Standard name
Human Ortholog
Description AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.98 0.93 0.92 0.82 0.81 0.76 0.64 0.64 0.6 0.98 0.98 0.98 0.96 0.95 0.99 0.95 0.91 0.91 0.82 0.78 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.05 0 0 0.07 0.07 0 0 0 0 0.09 0 0 0 0 0.11 0.13 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.13 0.13 0.27 0.34 0.33 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0.05 0.11 0.08 0.06 0 0 0 0 0 0 0 0.06 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 4 6 4 4 7 3 0 0 1 0 1 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 1 0 1 1 4 3 3 0 0 0 0 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 3 4 10 0 4 5 6 5 7 7 4 7 6 6 4 3 0 0 0 2 0 0
Cytoplasm 256 211 172 166 211 98 176 153 123 116 149 123 250 352 387 78 74 82 256 207 171 184 250 251
Endoplasmic Reticulum 7 3 2 2 5 1 10 10 3 4 17 15 2 5 6 3 7 2 4 1 4 23 41 21
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 2 0 1 0 1 0 0 3 0 0 0 1 0
Mitochondria 1 1 0 0 4 15 27 24 43 61 78 73 2 0 1 0 2 1 0 3 5 2 4 2
Nucleus 2 0 0 2 2 2 3 2 2 8 3 6 1 1 2 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 0 0 1 4 2 7 12 8 20 18 12 1 1 2 1 0 1 1 12 2 8 12 16
Unique Cell Count 264 216 174 169 227 107 215 190 162 180 233 204 255 359 395 81 78 83 271 228 189 226 320 300
Labelled Cell Count 269 218 177 176 237 118 232 212 190 220 283 245 263 367 405 90 88 89 271 228 189 226 320 300


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.5 10.2 10.0 9.2 9.6 7.6 8.6 8.7 7.8 7.4 7.5 7.6 11.6 12.4 12.4 18.0 18.6 17.6 10.5 10.5 11.3
Std Deviation (1e-4) 1.7 2.0 1.6 1.5 1.3 1.5 1.6 1.5 1.7 1.7 1.6 1.4 1.9 2.0 2.1 3.6 4.0 2.6 1.5 2.0 2.0
Intensity Change (Log2) – – – -0.12 -0.05 -0.39 -0.22 -0.2 -0.35 -0.44 -0.42 -0.39 0.22 0.31 0.31 0.85 0.9 0.82 0.07 0.07 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.5 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -2.8 -3.0 -5.4 -5.6 -6.4 -8.3 -8.5 -9.0 -0.7 -0.7 -0.7 -1.4 -1.9 0
Endoplasmic Reticulum 0 0 0 2.0 2.2 0 0 2.9 2.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.1 4.8 4.9 7.3 8.4 8.5 8.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.4 0 4.5 3.8 3.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.9458 11.2518 10.0882 9.198 7.9276 10.3809 6.7206 9.743 8.5441 8.5456 6.8227 8.5603 5.5303 8.4477 7.3848 7.0209 6.8453 7.36
Actin 0.0305 0.0007 0.0238 0.0001 0.1348 0.0006 0.063 0.0005 0.018 0.0471 0.0621 0.0058 0.0475 0.0014 0.0446 0.0003 0.0081 0.0004
Bud 0.0006 0.0005 0.0007 0.0008 0.0008 0.0005 0.0015 0.0004 0.0017 0.0007 0.0023 0.0003 0.0015 0.0003 0.001 0.0005 0.0005 0.0003
Bud Neck 0.0011 0.0006 0.0006 0.0006 0.0036 0.0012 0.0013 0.0002 0.0008 0.0018 0.0011 0.001 0.0015 0.0003 0.0005 0.0003 0.0005 0.0008
