Standard name
Human Ortholog
Description DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; relocalizes to the cytosol in response to hypoxia; not essential for viability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0.06 0.12 0.16 0.11 0.1 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0 0 0 0.05 0.09 0.08 0.4 0.26 0.24 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.96 0.93 0.95 0.92 0.92 0.9 0.88 0.83 0.82 0.81 0.8 0.87 0.86 0.8 0.76 0.78 0.87 0.95 0.91 0.92 0.85 0.74 0.46
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.1 0.1 0.11 0.12 0 0 0 0.05 0 0.07 0 0 0 0 0.05 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0.06 0.14 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 3 3 2 2 5 0 0 0 0 0 0 0 2 1 0 0 2
Bud Neck 0 0 0 0 0 0 1 5 2 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 2 2 6 9 10 16 17 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 5 8 6 12 11 11 19 7 14 12 12 6 23 33 7 6 2 0 1 1 1 3 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 3
Endosome 0 1 2 0 4 0 3 1 0 0 0 0 1 0 1 2 1 0 0 2 2 1 0 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2 19 1 1 0 18 32 33 127 103 84 131 1 1 1 0 1 2 1 1 0 0 0 3
Nucleus 116 232 231 186 233 308 319 353 265 323 284 333 82 163 165 48 47 65 123 224 230 85 113 79
Nuclear Periphery 0 0 0 0 1 4 3 2 4 6 2 6 0 1 1 0 0 2 0 0 0 0 1 2
Nucleolus 4 0 3 1 0 6 11 15 31 40 39 49 1 1 2 3 0 5 0 2 2 2 7 19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 1 1 3 9 6 3 5 3 8 0 1 3 0 0 1 0 1 0 0 2 3
Vac/Vac Membrane 3 0 4 1 7 2 6 6 2 4 5 2 4 3 7 3 4 2 2 6 8 6 21 41
Unique Cell Count 123 242 248 195 254 333 355 401 321 396 349 418 94 189 206 63 60 75 130 247 250 101 154 172
Labelled Cell Count 129 258 250 196 258 355 397 449 453 508 447 563 95 193 216 64 60 81 130 247 250 101 154 172


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 6.1 8.3 7.8 7.4 5.8 5.5 5.8 4.6 4.9 5.0 4.8 7.8 7.4 7.1 8.8 10.0 10.3 9.1 9.2 8.7
Std Deviation (1e-4) 2.8 1.1 1.4 1.4 1.6 1.1 1.2 1.4 1.1 1.1 1.1 0.9 1.6 1.5 1.7 4.1 3.1 3.0 1.4 1.4 1.8
Intensity Change (Log2) – – – -0.08 -0.17 -0.52 -0.6 -0.51 -0.85 -0.74 -0.74 -0.8 -0.09 -0.17 -0.21 0.1 0.28 0.32 0.14 0.15 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 3.4 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 0.9 0.1 -0.1 0.9 -0.8 0.2 0.1 -0.3 0 3.6 4.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 4.6 4.3 0 8.6 8.2 0 0 0 0 0 0 0
Nucleus 1.0 -0.6 -0.3 -1.4 -2.1 -3.7 -4.1 -4.1 -4.7 -1.8 -2.4 -4.1 -4.0 -3.5 -1.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1.5 1.9 0 4.4 4.7 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.9 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9038 3.1198 3.2204 2.9352 3.2519 3.0399 6.4392 7.2953 7.4512 6.7496 6.5695 6.8108 4.5174 4.8003 4.4368 4.6359 3.8484 4.5037
