ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.58 0.71 0.78 0.81 0.86 0.86 0.86 – 0.74 0.76 0.65 0.71 0.59 0.85 0.93 0.53 0.52 0.61 0.45 0.51 0.53 0.64 0.73 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0.1 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.11 0 0.06 0 0.16 0.1 – 0.32 0.27 0.41 0.36 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.14 0.05 0.06 0.07 0 0 0 – 0 0.05 0 0 0.14 0 0 0.2 0.17 0.17 0.27 0.2 0.18 0.12 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.35 0.24 0.16 0.1 0.15 0 0.06 – 0.05 0 0 0 0.33 0.17 0.08 0.34 0.37 0.34 0.16 0.15 0.16 0.11 0.09 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 0 1 0 0 0 1 0 0 3 1 3 0 1 2
Bud 0 0 0 1 0 2 2 – 6 6 8 10 0 1 0 1 1 0 8 3 3 2 10 9
Bud Neck 1 0 4 2 1 1 3 – 4 5 3 5 0 0 0 1 1 0 3 2 2 1 2 7
Bud Site 0 0 0 0 0 0 0 – 0 0 2 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 2 1 2 – 0 3 2 3 1 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 222 149 203 232 335 319 534 – 337 378 335 448 90 164 265 103 73 94 211 127 176 78 190 195
Endoplasmic Reticulum 0 1 1 0 0 1 0 – 0 0 0 0 0 0 0 12 4 3 2 0 1 1 2 1
Endosome 10 0 4 3 12 2 15 – 4 4 4 7 15 6 7 10 3 2 9 3 2 0 1 2
Golgi 2 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 1 0 2 1 0 2 2 3
Mitochondria 16 24 4 18 8 59 60 – 145 133 211 229 0 0 0 3 5 8 6 7 10 1 6 6
Nucleus 53 11 16 19 9 10 11 – 10 23 13 16 22 4 2 39 24 26 125 48 59 14 10 6
Nuclear Periphery 1 0 0 0 0 0 0 – 0 1 2 4 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 1 2 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 1 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 5 2 18 – 5 5 5 11 0 0 0 0 0 0 13 8 8 0 4 9
Vac/Vac Membrane 135 50 42 29 57 8 36 – 22 16 15 20 51 33 23 66 52 52 72 36 52 13 23 10
Unique Cell Count 383 211 259 287 389 372 624 453 500 513 631 153 193 284 194 140 155 468 249 336 122 262 263
Labelled Cell Count 440 236 277 306 430 405 682 533 575 602 759 179 208 297 236 165 186 468 249 336 122 262 263


