Standard name
Human Ortholog
Description Protein that binds to the Rap1p C-terminus; acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation; RIF2 has a paralog, ORC4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.16 0.14 0.32 0.3 0.23 0.17 0.15 0.21 0.13 0.15 0.16 0.1 0.25 0.29 0.41 0.35 0.33 0.34 0.1 0.12 0.15 0.22 0.19 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.66 0.26 0.15 0.22 0.39 0.69 0.75 0.58 0.62 0.7 0.67 0.78 0 0 0 0 0.09 0 0 0 0.1 0.05 0.06 0.05
Nucleus 0.44 0.5 0.33 0.38 0.29 0.31 0.32 0.32 0.36 0.3 0.28 0.28 0.69 0.65 0.51 0.42 0.31 0.37 0.49 0.52 0.32 0.35 0.29 0.28
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.29 0.2 0.18 0.22 0.12 0.08 0.12 0.09 0.1 0.13 0.09 0.05 0.08 0.09 0.26 0.33 0.33 0.27 0.22 0.25 0.29 0.33 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0
Bud 0 2 1 4 7 16 12 17 13 13 12 11 0 0 0 0 2 0 0 7 10 3 5 6
Bud Neck 0 1 0 0 1 0 0 1 1 0 0 0 2 1 0 0 0 0 0 0 2 2 1 3
Bud Site 0 1 0 0 2 6 8 5 7 11 13 14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 26 36 85 67 59 78 75 136 61 79 77 56 53 68 101 73 137 110 19 42 62 47 56 68
Endoplasmic Reticulum 1 2 2 0 1 0 0 1 0 0 0 1 1 1 0 10 19 22 0 0 3 0 0 3
Endosome 0 0 20 12 10 3 3 13 6 9 10 2 3 3 8 3 18 14 1 5 13 0 6 8
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 1 1 2 0 1 1
Mitochondria 108 68 41 48 99 323 380 378 282 365 332 447 2 1 0 2 37 9 6 7 41 10 16 18
Nucleus 72 129 88 84 73 144 165 207 161 158 138 164 145 155 125 88 130 121 93 184 131 76 85 93
Nuclear Periphery 1 0 2 2 2 4 1 2 9 10 4 15 0 0 0 0 0 0 2 1 6 0 0 2
Nucleolus 0 0 1 1 1 1 1 4 4 2 6 1 0 0 0 0 0 0 1 1 3 0 1 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1
SpindlePole 0 1 0 4 2 3 2 3 10 5 2 4 2 2 2 1 5 1 7 12 14 8 11 13
Vac/Vac Membrane 21 74 54 39 55 57 39 77 42 55 65 51 10 20 22 54 138 109 51 77 104 64 95 108
Unique Cell Count 163 258 265 221 253 471 509 653 452 525 495 576 209 238 247 208 415 327 189 352 414 221 293 339
Labelled Cell Count 229 314 294 261 313 635 686 845 596 707 660 767 218 251 260 231 488 388 189 352 414 221 293 339


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.2 4.8 4.4 4.3 3.4 3.4 3.6 3.4 3.4 3.3 3.1 5.6 5.6 5.3 5.9 5.5 5.5 5.8 6.0 6.1
Std Deviation (1e-4) 0.5 0.6 2.3 2.1 1.9 1.2 1.4 1.8 0.8 0.7 1.1 0.5 1.0 1.0 1.0 1.5 1.2 1.3 1.5 1.4 1.2
Intensity Change (Log2) – – – -0.13 -0.18 -0.5 -0.5 -0.45 -0.52 -0.53 -0.57 -0.65 0.21 0.2 0.13 0.29 0.18 0.18 0.26 0.3 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.4 3.6 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9415 1.0479 0.8857 1.0334 1.2634 0.776 1.562 1.3729 1.3812 0.9395 2.4262 1.1495 0.9574 0.3271 0.1617 0.9894 0.5251 0.345
Actin 0.0134 0.0005 0.0048 0.0245 0.052 0.0006 0.0106 0.0002 0.0047 0.0062 0.0254 0.0023 0.023 0.0014 0.0105 0.0097 0.0129 0.0042
Bud 0.0007 0.0001 0.0001 0.0002 0.0003 0.0009 0.0008 0.0001 0.0003 0.0004 0.0005 0.0001 0.0015 0.0001 0.0001 0.0017 0.0121 0.0004
