Standard name | |
---|---|
Human Ortholog | |
Description | Scaffold subunit of the Hrd1p ubiquitin ligase; also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 16 | 3 | 2 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 16 | 2 | 10 | 36 | 19 | 24 | 51 | 47 |
Bud | 6 | 10 | 2 | 1 | 2 | 1 | 4 | 1 | 0 | 0 | 4 | 0 | 2 | 1 | 1 | 0 | 7 | 0 | 1 | 2 | 2 | 1 | 3 | 1 |
Bud Neck | 1 | 1 | 2 | 0 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 2 | 7 | 5 | 4 | 11 | 13 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 7 | 10 | 10 | 11 | 3 | 3 | 4 | 0 | 3 | 0 | 1 | 12 | 5 | 8 | 6 | 6 | 1 | 5 | 13 | 16 | 16 | 40 | 31 |
Cytoplasm | 45 | 142 | 153 | 113 | 137 | 139 | 203 | 216 | 92 | 85 | 49 | 30 | 126 | 199 | 272 | 49 | 57 | 16 | 17 | 57 | 38 | 18 | 20 | 16 |
Endoplasmic Reticulum | 32 | 45 | 2 | 2 | 7 | 8 | 7 | 1 | 3 | 4 | 0 | 0 | 15 | 14 | 4 | 5 | 2 | 3 | 60 | 77 | 39 | 32 | 73 | 55 |
Endosome | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 9 | 2 | 1 | 4 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 4 |
Mitochondria | 12 | 7 | 5 | 16 | 17 | 22 | 44 | 31 | 43 | 50 | 49 | 23 | 3 | 3 | 1 | 8 | 4 | 4 | 17 | 55 | 57 | 29 | 49 | 34 |
Nucleus | 0 | 2 | 0 | 1 | 0 | 1 | 3 | 2 | 3 | 2 | 1 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 6 | 2 | 1 | 2 | 1 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 2 | 3 | 2 | 3 | 7 | 12 | 7 | 4 | 14 | 11 |
Unique Cell Count | 87 | 212 | 167 | 130 | 163 | 166 | 241 | 236 | 120 | 128 | 80 | 46 | 146 | 218 | 283 | 65 | 81 | 26 | 128 | 285 | 199 | 141 | 280 | 229 |
Labelled Cell Count | 103 | 237 | 180 | 146 | 179 | 181 | 272 | 262 | 143 | 150 | 104 | 54 | 161 | 227 | 294 | 76 | 101 | 31 | 128 | 285 | 199 | 141 | 280 | 229 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 4.9 | 5.3 | 5.1 | 5.5 | 4.4 | 4.6 | 4.8 | 4.3 | 4.3 | 3.8 | 3.9 | 5.1 | 5.2 | 5.1 | 6.7 | 6.9 | 6.3 | 4.7 | 4.9 | 5.5 |
Std Deviation (1e-4) | 0.6 | 0.7 | 0.7 | 1.4 | 1.1 | 1.2 | 1.4 | 1.7 | 1.9 | 1.9 | 1.7 | 0.8 | 1.1 | 1.3 | 1.5 | 1.5 | 1.6 | 1.4 | 1.1 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.05 | -0.27 | -0.21 | -0.14 | -0.3 | -0.28 | -0.48 | -0.43 | -0.04 | -0.02 | -0.03 | 0.35 | 0.38 | 0.25 | -0.17 | -0.1 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.6 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | -1.9 | -1.7 | 0 | 0.4 | 0 |
Cytoplasm | -1.3 | -2.1 | -2.2 | -2.2 | 0 | -3.5 | -5.4 | -5.8 | -4.6 | -1.5 | -0.1 | 2.0 | -3.3 | -4.4 | -4.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 2.5 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.1 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7841 | 3.2328 | 2.8649 | 2.8599 | 2.7965 | 3.1198 | 1.575 | 1.5217 | 1.4842 | 1.0327 | 1.7453 | 1.3501 | 0.7209 | 0.2326 | 0.3163 | -0.3114 | 0.0307 | 0.6181 |
