Standard name
Human Ortholog
Description Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.08 0.07 0 0.09 0.08 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.96 0.97 0.85 0.79 0.66 0.59 0.56 0.51 0.49 0.38 0.41 0.96 0.97 0.93 0.92 0.95 0.93 0.77 0.87 0.67 0.88 0.77 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0.07 0.08 0.45 0.44 0.41 0.56 0.56 0.74 0.65 0 0 0 0 0 0 0.1 0 0.17 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.08 0.09 0.06 0.1 0.14 0.12 0.12 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 2 0 0 0 0 0 1 1 1 0 0 0 4 2 5 0 2 2
Bud 1 2 2 4 16 22 16 26 24 22 21 14 1 0 0 3 0 1 1 1 1 2 7 8
Bud Neck 1 1 0 0 4 4 5 2 3 6 1 1 7 10 10 1 1 2 2 0 1 1 2 1
Bud Site 0 0 0 0 0 0 2 2 0 4 6 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 0 1 1 0 1 1 0 1 3 1 1 4 13 10 6 2 0 1 1 0 2
Cytoplasm 336 231 266 107 154 203 215 161 154 132 105 130 392 518 458 349 249 305 279 209 186 273 210 299
Endoplasmic Reticulum 1 3 1 0 0 0 0 0 0 1 0 1 3 2 5 16 18 28 2 1 3 0 1 2
Endosome 0 0 2 4 10 4 7 9 8 4 3 7 3 1 5 13 2 6 9 4 14 6 10 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 3 0 0 2 0 0 0
Mitochondria 41 8 2 9 16 139 161 118 168 150 204 206 0 0 2 3 6 9 36 9 47 11 6 19
Nucleus 0 0 0 3 1 0 2 1 1 0 1 1 0 2 0 2 0 0 0 0 0 1 4 0
Nuclear Periphery 1 0 0 2 0 0 0 0 0 3 0 2 0 0 0 0 0 1 0 0 0 0 2 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 1 3 6 3 3 7 3 7 6 12 0 0 1 1 0 1 0 0 0 0 0 0
SpindlePole 4 1 4 10 17 19 37 39 36 33 21 28 7 12 20 3 2 6 8 2 7 4 6 13
Vac/Vac Membrane 0 0 1 0 1 2 3 0 2 1 6 4 1 2 2 8 6 7 5 2 1 3 8 4
Unique Cell Count 364 240 275 126 194 308 366 287 302 270 276 316 407 535 495 378 262 329 363 242 280 312 274 370
Labelled Cell Count 386 246 281 142 227 397 453 367 400 363 375 414 416 549 508 414 296 375 363 242 280 312 274 370