Bud Neck 0.0064 0.0008 0.0004 0.0011 0.0032 0.0025 0.0051 0.0004 0.0049 0.001 0.0114 0.0017 0.0106 0.0007 0.0007 0.0031 0.0054 0.0025
Bud Periphery 0.001 0.0001 0.0002 0.0002 0.0005 0.0003 0.0023 0.0001 0.0007 0.0011 0.0015 0.0001 0.0032 0.0001 0.0001 0.0063 0.002 0.0003
Bud Site 0.0021 0.0016 0.0005 0.0051 0.0013 0.0005 0.0026 0.0007 0.0024 0.0005 0.0016 0.0001 0.0057 0.0005 0.0002 0.0115 0.0066 0.0014
Cell Periphery 0.0002 0.0001 0 0.0002 0.0002 0 0.0004 0 0.0001 0.0001 0.0004 0 0.0003 0 0 0.0004 0.0003 0
Cytoplasm 0.0411 0.0608 0.0401 0.0683 0.0115 0.0446 0.0512 0.0715 0.0437 0.0268 0.0488 0.0356 0.0394 0.0475 0.0414 0.0362 0.0348 0.0237
Cytoplasmic Foci 0.0123 0.0045 0.0045 0.0094 0.0149 0.0027 0.0073 0.0018 0.0028 0.0026 0.0125 0.0021 0.0127 0.0008 0.0018 0.0026 0.0102 0.0019
Eisosomes 0.0004 0.0001 0.0002 0.0002 0.0007 0 0.0002 0 0.0001 0.0001 0.0005 0 0.0002 0 0.0001 0.0003 0.0002 0
Endoplasmic Reticulum 0.0026 0.0028 0.0017 0.006 0.0018 0.0008 0.0037 0.0021 0.0031 0.002 0.005 0.0016 0.0055 0.0025 0.0012 0.0022 0.0031 0.0011
Endosome 0.014 0.0026 0.0042 0.0165 0.0081 0.0019 0.0157 0.0017 0.0048 0.0118 0.0247 0.0032 0.0196 0.0019 0.0022 0.0064 0.0066 0.0031
Golgi 0.0021 0.0001 0.0012 0.0038 0.0083 0.0001 0.0029 0.0001 0.0011 0.0055 0.0083 0.0004 0.0048 0.0002 0.0014 0.0024 0.0023 0.0013
Lipid Particles 0.0055 0.0004 0.0027 0.0045 0.0142 0.0003 0.0134 0.0001 0.002 0.0102 0.0248 0.0017 0.011 0.0002 0.008 0.0026 0.0036 0.0027
Mitochondria 0.0024 0.0011 0.0023 0.0018 0.0046 0.0005 0.011 0.0005 0.0013 0.0153 0.0181 0.0008 0.0072 0.0008 0.0018 0.0127 0.003 0.0015
None 0.0821 0.1237 0.1335 0.1075 0.0668 0.09 0.0353 0.0979 0.0349 0.0729 0.111 0.0274 0.0768 0.0712 0.1026 0.0947 0.0555 0.0478
Nuclear Periphery 0.0433 0.0499 0.0448 0.0669 0.0212 0.022 0.0341 0.0208 0.0339 0.0133 0.0837 0.0185 0.0409 0.0456 0.0399 0.0175 0.0743 0.0169
Nucleolus 0.0085 0.0097 0.0075 0.0078 0.0264 0.0122 0.0083 0.0068 0.0046 0.0059 0.0086 0.0086 0.0095 0.0063 0.0054 0.0155 0.0096 0.0101
Nucleus 0.6771 0.6569 0.6727 0.5056 0.6324 0.6978 0.7145 0.7296 0.8042 0.7682 0.5144 0.8019 0.6509 0.7521 0.722 0.7209 0.7231 0.8185
Peroxisomes 0.0037 0.0002 0.004 0.0017 0.0117 0.0005 0.0046 0.0001 0.001 0.0029 0.0125 0.0016 0.0056 0.0001 0.0005 0.0013 0.0037 0.0074
Punctate Nuclear 0.0786 0.082 0.0731 0.1666 0.1177 0.1208 0.0702 0.0638 0.0471 0.0511 0.0772 0.0911 0.0659 0.0657 0.0592 0.0473 0.0281 0.0543
Vacuole 0.0014 0.0015 0.0008 0.0012 0.001 0.0004 0.0037 0.0012 0.0016 0.0012 0.005 0.001 0.0036 0.0014 0.0005 0.0032 0.0015 0.0005
Vacuole Periphery 0.001 0.0006 0.0005 0.0007 0.001 0.0003 0.0019 0.0003 0.0009 0.0009 0.0042 0.0003 0.0019 0.0007 0.0005 0.0015 0.0012 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.0371 8.3028 3.0805 5.3404 6.6232 4.6414 5.2469 6.2327 3.5492 5.9255
