Standard name | |
---|---|
Human Ortholog | |
Description | Protein required for nuclear retention of unspliced pre-mRNAs; required along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 2 | 2 | 3 |
Bud | 0 | 0 | 1 | 3 | 0 | 8 | 26 | 7 | 17 | 17 | 18 | 19 | 0 | 0 | 0 | 1 | 4 | 0 | 1 | 1 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 2 |
Bud Site | 1 | 0 | 0 | 1 | 2 | 8 | 11 | 11 | 10 | 15 | 15 | 22 | 0 | 0 | 0 | 1 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 4 | 8 | 14 | 16 | 10 | 15 | 20 | 12 | 8 | 7 | 17 | 41 | 66 | 89 | 27 | 24 | 15 | 0 | 0 | 2 | 3 | 9 | 5 |
Endoplasmic Reticulum | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 12 | 10 | 9 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 8 | 1 | 102 | 34 | 73 | 14 | 19 | 34 | 11 | 0 | 4 | 5 | 106 | 145 | 192 | 99 | 109 | 74 | 40 | 10 | 59 | 53 | 68 | 107 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 1 | 4 |
Mitochondria | 179 | 53 | 127 | 142 | 156 | 321 | 421 | 336 | 313 | 383 | 349 | 397 | 64 | 24 | 42 | 37 | 29 | 21 | 12 | 4 | 20 | 34 | 38 | 57 |
Nucleus | 15 | 2 | 4 | 9 | 18 | 35 | 28 | 24 | 46 | 52 | 40 | 46 | 19 | 18 | 22 | 20 | 4 | 10 | 8 | 2 | 0 | 18 | 15 | 14 |
Nuclear Periphery | 116 | 33 | 78 | 58 | 39 | 30 | 32 | 44 | 38 | 47 | 40 | 57 | 68 | 67 | 25 | 62 | 66 | 46 | 175 | 57 | 193 | 166 | 191 | 256 |
Nucleolus | 19 | 17 | 17 | 11 | 7 | 14 | 26 | 30 | 23 | 28 | 27 | 24 | 11 | 1 | 3 | 19 | 16 | 17 | 11 | 4 | 4 | 18 | 17 | 16 |
Peroxisomes | 0 | 1 | 17 | 7 | 12 | 4 | 9 | 19 | 5 | 3 | 2 | 4 | 6 | 19 | 35 | 7 | 0 | 3 | 3 | 0 | 5 | 4 | 5 | 8 |
SpindlePole | 2 | 0 | 12 | 15 | 8 | 15 | 30 | 26 | 22 | 15 | 9 | 21 | 21 | 23 | 38 | 21 | 13 | 10 | 7 | 6 | 17 | 28 | 44 | 29 |
Vac/Vac Membrane | 48 | 19 | 46 | 12 | 34 | 9 | 27 | 20 | 12 | 16 | 15 | 22 | 49 | 101 | 94 | 81 | 54 | 62 | 13 | 2 | 4 | 11 | 15 | 16 |
Unique Cell Count | 249 | 85 | 277 | 220 | 287 | 368 | 493 | 451 | 377 | 433 | 390 | 464 | 307 | 371 | 428 | 255 | 216 | 175 | 284 | 93 | 325 | 359 | 429 | 542 |
Labelled Cell Count | 395 | 131 | 412 | 306 | 367 | 468 | 646 | 572 | 510 | 585 | 526 | 635 | 388 | 465 | 542 | 387 | 332 | 267 | 284 | 93 | 325 | 359 | 429 | 542 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 5.0 | 5.2 | 4.9 | 4.8 | 3.7 | 3.8 | 4.1 | 3.9 | 3.5 | 3.3 | 3.4 | 6.1 | 6.1 | 5.9 | 6.4 | 6.4 | 6.3 | 6.0 | 6.1 | 6.8 |
Std Deviation (1e-4) | 0.6 | 0.8 | 1.8 | 2.7 | 2.4 | 1.2 | 1.1 | 1.6 | 1.9 | 0.7 | 1.0 | 1.3 | 1.7 | 1.7 | 1.8 | 1.3 | 1.6 | 1.4 | 1.2 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.11 | -0.48 | -0.46 | -0.36 | -0.42 | -0.6 | -0.65 | -0.61 | 0.21 | 0.22 | 0.18 | 0.28 | 0.29 | 0.27 | 0.2 | 0.22 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.9 | 1.6 | -0.1 | 0.1 | 1.1 | 0.2 | -0.9 | -0.9 | 0.6 | 4.6 | 5.9 | 6.7 | 3.6 | 3.7 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | 0.6 | 2.1 | 0.5 | 3.0 | 1.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.4 | -11.7 | -10.9 | -7.8 | -7.7 | -7.5 |