Actin | 0.032 | 0.0217 | 0.0158 | 0.0259 | 0.0222 | 0.0325 | 0.0529 | 0.0157 | 0.0532 | 0.0256 | 0.029 | 0.0182 | 0.0624 | 0.015 | 0.0332 | 0.0038 | 0.0813 | 0.0039 |
Bud | 0.0005 | 0.0004 | 0.0009 | 0.0009 | 0.0003 | 0.001 | 0.0017 | 0.0026 | 0.0015 | 0.0019 | 0.0019 | 0.0011 | 0.0013 | 0.0002 | 0.0011 | 0.0002 | 0.0004 | 0.0004 |
Bud Neck | 0.0027 | 0.0014 | 0.0022 | 0.0212 | 0.0031 | 0.0458 | 0.0114 | 0.0032 | 0.0131 | 0.0038 | 0.0232 | 0.026 | 0.0046 | 0.0009 | 0.0044 | 0.0012 | 0.0022 | 0.0317 |
Bud Periphery | 0.001 | 0.001 | 0.0037 | 0.0031 | 0.001 | 0.0033 | 0.0055 | 0.0061 | 0.0036 | 0.0072 | 0.0048 | 0.0065 | 0.0019 | 0.0005 | 0.0029 | 0.0004 | 0.001 | 0.0008 |
Bud Site | 0.0093 | 0.0333 | 0.01 | 0.0021 | 0.0008 | 0.0024 | 0.0156 | 0.0899 | 0.0304 | 0.0017 | 0.0181 | 0.0023 | 0.0347 | 0.0266 | 0.0105 | 0.001 | 0.0012 | 0.0115 |
Cell Periphery | 0.011 | 0.0203 | 0.0135 | 0.0065 | 0.0092 | 0.009 | 0.0093 | 0.0155 | 0.008 | 0.0271 | 0.0046 | 0.0047 | 0.0106 | 0.0079 | 0.0052 | 0.0016 | 0.0019 | 0.0024 |
Cytoplasm | 0.3538 | 0.4394 | 0.4291 | 0.3248 | 0.4427 | 0.4679 | 0.2971 | 0.4553 | 0.3616 | 0.3469 | 0.3014 | 0.4694 | 0.225 | 0.3435 | 0.4408 | 0.4061 | 0.4602 | 0.4642 |
Cytoplasmic Foci | 0.0439 | 0.0123 | 0.0281 | 0.0417 | 0.0306 | 0.034 | 0.0425 | 0.0192 | 0.045 | 0.0164 | 0.072 | 0.0405 | 0.0709 | 0.0335 | 0.0251 | 0.0478 | 0.0953 | 0.0134 |
Eisosomes | 0.0019 | 0.0013 | 0.0024 | 0.0015 | 0.0009 | 0.0014 | 0.0027 | 0.0019 | 0.002 | 0.0011 | 0.0015 | 0.0008 | 0.001 | 0.0008 | 0.0008 | 0.0005 | 0.0013 | 0.0002 |
Endoplasmic Reticulum | 0.1236 | 0.0915 | 0.1068 | 0.1451 | 0.1303 | 0.087 | 0.1621 | 0.1069 | 0.157 | 0.2287 | 0.0433 | 0.1536 | 0.0933 | 0.1158 | 0.0907 | 0.0667 | 0.1491 | 0.0998 |
Endosome | 0.029 | 0.0083 | 0.0285 | 0.0128 | 0.0244 | 0.016 | 0.0605 | 0.016 | 0.0415 | 0.0252 | 0.0985 | 0.0271 | 0.0369 | 0.0276 | 0.0231 | 0.0374 | 0.0103 | 0.0139 |
Golgi | 0.0112 | 0.0035 | 0.0108 | 0.0096 | 0.0048 | 0.0064 | 0.0236 | 0.0053 | 0.0168 | 0.0095 | 0.024 | 0.0107 | 0.0085 | 0.0067 | 0.0087 | 0.007 | 0.0122 | 0.0035 |
Lipid Particles | 0.0367 | 0.0087 | 0.0167 | 0.0091 | 0.0048 | 0.0081 | 0.0582 | 0.0134 | 0.0224 | 0.0178 | 0.0398 | 0.0129 | 0.0257 | 0.0169 | 0.0153 | 0.0163 | 0.024 | 0.0098 |
Mitochondria | 0.0062 | 0.0007 | 0.0105 | 0.0067 | 0.0028 | 0.0043 | 0.0191 | 0.0031 | 0.0114 | 0.004 | 0.0488 | 0.0124 | 0.0071 | 0.0008 | 0.0145 | 0.0018 | 0.0095 | 0.0024 |
None | 0.3034 | 0.3426 | 0.2964 | 0.3465 | 0.2905 | 0.2559 | 0.1714 | 0.2183 | 0.1976 | 0.2527 | 0.1452 | 0.1797 | 0.319 | 0.3934 | 0.307 | 0.3981 | 0.1296 | 0.3183 |
