Standard name | |
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Human Ortholog | |
Description | HMG-CoA reductase; catalyzes conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to nuclear envelope; overproduction induces formation of karmellae; forms foci at nuclear periphery upon DNA replication stress; HMG1 has a paralog, HMG2, that arose from the whole genome duplication; human homolog HMGCR can complement yeast hmg1 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 7 | 6 | 2 | 0 | 0 | 0 | 1 | 1 | 4 | 1 | 2 | 1 |
Bud | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 3 | – | 3 | 1 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 4 | 1 | 1 | 4 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 3 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 4 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 10 | 6 | 9 | 4 | 21 | 11 | 18 | 13 | – | 4 | 6 | 4 | 6 | 5 | 17 | 8 | 7 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 10 | 3 | 5 | 4 | 2 | 1 | 2 | 1 | – | 0 | 1 | 0 | 12 | 2 | 3 | 1 | 3 | 2 | 16 | 18 | 54 | 16 | 29 | 15 |
Endosome | 1 | 0 | 1 | 1 | 4 | 1 | 2 | 6 | – | 0 | 0 | 5 | 8 | 1 | 1 | 2 | 1 | 1 | 2 | 2 | 3 | 1 | 5 | 11 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 2 | 1 |
Mitochondria | 0 | 8 | 12 | 9 | 18 | 24 | 31 | 35 | – | 84 | 72 | 33 | 0 | 0 | 1 | 4 | 2 | 1 | 4 | 5 | 3 | 3 | 11 | 11 |
Nucleus | 24 | 20 | 6 | 2 | 5 | 35 | 40 | 70 | – | 112 | 104 | 86 | 0 | 8 | 12 | 6 | 6 | 0 | 1 | 10 | 1 | 2 | 3 | 2 |
Nuclear Periphery | 316 | 123 | 238 | 125 | 152 | 186 | 231 | 284 | – | 170 | 151 | 177 | 90 | 53 | 63 | 27 | 14 | 6 | 426 | 191 | 236 | 96 | 198 | 152 |
Nucleolus | 10 | 6 | 15 | 7 | 26 | 44 | 74 | 107 | – | 79 | 73 | 89 | 15 | 2 | 17 | 3 | 2 | 2 | 1 | 0 | 1 | 1 | 3 | 6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | – | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 2 | 1 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 195 | 109 | 80 | 52 | 76 | 69 | 54 | 80 | – | 45 | 23 | 52 | 50 | 31 | 48 | 23 | 14 | 11 | 20 | 37 | 11 | 14 | 52 | 67 |
Unique Cell Count | 451 | 237 | 298 | 171 | 245 | 303 | 364 | 506 | 400 | 346 | 364 | 152 | 95 | 142 | 57 | 37 | 20 | 481 | 283 | 325 | 143 | 318 | 281 | |
Labelled Cell Count | 569 | 276 | 367 | 205 | 306 | 373 | 459 | 602 | 500 | 438 | 452 | 195 | 115 | 178 | 75 | 49 | 23 | 481 | 283 | 325 | 143 | 318 | 281 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.4 | 13.0 | 13.7 | 14.6 | 13.6 | 12.1 | 11.6 | 11.4 | – | 9.1 | 9.7 | 10.8 | 14.4 | 14.8 | 15.2 | 8.8 | 8.1 | 12.0 | 14.9 | 16.6 | 16.3 |
Std Deviation (1e-4) | 4.5 | 3.5 | 3.3 | 3.6 | 3.4 | 2.7 | 3.3 | 3.0 | – | 3.3 | 3.6 | 3.1 | 3.1 | 3.2 | 3.9 | 2.8 | 2.4 | 3.5 | 4.0 | 4.5 | 3.9 |
Intensity Change (Log2) | – | – | – | 0.1 | -0.0 | -0.18 | -0.24 | -0.26 | – | -0.59 | -0.49 | -0.34 | 0.08 | 0.12 | 0.16 | -0.64 | -0.75 | -0.18 | 0.13 | 0.29 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.8 | 0.4 | 1.2 | -0.4 | – | -2.0 | -1.1 | -1.8 | 0.5 | 0 | 3.7 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.6 | 1.7 | 2.0 | 2.3 | 1.7 | – | 6.4 | 6.3 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 4.6 | 4.5 | 5.5 | – | 9.1 | 9.4 | 8.0 | 0 | 0 | 3.2 | 0 | 0 | 0 |
Nuclear Periphery | -1.7 | -4.6 | -5.0 | -4.6 | -6.8 | – | -9.9 | -9.4 | -8.3 | -4.7 | -4.7 | -7.5 | -5.2 | -5.6 | -5.1 |
Nucleolus | -0.5 | 2.4 | 3.9 | 5.7 | 6.2 | – | 5.6 | 5.9 | 6.8 | 1.9 | 0 | 2.6 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.8 | 1.1 | -1.2 | -3.8 | -3.8 | – | -5.3 | -7.0 | -4.0 | 1.3 | 1.1 | 1.5 | 2.1 | 1.4 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.6578 | 12.1428 | 13.0989 | 10.8182 | 11.8708 | 12.2794 | 14.4153 | 17.5498 | 15.7674 | 16.8519 | 14.3247 | 15.1691 | 13.4747 | 17.6308 | 15.298 | 15.7169 | 14.2493 | 14.8318 |