Nucleus | 1.8 | 3.0 | 4.3 | 2.8 | 2.6 | 5.1 | 5.1 | 4.5 | 4.4 | 2.9 | 2.4 | 2.5 | 3.6 | 0 | 2.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.7 | -3.1 | -8.2 | -1.0 | 0.6 | -0.4 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.4 | -4.5 | -4.2 | 0.6 | 0.6 | 1.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.6 | -0.6 | 1.0 | -1.9 | -3.7 | -2.2 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 1.0 | 2.3 | 1.9 | 0.8 | 0.7 |
Vacuole | -3.8 | -1.6 | -6.4 | -5.1 | -5.6 | -6.0 | -5.9 | -5.6 | -5.4 | -0.2 | 3.2 | 1.7 | 4.1 | 2.3 | 4.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.4553 | 0.5268 | 0.4469 | 0.6363 | -0.0488 | 0.4538 | 0.8557 | 0.6528 | 0.538 | 0.1163 | 0.6672 | 0.3992 | -0.284 | -0.4164 | -0.1807 | -0.2523 | 0.1111 | -0.3169 |
Actin | 0.0067 | 0.0003 | 0.0008 | 0.0028 | 0.0455 | 0.0005 | 0.0135 | 0.0009 | 0.005 | 0.0163 | 0.01 | 0.0015 | 0.0244 | 0.0005 | 0.0066 | 0.0037 | 0.0066 | 0.0006 |
Bud | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 |
Bud Neck | 0.0007 | 0.0001 | 0.0001 | 0.0011 | 0.0003 | 0.0002 | 0.0012 | 0.0002 | 0.0002 | 0.0004 | 0.0019 | 0.0008 | 0.0006 | 0.0001 | 0.001 | 0.0002 | 0.0022 | 0.0004 |
Bud Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0006 | 0.0004 | 0.0006 | 0.0002 | 0.0007 | 0.0001 | 0.0003 | 0.0002 | 0.0013 | 0.0001 |
Bud Site | 0.0011 | 0 | 0 | 0 | 0.0002 | 0 | 0.0019 | 0.0003 | 0.0004 | 0.0004 | 0.0014 | 0.0001 | 0.0035 | 0.0001 | 0.0033 | 0.0002 | 0.0087 | 0 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 |
Cytoplasm | 0.0122 | 0.0015 | 0.0017 | 0.0085 | 0.0021 | 0.0067 | 0.0066 | 0.0041 | 0.0026 | 0.0014 | 0.0089 | 0.0009 | 0.0011 | 0.0016 | 0.0041 | 0.0032 | 0.0042 | 0.0006 |
Cytoplasmic Foci | 0.0031 | 0.0007 | 0.0013 | 0.0014 | 0.0054 | 0.0016 | 0.0193 | 0.0025 | 0.0048 | 0.0045 | 0.0107 | 0.0014 | 0.01 | 0.0022 | 0.0149 | 0.0038 | 0.0186 | 0.0021 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.002 | 0.0051 | 0.0021 | 0.0043 | 0.0008 | 0.0009 | 0.0045 | 0.0051 | 0.0046 | 0.0028 | 0.008 | 0.0017 | 0.0028 | 0.0037 | 0.0027 | 0.0047 | 0.0035 | 0.0007 |
Endosome | 0.007 | 0.0047 | 0.0036 | 0.006 | 0.0077 | 0.0034 | 0.0328 | 0.0143 | 0.0102 | 0.012 | 0.0635 | 0.0062 | 0.03 | 0.011 | 0.0253 | 0.0099 | 0.0262 | 0.0063 |
Golgi | 0.0027 | 0.0006 | 0.0008 | 0.0005 | 0.0047 | 0.0007 | 0.0067 | 0.002 | 0.0052 | 0.0057 | 0.0168 | 0.0034 | 0.018 | 0.0016 | 0.0153 | 0.003 | 0.015 | 0.0037 |
Lipid Particles | 0.0338 | 0.0181 | 0.0179 | 0.0051 | 0.0803 | 0.0258 | 0.0467 | 0.0285 | 0.0262 | 0.0289 | 0.0541 | 0.0256 | 0.0829 | 0.0359 | 0.0441 | 0.063 | 0.0538 | 0.0319 |