Nuclear Periphery | 0.0087 | 0.0045 | 0.007 | 0.0044 | 0.0121 | 0.0023 | 0.0238 | 0.0033 | 0.0078 | 0.0149 | 0.0195 | 0.0061 | 0.0072 | 0.0025 | 0.0029 | 0.002 | 0.0039 | 0.0027 |
Nucleolus | 0.001 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0004 | 0.0015 | 0.0026 | 0.0017 | 0.0007 | 0.0018 | 0.0014 | 0.0051 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0015 |
Nucleus | 0.0034 | 0.0022 | 0.0024 | 0.0019 | 0.0081 | 0.003 | 0.005 | 0.0063 | 0.0045 | 0.0034 | 0.0039 | 0.0069 | 0.0036 | 0.001 | 0.0015 | 0.0011 | 0.0009 | 0.0022 |
Peroxisomes | 0.0091 | 0.0004 | 0.0058 | 0.0305 | 0.0032 | 0.0133 | 0.014 | 0.0015 | 0.0057 | 0.0007 | 0.0738 | 0.0037 | 0.0321 | 0.0005 | 0.005 | 0.0009 | 0.0107 | 0.0118 |
Punctate Nuclear | 0.0072 | 0.0007 | 0.0016 | 0.0008 | 0.0051 | 0.002 | 0.0093 | 0.0078 | 0.0046 | 0.0014 | 0.0037 | 0.0088 | 0.035 | 0.0012 | 0.001 | 0.0014 | 0.0026 | 0.0027 |
Vacuole | 0.0037 | 0.0052 | 0.0065 | 0.0041 | 0.0025 | 0.0035 | 0.0101 | 0.0058 | 0.0091 | 0.0077 | 0.0318 | 0.0059 | 0.0123 | 0.0042 | 0.0051 | 0.0039 | 0.0017 | 0.0028 |
Vacuole Periphery | 0.0006 | 0.0003 | 0.001 | 0.0006 | 0.0005 | 0.0005 | 0.0029 | 0.0004 | 0.0017 | 0.0016 | 0.0093 | 0.0013 | 0.0017 | 0.0003 | 0.0009 | 0.0005 | 0.0006 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.4252 | 12.1485 | 11.9226 | 14.9071 | 12.2202 | 13.5823 | 14.1654 | 16.0459 | 15.7047 | 15.6387 |
Translational Efficiency | 1.1119 | 0.6244 | 0.8331 | 0.7795 | 0.7616 | 0.8604 | 0.6399 | 0.7161 | 0.8289 | 0.778 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1490 | 794 | 1293 | 1341 | 1700 | 1421 | 1160 | 533 | 3190 | 2215 | 2453 | 1874 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 643.06 | 768.31 | 1012.50 | 975.33 | 706.06 | 626.55 | 959.27 | 1253.35 | 676.63 | 677.37 | 987.33 | 1054.40 |
Standard Deviation | 74.02 | 115.25 | 129.11 | 184.59 | 83.30 | 102.39 | 113.90 | 186.34 | 85.12 | 126.92 | 125.01 | 223.58 |
Intensity Change Log 2 | — | 0.256735 | 0.654897 | 0.600937 | — | -0.172361 | 0.442146 | 0.827927 | — | 0.048102 | 0.547473 | 0.724171 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004030 | 0.006050 | 0.024740 | 0.142812* | 0.002144 | 0.003020 | 0.051506 | 0.103805* | 0.003025 | 0.004106 | 0.037397 | 0.131717* |
Bud Neck | 0.041583 | 0.021654 | 0.014696 | 0.024541 | 0.018059 | 0.031213 | 0.017543 | 0.060750 | 0.029047 | 0.027786 | 0.016043 | 0.034839 |
Bud Site | 0.013287 | 0.014989 | 0.044559 | 0.106196* | 0.008997 | 0.039477 | 0.043116 | 0.069687 | 0.011001 | 0.030699 | 0.043877 | 0.095812 |
Cell Periphery | 0.002694 | 0.002195 | 0.008310 | 0.020510 | 0.001817 | 0.004688 | 0.026388 | 0.030790 | 0.002226 | 0.003795 | 0.016859 | 0.023434 |
