 | 0.001870 | 0.006655 | 0.017559 | 0.000451 | 0.000685 | 0.007742 | 0.014326 | 0.000301 | 0.001204 |
Lipid Particles | 0.011843 | 0.006655 | 0.002158 | 0.001384 | 0.010220 | 0.007512 | 0.002039 | 0.001404 | 0.011055 | 0.007169 | 0.002108 | 0.001395 |
Mitochondria | 0.011895 | 0.005507 | 0.000224 | 0.001488 | 0.003874 | 0.008112 | 0.000174 | 0.000523 | 0.008002 | 0.007070 | 0.000203 | 0.000946 |
Mitotic Spindle | 0.000527 | 0.001814 | 0.002544 | 0.018196 | 0.002480 | 0.003447 | 0.001383 | 0.009678 | 0.001475 | 0.002793 | 0.002057 | 0.013409 |
None | 0.006052 | 0.001244 | 0.001520 | 0.000861 | 0.003641 | 0.001409 | 0.005067 | 0.000656 | 0.004882 | 0.001343 | 0.003007 | 0.000746 |
Nuclear Periphery | 0.000556 | 0.000156 | 0.000443 | 0.000344 | 0.000310 | 0.000079 | 0.000547 | 0.000198 | 0.000437 | 0.000110 | 0.000487 | 0.000262 |
Nuclear Periphery Foci | 0.000636 | 0.000588 | 0.003471 | 0.001492 | 0.000535 | 0.000279 | 0.002073 | 0.001108 | 0.000587 | 0.000403 | 0.002885 | 0.001277 |
Nucleolus | 0.000867 | 0.000575 | 0.000092 | 0.000270 | 0.000770 | 0.000464 | 0.000343 | 0.000108 | 0.000820 | 0.000508 | 0.000197 | 0.000179 |
Nucleus | 0.104537 | 0.045064 | 0.163175* | 0.069463 | 0.120299 | 0.052653 | 0.173381* | 0.052080 | 0.112187 | 0.049616 | 0.167454* | 0.059695 |
Peroxisomes | 0.013923 | 0.013821 | 0.001479 | 0.003437 | 0.007185 | 0.014908 | 0.001369 | 0.004595 | 0.010652 | 0.014473 | 0.001433 | 0.004088 |
Vacuole | 0.024849 | 0.087240 | 0.169324 | 0.256128* | 0.059880 | 0.062786 | 0.141819 | 0.230778* | 0.041852 | 0.072571 | 0.157791 | 0.241883* |
Vacuole Periphery | 0.001002 | 0.001700 | 0.000086 | 0.001577 | 0.000875 | 0.001587 | 0.000113 | 0.000649 | 0.000940 | 0.001632 | 0.000097 | 0.001055 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 4.85 | 2.80 | 5.65 | 1.39 | 3.24 | -2.70 | 3.52 | -2.77 | -0.00 | -8.15 | 1.74 | 3.54 | 2.41 | 0.80 | -1.53 |
Bud Neck | 15.10 | 22.47 | 22.84 | 12.60 | 1.67 | -8.38 | 16.13 | 12.61 | 21.34 | -6.79 | 7.44 | 26.00 | 25.32 | 24.64 | -1.81 |
Bud Site | 4.17 | 4.37 | 3.71 | -0.18 | -0.47 | -10.16 | 6.61 | -10.38 | -2.08 | -15.09 | -2.02 | 5.39 | -3.38 | -1.66 | -8.37 |
Cell Periphery | 12.10 | 10.70 | 11.87 | -1.76 | 5.77 | -2.54 | 7.60 | 2.48 | 5.10 | -4.55 | 11.35 | 10.93 | 11.87 | 3.39 | 3.86 |