Mitochondria | 0.0024 | 0.0009 | 0.0015 | 0.0014 | 0.0059 | 0.0009 | 0.0024 | 0.001 | 0.0083 | 0.008 | 0.0082 | 0.0029 | 0.0181 | 0.0007 | 0.0092 | 0.0055 | 0.0172 | 0.0013 |
None | 0.0207 | 0.0132 | 0.0193 | 0.0688 | 0.0108 | 0.019 | 0.0047 | 0.0017 | 0.0048 | 0.0027 | 0.0195 | 0.0013 | 0.0028 | 0.0025 | 0.0022 | 0.0033 | 0.0572 | 0.0005 |
Nuclear Periphery | 0.8266 | 0.8705 | 0.859 | 0.7099 | 0.7499 | 0.8394 | 0.6849 | 0.7743 | 0.8031 | 0.7687 | 0.6844 | 0.7907 | 0.6995 | 0.8197 | 0.7698 | 0.7322 | 0.6692 | 0.8294 |
Nucleolus | 0.0078 | 0.0118 | 0.0065 | 0.0233 | 0.0027 | 0.0098 | 0.0078 | 0.014 | 0.0086 | 0.0055 | 0.0091 | 0.0306 | 0.0102 | 0.0126 | 0.0125 | 0.0043 | 0.03 | 0.0124 |
Nucleus | 0.0285 | 0.0324 | 0.0178 | 0.0484 | 0.019 | 0.0359 | 0.0283 | 0.0654 | 0.0474 | 0.0363 | 0.0511 | 0.06 | 0.0188 | 0.0595 | 0.0282 | 0.0821 | 0.0126 | 0.0437 |
Peroxisomes | 0.003 | 0.0002 | 0.0005 | 0.0005 | 0.011 | 0.0006 | 0.0072 | 0.001 | 0.0062 | 0.0045 | 0.0055 | 0.0008 | 0.0089 | 0.0005 | 0.0068 | 0.0045 | 0.0061 | 0.0006 |
Punctate Nuclear | 0.0307 | 0.0247 | 0.0523 | 0.1061 | 0.0438 | 0.0476 | 0.1186 | 0.0689 | 0.0487 | 0.0897 | 0.0295 | 0.0635 | 0.0472 | 0.0324 | 0.0405 | 0.0596 | 0.0518 | 0.059 |
Vacuole | 0.0009 | 0.0012 | 0.0007 | 0.0012 | 0.0006 | 0.0006 | 0.0033 | 0.0038 | 0.0011 | 0.0007 | 0.0029 | 0.001 | 0.0059 | 0.0021 | 0.0015 | 0.0011 | 0.0036 | 0.0006 |
Vacuole Periphery | 0.0097 | 0.0136 | 0.0141 | 0.0103 | 0.0086 | 0.0062 | 0.0089 | 0.0117 | 0.0113 | 0.0106 | 0.0131 | 0.0073 | 0.0138 | 0.0132 | 0.0113 | 0.0153 | 0.0109 | 0.006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.1364 | 7.8518 | 10.9244 | 10.5049 | 14.9467 | 6.5838 | 12.4045 | 7.0327 | 10.4886 | 8.4054 |
Translational Efficiency | 1.0495 | 0.8889 | 0.7663 | 0.7095 | 0.4454 | 1.1868 | 0.9443 | 1.4538 | 0.9636 | 1.1793 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1280 | 410 | 1589 | 1384 | 1612 | 358 | 282 | 1419 | 2892 | 768 | 1871 | 2803 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 724.74 | 861.87 | 933.03 | 830.73 | 741.23 | 797.32 | 975.11 | 900.35 | 733.93 | 831.78 | 939.37 | 865.97 |
Standard Deviation | 91.51 | 113.15 | 114.70 | 126.11 | 91.81 | 98.23 | 105.42 | 123.36 | 92.04 | 111.22 | 114.35 | 129.49 |
Intensity Change Log 2 | — | 0.250007 | 0.364460 | 0.196916 | — | 0.105238 | 0.395644 | 0.280565 | — | 0.178624 | 0.380311 | 0.239817 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000144 | 0.000155 | 0.000550 | 0.000523 | 0.000100 | 0.000796 | 0.000843 | 0.000299 | 0.000120 | 0.000454 | 0.000594 | 0.000410 |
Bud Neck | 0.000722 | 0.000460 | 0.002425 | 0.002209 | 0.000369 | 0.000399 | 0.001073 | 0.001012 | 0.000525 | 0.000432 | 0.002221 | 0.001603 |
Bud Site | 0.000136 | 0.000393 | 0.000478 | 0.002949 | 0.000078 | 0.000268 | 0.000291 | 0.001561 | 0.000103 | 0.000335 | 0.000450 | 0.002246 |