Cytoplasm | 0.345683* | 0.273137* | 0.173467* | 0.156875* | 0.398818* | 0.350487* | 0.192291* | 0.260979* | 0.374000* | 0.322760* | 0.182369* | 0.186484* |
Cytoplasmic Foci | 0.231810* | 0.250543* | 0.032035 | 0.046425 | 0.263390* | 0.215279* | 0.023771 | 0.017501 | 0.248640* | 0.227920* | 0.028127 | 0.038199 |
Eisosomes | 0.010662 | 0.005100 | 0.004062 | 0.019417 | 0.005107 | 0.004031 | 0.026805 | 0.098847 | 0.007701 | 0.004414 | 0.014817 | 0.042009 |
Endoplasmic Reticulum | 0.018981 | 0.072529 | 0.472122* | 0.243892* | 0.023757 | 0.022270 | 0.444632* | 0.086444 | 0.021526 | 0.040286 | 0.459122* | 0.199111* |
Endosome | 0.026551 | 0.031361 | 0.011756 | 0.015641 | 0.014058 | 0.018913 | 0.006118 | 0.006167 | 0.019893 | 0.023375 | 0.009090 | 0.012946 |
Golgi | 0.048245 | 0.058152 | 0.008188 | 0.024587 | 0.037680 | 0.032031 | 0.016615 | 0.012037 | 0.042615 | 0.041395 | 0.012173 | 0.021017 |
Lipid Particles | 0.090812 | 0.094482 | 0.045479 | 0.085573 | 0.072003 | 0.071542 | 0.056357 | 0.067534 | 0.080788 | 0.079765 | 0.050623 | 0.080442 |
Mitochondria | 0.028469 | 0.027360 | 0.001073 | 0.004842 | 0.012540 | 0.057311 | 0.001671 | 0.027928 | 0.019980 | 0.046575 | 0.001356 | 0.011408 |
Mitotic Spindle | 0.000707 | 0.004548 | 0.002959 | 0.005917 | 0.000678 | 0.002227 | 0.000370 | 0.000339 | 0.000692 | 0.003059 | 0.001735 | 0.004330 |
None | 0.004682 | 0.000899 | 0.001428 | 0.001146 | 0.002996 | 0.011734 | 0.001388 | 0.002806 | 0.003784 | 0.007850 | 0.001409 | 0.001618 |
Nuclear Periphery | 0.000352 | 0.000908 | 0.002446 | 0.000289 | 0.000561 | 0.000711 | 0.000518 | 0.000218 | 0.000464 | 0.000781 | 0.001534 | 0.000269 |
Nuclear Periphery Foci | 0.000654 | 0.002568 | 0.033345 | 0.006469 | 0.001350 | 0.002334 | 0.011729 | 0.000681 | 0.001025 | 0.002418 | 0.023123 | 0.004823 |
Nucleolus | 0.000813 | 0.000474 | 0.000368 | 0.000114 | 0.000681 | 0.001838 | 0.000095 | 0.000357 | 0.000743 | 0.001349 | 0.000239 | 0.000183 |
Nucleus | 0.065413 | 0.055036 | 0.069316 | 0.022997 | 0.086911 | 0.073520 | 0.038986 | 0.057555 | 0.076870 | 0.066894 | 0.054973 | 0.032826 |
Peroxisomes | 0.027257 | 0.030923 | 0.003059 | 0.019707 | 0.017031 | 0.032455 | 0.010567 | 0.075533 | 0.021807 | 0.031906 | 0.006610 | 0.035585 |
Vacuole | 0.034644 | 0.043647 | 0.046191 | 0.049435 | 0.029871 | 0.019875 | 0.029278 | 0.019422 | 0.032100 | 0.028396 | 0.038193 | 0.040899 |
Vacuole Periphery | 0.002672 | 0.003446 | 0.000403 | 0.002614 | 0.001550 | 0.005044 | 0.000254 | 0.000619 | 0.002074 | 0.004471 | 0.000333 | 0.002047 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.37 | -9.66 | -23.52 | -23.06* | -18.82 | -2.40 | -13.82 | -14.62 | -14.50 | -6.69 | -2.71 | -16.78 | -27.58 | -27.32* | -18.40 |
