Standard name
Human Ortholog
Description Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 1.0 0.94 0.95 0.96 0.88 0.81 – 0.77 0.7 0.71 0.64 0.99 0.98 0.97 0.98 0.97 0.98 0.94 0.96 0.91 0.95 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0 – 0.13 0.15 0.22 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.08 0.16 – 0.13 0.12 0.08 0.12 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 1 0 2 2 1
Bud 0 1 0 0 0 2 0 – 1 3 2 1 0 0 0 0 0 0 3 3 0 2 11 13
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 2 1 1 3 0 5 – 6 11 2 0 1 3 10 3 8 4 0 0 0 0 0 0
Cytoplasm 262 371 87 81 103 119 163 – 144 167 85 54 359 582 658 216 174 200 254 357 98 348 520 510
Endoplasmic Reticulum 1 0 1 0 0 1 5 – 2 8 0 1 1 2 4 11 6 3 0 0 2 3 5 4
Endosome 2 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0
Mitochondria 1 0 0 2 1 16 4 – 24 35 26 23 0 0 0 2 1 2 0 0 0 0 0 1
Nucleus 5 2 4 0 1 3 4 – 4 11 6 2 4 6 6 0 0 0 1 2 2 0 0 3
Nuclear Periphery 0 0 0 1 0 1 0 – 0 0 0 5 1 0 0 0 0 0 2 0 0 1 2 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 5 0 3 2 3 11 32 – 25 30 10 10 3 7 7 2 4 3 1 2 0 4 3 4
Unique Cell Count 271 372 93 85 107 136 201 186 240 119 85 364 594 675 221 180 205 270 373 108 367 556 547
Labelled Cell Count 281 376 96 87 111 153 213 206 266 131 98 369 600 685 234 195 213 270 373 108 367 556 547


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.4 18.0 18.0 14.6 14.8 8.1 10.2 – 8.1 8.1 7.1 6.8 17.5 16.8 16.4 23.8 25.1 22.9 18.0 17.9 18.1
Std Deviation (1e-4) 6.0 4.5 4.5 4.4 3.6 2.6 2.2 – 1.8 1.8 1.8 1.4 4.7 4.1 4.0 5.4 5.8 5.8 4.7 5.1 5.2
Intensity Change (Log2) – – – -0.3 -0.28 -1.15 -0.83 – -1.15 -1.15 -1.35 -1.41 -0.04 -0.11 -0.14 0.4 0.48 0.34 -0.01 -0.01 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0.9 -1.5 -2.8 – -3.4 -4.6 -4.1 -4.9 2.9 2.5 2.1 1.8 1.2 1.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 0 – 3.6 3.9 4.8 5.4 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 1.5 3.1 – 2.7 2.5 1.6 2.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.2203 11.7702 11.0653 10.4769 9.9973 10.8177 13.9799 16.803 16.091 14.2909 16.1118 15.782 15.8295 19.1351 17.8384 14.0176 17.5468 17.274
Actin 0.0031 0.0011 0.0012 0.0005 0.0002 0.0003 0.0015 0.0009 0.0007 0.0013 0.0006 0.001 0.0056 0.0004 0.0005 0.0013 0.0009 0.0009
Bud 0.0003 0.0007 0.0018 0.0002 0.0003 0.0001 0.0019 0.0016 0.0021 0.0006 0.0007 0.0004 0.0003 0.0009 0.0013 0.0004 0.0027 0.0002
Bud Neck 0.0005 0.0003 0.0003 0.0007 0.0006 0.0012 0.0007 0.0004 0.0008 0.001 0.0009 0.0015 0.0004 0.0002 0.0003 0.0005 0.0004 0.0012
Bud Periphery 0.0002 0.0004 0.0009 0 0.0001 0 0.0011 0.0006 0.0009 0.0003 0.0005 0.0004 0.0001 0.001 0.0009 0.0001 0.0019 0.0001
Bud Site 0.0004 0.0043 0.0031 0.0001 0 0 0.0014 0.0019 0.004 0.0015 0.0002 0.0001 0.0005 0.0002 0.0002 0.0003 0.0003 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0
Cytoplasm 0.9344 0.9769 0.972 0.9731 0.9819 0.9684 0.9496 0.9575 0.9373 0.9347 0.9639 0.9528 0.9436 0.9858 0.9795 0.9682 0.9798 0.9656
Cytoplasmic Foci 0.0095 0.0024 0.0016 0.0023 0.0023 0.0024 0.002 0.0017 0.002 0.0031 0.0016 0.0026 0.0097 0.0009 0.0011 0.0025 0.0011 0.0042
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0032 0.0007 0.0007 0.0005 0.0008 0.002 0.003 0.0026 0.0019 0.0023 0.0018 0.0017 0.0028 0.0006 0.0007 0.0046 0.0013 0.0017
Endosome 0.0098 0.0004 0.0004 0.0008 0.0006 0.0015 0.001 0.0006 0.001 0.0006 0.0009 0.0011 0.0021 0.0003 0.0003 0.0012 0.0004 0.0017
Golgi 0.0029 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0015 0 0 0.0001 0 0.0001
Lipid Particles 0.0033 0 0 0 0 0 0 0 0 0 0 0 0.0034 0 0 0 0 0.0001
Mitochondria 0.0071 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
None 0.008 0.003 0.0024 0.0018 0.0015 0.0014 0.0058 0.0025 0.0047 0.0048 0.0025 0.0022 0.0099 0.0013 0.002 0.0085 0.0017 0.0044
Nuclear Periphery 0.0026 0.0011 0.0014 0.0013 0.0014 0.0024 0.0036 0.0029 0.0033 0.0042 0.0025 0.0024 0.002 0.0007 0.001 0.0024 0.0017 0.0016
Nucleolus 0.0001 0 0 0 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0 0 0
Nucleus 0.0098 0.007 0.0128 0.0173 0.009 0.0185 0.0248 0.0243 0.0369 0.0411 0.02 0.031 0.0105 0.0067 0.0111 0.0077 0.0067 0.0148
Peroxisomes 0.0014 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0004 0 0.0001 0.0053 0 0 0 0 0.0001
Punctate Nuclear 0.0015 0.0008 0.0007 0.0008 0.0003 0.0006 0.0008 0.001 0.0015 0.0025 0.0019 0.0015 0.0011 0.0003 0.0003 0.0007 0.0003 0.0017
Vacuole 0.0013 0.0004 0.0003 0.0003 0.0005 0.0007 0.0018 0.0011 0.0017 0.0012 0.0017 0.0008 0.0007 0.0005 0.0004 0.001 0.0005 0.0013
Vacuole Periphery 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 259.2218 279.823 266.0397 208.896 195.3141 238.5476 209.9862 173.0556 194.996 189.717
Translational Efficiency 1.3675 1.3028 1.3952 1.6614 1.8059 1.4649 1.564 1.9964 1.5511 1.7433