Cell Periphery | 0.000043 | 0.000025 | 0.000032 | 0.000058 | 0.000038 | 0.000045 | 0.000052 | 0.000022 | 0.000040 | 0.000034 | 0.000035 | 0.000040 |
Cytoplasm | 0.006983 | 0.002550 | 0.003831 | 0.009915 | 0.012961 | 0.005896 | 0.006574 | 0.005253 | 0.010315 | 0.004110 | 0.004244 | 0.007555 |
Cytoplasmic Foci | 0.006099 | 0.003914 | 0.000419 | 0.002023 | 0.005149 | 0.007713 | 0.001803 | 0.003985 | 0.005569 | 0.005685 | 0.000627 | 0.003017 |
Eisosomes | 0.000020 | 0.000009 | 0.000014 | 0.000016 | 0.000017 | 0.000027 | 0.000044 | 0.000008 | 0.000018 | 0.000017 | 0.000018 | 0.000012 |
Endoplasmic Reticulum | 0.005257 | 0.004977 | 0.005738 | 0.008451 | 0.004310 | 0.004280 | 0.010548 | 0.004501 | 0.004729 | 0.004652 | 0.006463 | 0.006452 |
Endosome | 0.004569 | 0.003781 | 0.001428 | 0.005499 | 0.003036 | 0.006703 | 0.002187 | 0.005372 | 0.003715 | 0.005143 | 0.001542 | 0.005435 |
Golgi | 0.001435 | 0.000859 | 0.000148 | 0.000701 | 0.001047 | 0.003989 | 0.000179 | 0.002228 | 0.001219 | 0.002318 | 0.000153 | 0.001474 |
Lipid Particles | 0.104844* | 0.045989 | 0.001126 | 0.005803 | 0.057305 | 0.061227 | 0.009010 | 0.006854 | 0.078346 | 0.053092 | 0.002314 | 0.006335 |
Mitochondria | 0.008159 | 0.005286 | 0.002653 | 0.003484 | 0.005835 | 0.010869 | 0.002328 | 0.001733 | 0.006864 | 0.007888 | 0.002604 | 0.002598 |
Mitotic Spindle | 0.016361 | 0.051683 | 0.125371* | 0.189009* | 0.035954 | 0.042998 | 0.076311 | 0.272237* | 0.027282 | 0.047635 | 0.117976* | 0.231142* |
None | 0.004828 | 0.000478 | 0.001111 | 0.001650 | 0.006645 | 0.001739 | 0.002622 | 0.000536 | 0.005841 | 0.001066 | 0.001339 | 0.001086 |
Nuclear Periphery | 0.072838 | 0.070867 | 0.182843* | 0.134962* | 0.071763 | 0.096496 | 0.137484* | 0.125236* | 0.072239 | 0.082814 | 0.176006* | 0.130038* |
Nuclear Periphery Foci | 0.713125* | 0.787265* | 0.496975* | 0.572786* | 0.725796* | 0.669700* | 0.558774* | 0.543158* | 0.720188* | 0.732462* | 0.506290* | 0.557787* |
Nucleolus | 0.027861 | 0.008377 | 0.073801 | 0.015016 | 0.038024 | 0.036539 | 0.059600 | 0.006563 | 0.033526 | 0.021505 | 0.071660 | 0.010737 |
Nucleus | 0.023843 | 0.010496 | 0.098136 | 0.034810 | 0.030029 | 0.041813 | 0.124625 | 0.011094 | 0.027292 | 0.025094 | 0.102128 | 0.022804 |
Peroxisomes | 0.001445 | 0.000706 | 0.000344 | 0.000896 | 0.000694 | 0.002176 | 0.001107 | 0.000576 | 0.001026 | 0.001391 | 0.000459 | 0.000734 |
Vacuole | 0.000776 | 0.000830 | 0.002088 | 0.007342 | 0.000511 | 0.002990 | 0.004272 | 0.005978 | 0.000628 | 0.001836 | 0.002417 | 0.006651 |
Vacuole Periphery | 0.000510 | 0.000900 | 0.000490 | 0.001897 | 0.000339 | 0.003338 | 0.000273 | 0.001794 | 0.000415 | 0.002037 | 0.000457 | 0.001845 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.04 | -11.48 | -3.60 | -3.50 | 0.86 | -1.30 | -7.61 | -3.75 | 0.74 | 5.50 | -1.31 | -14.63 | -5.11 | -0.14 | 3.83 |
