Standard name | |
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Human Ortholog | |
Description | tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
Bud | 0 | 3 | 0 | 1 | 2 | 2 | 1 | 2 | 8 | 7 | 13 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 9 | 6 | 8 | 4 | 7 | 7 | 3 | 8 | 4 | 7 | 2 | 0 | 1 | 3 | 11 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 95 | 162 | 285 | 230 | 413 | 364 | 403 | 436 | 351 | 346 | 336 | 336 | 157 | 221 | 257 | 129 | 131 | 155 | 104 | 169 | 268 | 143 | 228 | 218 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 9 | 8 | 10 | 0 | 0 | 2 | 1 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 1 | 6 | 3 | 3 | 3 | 0 | 2 | 0 | 2 | 2 | 3 | 0 | 0 | 2 | 0 | 0 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 2 | 10 | 1 | 10 | 5 | 50 | 78 | 56 | 169 | 114 | 169 | 142 | 0 | 0 | 0 | 5 | 2 | 3 | 2 | 2 | 6 | 2 | 1 | 2 |
Nucleus | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 1 | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 13 | 2 | 1 | 3 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 5 | 3 | 11 | 10 | 4 | 15 | 13 | 7 | 9 | 16 | 1 | 0 | 0 | 4 | 3 | 5 | 1 | 0 | 2 | 4 | 3 | 0 |
Unique Cell Count | 97 | 167 | 289 | 233 | 427 | 391 | 461 | 503 | 444 | 417 | 448 | 449 | 160 | 225 | 258 | 133 | 139 | 166 | 110 | 178 | 295 | 161 | 244 | 238 |
Labelled Cell Count | 100 | 177 | 300 | 253 | 446 | 435 | 506 | 540 | 556 | 490 | 542 | 516 | 162 | 227 | 260 | 153 | 160 | 182 | 110 | 178 | 295 | 161 | 244 | 238 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 4.5 | 6.9 | 6.3 | 6.7 | 5.4 | 5.5 | 6.1 | 4.6 | 4.7 | 4.8 | 4.9 | 6.5 | 6.4 | 6.4 | 6.4 | 7.9 | 8.3 | 7.8 | 7.7 | 7.7 |
Std Deviation (1e-4) | 0.7 | 0.6 | 1.6 | 1.3 | 1.6 | 1.3 | 2.5 | 4.6 | 1.8 | 1.3 | 2.0 | 1.9 | 1.6 | 1.5 | 1.5 | 1.4 | 1.5 | 1.6 | 2.5 | 2.2 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.04 | -0.34 | -0.33 | -0.16 | -0.58 | -0.55 | -0.52 | -0.5 | -0.09 | -0.1 | -0.09 | -0.1 | 0.2 | 0.27 | 0.18 | 0.16 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.6008 | 5.8549 | 5.1885 | 4.5196 | 5.2643 | 5.2659 | 4.5311 | 5.3686 | 5.2454 | 4.2101 | 4.6515 | 5.2815 | 1.8258 | 3.0407 | 2.6653 | 2.6131 | 2.2738 | 2.4976 |
Actin | 0.0228 | 0.0016 | 0.0089 | 0.0684 | 0.0064 | 0.0161 | 0.058 | 0.0002 | 0.0254 | 0.0005 | 0.001 | 0.0195 | 0.0353 | 0.0002 | 0.0219 | 0.0014 | 0.0047 | 0.0001 |
Bud | 0.0006 | 0.0011 | 0.0002 | 0.0004 | 0.0003 | 0.0016 | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0016 | 0.0001 | 0.0007 | 0.0003 | 0.0004 | 0 |
Bud Neck | 0.0008 | 0.0002 | 0.001 | 0.0132 | 0.0005 | 0.0011 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0061 | 0.0001 | 0.0005 | 0.0006 | 0.0008 | 0.0006 |
Bud Periphery | 0.0008 | 0.0012 | 0.0002 | 0.0003 | 0.0008 | 0.0053 | 0.0018 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0017 | 0 | 0.0006 | 0.0002 | 0.0007 | 0 |
Bud Site | 0.0026 | 0.009 | 0.0015 | 0.0021 | 0.0013 | 0.0004 | 0.0031 | 0.0003 | 0.0014 | 0.0002 | 0.001 | 0.0001 | 0.0032 | 0.0004 | 0.0017 | 0.0048 | 0.0007 | 0 |