Translational Efficiency 0.4821 0.3315 0.6885 0.3848 0.3353 0.9275 0.5936 0.6446 0.7446 0.6372

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1372 183 2413 1351 1157 1362 293 940 2529 1545 2706 2291

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 644.83 774.21 758.82 802.14 669.02 708.36 879.75 817.99 655.90 716.16 771.91 808.64
Standard Deviation 86.67 110.64 86.97 120.28 88.29 88.57 106.00 116.64 88.24 93.90 96.81 119.06
Intensity Change Log 2 — 0.263806 0.234839 0.314935 — 0.082433 0.395044 0.290034 — 0.174300 0.318637 0.302309

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000084 0.000775 0.000173 0.000600 0.000146 0.000200 0.000413 0.000538 0.000112 0.000268 0.000199 0.000575
Bud Neck 0.003192 0.009357 0.011981 0.014515 0.002888 0.009246 0.002554 0.010264 0.003053 0.009259 0.010961 0.012771
Bud Site 0.000931 0.004399 0.008876 0.017578 0.001946 0.005417 0.000822 0.021276 0.001395 0.005296 0.008004 0.019095
Cell Periphery 0.000120 0.000135 0.000264 0.000238 0.000124 0.000317 0.000089 0.000288 0.000122 0.000295 0.000245 0.000259
Cytoplasm 0.208328* 0.232372* 0.143108* 0.133591* 0.220204* 0.246253* 0.035575 0.180339* 0.213761* 0.244609* 0.131465* 0.152772*
Cytoplasmic Foci 0.047837 0.072984 0.002639 0.005544 0.046657 0.073845 0.003714 0.004175 0.047297 0.073743 0.002755 0.004982
Eisosomes 0.000047 0.000081 0.000025 0.000036 0.000047 0.000066 0.000049 0.000039 0.000047 0.000068 0.000027 0.000038
Endoplasmic Reticulum 0.001915 0.005970 0.002149 0.007481 0.001630 0.001408 0.007458 0.005984 0.001785 0.001949 0.002724 0.006867
Endosome 0.002118 0.005337 0.000874 0.007541 0.002294 0.005260 0.000973 0.002870 0.002199 0.005269 0.000885 0.005624
Golgi 0.001011 0.000408 0.000048 0.005352 0.000645 0.001401 0.000046 0.000427 0.000844 0.001284 0.000048 0.003331
Lipid Particles 0.006141 0.006285 0.000516 0.001009 0.005846 0.007656 0.001376 0.001210 0.006006 0.007494 0.000609 0.001091
Mitochondria 0.003560 0.002584 0.002161 0.005418 0.002644 0.007688 0.000497 0.002938 0.003141 0.007083 0.001980 0.004401
Mitotic Spindle 0.003741 0.012615 0.006414 0.028540 0.000807 0.004198 0.000222 0.019284 0.002399 0.005195 0.005743 0.024742
None 0.013950 0.009296 0.007402 0.007670 0.008660 0.009220 0.004246 0.013320 0.011530 0.009229 0.007060 0.009988
Nuclear Periphery 0.003113 0.002594 0.002152 0.003403 0.002439 0.002014 0.001709 0.002581 0.002805 0.002082 0.002104 0.003066
Nuclear Periphery Foci 0.001212 0.001807 0.000187 0.001008 0.000960 0.000547 0.000689 0.002520 0.001097 0.000696 0.000241 0.001628
Nucleolus 0.005323 0.004907 0.001312 0.001876 0.003947 0.006364 0.001622 0.001630 0.004694 0.006191 0.001346 0.001775
Nucleus 0.688492* 0.588292* 0.799190* 0.707733* 0.687010* 0.598148* 0.918243* 0.690411* 0.687814* 0.596981* 0.812080* 0.700626*
