Standard name
Human Ortholog
Description Scaffold subunit of the Hrd1p ubiquitin ligase; also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.08 0.08 0.13 0.1 0.17 0.18 0.21
Bud 0.07 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.09 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0.08 0.07 0 0 0 0 0 0 0 0.08 0 0 0.09 0.07 0 0 0.05 0.08 0.11 0.14 0.14
Cytoplasm 0.52 0.67 0.92 0.87 0.84 0.84 0.84 0.92 0.77 0.66 0.61 0.65 0.86 0.91 0.96 0.75 0.7 0.62 0.14 0.2 0.19 0.13 0.07 0.07
Endoplasmic Reticulum 0.37 0.21 0 0 0 0.05 0 0 0 0 0 0 0.1 0.06 0 0.08 0 0.12 0.47 0.27 0.2 0.23 0.26 0.24
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0 0.12 0.1 0.13 0.18 0.13 0.36 0.39 0.61 0.5 0 0 0 0.12 0.05 0.15 0.14 0.19 0.29 0.21 0.18 0.15
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.12 0.06 0.05 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 16 3 2 1 2 2 0 0 0 0 0 1 2 1 2 16 2 10 36 19 24 51 47
Bud 6 10 2 1 2 1 4 1 0 0 4 0 2 1 1 0 7 0 1 2 2 1 3 1
Bud Neck 1 1 2 0 1 1 0 3 0 0 0 0 0 0 1 0 4 0 2 7 5 4 11 13
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 7 10 10 11 3 3 4 0 3 0 1 12 5 8 6 6 1 5 13 16 16 40 31
Cytoplasm 45 142 153 113 137 139 203 216 92 85 49 30 126 199 272 49 57 16 17 57 38 18 20 16
Endoplasmic Reticulum 32 45 2 2 7 8 7 1 3 4 0 0 15 14 4 5 2 3 60 77 39 32 73 55
Endosome 0 1 0 0 0 2 2 0 0 0 0 0 2 0 0 0 0 2 2 9 2 1 4 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 1 0 1 4
Mitochondria 12 7 5 16 17 22 44 31 43 50 49 23 3 3 1 8 4 4 17 55 57 29 49 34
Nucleus 0 2 0 1 0 1 3 2 3 2 1 0 0 0 2 2 3 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 3 0 0 0 1 1 0 0 0 0 1 2 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 1 0 1 1 1 2 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 1
Vac/Vac Membrane 0 6 2 1 2 1 1 2 1 2 0 0 0 1 2 3 2 3 7 12 7 4 14 11
Unique Cell Count 87 212 167 130 163 166 241 236 120 128 80 46 146 218 283 65 81 26 128 285 199 141 280 229
Labelled Cell Count 103 237 180 146 179 181 272 262 143 150 104 54 161 227 294 76 101 31 128 285 199 141 280 229