Bud Neck | 6.58 | 10.58 | 6.26 | -1.14 | -5.10 | -5.39 | 0.24 | -8.26 | -5.44 | -8.32 | 0.66 | 8.34 | -2.55 | -2.95 | -9.19 |
Bud Site | -0.91 | -9.66 | -19.35 | -18.62 | -10.82 | -9.55 | -10.85 | -10.54 | -4.72 | -4.08 | -9.10 | -14.54 | -22.31 | -15.33 | -11.88 |
Cell Periphery | 0.82 | -6.02 | -10.31 | -11.16 | -6.80 | -6.91 | -12.90 | -11.46 | -10.21 | -1.36 | -4.11 | -14.16 | -15.20 | -14.08 | -3.71 |
Cytoplasm | 7.62 | 20.94 | 24.11 | 12.75 | 2.01 | 5.86 | 26.81 | 14.01 | 8.57 | -6.95 | 8.10 | 33.92 | 31.30 | 20.44 | -0.87 |
Cytoplasmic Foci | -2.20 | 40.15 | 35.27 | 29.17 | -5.04 | 7.51 | 51.54 | 51.71 | 39.64 | 2.70 | 4.25 | 64.56 | 56.92 | 44.79 | -4.85 |
Eisosomes | 6.07 | 7.52 | -5.86 | -11.01 | -12.11 | 2.02 | -11.30 | -15.69 | -15.91 | -11.54 | 6.51 | -6.78 | -16.09 | -17.95 | -11.87 |
Endoplasmic Reticulum | -9.49 | -45.98* | -25.70 | -16.63 | 17.68 | 0.58 | -41.47* | -8.41 | -8.53 | 28.88* | -7.13 | -61.83 | -26.13 | -22.27 | 27.21 |
Endosome | -1.78 | 9.65 | 6.27 | 6.45 | -2.82 | -3.85 | 9.95 | 5.82 | 7.98 | -0.09 | -2.63 | 12.73 | 6.20 | 7.71 | -3.87 |
Golgi | -2.68 | 19.40 | 9.52 | 10.08 | -9.11 | 2.45 | 11.58 | 12.55 | 9.64 | 3.01 | 0.69 | 22.32 | 12.57 | 10.66 | -6.32 |
Lipid Particles | -0.61 | 10.71 | 0.99 | 1.47 | -8.78 | 0.09 | 3.83 | 0.76 | 0.68 | -1.67 | 0.31 | 10.43 | 0.11 | -0.15 | -7.89 |
Mitochondria | 0.40 | 15.17 | 12.47 | 8.17 | -5.79 | -13.02 | 11.45 | -5.64 | 7.08 | -10.27 | -10.40 | 18.76 | 6.60 | 14.05 | -11.20 |
Mitotic Spindle | -2.33 | -3.10 | -4.28 | -0.45 | -2.17 | -2.35 | 1.51 | 1.43 | 3.21 | 0.14 | -3.19 | -2.52 | -4.12 | -1.10 | -2.80 |
None | 4.30 | 3.21 | 3.67 | -0.41 | 0.36 | -4.84 | 2.32 | 0.39 | 4.92 | -1.91 | -3.32 | 3.97 | 3.87 | 5.23 | -0.38 |
Nuclear Periphery | -2.30 | -10.69 | 0.69 | 2.45 | 10.90 | -1.73 | 0.49 | 3.97 | 4.89 | 3.38 | -3.11 | -9.72 | 3.78 | 4.76 | 11.21 |
Nuclear Periphery Foci | -5.96 | -15.08 | -9.71 | -5.95 | 11.76 | -1.56 | -8.32 | 3.23 | 2.62 | 8.86 | -3.43 | -16.92 | -8.74 | -4.49 | 13.03 |
Nucleolus | 3.54 | 2.03 | 9.65 | 5.95 | 1.15 | -2.90 | 8.61 | 4.57 | 3.69 | -10.42 | -2.39 | 4.13 | 11.16 | 4.46 | 0.46 |
Nucleus | 2.57 | -1.52 | 13.67 | 8.36 | 12.14 | 3.28 | 13.82 | 5.48 | 2.81 | -3.86 | 3.37 | 7.39 | 16.56 | 11.25 | 7.90 |
Peroxisomes | -1.50 | 16.46 | 4.36 | 5.34 | -14.11 | -8.09 | 5.57 | -10.47 | -7.44 | -11.71 | -6.62 | 16.56 | -6.55 | -1.43 | -15.19 |
Vacuole | -3.13 | -5.78 | -6.49 | -2.87 | -1.30 | 6.02 | -0.36 | 3.33 | -0.48 | 3.18 | 1.91 | -5.20 | -5.90 | -6.79 | -1.78 |
Vacuole Periphery | -2.05 | 12.68 | 0.20 | 1.13 | -2.91 | -5.49 | 7.95 | 5.29 | 7.13 | -4.22 | -5.58 | 14.27 | 0.17 | 3.73 | -3.16 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|