Bud Periphery 0.0012 0.0002 0.0005 0.0003 0.0008 0.0009 0.0014 0.0001 0.0011 0.0004 0.0037 0.0003 0.0011 0.0001 0.0007 0.0001 0.0003 0.0002
Bud Site 0.0021 0.0023 0.0019 0.0008 0.0021 0.0003 0.0067 0.0009 0.0062 0.0022 0.0055 0.0001 0.0121 0.0008 0.0011 0.0002 0.0005 0.0001
Cell Periphery 0.0016 0.0007 0.0006 0.0017 0.0004 0.0005 0.0005 0.0002 0.0003 0.0001 0.0006 0.0002 0.0005 0.0003 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.7031 0.937 0.8601 0.8509 0.6478 0.8335 0.752 0.9596 0.8553 0.7296 0.6335 0.8921 0.7411 0.9493 0.8418 0.9102 0.8535 0.8789
Cytoplasmic Foci 0.0282 0.0073 0.0128 0.0159 0.0322 0.0148 0.0257 0.0086 0.0207 0.048 0.0383 0.0137 0.033 0.011 0.0123 0.0145 0.023 0.0141
Eisosomes 0.0009 0 0.0001 0 0.001 0 0.0003 0 0.0001 0.0002 0.0005 0.0001 0.0005 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0728 0.0254 0.0139 0.0031 0.0269 0.0655 0.0217 0.0029 0.0096 0.0144 0.0085 0.0274 0.0314 0.0046 0.0158 0.0121 0.0027 0.0271
Endosome 0.0571 0.0122 0.0351 0.0706 0.0459 0.0497 0.0726 0.0122 0.045 0.0944 0.1068 0.0342 0.0767 0.0162 0.0443 0.0447 0.0419 0.0473
Golgi 0.0108 0.0024 0.0052 0.0032 0.0122 0.0059 0.015 0.0024 0.0134 0.0133 0.0439 0.0107 0.014 0.0092 0.0082 0.0022 0.0237 0.0054
Lipid Particles 0.0124 0.0001 0.0018 0.0002 0.0153 0.0002 0.0034 0.0001 0.0032 0.0029 0.0042 0.0002 0.0039 0.0001 0.0029 0.0001 0.0003 0.0001
Mitochondria 0.006 0.0008 0.0128 0.0008 0.0036 0.008 0.0058 0.0009 0.0066 0.0039 0.0324 0.0076 0.0068 0.0018 0.0036 0.0006 0.0379 0.0188
None 0.0239 0.0013 0.0058 0.0007 0.0086 0.0009 0.0025 0.0014 0.0009 0.0008 0.0014 0.0006 0.0035 0.0007 0.0017 0.0008 0.0012 0.0006
Nuclear Periphery 0.0074 0.001 0.0064 0.0012 0.0023 0.0017 0.0089 0.0004 0.0015 0.0009 0.0062 0.0008 0.0026 0.0004 0.0038 0.001 0.0004 0.0008
Nucleolus 0.0024 0 0.0001 0 0.0022 0 0.0001 0 0.0001 0 0.0004 0 0.0003 0 0.0001 0 0 0
Nucleus 0.0028 0.0008 0.0021 0.0017 0.0016 0.001 0.0035 0.0006 0.0009 0.0007 0.0022 0.0005 0.0014 0.0005 0.001 0.0009 0.0005 0.0006
Peroxisomes 0.0154 0 0.0028 0.0001 0.0272 0.0001 0.002 0.0001 0.0025 0.0141 0.0043 0.0001 0.0031 0.0002 0.0023 0 0.0008 0.0001
Punctate Nuclear 0.0042 0.0001 0.0013 0.0001 0.0172 0.0001 0.0013 0.0001 0.0009 0.0006 0.0243 0 0.0013 0 0.0018 0.0001 0.0001 0
Vacuole 0.0128 0.0056 0.0093 0.0411 0.0106 0.0103 0.0087 0.0079 0.0092 0.0166 0.0126 0.0029 0.0135 0.0023 0.0097 0.0087 0.0029 0.0027
Vacuole Periphery 0.0027 0.0008 0.0023 0.0062 0.003 0.0044 0.0019 0.0007 0.0021 0.0072 0.0055 0.0015 0.0029 0.0004 0.0025 0.0024 0.0012 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.6895 42.5686 49.6379 58.5293 42.3362 52.5787 51.8543 56.8547 59.8608 54.6118
Translational Efficiency 1.1438 1.2269 1.2457 1.0337 1.1647 1.3749 1.0673 1.0001 1.0641 0.9653