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.1 5.4 4.9 5.5 3.9 4.4 – 3.8 3.9 3.7 3.8 6.0 5.3 5.3 5.7 6.0 6.0 4.8 4.8 5.2
Std Deviation (1e-4) 0.7 0.8 1.7 2.0 1.8 2.3 1.7 – 1.3 1.2 1.3 1.1 3.1 1.7 1.6 1.0 1.0 1.0 1.6 1.8 2.7
Intensity Change (Log2) – – – -0.13 0.03 -0.45 -0.28 – -0.52 -0.45 -0.53 -0.51 0.17 -0.02 -0.04 0.09 0.15 0.15 -0.17 -0.16 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.4 4.7 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4444 -0.1311 -0.3129 -0.4552 -0.6358 -0.4104 1.2629 1.24 0.9036 1.4465 0.8475 0.8908 2.1197 3.0868 2.5897 2.3727 2.1222 2.5258
Actin 0.012 0.0005 0.0085 0.0002 0.0173 0.005 0.0342 0.0004 0.0091 0.0217 0.0137 0.0027 0.0089 0.0002 0.0004 0.0006 0.0136 0.0022
Bud 0.001 0.0017 0.0003 0 0.0003 0.0002 0.002 0.0007 0.0006 0.0005 0.0002 0.0008 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001
Bud Neck 0.0041 0.0009 0.0009 0.0004 0.0051 0.0034 0.0047 0.0025 0.001 0.0467 0.0009 0.008 0.0054 0.0006 0.0004 0.0009 0.0009 0.0029
Bud Periphery 0.0016 0.0005 0.0002 0 0.0004 0.0002 0.0028 0.0006 0.0004 0.0007 0.0004 0.0015 0.0002 0.0001 0.0001 0 0.0003 0.0001
Bud Site 0.0128 0.0046 0.0025 0.0004 0.0032 0.0003 0.0067 0.0055 0.0019 0.0019 0.0009 0.0011 0.0044 0.0013 0.0004 0.0003 0.0005 0.0002
Cell Periphery 0.0008 0.0001 0.0001 0 0.0001 0 0.0004 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0 0 0
Cytoplasm 0.2868 0.2725 0.1746 0.1514 0.2058 0.2032 0.2918 0.3679 0.239 0.1173 0.2015 0.2895 0.4091 0.3211 0.2267 0.3397 0.307 0.3514
Cytoplasmic Foci 0.0292 0.0067 0.0083 0.0039 0.023 0.0079 0.0378 0.0054 0.0046 0.0175 0.0036 0.0084 0.0111 0.0045 0.0025 0.0029 0.0144 0.0046
Eisosomes 0.0004 0.0001 0.0001 0 0.0002 0.0001 0.0003 0 0 0.0001 0.002 0 0.0025 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0128 0.0096 0.0052 0.0021 0.0065 0.0031 0.0116 0.0057 0.0031 0.0018 0.0028 0.0034 0.0067 0.0044 0.0029 0.0032 0.0022 0.0032
Endosome 0.0621 0.0093 0.0184 0.0029 0.022 0.0134 0.0574 0.005 0.0066 0.0183 0.0046 0.0127 0.0129 0.004 0.0021 0.003 0.0052 0.0044
Golgi 0.0139 0.0002 0.0055 0.0001 0.0136 0.0047 0.024 0.0001 0.0025 0.0095 0.0015 0.0037 0.001 0.0001 0.0001 0.0001 0.0029 0.0006
Lipid Particles 0.0159 0.0009 0.002 0.0003 0.0226 0.0097 0.0318 0.0004 0.0006 0.0104 0.0016 0.0026 0.0016 0.0003 0.0002 0.0004 0.004 0.0006
Mitochondria 0.0297 0.0008 0.0059 0.0004 0.0305 0.0019 0.0167 0.0005 0.0029 0.0025 0.0189 0.007 0.002 0.0005 0.001 0.0008 0.0024 0.0008
None 0.0903 0.2279 0.1379 0.1579 0.0786 0.1345 0.1255 0.1994 0.1288 0.0512 0.1103 0.0972 0.218 0.1949 0.2118 0.2084 0.1771 0.1874
Nuclear Periphery 0.0437 0.0462 0.038 0.0102 0.0426 0.0216 0.0363 0.0192 0.0199 0.0083 0.0146 0.0229 0.0156 0.0114 0.0121 0.0087 0.0133 0.0076
Nucleolus 0.0037 0.0027 0.0019 0.002 0.0015 0.0027 0.0026 0.0017 0.0016 0.003 0.0027 0.0035 0.0023 0.0017 0.0015 0.0011 0.0015 0.0021
Nucleus 0.3088 0.3796 0.5482 0.6033 0.4665 0.5564 0.249 0.3548 0.5516 0.6088 0.588 0.5016 0.2652 0.4365 0.5261 0.4177 0.3945 0.4052
Peroxisomes 0.0108 0.0002 0.0086 0.0001 0.0092 0.0022 0.0329 0.0001 0.0027 0.0344 0.0012 0.0011 0.002 0.0001 0.0001 0.0001 0.0171 0.0002
Punctate Nuclear 0.0365 0.0303 0.0295 0.0634 0.0484 0.0271 0.0172 0.0263 0.02 0.0432 0.0286 0.0271 0.0275 0.0149 0.0099 0.0102 0.0407 0.0244
Vacuole 0.0166 0.0039 0.0026 0.0009 0.0016 0.0017 0.0095 0.0032 0.0025 0.0015 0.0013 0.0036 0.0027 0.0028 0.0012 0.0012 0.0018 0.0015
Vacuole Periphery 0.0063 0.0009 0.0009 0.0002 0.001 0.0006 0.0046 0.0006 0.0006 0.0004 0.0005 0.0013 0.0005 0.0003 0.0002 0.0002 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.6775 9.2617 16.0361 22.1067 13.433 10.3549 18.7291 18.9613 15.3963 23.5935
Translational Efficiency 2.7273 3.8637 1.5754 0.827 2.9416 4.1016 2.3321 2.1294 2.5849 2.5304