Actin | 0.0144 | 0.0002 | 0.0168 | 0.0059 | 0.0062 | 0.0006 | 0.0235 | 0.0005 | 0.0005 | 0.0038 | 0.0087 | 0.0051 | 0.0027 | 0.0002 | 0.0205 | 0.0006 | 0.0011 | 0.0002 |
Bud | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0008 | 0 | 0.0006 | 0 |
Bud Neck | 0.0024 | 0.0002 | 0.0042 | 0.0004 | 0.001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 |
Bud Periphery | 0.0002 | 0.0001 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0011 | 0.0004 | 0.0019 | 0 |
Bud Site | 0.0008 | 0.0001 | 0.001 | 0.0003 | 0.0001 | 0 | 0.0007 | 0 | 0 | 0.0018 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0036 | 0 | 0.0012 | 0 |
Cell Periphery | 0.0002 | 0.0003 | 0.0006 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0008 | 0.0001 | 0.0001 | 0.0009 | 0.0017 | 0.0008 | 0.0003 | 0.0007 | 0 |
Cytoplasm | 0.0037 | 0.01 | 0.0017 | 0.0027 | 0.002 | 0.0005 | 0.003 | 0.0005 | 0.0007 | 0.0003 | 0.0005 | 0.0001 | 0.0224 | 0.0003 | 0.0109 | 0.0002 | 0.0009 | 0.0003 |
Cytoplasmic Foci | 0.0014 | 0.0002 | 0.0008 | 0.0028 | 0.0005 | 0.0003 | 0.0118 | 0 | 0.0045 | 0.0011 | 0.0078 | 0.0012 | 0.009 | 0 | 0.0039 | 0 | 0.0014 | 0.0001 |
Eisosomes | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0087 | 0.0396 | 0.0517 | 0.0858 | 0.0486 | 0.0092 | 0.042 | 0.2841 | 0.2353 | 0.254 | 0.1143 | 0.0677 | 0.056 | 0.403 | 0.3075 | 0.2678 | 0.5308 | 0.0402 |
Endosome | 0.0063 | 0.0025 | 0.0047 | 0.0077 | 0.0125 | 0.0016 | 0.0146 | 0.001 | 0.0029 | 0.0032 | 0.0056 | 0.0017 | 0.0488 | 0.001 | 0.0183 | 0.001 | 0.0353 | 0.0002 |
Golgi | 0.0017 | 0 | 0.002 | 0.0043 | 0.002 | 0.0002 | 0.0044 | 0.0001 | 0.0008 | 0.0031 | 0.0055 | 0.0038 | 0.0023 | 0.0002 | 0.0195 | 0.0002 | 0.0048 | 0.0001 |
Lipid Particles | 0.0017 | 0.0002 | 0.0017 | 0.0194 | 0.0012 | 0.0002 | 0.0162 | 0.0004 | 0.0125 | 0.0064 | 0.0088 | 0.0003 | 0.0356 | 0.0005 | 0.0018 | 0.0003 | 0.0062 | 0.0002 |
Mitochondria | 0.0026 | 0.0003 | 0.0022 | 0.0055 | 0.003 | 0.0004 | 0.0013 | 0.0008 | 0.0011 | 0.0398 | 0.0017 | 0.002 | 0.0022 | 0.0008 | 0.0078 | 0.001 | 0.0237 | 0.0003 |
None | 0.001 | 0.0001 | 0.0009 | 0.0007 | 0.0006 | 0.0001 | 0.0013 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.1248 | 0.0001 | 0.0008 | 0.0001 | 0.0014 | 0.0233 |
Nuclear Periphery | 0.8308 | 0.7389 | 0.7824 | 0.7686 | 0.8089 | 0.9205 | 0.7777 | 0.6399 | 0.6633 | 0.6142 | 0.7702 | 0.8815 | 0.6146 | 0.5225 | 0.5325 | 0.6717 | 0.3355 | 0.8896 |
Nucleolus | 0.0008 | 0.0009 | 0.001 | 0.0007 | 0.0015 | 0.0003 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0012 | 0 | 0.0001 | 0 | 0.0009 | 0.0019 |
Nucleus | 0.0909 | 0.1638 | 0.082 | 0.0271 | 0.0691 | 0.0373 | 0.0394 | 0.0077 | 0.0061 | 0.0122 | 0.0146 | 0.0027 | 0.0124 | 0.0101 | 0.0067 | 0.004 | 0.0037 | 0.0041 |
Peroxisomes | 0.0003 | 0 | 0.0003 | 0.004 | 0.0001 | 0.0001 | 0.0011 | 0 | 0.0001 | 0.0052 | 0.0051 | 0.001 | 0.0007 | 0 | 0.001 | 0 | 0.0007 | 0 |
Punctate Nuclear | 0.0032 | 0.0004 | 0.0013 | 0.0015 | 0.0016 | 0.0012 | 0.0137 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0177 | 0.0001 | 0.0014 | 0.0001 | 0.0004 | 0.0032 |
Vacuole | 0.0017 | 0.0174 | 0.0057 | 0.0257 | 0.0075 | 0.0066 | 0.0024 | 0.0088 | 0.0082 | 0.0062 | 0.0041 | 0.0018 | 0.0062 | 0.0082 | 0.0071 | 0.0041 | 0.0149 | 0.0006 |
Vacuole Periphery | 0.0272 | 0.0248 | 0.0376 | 0.0356 | 0.0331 | 0.0205 | 0.0451 | 0.0553 | 0.062 | 0.0461 | 0.0523 | 0.03 | 0.0402 | 0.0511 | 0.0536 | 0.048 | 0.0338 | 0.0353 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 39.9178 | 38.3331 | 43.4766 | 51.5701 | 37.7428 | 33.7981 | 43.4064 | 50.1921 | 47.9035 | 48.0421 |
Translational Efficiency | 0.6508 | 0.6644 | 0.7976 | 0.6477 | 0.5517 | 0.7021 | 0.6129 | 0.4528 | 0.5763 | 0.5187 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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