Standard name | |
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Human Ortholog | |
Description | Protein localized to COPII-coated vesicles; forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 16 | 8 | 23 | 9 | 15 | 22 | 13 | 21 | – | 19 | 17 | 36 | 35 | 25 | 23 | 9 | 11 | 7 | 15 | 4 | 6 | 15 | 10 | 13 |
Bud | 3 | 0 | 2 | 0 | 9 | 8 | 10 | 15 | – | 16 | 21 | 14 | 8 | 1 | 0 | 2 | 2 | 1 | 2 | 0 | 0 | 2 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 7 | 4 | 18 | 12 | 19 | 38 | – | 23 | 29 | 27 | 8 | 32 | 66 | 18 | 15 | 18 | 1 | 0 | 3 | 1 | 3 | 4 |
Cytoplasm | 44 | 20 | 42 | 35 | 81 | 74 | 113 | 173 | – | 107 | 97 | 95 | 38 | 45 | 53 | 48 | 41 | 45 | 6 | 2 | 7 | 10 | 13 | 11 |
Endoplasmic Reticulum | 264 | 122 | 191 | 93 | 61 | 154 | 119 | 93 | – | 92 | 74 | 165 | 289 | 349 | 396 | 1 | 3 | 0 | 283 | 128 | 221 | 309 | 277 | 289 |
Endosome | 2 | 0 | 5 | 1 | 4 | 5 | 11 | 16 | – | 15 | 2 | 3 | 0 | 1 | 2 | 5 | 0 | 0 | 3 | 1 | 0 | 3 | 1 | 4 |
Golgi | 22 | 2 | 10 | 12 | 12 | 0 | 2 | 3 | – | 0 | 0 | 2 | 7 | 10 | 7 | 6 | 3 | 4 | 6 | 2 | 3 | 10 | 6 | 4 |
Mitochondria | 3 | 1 | 5 | 5 | 18 | 39 | 68 | 96 | – | 105 | 149 | 167 | 0 | 0 | 0 | 14 | 7 | 1 | 3 | 0 | 2 | 7 | 6 | 7 |
Nucleus | 1 | 0 | 0 | 0 | 1 | 1 | 5 | 4 | – | 2 | 3 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 3 | 4 | 2 | 2 | 3 | 4 | 3 | – | 3 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 11 | 4 | 9 | 21 | 88 | 32 | 59 | 117 | – | 68 | 43 | 33 | 9 | 14 | 9 | 31 | 8 | 6 | 3 | 2 | 0 | 11 | 4 | 6 |
Unique Cell Count | 321 | 139 | 241 | 152 | 248 | 290 | 338 | 476 | 370 | 347 | 420 | 342 | 409 | 473 | 108 | 79 | 78 | 331 | 142 | 250 | 383 | 330 | 356 | |
Labelled Cell Count | 368 | 163 | 298 | 182 | 309 | 351 | 425 | 581 | 456 | 439 | 548 | 395 | 479 | 557 | 137 | 93 | 82 | 331 | 142 | 250 | 383 | 330 | 356 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.6 | 18.2 | 13.3 | 12.3 | 12.5 | 9.9 | 9.1 | 9.8 | – | 9.0 | 8.5 | 8.8 | 15.1 | 15.4 | 14.8 | 10.3 | 10.3 | 10.7 | 16.0 | 17.1 | 16.5 |
Std Deviation (1e-4) | 4.1 | 3.2 | 2.8 | 2.4 | 5.8 | 2.7 | 2.5 | 2.7 | – | 2.4 | 2.1 | 2.2 | 2.9 | 2.9 | 2.6 | 2.0 | 2.0 | 2.1 | 3.4 | 3.5 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.09 | -0.42 | -0.54 | -0.44 | – | -0.57 | -0.64 | -0.6 | 0.18 | 0.22 | 0.16 | -0.37 | -0.37 | -0.3 | 0.27 | 0.37 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.3 | -1.4 | -0.8 | -2.8 | -2.7 | – | -2.1 | -2.2 | -0.4 | 0.3 | -1.6 | -2.4 | -0.4 | 1.1 | -0.1 |