Actin 0 0 0 0 0 0.0001 0.0012 0 0.0055 0 0.0007 0.0002 0.0105 0 0.008 0.0012 0.0025 0.0012
Bud 0 0 0 0 0 0 0.0002 0 0.0003 0.0001 0.0002 0.0001 0.0002 0 0.0006 0.0001 0.0002 0
Bud Neck 0 0 0 0 0.0002 0.0004 0.0005 0 0.001 0.0002 0.0011 0.0006 0.0008 0 0.0014 0.0001 0.0002 0.0003
Bud Periphery 0 0 0 0 0 0 0.0004 0 0.0006 0.0002 0.0006 0.0001 0.0003 0 0.0013 0.0001 0.0006 0.0001
Bud Site 0 0 0 0 0 0 0.0003 0 0.0023 0.0002 0.0003 0.0001 0.0019 0 0.0033 0.001 0.0015 0
Cell Periphery 0 0 0 0 0 0 0.0001 0 0.0003 0.0001 0.0002 0 0.0001 0 0.0004 0 0.0002 0
Cytoplasm 0 0.0001 0 0 0 0.0001 0.0175 0 0.0138 0.0001 0.0008 0.0013 0.0002 0.0001 0.0004 0.0001 0.0001 0.0002
Cytoplasmic Foci 0 0 0 0 0 0.002 0.0023 0 0.0104 0 0.0002 0.0012 0.0123 0 0.0062 0.0075 0.0011 0.0001
Eisosomes 0 0 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0002 0.0002
Endoplasmic Reticulum 0 0 0 0 0 0 0.007 0 0.0063 0 0.0015 0.0003 0.0003 0 0.0004 0 0.0002 0.0001
Endosome 0 0 0 0 0 0.001 0.0224 0 0.0218 0.0001 0.0008 0.0083 0.0081 0 0.0042 0.006 0.0022 0.0002
Golgi 0 0 0 0 0 0.0003 0.0042 0 0.0055 0 0.0001 0.0004 0.006 0 0.0038 0.0059 0.0024 0.0001
Lipid Particles 0 0 0 0 0 0.0005 0.0026 0 0.0217 0 0.0006 0.0004 0.0218 0 0.0106 0.004 0.0447 0.0002
Mitochondria 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0018 0 0.0045 0.0006 0.0025 0.0008 0.0033 0.0001 0.0124 0.0034 0.042 0.0003
None 0 0 0 0 0 0.0001 0.0065 0 0.007 0 0.0004 0.0003 0.0003 0 0.0006 0 0.0002 0.0044
Nuclear Periphery 0.0001 0.001 0.0006 0.0006 0.0004 0.0002 0.019 0.0007 0.0132 0.0007 0.0286 0.0038 0.0013 0.0026 0.0015 0.0002 0.0006 0.0005
Nucleolus 0.0105 0.0099 0.0071 0.0057 0.0484 0.0261 0.0099 0.0057 0.0052 0.0352 0.0329 0.0225 0.0098 0.0109 0.0057 0.007 0.0256 0.0217
Nucleus 0.9891 0.9868 0.992 0.9933 0.9501 0.9678 0.8952 0.9934 0.8662 0.9614 0.9214 0.9555 0.9043 0.9858 0.9274 0.9569 0.8686 0.9696
Peroxisomes 0 0 0 0 0 0.0006 0.0002 0 0.0028 0 0.0001 0.0005 0.0132 0 0.0063 0.0059 0.0047 0.0001
Punctate Nuclear 0.0001 0.002 0.0002 0.0001 0.0007 0.0007 0.0043 0.0001 0.007 0.0001 0.0041 0.0028 0.0043 0.0001 0.0022 0.0002 0.0003 0.0003
Vacuole 0 0 0 0 0 0 0.0034 0 0.0034 0.0008 0.0014 0.0004 0.0005 0.0001 0.0018 0.0002 0.0009 0.0001
Vacuole Periphery 0 0 0 0 0 0 0.0011 0 0.0013 0.0003 0.0018 0.0004 0.0004 0.0001 0.0015 0.0001 0.0009 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.2808 34.3317 27.4353 28.3327 37.3453 22.1444 25.0332 24.3299 21.4018 26.3078
Translational Efficiency 1.4074 1.1583 1.2191 1.025 1.0794 1.6409 0.9461 1.242 1.037 1.2199

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1317 1066 2231 1741 1802 2233 246 1778 3119 3299 2477 3519

