Standard name
Human Ortholog
Description tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.99 0.99 0.97 0.93 0.87 0.87 0.79 0.83 0.75 0.75 0.98 0.98 1.0 0.97 0.94 0.93 0.95 0.95 0.91 0.89 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.13 0.17 0.11 0.38 0.27 0.38 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Bud 0 3 0 1 2 2 1 2 8 7 13 2 0 1 0 0 0 0 0 0 1 1 1 2
Bud Neck 0 0 0 0 1 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 9 6 8 4 7 7 3 8 4 7 2 0 1 3 11 3 0 0 0 0 0 0
Cytoplasm 95 162 285 230 413 364 403 436 351 346 336 336 157 221 257 129 131 155 104 169 268 143 228 218
Endoplasmic Reticulum 0 0 0 3 0 0 0 0 0 0 0 0 1 0 0 9 8 10 0 0 2 1 0 1
Endosome 0 0 0 0 1 4 2 1 6 3 3 3 0 2 0 2 2 3 0 0 2 0 0 2
Golgi 0 0 0 0 0 0 0 1 0 0 1 0 1 1 0 0 2 1 0 1 0 0 1 0
Mitochondria 2 10 1 10 5 50 78 56 169 114 169 142 0 0 0 5 2 3 2 2 6 2 1 2
Nucleus 2 0 0 0 2 0 2 3 1 2 3 1 0 0 1 0 0 2 0 0 0 0 2 3
Nuclear Periphery 0 0 0 0 1 0 0 2 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 2 13 2 1 3 3 0 1 1 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 5 0 2 0 1 3 0 0 0 1 1 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 5 3 11 10 4 15 13 7 9 16 1 0 0 4 3 5 1 0 2 4 3 0
Unique Cell Count 97 167 289 233 427 391 461 503 444 417 448 449 160 225 258 133 139 166 110 178 295 161 244 238
Labelled Cell Count 100 177 300 253 446 435 506 540 556 490 542 516 162 227 260 153 160 182 110 178 295 161 244 238


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 4.5 6.9 6.3 6.7 5.4 5.5 6.1 4.6 4.7 4.8 4.9 6.5 6.4 6.4 6.4 7.9 8.3 7.8 7.7 7.7
Std Deviation (1e-4) 0.7 0.6 1.6 1.3 1.6 1.3 2.5 4.6 1.8 1.3 2.0 1.9 1.6 1.5 1.5 1.4 1.5 1.6 2.5 2.2 2.3
Intensity Change (Log2) – – – -0.12 -0.04 -0.34 -0.33 -0.16 -0.58 -0.55 -0.52 -0.5 -0.09 -0.1 -0.09 -0.1 0.2 0.27 0.18 0.16 0.17