Bud | 0 | 2.1 | 0 | 0 | 1.9 | – | 2.5 | 3.2 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 2.2 | 0.8 | 1.6 | 2.6 | – | 1.9 | 2.7 | 2.0 | -0.4 | 2.6 | 4.6 | 4.6 | 4.9 | 5.8 |
Cytoplasm | 1.4 | 3.9 | 2.2 | 4.3 | 5.2 | – | 3.2 | 3.0 | 1.6 | -2.2 | -2.3 | -2.3 | 5.3 | 6.1 | 6.9 |
Endoplasmic Reticulum | -3.9 | -12.1 | -6.3 | -10.5 | -15.4 | – | -13.2 | -13.9 | -9.9 | 1.6 | 2.0 | 1.5 | -13.6 | -11.9 | -12.4 |
Endosome | 0 | 0 | 0 | 0.9 | 1.0 | – | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.6 | 0.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.5 | -1.2 | -2.2 | 0 | 0 | 0 |
Mitochondria | 0 | 2.7 | 4.7 | 6.4 | 6.6 | – | 8.3 | 11.1 | 10.6 | 0 | 0 | 0 | 4.1 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.7 | 8.8 | 3.1 | 5.1 | 6.9 | – | 5.3 | 3.6 | 2.1 | -0.8 | -0.2 | -1.5 | 6.8 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9128 | 3.9231 | 2.7877 | 2.8534 | 2.4455 | 4.486 | 5.5041 | 5.4463 | 5.8215 | 4.9703 | 5.6248 | 5.616 | 4.5519 | 4.4084 | 4.4161 | 4.0716 | 3.8891 | 4.5464 |
Actin | 0.0401 | 0.0078 | 0.0078 | 0.0119 | 0.0161 | 0.0073 | 0.0321 | 0.0142 | 0.0283 | 0.0287 | 0.0271 | 0.0117 | 0.0094 | 0.0096 | 0.0151 | 0.025 | 0.0053 | 0.0112 |
Bud | 0.0011 | 0.0071 | 0.002 | 0.012 | 0.001 | 0.0019 | 0.0012 | 0.0078 | 0.0037 | 0.0009 | 0.001 | 0.0005 | 0.0006 | 0.006 | 0.0036 | 0.0026 | 0.0004 | 0.0021 |
Bud Neck | 0.0058 | 0.0004 | 0.0008 | 0.0014 | 0.0007 | 0.0015 | 0.0013 | 0.0005 | 0.0007 | 0.0007 | 0.001 | 0.0049 | 0.0002 | 0.0007 | 0.0005 | 0.0003 | 0.0006 | 0.004 |
Bud Periphery | 0.0014 | 0.0088 | 0.0037 | 0.0112 | 0.0008 | 0.0023 | 0.004 | 0.0195 | 0.0122 | 0.0096 | 0.003 | 0.0016 | 0.0039 | 0.0084 | 0.0126 | 0.0172 | 0.0017 | 0.0106 |
Bud Site | 0.0243 | 0.0164 | 0.0052 | 0.0032 | 0.008 | 0.0006 | 0.0055 | 0.0065 | 0.0066 | 0.0006 | 0.0012 | 0.0005 | 0.0014 | 0.0248 | 0.0043 | 0.0008 | 0.0007 | 0.0004 |
Cell Periphery | 0.0022 | 0.0033 | 0.0013 | 0.0038 | 0.0003 | 0.0008 | 0.0025 | 0.0035 | 0.0021 | 0.0041 | 0.0011 | 0.0008 | 0.0022 | 0.0027 | 0.0025 | 0.0027 | 0.0019 | 0.0016 |
Cytoplasm | 0.1352 | 0.2078 | 0.2738 | 0.194 | 0.1849 | 0.2279 | 0.1653 | 0.2521 | 0.1651 | 0.2148 | 0.1342 | 0.219 | 0.1519 | 0.2137 | 0.1627 | 0.0803 | 0.1777 | 0.1721 |
Cytoplasmic Foci | 0.0678 | 0.0246 | 0.0295 | 0.0356 | 0.0345 | 0.0173 | 0.0375 | 0.0121 | 0.0235 | 0.014 | 0.0383 | 0.0222 | 0.0186 | 0.0244 | 0.0225 | 0.024 | 0.0147 | 0.02 |
Eisosomes | 0.0004 | 0.0005 | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0.0008 | 0.0003 | 0.0004 | 0.0006 | 0.0004 | 0.0002 | 0.0005 | 0.0003 | 0.0005 | 0.0008 | 0.0005 | 0.0003 |