Peroxisomes 0.002526 0.005677 0.000139 0.000492 0.003494 0.005163 0.000244 0.000370 0.002969 0.005224 0.000150 0.000442
Vacuole 0.005904 0.033282 0.009936 0.043821 0.007067 0.014572 0.019351 0.036533 0.006436 0.016788 0.010955 0.040831
Vacuole Periphery 0.000456 0.000842 0.000454 0.006556 0.000544 0.001016 0.000108 0.003001 0.000496 0.000996 0.000417 0.005097

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.27 -5.88 -4.09 0.71 -3.32 -0.90 -5.63 -5.17 -4.87 -1.23 -3.27 -4.33 -5.97 -3.93 -4.78
Bud Neck -2.75 -8.59 -9.37 -1.53 -1.05 -5.28 -2.19 -6.49 -1.70 -5.10 -6.00 -9.12 -11.35 -3.03 -1.34
Bud Site -4.03 -6.99 -7.31 -4.32 -2.48 -2.83 0.10 -4.94 -3.56 -5.25 -4.49 -6.12 -8.51 -5.22 -3.68
Cell Periphery -1.42 -4.00 -5.11 -3.36 1.03 -4.18 0.92 -2.65 1.74 -2.95 -4.33 -3.86 -4.84 2.15 0.23
Cytoplasm -1.10 10.43 11.52 5.96 2.21 -2.52 21.26 9.40 12.53 -12.22 -3.78 15.82 14.97 16.53 -0.59
Cytoplasmic Foci -2.20 15.70 15.26 6.92 -1.84 -6.07 13.47 14.24 19.31 -0.49 -6.53 21.57 20.87 20.80 -2.27
Eisosomes -3.74 8.36 -0.40 3.67 -10.94 -4.72 -3.11 -4.93 0.24 -0.15 -5.80 8.28 -3.66 3.54 -12.64
Endoplasmic Reticulum -4.94 -3.16 -15.19 -2.72 -13.96 0.96 -10.70 -13.18 -14.17 0.82 -1.37 -7.80 -20.17 -19.35 -15.13
Endosome -2.19 3.70 -2.44 0.77 -4.52 -4.18 2.26 -0.10 4.36 -2.93 -4.90 4.94 -2.30 2.62 -5.52
Golgi 1.50 2.68 -1.24 -1.90 -2.33 -1.51 2.34 1.60 2.62 -2.64 -1.02 3.52 -0.98 -0.19 -2.46
Lipid Particles -0.28 8.12 7.26 4.68 -7.93 -1.67 5.39 5.57 9.48 0.27 -1.79 10.31 9.13 10.68 -7.69
Mitochondria 1.01 1.65 0.09 -1.19 -2.30 -3.65 3.14 0.78 4.23 -2.30 -3.29 1.98 0.44 3.70 -1.91
Mitotic Spindle -1.60 -1.72 -5.84 -0.67 -5.04 -2.46 0.79 -5.37 -4.07 -5.50 -1.98 -2.90 -8.00 -5.71 -6.18
None 2.58 5.02 5.20 1.06 0.35 -0.44 4.00 0.15 0.55 -3.38 2.04 5.18 4.29 1.91 -1.04
Nuclear Periphery 0.44 0.84 -4.12 -5.27 -9.42 0.72 -0.27 -2.81 -6.55 -4.48 1.80 0.25 -5.01 -11.36 -9.86
Nuclear Periphery Foci -2.12 4.33 -0.45 2.04 -8.91 1.72 -0.76 -1.46 -2.45 -0.46 1.70 4.29 -1.51 -3.04 -5.04
Nucleolus 0.39 7.48 5.45 2.71 -1.07 -2.09 2.34 3.29 5.77 0.57 -1.70 7.31 6.07 5.92 -0.94
Nucleus 4.57 -13.12 -2.46 -5.73 10.26 7.33 -14.36 -0.29 -7.33 13.62 9.25 -17.48 -2.10 -10.92 14.84
Peroxisomes -2.40 6.48 5.49 4.31 -1.87 -1.72 3.50 3.42 7.58 -0.53 -3.08 6.26 5.68 8.36 -2.32
Vacuole -5.63 -6.31 -17.42 -5.04 -15.25 -6.15 -5.81 -15.30 -13.69 -5.17 -8.82 -8.36 -23.11 -18.52 -18.88
Vacuole Periphery -1.04 0.28 -3.07 -2.51 -3.13 -2.21 2.77 -0.98 -0.29 -1.66 -2.94 0.74 -3.13 -2.20 -3.31
Standard name
Human Ortholog
Description Protein that binds to the Rap1p C-terminus; acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation; RIF2 has a paralog, ORC4, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (60%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rif2

Rif2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rif2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available