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 3.8 3.9 3.7 3.8 3.0 3.1 3.3 3.0 3.0 2.8 2.7 5.3 5.4 5.5 6.2 5.6 5.5 5.5 6.1 6.1
Std Deviation (1e-4) 0.4 0.6 1.4 1.7 1.8 1.1 1.4 1.8 1.0 1.2 0.9 0.7 1.0 1.2 1.4 1.8 1.6 1.8 1.0 1.8 1.7
Intensity Change (Log2) – – – -0.07 -0.06 -0.37 -0.32 -0.26 -0.38 -0.4 -0.51 -0.52 0.44 0.46 0.48 0.65 0.51 0.49 0.5 0.63 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6084 0.1232 -0.1818 -0.431 -0.3608 -0.0126 0.8304 0.2855 0.1235 0.254 0.2508 0.0362 -0.8129 -0.722 -0.6844 -0.5577 -1.2324 -0.7332
Actin 0.0006 0.0006 0.0002 0.0022 0.0004 0.0006 0.0433 0.0028 0.0041 0.0596 0.0038 0.0018 0.024 0.0016 0.0012 0.0003 0.0014 0.0001
Bud 0.0003 0.0007 0.0002 0.0001 0.0015 0.0002 0.0022 0.0018 0.001 0.0007 0.0004 0.0016 0.0006 0.0066 0.0002 0.0094 0.0077 0
Bud Neck 0.0002 0.0003 0 0.0001 0.0004 0.0003 0.0043 0.0002 0.0012 0.0155 0.0015 0.0011 0.0022 0.0001 0.0004 0.0002 0.0004 0.0001
Bud Periphery 0.0004 0.0007 0.0004 0.0002 0.0025 0.0003 0.0049 0.0023 0.0051 0.0014 0.0003 0.0044 0.0006 0.0047 0.0001 0.0025 0.0064 0
Bud Site 0.0014 0.01 0.0007 0 0.0007 0.0001 0.0114 0.0039 0.0006 0.0018 0.0047 0.0007 0.0032 0.0027 0.0022 0.0016 0.0014 0
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0009 0.0002 0.0001 0.0002 0.0001 0.0001 0.0005 0.0002 0.0002 0.0003 0.0002 0
Cytoplasm 0.022 0.0238 0.0407 0.0404 0.0364 0.0395 0.0395 0.0676 0.0852 0.0518 0.0585 0.0833 0.0268 0.0674 0.0419 0.0423 0.036 0.0811
Cytoplasmic Foci 0.0168 0.015 0.0187 0.0443 0.0303 0.0172 0.0609 0.0051 0.012 0.033 0.0454 0.0167 0.0095 0.0072 0.0045 0.0521 0.0137 0.0193
Eisosomes 0.0003 0.0004 0.0002 0.0004 0.0001 0.0001 0.0005 0.0001 0.0001 0.0003 0.0001 0.0001 0.0005 0.0001 0.0003 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0007 0.0004 0.0004 0.0005 0.0002 0.0002 0.0036 0.0003 0.0029 0.0021 0.0013 0.0006 0.002 0.0004 0.0016 0.0007 0.0012 0.0005
Endosome 0.0044 0.0017 0.0053 0.0042 0.0067 0.0029 0.0544 0.0007 0.02 0.0378 0.0598 0.0131 0.0107 0.0031 0.001 0.0065 0.0003 0.0085
Golgi 0.0004 0.0003 0.0002 0.0003 0.0013 0.0001 0.0195 0.0006 0.0126 0.0115 0.0169 0.0039 0.0034 0.0001 0.0001 0.0006 0.0002 0.0003
Lipid Particles 0.0016 0.0006 0.0005 0.0027 0.0004 0.0003 0.0387 0.002 0.0041 0.0284 0.0228 0.0026 0.0045 0.0003 0.0007 0.0035 0.0008 0.0004
Mitochondria 0.0003 0.0003 0.0003 0.0006 0.0197 0.0003 0.0127 0.0007 0.0056 0.0352 0.0026 0.0042 0.0016 0.0006 0.0004 0.001 0.0015 0.0002
None 0.9413 0.9406 0.9294 0.8972 0.8911 0.9355 0.6214 0.9032 0.8347 0.693 0.7625 0.8478 0.8788 0.9026 0.9328 0.8551 0.9057 0.8867
Nuclear Periphery 0.0044 0.0002 0.0003 0.0008 0.0001 0.0001 0.0063 0.0003 0.0024 0.0054 0.0014 0.0005 0.0114 0.0002 0.004 0.0009 0.0017 0.0002
Nucleolus 0.0002 0.0006 0.0001 0.0002 0.0003 0.0001 0.0018 0.0004 0.0003 0.0003 0.0004 0.0002 0.0011 0.0003 0.0004 0.0015 0.0013 0.0001
Nucleus 0.0011 0.0008 0.0005 0.0005 0.0003 0.0003 0.0023 0.001 0.0009 0.0005 0.001 0.0008 0.0077 0.0006 0.0056 0.0029 0.003 0.0003
Peroxisomes 0.0012 0.0007 0.0003 0.0022 0.0054 0.0005 0.0538 0.0034 0.0028 0.0151 0.01 0.0065 0.0015 0.0001 0.0001 0.0019 0.0007 0.0003
Punctate Nuclear 0.0019 0.0016 0.0007 0.0019 0.0012 0.0008 0.0102 0.0028 0.0009 0.0041 0.0041 0.0015 0.0075 0.0004 0.0016 0.015 0.0151 0.001
Vacuole 0.0003 0.0005 0.0006 0.0009 0.0006 0.0002 0.0055 0.0004 0.0023 0.0011 0.0019 0.0066 0.0012 0.0008 0.0007 0.0011 0.0009 0.0005
Vacuole Periphery 0.0001 0 0.0001 0.0002 0.0001 0.0001 0.0019 0.0001 0.001 0.001 0.0006 0.002 0.0007 0.0001 0.0002 0.0003 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.6397 2.3598 4.6694 3.1226 3.4225 5.2765 4.1754 4.5627 3.0822 5.8008
Translational Efficiency 1.1822 1.5508 0.5308 0.8339 1.3009 1.0048 0.8527 0.6188 1.1843 0.632

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells
Localization
Cell Percentages cytoplasm (31%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Atg17

Atg17


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available