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6008 5.8549 5.1885 4.5196 5.2643 5.2659 4.5311 5.3686 5.2454 4.2101 4.6515 5.2815 1.8258 3.0407 2.6653 2.6131 2.2738 2.4976
Actin 0.0228 0.0016 0.0089 0.0684 0.0064 0.0161 0.058 0.0002 0.0254 0.0005 0.001 0.0195 0.0353 0.0002 0.0219 0.0014 0.0047 0.0001
Bud 0.0006 0.0011 0.0002 0.0004 0.0003 0.0016 0.0008 0.0001 0.0003 0.0002 0.0003 0.0001 0.0016 0.0001 0.0007 0.0003 0.0004 0
Bud Neck 0.0008 0.0002 0.001 0.0132 0.0005 0.0011 0.0005 0.0001 0.0002 0.0002 0.0004 0.0004 0.0061 0.0001 0.0005 0.0006 0.0008 0.0006
Bud Periphery 0.0008 0.0012 0.0002 0.0003 0.0008 0.0053 0.0018 0 0.0001 0.0001 0.0002 0.0001 0.0017 0 0.0006 0.0002 0.0007 0
Bud Site 0.0026 0.009 0.0015 0.0021 0.0013 0.0004 0.0031 0.0003 0.0014 0.0002 0.001 0.0001 0.0032 0.0004 0.0017 0.0048 0.0007 0
Cell Periphery 0.0004 0.0005 0.0006 0.0002 0.0004 0.0004 0.0015 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0002 0.0003 0.0001 0.0001 0
Cytoplasm 0.7895 0.8437 0.884 0.7166 0.6958 0.8532 0.5232 0.9413 0.7822 0.958 0.8034 0.8558 0.6858 0.9713 0.8553 0.8919 0.8111 0.9638
Cytoplasmic Foci 0.0219 0.007 0.0161 0.0419 0.0256 0.0148 0.0406 0.0029 0.0103 0.0047 0.027 0.004 0.0681 0.002 0.0137 0.0179 0.0303 0.0034
Eisosomes 0.0003 0.0001 0.0001 0.0002 0.0002 0 0.0012 0.0001 0.0001 0 0 0.0001 0.0004 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0105 0.0027 0.0069 0.0034 0.0035 0.0062 0.0068 0.0027 0.0027 0.0034 0.0013 0.0072 0.0123 0.002 0.0038 0.0039 0.0022 0.0019
Endosome 0.0283 0.0062 0.0071 0.0231 0.0467 0.0111 0.0442 0.0013 0.0164 0.0032 0.0454 0.003 0.0468 0.0008 0.0145 0.0054 0.0418 0.0023
Golgi 0.0101 0.0007 0.006 0.0057 0.0144 0.0022 0.0406 0.0003 0.0143 0.0002 0.0362 0.0024 0.0181 0.0001 0.0135 0.0024 0.0147 0.0001
Lipid Particles 0.0051 0.0015 0.0014 0.0116 0.0127 0.0005 0.0316 0.0001 0.0035 0.0001 0.0056 0.0011 0.0053 0.0001 0.0074 0.0079 0.0251 0
Mitochondria 0.0144 0.0004 0.0037 0.0275 0.0035 0.0009 0.0487 0.0001 0.001 0.0001 0.0062 0.0005 0.0038 0 0.0099 0.0024 0.0028 0
None 0.0739 0.1043 0.0538 0.0367 0.174 0.0824 0.1399 0.0492 0.1335 0.026 0.0622 0.1035 0.053 0.0215 0.0284 0.0403 0.0084 0.0262
Nuclear Periphery 0.0016 0.0004 0.0007 0.0008 0.0018 0.0004 0.005 0.0003 0.0005 0.0005 0.0004 0.0003 0.0106 0.0002 0.0066 0.0004 0.0258 0.0002
Nucleolus 0.0004 0.0001 0.0001 0.0001 0.0007 0 0.0022 0 0 0 0.0001 0 0.0004 0 0.0002 0.0003 0.0034 0
Nucleus 0.0013 0.0005 0.0005 0.001 0.0012 0.0006 0.0036 0.0003 0.0005 0.0006 0.0006 0.0004 0.0124 0.0004 0.0049 0.0007 0.0071 0.0004
Peroxisomes 0.0037 0.0177 0.0056 0.0417 0.0049 0.0006 0.0229 0 0.0056 0 0.0058 0.0008 0.0165 0 0.0114 0.0158 0.0042 0
Punctate Nuclear 0.0086 0.0002 0.0006 0.001 0.0033 0.0005 0.003 0.0001 0.0004 0.0001 0.0003 0.0001 0.0046 0.0001 0.0017 0.0005 0.0117 0.0001
Vacuole 0.0017 0.0008 0.0009 0.0035 0.0013 0.0013 0.0121 0.0005 0.001 0.0016 0.0021 0.0006 0.0122 0.0005 0.0024 0.0025 0.002 0.0006
Vacuole Periphery 0.0004 0.0001 0.0002 0.0006 0.0005 0.0002 0.0086 0.0001 0.0003 0.0002 0.0005 0.0001 0.0014 0 0.0005 0.0003 0.002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.5258 23.4851 23.5264 26.494 26.3982 21.0056 31.1664 26.8449 26.5616 30.1693
Translational Efficiency 1.5046 1.5531 1.5541 1.1406 1.5722 1.7855 1.1965 1.0025 1.1293 1.4125

