Standard name | |
---|---|
Human Ortholog | |
Description | Protein localized to COPII-coated vesicles; forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 16 | 8 | 23 | 9 | 15 | 22 | 13 | 21 | – | 19 | 17 | 36 | 35 | 25 | 23 | 9 | 11 | 7 | 15 | 4 | 6 | 15 | 10 | 13 |
Bud | 3 | 0 | 2 | 0 | 9 | 8 | 10 | 15 | – | 16 | 21 | 14 | 8 | 1 | 0 | 2 | 2 | 1 | 2 | 0 | 0 | 2 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 7 | 4 | 18 | 12 | 19 | 38 | – | 23 | 29 | 27 | 8 | 32 | 66 | 18 | 15 | 18 | 1 | 0 | 3 | 1 | 3 | 4 |
Cytoplasm | 44 | 20 | 42 | 35 | 81 | 74 | 113 | 173 | – | 107 | 97 | 95 | 38 | 45 | 53 | 48 | 41 | 45 | 6 | 2 | 7 | 10 | 13 | 11 |
Endoplasmic Reticulum | 264 | 122 | 191 | 93 | 61 | 154 | 119 | 93 | – | 92 | 74 | 165 | 289 | 349 | 396 | 1 | 3 | 0 | 283 | 128 | 221 | 309 | 277 | 289 |
Endosome | 2 | 0 | 5 | 1 | 4 | 5 | 11 | 16 | – | 15 | 2 | 3 | 0 | 1 | 2 | 5 | 0 | 0 | 3 | 1 | 0 | 3 | 1 | 4 |
Golgi | 22 | 2 | 10 | 12 | 12 | 0 | 2 | 3 | – | 0 | 0 | 2 | 7 | 10 | 7 | 6 | 3 | 4 | 6 | 2 | 3 | 10 | 6 | 4 |
Mitochondria | 3 | 1 | 5 | 5 | 18 | 39 | 68 | 96 | – | 105 | 149 | 167 | 0 | 0 | 0 | 14 | 7 | 1 | 3 | 0 | 2 | 7 | 6 | 7 |
Nucleus | 1 | 0 | 0 | 0 | 1 | 1 | 5 | 4 | – | 2 | 3 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 3 | 4 | 2 | 2 | 3 | 4 | 3 | – | 3 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 11 | 4 | 9 | 21 | 88 | 32 | 59 | 117 | – | 68 | 43 | 33 | 9 | 14 | 9 | 31 | 8 | 6 | 3 | 2 | 0 | 11 | 4 | 6 |
Unique Cell Count | 321 | 139 | 241 | 152 | 248 | 290 | 338 | 476 | 370 | 347 | 420 | 342 | 409 | 473 | 108 | 79 | 78 | 331 | 142 | 250 | 383 | 330 | 356 | |
Labelled Cell Count | 368 | 163 | 298 | 182 | 309 | 351 | 425 | 581 | 456 | 439 | 548 | 395 | 479 | 557 | 137 | 93 | 82 | 331 | 142 | 250 | 383 | 330 | 356 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.6 | 18.2 | 13.3 | 12.3 | 12.5 | 9.9 | 9.1 | 9.8 | – | 9.0 | 8.5 | 8.8 | 15.1 | 15.4 | 14.8 | 10.3 | 10.3 | 10.7 | 16.0 | 17.1 | 16.5 |
Std Deviation (1e-4) | 4.1 | 3.2 | 2.8 | 2.4 | 5.8 | 2.7 | 2.5 | 2.7 | – | 2.4 | 2.1 | 2.2 | 2.9 | 2.9 | 2.6 | 2.0 | 2.0 | 2.1 | 3.4 | 3.5 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.09 | -0.42 | -0.54 | -0.44 | – | -0.57 | -0.64 | -0.6 | 0.18 | 0.22 | 0.16 | -0.37 | -0.37 | -0.3 | 0.27 | 0.37 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.3 | -1.4 | -0.8 | -2.8 | -2.7 | – | -2.1 | -2.2 | -0.4 | 0.3 | -1.6 | -2.4 | -0.4 | 1.1 | -0.1 |
