Standard name
Human Ortholog
Description Protein localized to COPII-coated vesicles; forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.06 0.1 0.06 0.06 0.08 0 0 0.05 0.05 0.09 0.1 0.06 0.05 0.08 0.14 0.09 0.05 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.07 0 0.06 0.08 0.06 0.08 0.06 0 0.08 0.14 0.17 0.19 0.23 0 0 0 0 0 0
Cytoplasm 0.14 0.14 0.17 0.23 0.33 0.26 0.33 0.36 0.29 0.28 0.23 0.11 0.11 0.11 0.44 0.52 0.58 0 0 0 0 0 0
Endoplasmic Reticulum 0.82 0.88 0.79 0.61 0.25 0.53 0.35 0.2 0.25 0.21 0.39 0.85 0.85 0.84 0 0 0 0.86 0.9 0.89 0.81 0.84 0.81
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0.07 0 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.13 0.2 0.2 0.28 0.43 0.4 0 0 0 0.13 0.09 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.14 0.35 0.11 0.17 0.25 0.18 0.12 0.08 0 0 0 0.29 0.1 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 8 23 9 15 22 13 21 19 17 36 35 25 23 9 11 7 15 4 6 15 10 13
Bud 3 0 2 0 9 8 10 15 16 21 14 8 1 0 2 2 1 2 0 0 2 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 0 1 0 0 2 2 2 0 0 0 0 0 0
Cell Periphery 2 3 7 4 18 12 19 38 23 29 27 8 32 66 18 15 18 1 0 3 1 3 4
Cytoplasm 44 20 42 35 81 74 113 173 107 97 95 38 45 53 48 41 45 6 2 7 10 13 11
Endoplasmic Reticulum 264 122 191 93 61 154 119 93 92 74 165 289 349 396 1 3 0 283 128 221 309 277 289
Endosome 2 0 5 1 4 5 11 16 15 2 3 0 1 2 5 0 0 3 1 0 3 1 4
Golgi 22 2 10 12 12 0 2 3 0 0 2 7 10 7 6 3 4 6 2 3 10 6 4
Mitochondria 3 1 5 5 18 39 68 96 105 149 167 0 0 0 14 7 1 3 0 2 7 6 7
Nucleus 1 0 0 0 1 1 5 4 2 3 1 1 1 1 1 2 0 0 0 0 0 0 1
Nuclear Periphery 0 3 4 2 2 3 4 3 3 2 2 0 1 0 0 0 0 2 0 0 2 1 1
Nucleolus 0 0 0 0 0 0 1 1 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 3 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Vac/Vac Membrane 11 4 9 21 88 32 59 117 68 43 33 9 14 9 31 8 6 3 2 0 11 4 6
Unique Cell Count 321 139 241 152 248 290 338 476 370 347 420 342 409 473 108 79 78 331 142 250 383 330 356
Labelled Cell Count 368 163 298 182 309 351 425 581 456 439 548 395 479 557 137 93 82 331 142 250 383 330 356