Endoplasmic Reticulum | 0.3737 | 0.3883 | 0.3693 | 0.309 | 0.3166 | 0.5515 | 0.3511 | 0.3467 | 0.4038 | 0.3339 | 0.3673 | 0.4327 | 0.5431 | 0.4057 | 0.4911 | 0.5584 | 0.4754 | 0.5254 |
Endosome | 0.089 | 0.0406 | 0.0348 | 0.0629 | 0.0793 | 0.0729 | 0.0661 | 0.0348 | 0.0567 | 0.0493 | 0.0888 | 0.0557 | 0.031 | 0.04 | 0.0477 | 0.0346 | 0.061 | 0.0348 |
Golgi | 0.0277 | 0.01 | 0.0165 | 0.0189 | 0.0604 | 0.0112 | 0.0171 | 0.0086 | 0.0167 | 0.0281 | 0.0469 | 0.0168 | 0.0086 | 0.0142 | 0.0116 | 0.0226 | 0.0115 | 0.0083 |
Lipid Particles | 0.0289 | 0.0115 | 0.0148 | 0.034 | 0.0113 | 0.0075 | 0.0267 | 0.0152 | 0.0253 | 0.0052 | 0.0237 | 0.0121 | 0.0214 | 0.0292 | 0.0193 | 0.0198 | 0.0218 | 0.0139 |
Mitochondria | 0.0167 | 0.0066 | 0.0128 | 0.0427 | 0.0475 | 0.0036 | 0.0111 | 0.0042 | 0.0038 | 0.0029 | 0.0065 | 0.003 | 0.0024 | 0.0025 | 0.0059 | 0.0154 | 0.0032 | 0.0028 |
None | 0.0987 | 0.2314 | 0.2017 | 0.2358 | 0.1947 | 0.0727 | 0.2306 | 0.225 | 0.2151 | 0.2705 | 0.212 | 0.1956 | 0.1743 | 0.1629 | 0.1532 | 0.1543 | 0.1783 | 0.1588 |
Nuclear Periphery | 0.022 | 0.0186 | 0.0085 | 0.0085 | 0.0086 | 0.0097 | 0.0156 | 0.0235 | 0.0169 | 0.0175 | 0.021 | 0.0104 | 0.0167 | 0.0258 | 0.0244 | 0.0203 | 0.0305 | 0.0181 |
Nucleolus | 0.0028 | 0.0012 | 0.0004 | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0011 | 0.0004 | 0.0003 | 0.0001 | 0.0003 |
Nucleus | 0.0285 | 0.0053 | 0.0023 | 0.0023 | 0.0032 | 0.0028 | 0.0026 | 0.0066 | 0.0033 | 0.0021 | 0.005 | 0.0032 | 0.0024 | 0.0101 | 0.0049 | 0.0022 | 0.0035 | 0.0038 |
Peroxisomes | 0.0099 | 0.0007 | 0.0057 | 0.0026 | 0.0204 | 0.0003 | 0.0086 | 0.0008 | 0.0041 | 0.0005 | 0.005 | 0.0009 | 0.0007 | 0.0009 | 0.0009 | 0.0034 | 0.0003 | 0.0007 |
Punctate Nuclear | 0.0097 | 0.0037 | 0.0033 | 0.0023 | 0.0024 | 0.0024 | 0.008 | 0.0016 | 0.002 | 0.002 | 0.004 | 0.0017 | 0.0021 | 0.0035 | 0.0023 | 0.0017 | 0.002 | 0.0028 |
Vacuole | 0.011 | 0.0044 | 0.0042 | 0.0054 | 0.0067 | 0.004 | 0.0093 | 0.0141 | 0.0073 | 0.0104 | 0.0099 | 0.0049 | 0.007 | 0.0119 | 0.0109 | 0.0097 | 0.0063 | 0.0059 |
Vacuole Periphery | 0.0032 | 0.0009 | 0.0008 | 0.0021 | 0.0017 | 0.0014 | 0.0026 | 0.0021 | 0.0022 | 0.0035 | 0.0024 | 0.0014 | 0.0015 | 0.0018 | 0.0031 | 0.0036 | 0.0026 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.1563 | 32.5998 | 31.102 | 41.1408 | 31.0709 | 28.1166 | 35.5514 | 41.6334 | 38.4877 | 47.3621 |
Translational Efficiency | 0.8807 | 0.9552 | 1.1782 | 0.771 | 1.1338 | 1.3524 | 1.1256 | 0.7725 | 1.0122 | 0.8357 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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