Bud | 0 | 2.1 | 0 | 0 | 1.9 | – | 2.5 | 3.2 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 2.2 | 0.8 | 1.6 | 2.6 | – | 1.9 | 2.7 | 2.0 | -0.4 | 2.6 | 4.6 | 4.6 | 4.9 | 5.8 |
Cytoplasm | 1.4 | 3.9 | 2.2 | 4.3 | 5.2 | – | 3.2 | 3.0 | 1.6 | -2.2 | -2.3 | -2.3 | 5.3 | 6.1 | 6.9 |
Endoplasmic Reticulum | -3.9 | -12.1 | -6.3 | -10.5 | -15.4 | – | -13.2 | -13.9 | -9.9 | 1.6 | 2.0 | 1.5 | -13.6 | -11.9 | -12.4 |
Endosome | 0 | 0 | 0 | 0.9 | 1.0 | – | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.6 | 0.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.5 | -1.2 | -2.2 | 0 | 0 | 0 |
Mitochondria | 0 | 2.7 | 4.7 | 6.4 | 6.6 | – | 8.3 | 11.1 | 10.6 | 0 | 0 | 0 | 4.1 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.7 | 8.8 | 3.1 | 5.1 | 6.9 | – | 5.3 | 3.6 | 2.1 | -0.8 | -0.2 | -1.5 | 6.8 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9128 | 3.9231 | 2.7877 | 2.8534 | 2.4455 | 4.486 | 5.5041 | 5.4463 | 5.8215 | 4.9703 | 5.6248 | 5.616 | 4.5519 | 4.4084 | 4.4161 | 4.0716 | 3.8891 | 4.5464 |
Actin | 0.0401 | 0.0078 | 0.0078 | 0.0119 | 0.0161 | 0.0073 | 0.0321 | 0.0142 | 0.0283 | 0.0287 | 0.0271 | 0.0117 | 0.0094 | 0.0096 | 0.0151 | 0.025 | 0.0053 | 0.0112 |
Bud | 0.0011 | 0.0071 | 0.002 | 0.012 | 0.001 | 0.0019 | 0.0012 | 0.0078 | 0.0037 | 0.0009 | 0.001 | 0.0005 | 0.0006 | 0.006 | 0.0036 | 0.0026 | 0.0004 | 0.0021 |
Bud Neck | 0.0058 | 0.0004 | 0.0008 | 0.0014 | 0.0007 | 0.0015 | 0.0013 | 0.0005 | 0.0007 | 0.0007 | 0.001 | 0.0049 | 0.0002 | 0.0007 | 0.0005 | 0.0003 | 0.0006 | 0.004 |
Bud Periphery | 0.0014 | 0.0088 | 0.0037 | 0.0112 | 0.0008 | 0.0023 | 0.004 | 0.0195 | 0.0122 | 0.0096 | 0.003 | 0.0016 | 0.0039 | 0.0084 | 0.0126 | 0.0172 | 0.0017 | 0.0106 |
Bud Site | 0.0243 | 0.0164 | 0.0052 | 0.0032 | 0.008 | 0.0006 | 0.0055 | 0.0065 | 0.0066 | 0.0006 | 0.0012 | 0.0005 | 0.0014 | 0.0248 | 0.0043 | 0.0008 | 0.0007 | 0.0004 |
Cell Periphery | 0.0022 | 0.0033 | 0.0013 | 0.0038 | 0.0003 | 0.0008 | 0.0025 | 0.0035 | 0.0021 | 0.0041 | 0.0011 | 0.0008 | 0.0022 | 0.0027 | 0.0025 | 0.0027 | 0.0019 | 0.0016 |
Cytoplasm | 0.1352 | 0.2078 | 0.2738 | 0.194 | 0.1849 | 0.2279 | 0.1653 | 0.2521 | 0.1651 | 0.2148 | 0.1342 | 0.219 | 0.1519 | 0.2137 | 0.1627 | 0.0803 | 0.1777 | 0.1721 |