Cell Periphery | 0.0004 | 0.0005 | 0.0006 | 0.0002 | 0.0004 | 0.0004 | 0.0015 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.7895 | 0.8437 | 0.884 | 0.7166 | 0.6958 | 0.8532 | 0.5232 | 0.9413 | 0.7822 | 0.958 | 0.8034 | 0.8558 | 0.6858 | 0.9713 | 0.8553 | 0.8919 | 0.8111 | 0.9638 |
Cytoplasmic Foci | 0.0219 | 0.007 | 0.0161 | 0.0419 | 0.0256 | 0.0148 | 0.0406 | 0.0029 | 0.0103 | 0.0047 | 0.027 | 0.004 | 0.0681 | 0.002 | 0.0137 | 0.0179 | 0.0303 | 0.0034 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0012 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0105 | 0.0027 | 0.0069 | 0.0034 | 0.0035 | 0.0062 | 0.0068 | 0.0027 | 0.0027 | 0.0034 | 0.0013 | 0.0072 | 0.0123 | 0.002 | 0.0038 | 0.0039 | 0.0022 | 0.0019 |
Endosome | 0.0283 | 0.0062 | 0.0071 | 0.0231 | 0.0467 | 0.0111 | 0.0442 | 0.0013 | 0.0164 | 0.0032 | 0.0454 | 0.003 | 0.0468 | 0.0008 | 0.0145 | 0.0054 | 0.0418 | 0.0023 |
Golgi | 0.0101 | 0.0007 | 0.006 | 0.0057 | 0.0144 | 0.0022 | 0.0406 | 0.0003 | 0.0143 | 0.0002 | 0.0362 | 0.0024 | 0.0181 | 0.0001 | 0.0135 | 0.0024 | 0.0147 | 0.0001 |
Lipid Particles | 0.0051 | 0.0015 | 0.0014 | 0.0116 | 0.0127 | 0.0005 | 0.0316 | 0.0001 | 0.0035 | 0.0001 | 0.0056 | 0.0011 | 0.0053 | 0.0001 | 0.0074 | 0.0079 | 0.0251 | 0 |
Mitochondria | 0.0144 | 0.0004 | 0.0037 | 0.0275 | 0.0035 | 0.0009 | 0.0487 | 0.0001 | 0.001 | 0.0001 | 0.0062 | 0.0005 | 0.0038 | 0 | 0.0099 | 0.0024 | 0.0028 | 0 |
None | 0.0739 | 0.1043 | 0.0538 | 0.0367 | 0.174 | 0.0824 | 0.1399 | 0.0492 | 0.1335 | 0.026 | 0.0622 | 0.1035 | 0.053 | 0.0215 | 0.0284 | 0.0403 | 0.0084 | 0.0262 |
Nuclear Periphery | 0.0016 | 0.0004 | 0.0007 | 0.0008 | 0.0018 | 0.0004 | 0.005 | 0.0003 | 0.0005 | 0.0005 | 0.0004 | 0.0003 | 0.0106 | 0.0002 | 0.0066 | 0.0004 | 0.0258 | 0.0002 |
Nucleolus | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0022 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0034 | 0 |
Nucleus | 0.0013 | 0.0005 | 0.0005 | 0.001 | 0.0012 | 0.0006 | 0.0036 | 0.0003 | 0.0005 | 0.0006 | 0.0006 | 0.0004 | 0.0124 | 0.0004 | 0.0049 | 0.0007 | 0.0071 | 0.0004 |
Peroxisomes | 0.0037 | 0.0177 | 0.0056 | 0.0417 | 0.0049 | 0.0006 | 0.0229 | 0 | 0.0056 | 0 | 0.0058 | 0.0008 | 0.0165 | 0 | 0.0114 | 0.0158 | 0.0042 | 0 |
Punctate Nuclear | 0.0086 | 0.0002 | 0.0006 | 0.001 | 0.0033 | 0.0005 | 0.003 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0046 | 0.0001 | 0.0017 | 0.0005 | 0.0117 | 0.0001 |
Vacuole | 0.0017 | 0.0008 | 0.0009 | 0.0035 | 0.0013 | 0.0013 | 0.0121 | 0.0005 | 0.001 | 0.0016 | 0.0021 | 0.0006 | 0.0122 | 0.0005 | 0.0024 | 0.0025 | 0.002 | 0.0006 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0006 | 0.0005 | 0.0002 | 0.0086 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0014 | 0 | 0.0005 | 0.0003 | 0.002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.5258 | 23.4851 | 23.5264 | 26.494 | 26.3982 | 21.0056 | 31.1664 | 26.8449 | 26.5616 | 30.1693 |
Translational Efficiency | 1.5046 | 1.5531 | 1.5541 | 1.1406 | 1.5722 | 1.7855 | 1.1965 | 1.0025 | 1.1293 | 1.4125 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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