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 708.54 914.99 1007.68 1018.04 818.20 1009.10 1131.50 1101.60 771.90 978.69 1019.98 1060.26
Standard Deviation 156.23 155.27 176.54 160.59 114.08 137.05 154.56 183.28 144.08 149.80 178.37 177.42
Intensity Change Log 2 — 0.368907 0.508116 0.522873 — 0.302544 0.467711 0.429075 — 0.333722 0.486603 0.473365

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000366 0.000602 0.000437 0.000993 0.000200 0.000273 0.000960 0.000577 0.000270 0.000379 0.000489 0.000783
Bud Neck 0.002942 0.007393 0.016339 0.022602 0.004098 0.007127 0.008570 0.016888 0.003610 0.007213 0.015567 0.019715
Bud Site 0.001879 0.000954 0.000888 0.005251 0.000415 0.001508 0.000756 0.001263 0.001033 0.001329 0.000874 0.003236
Cell Periphery 0.000495 0.000191 0.000090 0.000226 0.000098 0.000238 0.000036 0.000086 0.000266 0.000223 0.000084 0.000156
Cytoplasm 0.016537 0.002152 0.001254 0.017124 0.001550 0.001811 0.000266 0.002678 0.007878 0.001921 0.001156 0.009825
Cytoplasmic Foci 0.002555 0.000143 0.000011 0.002227 0.000307 0.000258 0.000016 0.000023 0.001256 0.000220 0.000011 0.001113
Eisosomes 0.000034 0.000032 0.000029 0.000034 0.000017 0.000039 0.000044 0.000028 0.000024 0.000037 0.000031 0.000031
Endoplasmic Reticulum 0.005321 0.006225 0.002806 0.004685 0.003517 0.006276 0.004927 0.003338 0.004279 0.006260 0.003017 0.004005
Endosome 0.000563 0.000313 0.000088 0.003335 0.000213 0.001248 0.000069 0.000116 0.000361 0.000946 0.000086 0.001708
Golgi 0.000387 0.000072 0.000035 0.001493 0.000063 0.000213 0.000048 0.000042 0.000200 0.000168 0.000036 0.000760
Lipid Particles 0.000343 0.000077 0.000013 0.000918 0.000082 0.000240 0.000050 0.000028 0.000192 0.000187 0.000017 0.000469
Mitochondria 0.001001 0.000755 0.000854 0.004242 0.000368 0.001888 0.001092 0.001333 0.000636 0.001522 0.000877 0.002772
Mitotic Spindle 0.000413 0.000088 0.000213 0.030525 0.000540 0.001508 0.000039 0.000742 0.000486 0.001050 0.000196 0.015477
None 0.051679 0.004020 0.001446 0.011178 0.003906 0.002806 0.000810 0.001974 0.024078 0.003198 0.001382 0.006528
Nuclear Periphery 0.000409 0.000322 0.000238 0.002568 0.000237 0.000922 0.000169 0.000397 0.000310 0.000728 0.000231 0.001471
Nuclear Periphery Foci 0.001869 0.000545 0.000034 0.001454 0.000174 0.000450 0.000027 0.000153 0.000890 0.000481 0.000034 0.000797
Nucleolus 0.019847 0.016707 0.005125 0.008665 0.017067 0.014193 0.009989 0.005104 0.018241 0.015005 0.005608 0.006866
Nucleus 0.891098* 0.958194* 0.969524* 0.873652* 0.966364* 0.954948* 0.970291* 0.963977* 0.934583* 0.955997* 0.969600* 0.919289*
Peroxisomes 0.000661 0.000369 0.000072 0.000598 0.000118 0.000263 0.000439 0.000092 0.000347 0.000297 0.000108 0.000342