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1704 541 195 261 1358 2052 141 1158 3062 2593 336 1419

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1297.98 1550.75 2262.80 2173.00 1274.89 1561.03 2370.85 2603.81 1287.74 1558.89 2308.14 2524.57
Standard Deviation 276.59 382.39 407.95 686.51 237.65 337.29 466.24 710.05 260.29 347.21 436.63 725.25
Intensity Change Log 2 — 0.256698 0.801841 0.743420 — 0.292125 0.895032 1.030251 — 0.274362 0.848771 0.892669

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000140 0.000219 0.000650 0.001439 0.000676 0.000209 0.001009 0.000678 0.000378 0.000211 0.000801 0.000818
Bud Neck 0.000403 0.000496 0.002803 0.004154 0.000367 0.000565 0.003645 0.002658 0.000387 0.000551 0.003156 0.002933
Bud Site 0.003254 0.003637 0.004880 0.008468 0.004759 0.003262 0.005464 0.016481 0.003922 0.003340 0.005125 0.015007
Cell Periphery 0.000067 0.000133 0.000828 0.000367 0.000106 0.000046 0.000712 0.000217 0.000085 0.000064 0.000780 0.000245
Cytoplasm 0.890310* 0.861592* 0.601056* 0.540794* 0.874902* 0.875455* 0.586671* 0.733117* 0.883477* 0.872562* 0.595020* 0.697743*
Cytoplasmic Foci 0.019999 0.014140 0.028188 0.021336 0.024660 0.012227 0.021497 0.013000 0.022066 0.012626 0.025380 0.014534
Eisosomes 0.000003 0.000003 0.000005 0.000047 0.000005 0.000002 0.000008 0.000004 0.000003 0.000002 0.000006 0.000012
Endoplasmic Reticulum 0.000316 0.000283 0.000209 0.001290 0.000862 0.000174 0.000606 0.000152 0.000558 0.000197 0.000376 0.000362
Endosome 0.000393 0.001011 0.001904 0.017346 0.000934 0.000750 0.000919 0.002888 0.000633 0.000804 0.001491 0.005547
Golgi 0.000624 0.002010 0.005194 0.003024 0.001129 0.001341 0.000757 0.009933 0.000848 0.001480 0.003332 0.008662
Lipid Particles 0.000243 0.000307 0.001658 0.022027 0.000535 0.000256 0.002024 0.000915 0.000372 0.000266 0.001812 0.004798
Mitochondria 0.000494 0.000079 0.000199 0.001503 0.000314 0.000269 0.000126 0.002199 0.000414 0.000229 0.000168 0.002071
Mitotic Spindle 0.002059 0.000993 0.003523 0.007179 0.003470 0.006032 0.002548 0.014716 0.002685 0.004981 0.003114 0.013330
None 0.009487 0.014998 0.006851 0.026288 0.010902 0.017588 0.005085 0.014371 0.010115 0.017047 0.006110 0.016563
Nuclear Periphery 0.000057 0.000049 0.000387 0.000374 0.000112 0.000043 0.000363 0.000127 0.000082 0.000044 0.000377 0.000172
Nuclear Periphery Foci 0.002377 0.003487 0.003682 0.004776 0.005651 0.001200 0.006706 0.001514 0.003829 0.001677 0.004951 0.002114
Nucleolus 0.000117 0.001887 0.000108 0.009894 0.000097 0.000154 0.000129 0.000128 0.000108 0.000515 0.000117 0.001924
Nucleus 0.059787 0.084641 0.314983* 0.232386* 0.060039 0.074975 0.316460* 0.175341* 0.059898 0.076992 0.315603* 0.185833*
Peroxisomes 0.000078 0.000114 0.000088 0.007859 0.000096 0.000454 0.000081 0.000378 0.000086 0.000383 0.000085 0.001754
Vacuole 0.008780 0.009187 0.022671 0.088523 0.009660 0.004711 0.045056 0.009756 0.009170 0.005645 0.032065 0.024244
Vacuole Periphery 0.001013 0.000736 0.000132 0.000925 0.000723 0.000288 0.000134 0.001425 0.000885 0.000381 0.000133 0.001333