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
40 1311 184 1155 2069 1633 185 114 2109 2944 369 1269

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 307.20 1118.86 1567.30 1550.41 1155.21 1073.41 1566.33 1569.84 1139.13 1093.65 1566.81 1552.16
Standard Deviation 10.53 190.92 187.07 238.61 150.59 181.02 206.30 233.69 188.76 186.86 196.94 238.24
Intensity Change Log 2 — 1.864779 2.351031 2.335400 — -0.105954 0.439233 0.442462 — 0.584078 1.099487 1.093314

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000047 0.000306 0.001660 0.000696 0.000200 0.000248 0.001234 0.000519 0.000197 0.000274 0.001446 0.000680
Bud Neck 0.000028 0.000599 0.004160 0.001264 0.000501 0.000885 0.002736 0.002912 0.000492 0.000758 0.003446 0.001412
Bud Site 0.000054 0.027925 0.033535 0.025655 0.016286 0.023429 0.036911 0.029761 0.015979 0.025431 0.035227 0.026024
Cell Periphery 0.000119 0.000197 0.000420 0.000997 0.000162 0.000131 0.000461 0.001146 0.000161 0.000160 0.000440 0.001011
Cytoplasm 0.000049 0.813169* 0.595504* 0.875982* 0.766404* 0.770340* 0.700811* 0.645516* 0.751869* 0.789412* 0.648300* 0.855278*
Cytoplasmic Foci 0.000020 0.107953 0.110304 0.028979 0.152972 0.140326 0.111151 0.073807 0.150071 0.125910 0.110729 0.033006
Eisosomes 0.000110 0.000006 0.000012 0.000007 0.000007 0.000010 0.000011 0.000007 0.000009 0.000008 0.000012 0.000007
Endoplasmic Reticulum 0.000001 0.002112 0.003348 0.000343 0.004328 0.001667 0.000757 0.000466 0.004246 0.001865 0.002049 0.000354
Endosome 0.000002 0.006659 0.024956 0.003740 0.006771 0.009708 0.012273 0.016905 0.006642 0.008350 0.018597 0.004923
Golgi 0.000006 0.010934 0.004862 0.006969 0.014354 0.012662 0.003328 0.004699 0.014082 0.011893 0.004093 0.006765
Lipid Particles 0.000030 0.000687 0.004859 0.005383 0.000777 0.000937 0.005447 0.003984 0.000763 0.000826 0.005154 0.005258
Mitochondria 0.000012 0.000401 0.000029 0.000529 0.000592 0.000852 0.000010 0.002028 0.000581 0.000651 0.000019 0.000663
Mitotic Spindle 0.000281 0.001890 0.011362 0.001809 0.000823 0.000925 0.003664 0.008772 0.000813 0.001355 0.007503 0.002435
None 0.999028* 0.000689 0.000204 0.000262 0.000462 0.000651 0.000080 0.000061 0.019401 0.000668 0.000142 0.000244
Nuclear Periphery 0.000000 0.000021 0.000242 0.000048 0.000067 0.000021 0.000069 0.000089 0.000066 0.000021 0.000155 0.000052
Nuclear Periphery Foci 0.000032 0.000621 0.005588 0.001236 0.000409 0.000376 0.004234 0.001467 0.000402 0.000485 0.004909 0.001256
Nucleolus 0.000047 0.000081 0.000096 0.000067 0.000051 0.000294 0.000041 0.000169 0.000050 0.000199 0.000068 0.000076
Nucleus 0.000014 0.002166 0.007384 0.003900 0.002411 0.003517 0.004817 0.003253 0.002365 0.002915 0.006097 0.003842