Cytoplasmic Foci | 0.0678 | 0.0246 | 0.0295 | 0.0356 | 0.0345 | 0.0173 | 0.0375 | 0.0121 | 0.0235 | 0.014 | 0.0383 | 0.0222 | 0.0186 | 0.0244 | 0.0225 | 0.024 | 0.0147 | 0.02 |
Eisosomes | 0.0004 | 0.0005 | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0.0008 | 0.0003 | 0.0004 | 0.0006 | 0.0004 | 0.0002 | 0.0005 | 0.0003 | 0.0005 | 0.0008 | 0.0005 | 0.0003 |
Endoplasmic Reticulum | 0.3737 | 0.3883 | 0.3693 | 0.309 | 0.3166 | 0.5515 | 0.3511 | 0.3467 | 0.4038 | 0.3339 | 0.3673 | 0.4327 | 0.5431 | 0.4057 | 0.4911 | 0.5584 | 0.4754 | 0.5254 |
Endosome | 0.089 | 0.0406 | 0.0348 | 0.0629 | 0.0793 | 0.0729 | 0.0661 | 0.0348 | 0.0567 | 0.0493 | 0.0888 | 0.0557 | 0.031 | 0.04 | 0.0477 | 0.0346 | 0.061 | 0.0348 |
Golgi | 0.0277 | 0.01 | 0.0165 | 0.0189 | 0.0604 | 0.0112 | 0.0171 | 0.0086 | 0.0167 | 0.0281 | 0.0469 | 0.0168 | 0.0086 | 0.0142 | 0.0116 | 0.0226 | 0.0115 | 0.0083 |
Lipid Particles | 0.0289 | 0.0115 | 0.0148 | 0.034 | 0.0113 | 0.0075 | 0.0267 | 0.0152 | 0.0253 | 0.0052 | 0.0237 | 0.0121 | 0.0214 | 0.0292 | 0.0193 | 0.0198 | 0.0218 | 0.0139 |
Mitochondria | 0.0167 | 0.0066 | 0.0128 | 0.0427 | 0.0475 | 0.0036 | 0.0111 | 0.0042 | 0.0038 | 0.0029 | 0.0065 | 0.003 | 0.0024 | 0.0025 | 0.0059 | 0.0154 | 0.0032 | 0.0028 |
None | 0.0987 | 0.2314 | 0.2017 | 0.2358 | 0.1947 | 0.0727 | 0.2306 | 0.225 | 0.2151 | 0.2705 | 0.212 | 0.1956 | 0.1743 | 0.1629 | 0.1532 | 0.1543 | 0.1783 | 0.1588 |
Nuclear Periphery | 0.022 | 0.0186 | 0.0085 | 0.0085 | 0.0086 | 0.0097 | 0.0156 | 0.0235 | 0.0169 | 0.0175 | 0.021 | 0.0104 | 0.0167 | 0.0258 | 0.0244 | 0.0203 | 0.0305 | 0.0181 |
Nucleolus | 0.0028 | 0.0012 | 0.0004 | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0011 | 0.0004 | 0.0003 | 0.0001 | 0.0003 |
Nucleus | 0.0285 | 0.0053 | 0.0023 | 0.0023 | 0.0032 | 0.0028 | 0.0026 | 0.0066 | 0.0033 | 0.0021 | 0.005 | 0.0032 | 0.0024 | 0.0101 | 0.0049 | 0.0022 | 0.0035 | 0.0038 |
Peroxisomes | 0.0099 | 0.0007 | 0.0057 | 0.0026 | 0.0204 | 0.0003 | 0.0086 | 0.0008 | 0.0041 | 0.0005 | 0.005 | 0.0009 | 0.0007 | 0.0009 | 0.0009 | 0.0034 | 0.0003 | 0.0007 |
Punctate Nuclear | 0.0097 | 0.0037 | 0.0033 | 0.0023 | 0.0024 | 0.0024 | 0.008 | 0.0016 | 0.002 | 0.002 | 0.004 | 0.0017 | 0.0021 | 0.0035 | 0.0023 | 0.0017 | 0.002 | 0.0028 |
Vacuole | 0.011 | 0.0044 | 0.0042 | 0.0054 | 0.0067 | 0.004 | 0.0093 | 0.0141 | 0.0073 | 0.0104 | 0.0099 | 0.0049 | 0.007 | 0.0119 | 0.0109 | 0.0097 | 0.0063 | 0.0059 |