Vacuole 0.001220 0.000670 0.000419 0.006401 0.000512 0.002654 0.001305 0.001030 0.000811 0.002013 0.000507 0.003687
Vacuole Periphery 0.000378 0.000177 0.000085 0.001828 0.000154 0.001136 0.000097 0.000132 0.000249 0.000826 0.000086 0.000971

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.87 -2.65 -11.81 -5.86 -11.26 -3.14 -13.45 -13.22 -10.96 4.84 -3.42 -10.90 -17.16 -12.36 -10.67
Bud Neck -6.65 -15.86 -15.36 -11.57 -4.39 -4.16 -4.70 -15.99 -11.75 -7.33 -6.81 -15.86 -21.68 -16.12 -5.01
Bud Site 1.77 1.74 -4.19 -6.84 -6.98 -3.69 -3.58 -6.55 -0.73 -4.29 -0.94 0.21 -6.34 -5.75 -7.68
Cell Periphery 4.26 6.57 5.17 0.45 -4.86 -2.52 3.30 0.58 2.83 -5.24 0.97 6.26 4.74 2.41 -3.89
Cytoplasm 6.74 7.43 0.56 -7.91 -8.97 -0.26 3.31 -2.16 -1.55 -5.24 5.91 7.39 -0.89 -7.63 -9.55
Cytoplasmic Foci 4.31 4.63 2.37 -3.44 -4.16 0.54 1.64 1.57 3.34 -2.04 4.05 4.87 2.45 -2.56 -4.21
Eisosomes 0.73 1.85 -0.15 -1.28 -3.59 -8.56 -8.55 -8.45 3.72 4.69 -5.50 -3.47 -4.67 2.30 -1.27
Endoplasmic Reticulum -1.79 7.81 0.72 2.43 -7.53 -7.92 -1.80 0.32 8.35 1.90 -6.74 6.19 0.21 6.96 -6.09
Endosome 2.02 5.11 -3.16 -3.81 -4.45 -3.16 2.78 1.52 3.44 -3.39 -2.51 5.65 -3.09 -0.33 -4.55
Golgi 1.68 1.88 -1.22 -2.49 -2.62 -1.81 0.38 0.31 2.02 -0.31 0.42 2.00 -1.38 -1.75 -2.66
Lipid Particles 3.48 4.51 -1.26 -3.00 -3.43 -1.25 0.98 1.07 1.62 0.35 0.16 4.82 -1.27 -1.13 -3.57
Mitochondria 0.78 0.30 -5.26 -7.36 -6.96 -3.13 -7.86 -8.22 0.55 -2.62 -2.50 -2.08 -8.00 -2.83 -7.96
Mitotic Spindle 1.33 0.45 -8.47 -8.61 -8.53 -1.40 1.08 -1.00 0.65 -3.11 -1.25 0.74 -8.40 -7.91 -8.68
None 10.75 11.54 9.56 -3.75 -7.35 1.37 4.15 2.45 1.68 -4.25 10.62 11.75 9.31 -3.54 -7.76
Nuclear Periphery 0.51 1.74 -5.04 -5.08 -5.58 -2.44 0.81 -3.37 1.51 -4.72 -2.15 1.13 -5.71 -2.38 -6.17
Nuclear Periphery Foci 2.25 3.61 1.91 -0.92 -4.63 -1.64 2.49 0.15 1.63 -1.65 1.83 3.89 1.65 -0.49 -4.86
Nucleolus 1.80 10.03 5.95 3.87 -4.14 1.90 3.38 7.31 6.70 2.09 2.88 11.85 9.07 6.88 -2.88
Nucleus -10.53 -12.61 0.99 13.25 15.93 3.36 -0.76 3.69 -0.19 3.93 -6.39 -10.77 3.65 10.71 15.64
Peroxisomes 0.93 1.98 0.91 -0.01 -3.48 -2.62 -6.73 0.05 2.68 6.93 0.40 1.87 0.75 0.80 -3.06
Vacuole 2.48 3.99 -7.41 -8.10 -8.41 -3.59 -1.73 -2.70 1.14 -0.66 -2.86 1.91 -7.89 -4.63 -8.52
Vacuole Periphery 1.87 2.78 -2.53 -3.51 -3.88 -3.51 1.23 0.36 3.59 -1.19 -2.97 3.18 -2.59 0.99 -4.04
Standard name
Human Ortholog
Description DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; relocalizes to the cytosol in response to hypoxia; not essential for viability
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Dat1

Dat1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dat1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available