Cytoplasm | 3.98 | 5.39 | 7.80 | 3.68 | 2.70 | 5.14 | -2.48 | 2.77 | -1.94 | 4.66 | 6.37 | 2.75 | 7.08 | 1.14 | 3.84 |
Cytoplasmic Foci | -23.95 | 19.45 | 16.73 | 35.32 | -2.01 | -4.20 | 28.77 | 34.11 | 40.22 | 4.03 | -20.13 | 34.33 | 35.91 | 53.38 | 1.14 |
Eisosomes | 15.13 | 15.76 | 15.89 | 9.87 | 5.62 | -5.64 | 13.11 | 11.52 | 14.24 | -6.77 | 12.52 | 16.89 | 16.83 | 15.97 | -1.37 |
Endoplasmic Reticulum | 4.49 | -2.45 | 3.94 | -1.75 | 13.50 | 2.35 | -0.51 | 0.76 | -2.05 | 1.70 | 5.08 | -3.11 | 3.96 | -2.74 | 12.82 |
Endosome | -12.04 | 9.45 | -0.73 | 10.38 | -4.88 | -7.50 | 9.32 | 10.93 | 17.65 | 3.43 | -14.39 | 12.57 | 7.86 | 19.60 | -2.97 |
Golgi | -0.81 | 18.38 | 12.43 | 11.61 | -2.09 | -11.89 | 13.31 | 14.60 | 20.76 | -0.43 | -10.35 | 22.83 | 19.22 | 23.42 | -2.21 |
Lipid Particles | 6.28 | 13.81 | 15.08 | 10.32 | 7.30 | 3.65 | 12.23 | 13.22 | 14.75 | 4.70 | 7.00 | 18.39 | 20.06 | 17.96 | 8.76 |
Mitochondria | 5.93 | 13.82 | 12.40 | 6.20 | -2.31 | -5.27 | 9.84 | 9.04 | 10.77 | -4.20 | 1.39 | 16.40 | 14.99 | 12.29 | -3.31 |
Mitotic Spindle | -1.70 | -2.56 | -6.57 | -5.59 | -5.24 | -0.71 | 1.02 | -3.62 | -3.15 | -4.80 | -1.71 | -0.86 | -7.37 | -6.10 | -6.87 |
None | 6.14 | 6.03 | 6.90 | 1.80 | 1.57 | 3.17 | -0.52 | 4.40 | 3.85 | 4.53 | 6.70 | 3.85 | 8.14 | 4.11 | 4.84 |
Nuclear Periphery | 1.67 | -0.34 | 0.23 | -8.13 | 4.22 | 2.46 | -3.55 | 0.14 | -9.86 | 12.39 | 2.50 | -1.58 | 0.38 | -12.76 | 11.87 |
Nuclear Periphery Foci | 0.14 | -10.69 | -4.12 | -7.93 | 10.05 | 2.90 | -10.68 | -4.93 | -10.05 | 7.40 | 1.40 | -14.66 | -5.48 | -12.81 | 13.13 |
Nucleolus | 1.32 | 3.71 | 2.49 | 2.16 | -2.46 | 2.54 | 4.51 | 7.35 | 4.02 | 4.42 | 2.43 | 5.40 | 5.13 | 4.21 | -0.17 |
Nucleus | 17.54 | -14.10 | 7.46 | -9.49 | 20.66 | 19.25 | -9.64 | 16.81 | -3.01 | 21.84 | 25.54 | -16.72 | 17.32 | -8.64 | 31.07 |
Peroxisomes | 0.07 | 14.06 | 11.36 | 11.37 | -3.57 | -8.50 | 9.89 | 5.25 | 13.06 | -3.84 | -4.97 | 17.17 | 12.09 | 17.40 | -5.27 |
Vacuole | -21.13 | -37.53 | -44.44 | -33.58 | -12.36 | -1.18 | -20.20 | -40.24 | -39.74 | -13.46 | -16.46 | -40.86 | -60.27 | -52.03 | -17.38 |
Vacuole Periphery | -3.24 | 8.74 | 0.56 | 2.73 | -3.19 | -3.72 | 5.76 | 2.20 | 5.73 | -2.31 | -4.94 | 10.06 | 2.01 | 5.78 | -3.97 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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