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2062 848 1709 1584 1813 1852 130 1065 3875 2700 1839 2649

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 786.70 886.41 966.46 1070.83 715.44 906.06 1073.00 1000.66 753.36 899.89 973.99 1042.62
Standard Deviation 93.34 122.31 126.51 138.03 95.87 106.56 120.80 145.73 101.00 112.12 129.03 145.31
Intensity Change Log 2 — 0.172161 0.296896 0.444844 — 0.340776 0.584747 0.484049 — 0.254930 0.441168 0.463650

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000127 0.000721 0.000157 0.003027 0.000118 0.000233 0.000279 0.001448 0.000123 0.000386 0.000166 0.002392
Bud Neck 0.012058 0.009209 0.001744 0.006467 0.005788 0.011637 0.001694 0.004567 0.009125 0.010874 0.001740 0.005703
Bud Site 0.009758 0.048582 0.011390 0.055444 0.007195 0.020175 0.008729 0.030998 0.008559 0.029097 0.011201 0.045616
Cell Periphery 0.000105 0.000122 0.000065 0.000308 0.000085 0.000109 0.000159 0.000098 0.000096 0.000113 0.000072 0.000224
Cytoplasm 0.556605 0.628766 0.772564 0.645102 0.623990 0.644274 0.449213 0.584691 0.588133 0.639403 0.749706 0.620815
Cytoplasmic Foci 0.225248 0.189960 0.025086 0.041622 0.196658 0.175460 0.044372 0.050413 0.211872 0.180014 0.026449 0.045156
Eisosomes 0.000124 0.000051 0.000016 0.000024 0.000066 0.000079 0.000075 0.000032 0.000097 0.000070 0.000020 0.000027
Endoplasmic Reticulum 0.001542 0.000473 0.000765 0.000674 0.002292 0.000891 0.002126 0.001470 0.001893 0.000760 0.000861 0.000994
Endosome 0.018746 0.018464 0.003067 0.011159 0.013156 0.015746 0.011420 0.015078 0.016131 0.016599 0.003658 0.012735
Golgi 0.009612 0.005824 0.000276 0.012288 0.003150 0.005455 0.000187 0.006541 0.006589 0.005571 0.000270 0.009977
Lipid Particles 0.004822 0.001085 0.000783 0.000959 0.004667 0.002128 0.002331 0.001287 0.004749 0.001801 0.000893 0.001091
Mitochondria 0.003979 0.000749 0.000167 0.006364 0.003309 0.003987 0.000088 0.007182 0.003666 0.002970 0.000161 0.006693
Mitotic Spindle 0.001729 0.003985 0.007443 0.066656 0.001108 0.004868 0.002459 0.025667 0.001439 0.004591 0.007091 0.050177
None 0.002269 0.000405 0.002967 0.004792 0.001898 0.001209 0.000819 0.006491 0.002095 0.000957 0.002815 0.005475
Nuclear Periphery 0.000125 0.000040 0.000465 0.000453 0.000262 0.000079 0.000762 0.000441 0.000189 0.000066 0.000486 0.000448
Nuclear Periphery Foci 0.000204 0.000595 0.002390 0.001594 0.000505 0.000330 0.010284 0.002339 0.000345 0.000413 0.002948 0.001894
Nucleolus 0.000455 0.000187 0.000175 0.000303 0.000418 0.000297 0.000178 0.001387 0.000437 0.000262 0.000175 0.000739
Nucleus 0.039943 0.010428 0.083389 0.036736 0.047246 0.023793 0.054043 0.031909 0.043360 0.019595 0.081315 0.034795
Peroxisomes 0.002148 0.004388 0.000377 0.003764 0.001399 0.003152 0.000337 0.002632 0.001798 0.003540 0.000374 0.003309
Vacuole 0.106174 0.074339 0.086468 0.094166 0.084877 0.084644 0.410208 0.218335 0.096210 0.081407 0.109354 0.144087
Vacuole Periphery 0.004225 0.001627 0.000246 0.008098 0.001813 0.001454 0.000236 0.006995 0.003097 0.001508 0.000245 0.007654