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.6 18.2 13.3 12.3 12.5 9.9 9.1 9.8 9.0 8.5 8.8 15.1 15.4 14.8 10.3 10.3 10.7 16.0 17.1 16.5
Std Deviation (1e-4) 4.1 3.2 2.8 2.4 5.8 2.7 2.5 2.7 2.4 2.1 2.2 2.9 2.9 2.6 2.0 2.0 2.1 3.4 3.5 3.5
Intensity Change (Log2) -0.1 -0.09 -0.42 -0.54 -0.44 -0.57 -0.64 -0.6 0.18 0.22 0.16 -0.37 -0.37 -0.3 0.27 0.37 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.3 -1.4 -0.8 -2.8 -2.7 -2.1 -2.2 -0.4 0.3 -1.6 -2.4 -0.4 1.1 -0.1
Bud 0 2.1 0 0 1.9 2.5 3.2 2.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.2 0.8 1.6 2.6 1.9 2.7 2.0 -0.4 2.6 4.6 4.6 4.9 5.8
Cytoplasm 1.4 3.9 2.2 4.3 5.2 3.2 3.0 1.6 -2.2 -2.3 -2.3 5.3 6.1 6.9
Endoplasmic Reticulum -3.9 -12.1 -6.3 -10.5 -15.4 -13.2 -13.9 -9.9 1.6 2.0 1.5 -13.6 -11.9 -12.4
Endosome 0 0 0 0.9 1.0 1.3 0 0 0 0 0 0 0 0
Golgi 1.6 0.4 0 0 0 0 0 0 -1.5 -1.2 -2.2 0 0 0
Mitochondria 0 2.7 4.7 6.4 6.6 8.3 11.1 10.6 0 0 0 4.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.7 8.8 3.1 5.1 6.9 5.3 3.6 2.1 -0.8 -0.2 -1.5 6.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9128 3.9231 2.7877 2.8534 2.4455 4.486 5.5041 5.4463 5.8215 4.9703 5.6248 5.616 4.5519 4.4084 4.4161 4.0716 3.8891 4.5464
Actin 0.0401 0.0078 0.0078 0.0119 0.0161 0.0073 0.0321 0.0142 0.0283 0.0287 0.0271 0.0117 0.0094 0.0096 0.0151 0.025 0.0053 0.0112
Bud 0.0011 0.0071 0.002 0.012 0.001 0.0019 0.0012 0.0078 0.0037 0.0009 0.001 0.0005 0.0006 0.006 0.0036 0.0026 0.0004 0.0021
Bud Neck 0.0058 0.0004 0.0008 0.0014 0.0007 0.0015 0.0013 0.0005 0.0007 0.0007 0.001 0.0049 0.0002 0.0007 0.0005 0.0003 0.0006 0.004
Bud Periphery 0.0014 0.0088 0.0037 0.0112 0.0008 0.0023 0.004 0.0195 0.0122 0.0096 0.003 0.0016 0.0039 0.0084 0.0126 0.0172 0.0017 0.0106
Bud Site 0.0243 0.0164 0.0052 0.0032 0.008 0.0006 0.0055 0.0065 0.0066 0.0006 0.0012 0.0005 0.0014 0.0248 0.0043 0.0008 0.0007 0.0004
Cell Periphery 0.0022 0.0033 0.0013 0.0038 0.0003 0.0008 0.0025 0.0035 0.0021 0.0041 0.0011 0.0008 0.0022 0.0027 0.0025 0.0027 0.0019 0.0016
Cytoplasm 0.1352 0.2078 0.2738 0.194 0.1849 0.2279 0.1653 0.2521 0.1651 0.2148 0.1342 0.219 0.1519 0.2137 0.1627 0.0803 0.1777 0.1721
Cytoplasmic Foci 0.0678 0.0246 0.0295 0.0356 0.0345 0.0173 0.0375 0.0121 0.0235 0.014 0.0383 0.0222 0.0186 0.0244 0.0225 0.024 0.0147 0.02
Eisosomes 0.0004 0.0005 0.0006 0.0001 0.0006 0.0001 0.0008 0.0003 0.0004 0.0006 0.0004 0.0002 0.0005 0.0003 0.0005 0.0008 0.0005 0.0003
Endoplasmic Reticulum 0.3737 0.3883 0.3693 0.309 0.3166 0.5515 0.3511 0.3467 0.4038 0.3339 0.3673 0.4327 0.5431 0.4057 0.4911 0.5584 0.4754 0.5254
Endosome 0.089 0.0406 0.0348 0.0629 0.0793 0.0729 0.0661 0.0348 0.0567 0.0493 0.0888 0.0557 0.031 0.04 0.0477 0.0346 0.061 0.0348
Golgi 0.0277 0.01 0.0165 0.0189 0.0604 0.0112 0.0171 0.0086 0.0167 0.0281 0.0469 0.0168 0.0086 0.0142 0.0116 0.0226 0.0115 0.0083
Lipid Particles 0.0289 0.0115 0.0148 0.034 0.0113 0.0075 0.0267 0.0152 0.0253 0.0052 0.0237 0.0121 0.0214 0.0292 0.0193 0.0198 0.0218 0.0139
Mitochondria 0.0167 0.0066 0.0128 0.0427 0.0475 0.0036 0.0111 0.0042 0.0038 0.0029 0.0065 0.003 0.0024 0.0025 0.0059 0.0154 0.0032 0.0028
None 0.0987 0.2314 0.2017 0.2358 0.1947 0.0727 0.2306 0.225 0.2151 0.2705 0.212 0.1956 0.1743 0.1629 0.1532 0.1543 0.1783 0.1588
Nuclear Periphery 0.022 0.0186 0.0085 0.0085 0.0086 0.0097 0.0156 0.0235 0.0169 0.0175 0.021 0.0104 0.0167 0.0258 0.0244 0.0203 0.0305 0.0181
Nucleolus 0.0028 0.0012 0.0004 0.0003 0.0005 0.0003 0.0005 0.0004 0.0004 0.0002 0.0003 0.0002 0.0003 0.0011 0.0004 0.0003 0.0001 0.0003
Nucleus 0.0285 0.0053 0.0023 0.0023 0.0032 0.0028 0.0026 0.0066 0.0033 0.0021 0.005 0.0032 0.0024 0.0101 0.0049 0.0022 0.0035 0.0038
Peroxisomes 0.0099 0.0007 0.0057 0.0026 0.0204 0.0003 0.0086 0.0008 0.0041 0.0005 0.005 0.0009 0.0007 0.0009 0.0009 0.0034 0.0003 0.0007
Punctate Nuclear 0.0097 0.0037 0.0033 0.0023 0.0024 0.0024 0.008 0.0016 0.002 0.002 0.004 0.0017 0.0021 0.0035 0.0023 0.0017 0.002 0.0028
Vacuole 0.011 0.0044 0.0042 0.0054 0.0067 0.004 0.0093 0.0141 0.0073 0.0104 0.0099 0.0049 0.007 0.0119 0.0109 0.0097 0.0063 0.0059
Vacuole Periphery 0.0032 0.0009 0.0008 0.0021 0.0017 0.0014 0.0026 0.0021 0.0022 0.0035 0.0024 0.0014 0.0015 0.0018 0.0031 0.0036 0.0026 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.1563 32.5998 31.102 41.1408 31.0709 28.1166 35.5514 41.6334 38.4877 47.3621
Translational Efficiency 0.8807 0.9552 1.1782 0.771 1.1338 1.3524 1.1256 0.7725 1.0122 0.8357

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein localized to COPII-coated vesicles; forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein
Localization
Cell Percentages ER (75%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Erv41

Erv41


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erv41-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available