Peroxisomes 0.000116 0.000502 0.001007 0.000550 0.000344 0.001179 0.000955 0.002632 0.000339 0.000877 0.000981 0.000737
Vacuole 0.000005 0.016935 0.185871 0.037714 0.019587 0.025441 0.109856 0.193866 0.019215 0.021653 0.147761 0.051742
Vacuole Periphery 0.000000 0.006147 0.004599 0.003870 0.012494 0.006400 0.001156 0.007939 0.012257 0.006287 0.002873 0.004236

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.32 -2.30 -5.68 -3.26 1.30 -1.08 -5.30 -2.01 -1.73 2.85 -1.81 -3.39 -4.38 -3.82 1.91
Bud Neck -12.18 -11.91 -13.10 -7.20 7.88 -2.92 -8.08 -5.11 -4.22 -0.46 -3.34 -13.12 -9.73 -6.28 7.94
Bud Site -17.80 -9.91 -17.74 0.88 2.07 -5.08 -4.71 -3.19 -1.62 0.71 -7.91 -6.85 -6.74 -0.63 2.72
Cell Periphery -1.99 -4.52 -7.69 -7.28 -4.80 2.95 -4.89 -1.97 -2.04 -1.34 0.08 -6.67 -7.87 -7.87 -5.04
Cytoplasm -132.89* -27.93 -163.08* -6.87 -13.32 -0.48 3.89 4.53 4.65 1.69 -5.31 7.54 -12.09 -8.26 -13.95
Cytoplasmic Foci -26.97 -14.23 -17.02 17.80 9.99 2.24 5.00 8.82 7.38 3.35 5.05 6.27 27.73 26.94 13.04
Eisosomes 2.37 2.21 2.34 -0.61 2.29 -3.37 -2.81 -0.03 1.52 1.59 0.84 -1.93 1.00 0.62 2.24
Endoplasmic Reticulum -4.15 -4.48 -7.22 3.49 3.97 4.25 6.46 6.97 3.90 2.03 3.91 3.74 7.22 5.37 4.40
Endosome -12.33 -9.99 -6.16 3.76 8.23 -3.55 -2.94 -2.12 -1.40 -0.84 -2.88 -7.31 2.54 4.43 8.03
Golgi -14.68 -7.79 -6.41 3.43 -1.86 1.74 19.67 9.85 6.84 -1.09 2.95 18.27 7.37 5.04 -2.66
Lipid Particles -12.21 -9.44 -5.63 -4.96 -0.39 -1.06 -7.04 -4.57 -4.22 1.34 -0.74 -10.57 -5.21 -5.10 -0.17
Mitochondria -4.87 -4.24 -1.28 -0.24 -1.24 -1.28 8.03 -0.76 -0.59 -1.14 -0.54 7.90 -0.07 0.12 -1.59
Mitotic Spindle -1.97 -4.05 -2.60 -0.03 3.37 -0.22 -2.13 -1.30 -1.28 -0.60 -1.06 -4.33 -2.03 -1.28 3.09
None 2976.36* 4613.14* 4776.75* 1.54 -0.86 -1.29 4.26 4.85 4.81 1.12 6.30 6.49 6.45 2.88 -1.84
Nuclear Periphery -2.66 -5.98 -2.85 -1.54 4.45 0.92 -0.21 -0.39 -2.44 -0.39 0.91 -1.97 0.20 -1.98 4.03
Nuclear Periphery Foci -5.95 -7.44 -10.31 -4.33 5.76 0.16 -6.53 -3.72 -4.64 4.50 -0.48 -9.09 -4.37 -6.73 7.59
Nucleolus -0.87 -1.76 -0.71 0.52 3.00 -1.54 0.69 -3.65 0.79 -4.11 -1.67 -1.75 -2.19 1.40 -0.64
Nucleus -12.23 -9.58 -11.33 -5.65 3.67 -2.09 -5.69 -1.77 -0.34 1.57 -1.62 -8.30 -5.37 -3.70 3.27
Peroxisomes -8.35 -5.20 -9.27 -0.99 2.57 -2.36 -4.37 -2.26 -1.24 -1.61 -2.71 -5.75 -4.12 0.74 1.72
Vacuole -13.09 -11.22 -17.10 -9.10 8.76 -3.20 -7.97 -8.27 -8.00 -3.39 -1.67 -12.61 -10.97 -10.31 8.87
Vacuole Periphery -9.50 -6.11 -5.33 2.67 0.54 5.72 12.27 1.92 -0.18 -2.11 6.02 9.96 7.43 2.99 -1.73
Standard name
Human Ortholog
Description AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Amd1

Amd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Amd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available