Bud Neck | 0.60 | -4.88 | -4.08 | -6.08 | 1.46 | -0.74 | -6.11 | -7.71 | -6.83 | 1.48 | 0.40 | -7.70 | -6.84 | -8.09 | 3.09 |
Bud Site | -0.84 | -4.67 | -3.51 | -3.05 | -2.83 | -1.65 | -4.07 | -2.72 | -2.39 | -1.14 | -1.39 | -6.22 | -4.44 | -3.85 | -3.19 |
Cell Periphery | 3.38 | 1.40 | -0.99 | -2.21 | -1.43 | -0.80 | -5.12 | 3.98 | 2.44 | 6.81 | 1.36 | -0.85 | -0.12 | -0.88 | 0.23 |
Cytoplasm | 2.34 | 1.81 | -2.40 | -4.20 | -3.97 | 2.96 | 2.13 | 2.87 | -0.79 | 0.15 | 4.05 | 4.39 | 0.66 | -3.62 | -3.94 |
Cytoplasmic Foci | 1.94 | 6.40 | 4.35 | 1.37 | -4.37 | -1.19 | 2.97 | 2.11 | 2.30 | -0.95 | 0.38 | 8.99 | 4.65 | 2.58 | -5.32 |
Eisosomes | 5.44 | 2.31 | 1.42 | -3.15 | -0.24 | -2.70 | -7.06 | 4.71 | 4.71 | 7.91 | 0.94 | -3.40 | 3.53 | 1.54 | 5.77 |
Endoplasmic Reticulum | 0.25 | -1.92 | -4.33 | -3.83 | -3.19 | -0.06 | -7.93 | -1.26 | -1.32 | 7.43 | 0.04 | -5.80 | -4.36 | -3.83 | 1.23 |
Endosome | 0.69 | 3.21 | -1.11 | -2.12 | -6.26 | -1.80 | -1.44 | -3.56 | 0.45 | -2.00 | -1.28 | 3.76 | -3.17 | -0.58 | -7.55 |
Golgi | 1.88 | 5.49 | 2.78 | 0.42 | -3.18 | -1.76 | 4.37 | -1.27 | 1.07 | -2.57 | -1.23 | 7.31 | -0.15 | 1.05 | -3.36 |
Lipid Particles | 7.96 | 20.51 | 19.41 | 7.12 | -6.99 | -0.66 | 12.11 | 15.92 | 7.22 | 2.10 | 4.54 | 26.31 | 24.85 | 10.06 | -5.84 |
Mitochondria | 1.65 | 5.03 | 4.00 | 0.98 | -1.40 | -1.49 | 6.22 | 6.57 | 2.85 | 1.55 | -0.40 | 7.17 | 6.77 | 2.98 | 0.08 |
Mitotic Spindle | -4.52 | -19.04 | -21.55 | -13.04 | -6.13 | -1.73 | -3.98 | -25.61* | -19.40 | -14.24 | -4.09 | -17.58 | -32.84 | -23.10 | -14.40 |
None | 5.46 | 4.50 | 3.68 | -3.10 | -1.04 | 4.60 | 2.98 | 7.54 | 2.60 | 2.24 | 7.41 | 7.73 | 8.00 | 0.41 | 0.93 |
Nuclear Periphery | 0.19 | -18.04 | -10.94 | -9.00 | 6.63 | -3.25 | -4.81 | -9.39 | -3.16 | -0.11 | -2.23 | -20.17 | -14.45 | -8.43 | 7.72 |
Nuclear Periphery Foci | -5.25 | 19.75 | 12.14 | 14.89 | -6.84 | 3.41 | 10.25 | 16.96 | 6.90 | -0.48 | -0.96 | 24.89 | 20.63 | 14.89 | -5.52 |
Nucleolus | 7.05 | -11.37 | 4.48 | -3.76 | 16.94 | 0.23 | -3.09 | 10.85 | 5.78 | 8.13 | 3.96 | -10.79 | 11.10 | 3.67 | 20.28 |
Nucleus | 4.06 | -16.73 | -3.88 | -7.19 | 12.76 | -2.09 | -8.53 | 5.24 | 4.30 | 9.63 | 0.36 | -18.67 | 0.25 | -0.20 | 18.58 |
Peroxisomes | 2.97 | 4.85 | 2.00 | -0.95 | -2.46 | -2.13 | -3.07 | 0.52 | 2.22 | 2.90 | -0.63 | 4.45 | 1.82 | 1.57 | -1.52 |
Vacuole | -0.30 | -4.07 | -6.72 | -6.60 | -4.06 | -1.85 | -3.99 | -7.16 | -3.99 | 2.05 | -1.90 | -5.67 | -9.83 | -7.10 | -2.62 |
Vacuole Periphery | -1.20 | -0.09 | -3.56 | -1.98 | -3.53 | -1.77 | -0.43 | -2.83 | 0.89 | -2.79 | -2.02 | -1.10 | -4.46 | 0.22 | -4.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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