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.9 5.3 5.1 5.5 4.4 4.6 4.8 4.3 4.3 3.8 3.9 5.1 5.2 5.1 6.7 6.9 6.3 4.7 4.9 5.5
Std Deviation (1e-4) 0.6 0.7 0.7 1.4 1.1 1.2 1.4 1.7 1.9 1.9 1.7 0.8 1.1 1.3 1.5 1.5 1.6 1.4 1.1 1.6 1.8
Intensity Change (Log2) -0.04 0.05 -0.27 -0.21 -0.14 -0.3 -0.28 -0.48 -0.43 -0.04 -0.02 -0.03 0.35 0.38 0.25 -0.17 -0.1 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 5.0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.6 0.3 0 0 0 0 0 0 0 0.8 -1.9 -1.7 0 0.4 0
Cytoplasm -1.3 -2.1 -2.2 -2.2 0 -3.5 -5.4 -5.8 -4.6 -1.5 -0.1 2.0 -3.3 -4.4 -4.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 3.5 2.5 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.1 2.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7841 3.2328 2.8649 2.8599 2.7965 3.1198 1.575 1.5217 1.4842 1.0327 1.7453 1.3501 0.7209 0.2326 0.3163 -0.3114 0.0307 0.6181
Actin 0.032 0.0217 0.0158 0.0259 0.0222 0.0325 0.0529 0.0157 0.0532 0.0256 0.029 0.0182 0.0624 0.015 0.0332 0.0038 0.0813 0.0039
Bud 0.0005 0.0004 0.0009 0.0009 0.0003 0.001 0.0017 0.0026 0.0015 0.0019 0.0019 0.0011 0.0013 0.0002 0.0011 0.0002 0.0004 0.0004
Bud Neck 0.0027 0.0014 0.0022 0.0212 0.0031 0.0458 0.0114 0.0032 0.0131 0.0038 0.0232 0.026 0.0046 0.0009 0.0044 0.0012 0.0022 0.0317
Bud Periphery 0.001 0.001 0.0037 0.0031 0.001 0.0033 0.0055 0.0061 0.0036 0.0072 0.0048 0.0065 0.0019 0.0005 0.0029 0.0004 0.001 0.0008
Bud Site 0.0093 0.0333 0.01 0.0021 0.0008 0.0024 0.0156 0.0899 0.0304 0.0017 0.0181 0.0023 0.0347 0.0266 0.0105 0.001 0.0012 0.0115
Cell Periphery 0.011 0.0203 0.0135 0.0065 0.0092 0.009 0.0093 0.0155 0.008 0.0271 0.0046 0.0047 0.0106 0.0079 0.0052 0.0016 0.0019 0.0024
Cytoplasm 0.3538 0.4394 0.4291 0.3248 0.4427 0.4679 0.2971 0.4553 0.3616 0.3469 0.3014 0.4694 0.225 0.3435 0.4408 0.4061 0.4602 0.4642
Cytoplasmic Foci 0.0439 0.0123 0.0281 0.0417 0.0306 0.034 0.0425 0.0192 0.045 0.0164 0.072 0.0405 0.0709 0.0335 0.0251 0.0478 0.0953 0.0134
Eisosomes 0.0019 0.0013 0.0024 0.0015 0.0009 0.0014 0.0027 0.0019 0.002 0.0011 0.0015 0.0008 0.001 0.0008 0.0008 0.0005 0.0013 0.0002
Endoplasmic Reticulum 0.1236 0.0915 0.1068 0.1451 0.1303 0.087 0.1621 0.1069 0.157 0.2287 0.0433 0.1536 0.0933 0.1158 0.0907 0.0667 0.1491 0.0998
Endosome 0.029 0.0083 0.0285 0.0128 0.0244 0.016 0.0605 0.016 0.0415 0.0252 0.0985 0.0271 0.0369 0.0276 0.0231 0.0374 0.0103 0.0139
Golgi 0.0112 0.0035 0.0108 0.0096 0.0048 0.0064 0.0236 0.0053 0.0168 0.0095 0.024 0.0107 0.0085 0.0067 0.0087 0.007 0.0122 0.0035
Lipid Particles 0.0367 0.0087 0.0167 0.0091 0.0048 0.0081 0.0582 0.0134 0.0224 0.0178 0.0398 0.0129 0.0257 0.0169 0.0153 0.0163 0.024 0.0098
Mitochondria 0.0062 0.0007 0.0105 0.0067 0.0028 0.0043 0.0191 0.0031 0.0114 0.004 0.0488 0.0124 0.0071 0.0008 0.0145 0.0018 0.0095 0.0024
None 0.3034 0.3426 0.2964 0.3465 0.2905 0.2559 0.1714 0.2183 0.1976 0.2527 0.1452 0.1797 0.319 0.3934 0.307 0.3981 0.1296 0.3183
Nuclear Periphery 0.0087 0.0045 0.007 0.0044 0.0121 0.0023 0.0238 0.0033 0.0078 0.0149 0.0195 0.0061 0.0072 0.0025 0.0029 0.002 0.0039 0.0027
Nucleolus 0.001 0.0004 0.0005 0.0004 0.0004 0.0004 0.0015 0.0026 0.0017 0.0007 0.0018 0.0014 0.0051 0.0003 0.0002 0.0002 0.0002 0.0015
Nucleus 0.0034 0.0022 0.0024 0.0019 0.0081 0.003 0.005 0.0063 0.0045 0.0034 0.0039 0.0069 0.0036 0.001 0.0015 0.0011 0.0009 0.0022
Peroxisomes 0.0091 0.0004 0.0058 0.0305 0.0032 0.0133 0.014 0.0015 0.0057 0.0007 0.0738 0.0037 0.0321 0.0005 0.005 0.0009 0.0107 0.0118
Punctate Nuclear 0.0072 0.0007 0.0016 0.0008 0.0051 0.002 0.0093 0.0078 0.0046 0.0014 0.0037 0.0088 0.035 0.0012 0.001 0.0014 0.0026 0.0027
Vacuole 0.0037 0.0052 0.0065 0.0041 0.0025 0.0035 0.0101 0.0058 0.0091 0.0077 0.0318 0.0059 0.0123 0.0042 0.0051 0.0039 0.0017 0.0028
Vacuole Periphery 0.0006 0.0003 0.001 0.0006 0.0005 0.0005 0.0029 0.0004 0.0017 0.0016 0.0093 0.0013 0.0017 0.0003 0.0009 0.0005 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.4252 12.1485 11.9226 14.9071 12.2202 13.5823 14.1654 16.0459 15.7047 15.6387
Translational Efficiency 1.1119 0.6244 0.8331 0.7795 0.7616 0.8604 0.6399 0.7161 0.8289 0.778

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Scaffold subunit of the Hrd1p ubiquitin ligase; also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
Localization
Cell Percentages ER (53%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Usa1

Usa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Usa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available