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
906 1209 1713 1951 1162 2567 1494 2002 2068 3776 3207 3953

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 580.84 759.50 934.01 851.72 684.33 783.62 938.73 952.36 638.99 775.90 936.21 902.69
Standard Deviation 75.18 109.07 107.91 112.22 77.12 97.27 119.76 119.69 91.95 101.82 113.61 126.50
Intensity Change Log 2 — 0.386909 0.685297 0.552238 — 0.195462 0.456018 0.476815 — 0.286519 0.565819 0.511932

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000096 0.001066 0.000363 0.000356 0.000054 0.000319 0.000430 0.000372 0.000072 0.000558 0.000394 0.000364
Bud Neck 0.007145 0.014229 0.007479 0.011350 0.004424 0.018152 0.005105 0.009638 0.005616 0.016896 0.006373 0.010483
Bud Site 0.002274 0.007975 0.005585 0.019059 0.001239 0.007830 0.004252 0.022879 0.001692 0.007877 0.004964 0.020994
Cell Periphery 0.000347 0.000208 0.000102 0.000151 0.000109 0.000159 0.000135 0.000179 0.000214 0.000175 0.000117 0.000165
Cytoplasm 0.372184* 0.371848* 0.093021 0.301674* 0.303043* 0.383906* 0.207119* 0.350183* 0.333334* 0.380045* 0.146174* 0.326241*
Cytoplasmic Foci 0.162729 0.076838 0.000861 0.002888 0.055564 0.040317 0.001358 0.004053 0.102513 0.052010 0.001092 0.003478
Eisosomes 0.000201 0.000063 0.000018 0.000022 0.000042 0.000036 0.000022 0.000020 0.000112 0.000045 0.000020 0.000021
Endoplasmic Reticulum 0.001323 0.002778 0.004441 0.004083 0.000747 0.000901 0.005648 0.002669 0.000999 0.001502 0.005003 0.003367
Endosome 0.003850 0.006128 0.000714 0.001049 0.001706 0.002922 0.000938 0.002378 0.002646 0.003949 0.000818 0.001722
Golgi 0.000977 0.001362 0.000118 0.000074 0.000364 0.000798 0.000073 0.001463 0.000633 0.000978 0.000097 0.000778
Lipid Particles 0.013859 0.004428 0.000401 0.000490 0.003018 0.002079 0.000590 0.000498 0.007767 0.002831 0.000489 0.000494
Mitochondria 0.009291 0.005021 0.001378 0.001459 0.001507 0.005461 0.001148 0.002538 0.004917 0.005320 0.001271 0.002005
Mitotic Spindle 0.000296 0.004469 0.005164 0.005826 0.003624 0.007969 0.012168 0.018290 0.002166 0.006848 0.008427 0.012138
None 0.014288 0.009775 0.003622 0.009291 0.004434 0.007736 0.006607 0.010115 0.008751 0.008389 0.005013 0.009708
Nuclear Periphery 0.000778 0.001296 0.002082 0.001895 0.001428 0.001632 0.002171 0.002075 0.001143 0.001524 0.002124 0.001986
Nuclear Periphery Foci 0.000378 0.000974 0.000455 0.000807 0.000270 0.000238 0.001024 0.000690 0.000317 0.000474 0.000720 0.000748
Nucleolus 0.002793 0.003037 0.000520 0.000443 0.001987 0.001891 0.000333 0.000640 0.002340 0.002258 0.000433 0.000543
Nucleus 0.388951* 0.457974* 0.860945* 0.602828* 0.606212* 0.498075* 0.723785* 0.536716* 0.511029* 0.485235* 0.797048* 0.569345*
Peroxisomes 0.005584 0.004170 0.000126 0.000207 0.002178 0.002639 0.000080 0.000171 0.003670 0.003129 0.000104 0.000189
Vacuole 0.011857 0.025419 0.012365 0.035652 0.007847 0.016125 0.026559 0.033891 0.009604 0.019101 0.018978 0.034760
Vacuole Periphery 0.000799 0.000940 0.000240 0.000397 0.000204 0.000813 0.000455 0.000542 0.000464 0.000854 0.000340 0.000471