Vacuole Periphery | 0.0032 | 0.0009 | 0.0008 | 0.0021 | 0.0017 | 0.0014 | 0.0026 | 0.0021 | 0.0022 | 0.0035 | 0.0024 | 0.0014 | 0.0015 | 0.0018 | 0.0031 | 0.0036 | 0.0026 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.1563 | 32.5998 | 31.102 | 41.1408 | 31.0709 | 28.1166 | 35.5514 | 41.6334 | 38.4877 | 47.3621 |
Translational Efficiency | 0.8807 | 0.9552 | 1.1782 | 0.771 | 1.1338 | 1.3524 | 1.1256 | 0.7725 | 1.0122 | 0.8357 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2281 | 2049 | 118 | 654 | 2221 | 68 | 596 | 142 | 4502 | 2117 | 714 | 796 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 860.96 | 1073.31 | 1558.04 | 1041.07 | 808.48 | 905.08 | 910.06 | 1210.77 | 835.07 | 1067.91 | 1017.15 | 1071.34 |
Standard Deviation | 163.06 | 307.56 | 328.70 | 296.60 | 150.78 | 241.66 | 160.26 | 396.00 | 159.30 | 307.10 | 311.80 | 323.22 |
Intensity Change Log 2 | — | 0.318049 | 0.855714 | 0.274049 | — | 0.162833 | 0.170750 | 0.582641 | — | 0.244963 | 0.564037 | 0.431740 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004543 | 0.021394 | 0.005320 | 0.008868 | 0.003836 | 0.004783 | 0.011136 | 0.013492 | 0.004194 | 0.020860 | 0.010175 | 0.009693 |
Bud Neck | 0.002171 | 0.003121 | 0.004050 | 0.010541 | 0.007948 | 0.008961 | 0.014618 | 0.010220 | 0.005021 | 0.003308 | 0.012871 | 0.010483 |
Bud Site | 0.007618 | 0.039286 | 0.006740 | 0.052872 | 0.010054 | 0.026422 | 0.047097 | 0.017872 | 0.008819 | 0.038873 | 0.040427 | 0.046629 |
Cell Periphery | 0.001443 | 0.003920 | 0.001935 | 0.003707 | 0.001213 | 0.000485 | 0.001798 | 0.007873 | 0.001329 | 0.003809 | 0.001821 | 0.004450 |
Cytoplasm | 0.090350 | 0.076383 | 0.026057 | 0.219573* | 0.238843* | 0.206489* | 0.321134* | 0.114114* | 0.163607* | 0.080562 | 0.272368* | 0.200760* |
Cytoplasmic Foci | 0.068501 | 0.040406 | 0.009118 | 0.050521 | 0.133119 | 0.126583 | 0.052215 | 0.035713 | 0.100380 | 0.043174 | 0.045093 | 0.047879 |
Eisosomes | 0.000788 | 0.000543 | 0.000113 | 0.000338 | 0.000837 | 0.000224 | 0.000912 | 0.000286 | 0.000812 | 0.000533 | 0.000780 | 0.000329 |
Endoplasmic Reticulum | 0.515678* | 0.596696* | 0.692796* | 0.274886* | 0.271248* | 0.293983* | 0.118958* | 0.584266* | 0.395092* | 0.586973* | 0.213794* | 0.330077* |
Endosome | 0.034314 | 0.052756 | 0.052019 | 0.031394 | 0.038840 | 0.046753 | 0.012577 | 0.018597 | 0.036547 | 0.052563 | 0.019095 | 0.029111 |