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.01 -6.03 -4.53 -3.73 2.06 1.70 -1.75 1.02 -1.86 4.33 1.27 -4.69 -0.51 -2.88 5.22
Bud Neck -2.04 -8.69 -5.67 -5.49 -0.10 -2.63 -7.11 -7.06 -5.97 5.33 -2.88 -11.06 -8.86 -8.03 6.18
Bud Site -0.67 -4.50 -4.95 -4.11 -1.17 1.32 -3.34 -2.07 -3.41 1.06 0.78 -5.37 -3.69 -4.20 0.25
Cell Periphery -5.09 -9.45 -6.87 -5.78 5.31 5.48 -8.01 -7.64 -11.56 6.83 3.13 -12.39 -11.32 -12.46 9.79
Cytoplasm 4.87 21.44 25.78 22.77 2.50 0.88 19.74 24.75 25.19 -9.81 4.15 29.06 33.91 31.04 -9.86
Cytoplasmic Foci 2.87 -4.69 0.74 -1.16 4.64 7.34 -2.55 6.09 -1.16 4.78 7.57 -5.26 5.42 -1.52 7.32
Eisosomes -0.03 -6.75 -4.62 -4.58 -1.94 4.33 -5.77 0.96 -4.67 6.20 3.53 -8.82 -4.41 -5.93 4.83
Endoplasmic Reticulum 0.54 -1.36 -2.68 -2.79 -1.60 4.42 1.88 5.48 1.43 3.05 4.24 1.05 5.20 0.25 3.79
Endosome -1.10 -1.89 -1.90 -1.55 -1.26 0.85 -0.27 0.63 -0.20 0.74 -0.45 -1.85 -1.76 -1.29 -0.13
Golgi -1.05 -1.20 -0.87 0.78 1.02 -0.25 0.44 -1.14 -0.97 -1.27 -1.18 -1.12 -1.42 -0.76 -0.13
Lipid Particles -1.49 -7.38 -2.51 -2.47 -0.97 1.85 -5.78 -3.98 -6.32 4.76 1.13 -9.31 -3.82 -4.01 3.26
Mitochondria 2.14 1.18 0.10 -2.41 -1.26 0.36 0.89 -0.81 -0.99 -1.19 1.33 1.44 -0.54 -1.26 -1.25
Mitotic Spindle 1.52 -1.79 -2.83 -3.53 -1.24 -1.33 -0.13 -2.39 -1.03 -1.95 -1.68 -1.42 -3.70 -1.87 -2.10
None -2.93 4.87 0.16 1.99 -1.72 -4.50 4.92 0.75 6.87 -6.20 -6.61 6.89 0.30 6.93 -6.61
Nuclear Periphery 0.14 -10.40 -11.23 -11.40 -0.00 1.64 -7.05 -4.34 -17.09 5.72 1.66 -12.26 -12.09 -18.94 7.34
Nuclear Periphery Foci -3.13 -5.08 -2.64 0.60 3.33 8.76 -1.73 7.01 -1.59 5.61 7.67 -4.45 5.37 -1.03 7.35
Nucleolus -1.06 -1.75 -1.46 -0.39 -1.43 -1.06 -4.36 -1.36 0.58 3.27 -1.16 -3.62 -1.49 -0.22 -1.28
Nucleus -5.82 -17.57 -12.36 -9.72 4.35 -6.89 -14.65 -23.97 -20.37 5.65 -9.16 -22.88 -27.37 -22.44 8.58
Peroxisomes -0.64 -1.31 -1.55 -1.52 -1.52 -2.02 -0.46 -0.85 1.60 -0.71 -2.09 -1.30 -1.75 -0.12 -1.65
Vacuole -1.00 -7.66 -14.04 -13.87 -7.51 3.95 -8.03 -14.38 -15.50 4.36 3.09 -11.04 -18.58 -19.07 1.26
Vacuole Periphery 0.62 2.33 1.91 0.77 -1.33 1.47 2.11 1.17 -0.39 -1.12 1.90 3.07 1.99 -0.01 -1.27
Standard name
Human Ortholog
Description Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Apt1

Apt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available