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.64 -2.56 -3.97 -1.32 -3.72 -3.72 -5.73 -2.09 -1.81 -1.58 -4.73 -3.84 -4.20 -2.94 -3.85
Bud Neck 2.66 14.65 8.23 3.46 -5.56 -5.78 8.80 2.65 7.12 -4.05 -2.07 15.54 8.05 7.53 -6.37
Bud Site -10.39 0.42 -7.35 5.22 -6.68 -8.32 -1.62 -6.67 -1.29 -5.63 -12.89 -0.45 -9.99 1.73 -7.85
Cell Periphery -1.24 6.90 -3.25 -2.25 -5.31 -4.62 -4.96 1.42 4.57 5.29 -3.10 5.80 -2.96 -1.56 -4.93
Cytoplasm -6.55 -18.84 -1.25 4.83 14.24 -2.49 9.70 7.33 9.15 -5.95 -7.97 -13.56 4.14 10.35 15.02
Cytoplasmic Foci 4.85 47.24 41.49 22.86 -5.84 3.71 30.52 31.42 28.01 -2.76 7.26 60.00 51.96 37.71 -8.43
Eisosomes 2.79 3.98 3.93 7.05 -1.11 -3.07 -0.47 10.23 14.09 7.26 1.89 5.24 5.17 17.42 -0.79
Endoplasmic Reticulum 4.97 2.59 5.20 0.36 7.70 3.00 0.29 3.12 0.03 3.74 4.06 3.78 5.47 0.66 3.90
Endosome 0.44 20.33 15.04 9.42 -3.57 -2.45 4.33 3.69 5.27 -0.32 -0.36 23.84 13.41 11.48 -5.59
Golgi 4.60 18.35 6.77 2.33 -3.74 -3.79 13.69 0.49 2.66 -2.98 2.03 21.41 5.45 3.39 -4.75
Lipid Particles 10.69 11.88 9.78 -2.69 -4.77 6.09 6.23 8.30 2.52 2.16 10.36 14.90 12.82 1.57 -4.07
Mitochondria 8.40 11.00 3.45 -1.98 -3.16 -0.54 4.02 0.30 0.73 -2.47 0.92 8.45 2.05 0.82 -3.79
Mitotic Spindle -1.32 -3.44 -13.38 -12.43 -12.13 -3.08 -1.94 -7.95 -6.82 -5.30 -3.14 -4.21 -15.53 -14.15 -13.36
None 3.99 -0.84 -2.81 -9.81 -2.92 2.05 0.96 -1.97 -2.59 -2.29 3.65 -1.49 -3.36 -5.89 -2.61
Nuclear Periphery 4.09 -15.52 -11.69 -13.17 -4.47 4.72 -8.06 -3.93 -7.63 4.63 5.53 -14.67 -11.73 -14.82 -2.35
Nuclear Periphery Foci -4.88 -15.08 -7.25 -5.81 0.04 3.54 -9.09 -4.94 -5.28 5.55 -1.95 -16.27 -8.54 -8.21 0.91
Nucleolus 8.17 9.28 -3.04 -6.52 -6.75 2.10 6.04 -2.43 -2.87 -3.37 4.62 8.81 -3.38 -4.77 -5.59
Nucleus 20.92 -19.60 -4.33 -18.50 14.44 14.11 -2.85 4.05 -7.22 3.95 21.10 -19.13 -1.04 -16.61 17.36
Peroxisomes -5.84 12.42 0.14 4.60 -5.22 -5.18 8.82 -2.40 1.75 -5.60 -6.65 13.34 -1.48 3.86 -7.33
Vacuole 5.91 -6.09 -17.15 -19.89 -10.94 0.09 -14.86 -22.42 -22.57 6.72 4.69 -12.13 -27.79 -29.96 -12.06
Vacuole Periphery 7.15 12.82 3.08 -0.78 -3.06 1.68 9.18 -1.18 -1.63 -3.18 7.49 15.38 1.37 -1.75 -4.40
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Trm9

Trm9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available