Golgi | 0.097817 | 0.042021 | 0.008491 | 0.010238 | 0.055202 | 0.035923 | 0.003408 | 0.009064 | 0.076794 | 0.041825 | 0.004248 | 0.010028 |
Lipid Particles | 0.111864* | 0.065070 | 0.056682 | 0.036267 | 0.109380* | 0.039474 | 0.060612 | 0.052972 | 0.110638* | 0.064248 | 0.059963 | 0.039247 |
Mitochondria | 0.018754 | 0.016119 | 0.000203 | 0.006706 | 0.023630 | 0.011075 | 0.000985 | 0.000364 | 0.021159 | 0.015957 | 0.000855 | 0.005575 |
Mitotic Spindle | 0.000424 | 0.001886 | 0.005656 | 0.027861 | 0.001254 | 0.003483 | 0.010459 | 0.005946 | 0.000833 | 0.001937 | 0.009666 | 0.023951 |
None | 0.011830 | 0.007364 | 0.000091 | 0.003858 | 0.009274 | 0.000532 | 0.007948 | 0.001430 | 0.010569 | 0.007145 | 0.006649 | 0.003425 |
Nuclear Periphery | 0.000729 | 0.000283 | 0.006704 | 0.003714 | 0.003029 | 0.001153 | 0.001782 | 0.001057 | 0.001864 | 0.000311 | 0.002596 | 0.003240 |
Nuclear Periphery Foci | 0.007066 | 0.004397 | 0.066146 | 0.047827 | 0.006353 | 0.005943 | 0.027045 | 0.004515 | 0.006714 | 0.004446 | 0.033507 | 0.040100 |
Nucleolus | 0.000281 | 0.000360 | 0.000328 | 0.000473 | 0.000730 | 0.000328 | 0.000322 | 0.000216 | 0.000503 | 0.000359 | 0.000323 | 0.000427 |
Nucleus | 0.005961 | 0.007675 | 0.008947 | 0.047855 | 0.035611 | 0.043624 | 0.134318* | 0.035976 | 0.020588 | 0.008829 | 0.113598 | 0.045736 |
Peroxisomes | 0.010435 | 0.004285 | 0.000710 | 0.002768 | 0.009644 | 0.008036 | 0.003717 | 0.001561 | 0.010045 | 0.004405 | 0.003220 | 0.002553 |
Vacuole | 0.006294 | 0.012149 | 0.046526 | 0.156160 | 0.034602 | 0.134093 | 0.166687 | 0.083818 | 0.020259 | 0.016066 | 0.146829 | 0.143254 |
Vacuole Periphery | 0.003138 | 0.003887 | 0.001366 | 0.003572 | 0.005354 | 0.001655 | 0.002272 | 0.000647 | 0.004231 | 0.003815 | 0.002123 | 0.003050 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.69 | -0.35 | -1.33 | 5.30 | -0.53 | -0.20 | -0.28 | -1.05 | -0.90 | -1.00 | -9.02 | -0.17 | -1.81 | 4.59 | -1.62 |
Bud Neck | -2.04 | -1.79 | -7.57 | -6.68 | -3.81 | -0.36 | -3.48 | -0.72 | -0.45 | 0.24 | 3.45 | -6.38 | -4.61 | -6.13 | 0.20 |
Bud Site | -11.79 | -0.09 | -7.81 | -1.37 | -7.16 | -1.96 | -3.92 | -1.48 | 0.86 | 1.06 | -11.76 | -4.20 | -7.76 | -0.59 | -3.85 |
Cell Periphery | -7.20 | -1.31 | -3.04 | 2.05 | -1.57 | 3.52 | 2.27 | -2.64 | -2.97 | -2.86 | -7.60 | 2.51 | -4.13 | 0.37 | -4.86 |