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.32 -9.44 -9.82 0.85 1.41 -1.54 -15.23 -14.27 -0.54 4.78 -1.85 -17.16 -16.82 0.49 4.14
Bud Neck -4.02 -2.16 -3.75 1.73 -1.48 -9.59 -3.75 -5.55 4.86 -2.53 -10.07 -4.31 -6.80 5.13 -2.71
Bud Site -4.59 -3.35 -6.23 -2.21 -3.82 -7.89 -4.69 -7.11 -2.62 -4.34 -8.89 -5.65 -9.48 -3.49 -5.73
Cell Periphery 3.44 9.23 8.51 2.45 -2.18 -4.43 -2.83 -0.63 2.56 1.16 2.29 7.26 6.47 3.67 -0.30
Cytoplasm 0.18 31.59 13.79 14.32 -24.91 -8.37 13.94 4.21 16.14 -12.82 -6.25 31.53 12.17 22.52 -26.44
Cytoplasmic Foci 11.97 27.03 26.64 19.72 -7.68 4.40 17.53 16.97 21.39 -4.34 13.32 30.29 29.68 28.73 -8.30
Eisosomes 8.07 10.58 10.19 8.20 -3.51 2.30 4.52 5.33 2.70 0.29 8.65 11.03 10.65 7.17 -2.48
Endoplasmic Reticulum -4.56 -11.06 -11.89 -7.54 2.01 -1.19 -19.36 -19.85 -15.81 8.25 -3.40 -20.72 -20.71 -17.71 6.72
Endosome -2.09 4.43 4.19 5.71 -1.39 -3.17 3.59 1.45 4.28 -1.66 -2.73 5.49 4.46 7.44 -2.16
Golgi -1.01 5.52 5.83 3.72 0.64 -1.71 1.97 -0.39 1.00 -1.60 -1.67 5.00 2.10 3.16 -1.48
Lipid Particles 6.67 10.01 9.83 8.50 -4.39 2.80 7.75 7.99 8.70 0.82 7.62 11.60 11.44 12.01 -2.65
Mitochondria 2.29 4.46 4.62 4.34 1.74 -4.57 0.25 -0.55 4.61 -1.36 -0.43 4.24 4.09 6.31 -0.40
Mitotic Spindle -3.17 -6.16 -6.67 -2.18 0.80 -2.38 -4.17 -6.25 -4.45 -1.93 -3.89 -6.60 -8.21 -4.43 -1.25
None 2.31 5.72 3.63 1.98 -6.42 -3.88 -2.50 -7.58 -1.58 -6.05 0.44 4.69 0.56 0.08 -9.07
Nuclear Periphery -5.65 -18.58 -19.27 -14.12 -1.35 -0.45 -6.07 -7.84 -4.97 -3.10 -1.31 -11.76 -13.87 -7.03 -3.15
Nuclear Periphery Foci -5.41 -2.03 -5.70 -1.13 -3.51 0.74 -6.09 -6.81 -7.49 1.26 -3.25 -5.88 -8.54 -6.28 -1.95
Nucleolus -0.51 4.75 4.73 7.36 -0.09 0.62 2.95 2.26 4.84 -2.64 0.55 4.86 4.31 8.93 -2.23
Nucleus -5.35 -43.76 -21.72 -16.36 27.24 9.79 -11.58 1.44 -10.53 16.23 3.00 -35.32 -12.19 -18.86 30.66
Peroxisomes 1.75 8.43 8.37 9.99 -0.85 -0.78 3.94 3.92 6.66 -0.87 0.90 8.58 8.52 10.29 -1.04
Vacuole -5.66 -4.48 -16.71 -12.53 -13.80 -6.23 -13.88 -22.40 -18.63 -8.17 -7.40 -13.67 -27.75 -22.88 -15.70
Vacuole Periphery -0.81 4.23 3.62 5.24 -0.55 -4.16 -0.73 -0.93 3.77 0.38 -3.25 2.32 2.88 5.38 0.01
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene
Localization
Cell Percentages nucleus (40%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

YML053C

YML053C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yml053c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available