Cytoplasm | 2.49 | 6.07 | -11.92 | -12.82 | -13.66 | 0.59 | 0.35 | 4.79 | 2.19 | 4.22 | 15.39 | -3.76 | -4.67 | -12.27 | -0.83 |
Cytoplasmic Foci | 9.17 | 17.26 | 5.34 | -1.33 | -8.45 | 0.49 | 24.55 | 10.36 | 3.91 | -1.19 | 20.05 | 24.25 | 14.36 | -0.49 | -4.43 |
Eisosomes | 2.93 | 8.55 | 5.61 | 3.57 | -2.73 | 6.85 | 4.42 | 6.51 | -0.69 | 1.22 | 4.31 | 5.77 | 7.96 | 3.86 | 0.73 |
Endoplasmic Reticulum | -6.75 | -4.72 | 20.25 | 24.88 | 13.48 | -0.04 | 24.30 | -6.58 | -3.98 | -12.60 | -18.40 | 19.44 | 9.30 | 20.34 | -6.25 |
Endosome | -8.78 | -2.96 | 4.13 | 10.56 | 4.43 | -0.58 | 19.15 | 5.91 | 2.81 | -3.56 | -8.15 | 14.06 | 6.53 | 11.89 | -4.45 |
Golgi | 14.31 | 22.40 | 25.08 | 13.55 | 0.38 | 1.44 | 23.09 | 15.95 | 1.67 | -3.81 | 12.02 | 35.41 | 28.87 | 14.10 | -3.85 |
Lipid Particles | 9.70 | 4.21 | 17.04 | 7.97 | 2.16 | 5.65 | 17.18 | 5.36 | -0.70 | -2.20 | 11.68 | 18.37 | 18.90 | 7.00 | -0.82 |
Mitochondria | 1.35 | 14.92 | 7.62 | 5.72 | -3.42 | 1.88 | 17.57 | 17.83 | 1.44 | 1.67 | 3.07 | 22.82 | 12.01 | 6.63 | -3.19 |
Mitotic Spindle | -3.22 | -1.77 | -6.13 | -5.76 | -3.60 | -1.06 | -4.21 | -0.96 | -0.61 | 0.59 | -2.42 | -4.69 | -6.05 | -5.71 | -3.05 |
None | 2.95 | 10.04 | 5.08 | 2.68 | -2.53 | 7.69 | 3.23 | 6.00 | -1.22 | 3.45 | 2.77 | 6.28 | 5.91 | 3.10 | 1.04 |
Nuclear Periphery | 3.88 | -4.21 | -5.69 | -6.70 | 1.99 | 3.61 | 1.28 | 3.09 | -0.01 | 3.01 | 6.49 | -3.52 | -2.77 | -6.85 | -0.21 |
Nuclear Periphery Foci | 3.66 | -5.76 | -7.27 | -7.91 | 2.38 | 0.46 | -3.52 | 1.40 | 0.50 | 4.02 | 3.88 | -6.30 | -7.18 | -7.82 | -2.88 |
Nucleolus | -0.77 | -0.22 | -2.03 | -0.70 | -0.60 | 4.11 | 4.08 | 6.39 | 0.81 | 1.51 | 0.63 | 1.46 | 0.12 | -0.48 | -0.91 |
Nucleus | -1.71 | -1.30 | -11.65 | -11.11 | -5.08 | -0.91 | -20.24 | -1.21 | -0.21 | 12.73 | 8.93 | -20.13 | -8.58 | -11.03 | 14.81 |
Peroxisomes | 7.29 | 10.93 | 9.69 | 3.82 | -2.33 | 0.83 | 11.77 | 11.66 | 2.39 | -0.23 | 9.44 | 14.54 | 13.89 | 4.60 | -1.53 |
Vacuole | -5.60 | -4.80 | -21.23 | -20.65 | -13.40 | -4.26 | -23.37 | -4.30 | 1.48 | 10.93 | 2.04 | -23.36 | -20.22 | -20.43 | 3.65 |
Vacuole Periphery | -1.60 | 3.39 | 0.85 | 1.78 | -1.64 | 8.67 | 7.53 | 15.80 | 2.71 | 0.27 | 0.93 | 6.86 | 3.89 | 2.48 | -2.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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