Standard name | |
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Human Ortholog | |
Description | NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in respiratory chain but does not pump protons, in contrast to higher eukaryotic multisubunit respiratory complex I; upon apoptotic stress, is activated in mitochondria by N-terminal cleavage, then translocates to cytoplasm to induce apoptosis; homolog of human AIFM2; yeast NDI1 complements several phenotypes of human cell line with mutated MT-ND4, implicated in Leber hereditary optic neuropathy |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 5 | 3 | 0 | 6 | 4 | 2 | 6 | 8 | 6 | 3 | 4 | 18 |
Bud | 0 | 0 | 4 | 1 | 0 | 0 | 3 | 5 | 4 | 5 | 2 | 4 | 3 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 8 | 5 | 0 | 1 | 0 | 3 | 0 | 1 | 0 | 0 | 2 | 4 | 3 | 1 | 19 | 14 | 8 | 0 | 2 | 1 | 0 | 0 | 1 |
Cytoplasm | 2 | 1 | 2 | 2 | 0 | 2 | 2 | 6 | 2 | 3 | 2 | 0 | 1 | 4 | 1 | 18 | 4 | 6 | 0 | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 2 | 3 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 3 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 10 | 2 | 1 | 1 | 1 | 2 | 0 | 1 | 1 |
Golgi | 0 | 19 | 4 | 1 | 11 | 0 | 2 | 2 | 0 | 1 | 0 | 2 | 19 | 16 | 24 | 84 | 114 | 64 | 14 | 22 | 16 | 10 | 9 | 14 |
Mitochondria | 220 | 218 | 175 | 113 | 118 | 192 | 233 | 236 | 197 | 256 | 183 | 236 | 201 | 229 | 214 | 27 | 29 | 10 | 186 | 213 | 149 | 74 | 110 | 111 |
Nucleus | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 21 | 4 | 10 | 1 | 11 | 8 | 21 | 13 | 19 | 22 | 13 | 10 | 3 | 4 | 3 | 9 | 11 | 2 | 2 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 24 | 18 | 9 | 8 | 11 | 19 | 48 | 47 | 33 | 43 | 29 | 31 | 5 | 4 | 3 | 22 | 9 | 6 | 1 | 2 | 0 | 0 | 1 | 4 |
Peroxisomes | 1 | 3 | 5 | 3 | 0 | 2 | 3 | 2 | 4 | 1 | 3 | 0 | 3 | 4 | 4 | 18 | 15 | 5 | 6 | 6 | 13 | 4 | 5 | 11 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 9 | 15 | 6 | 3 | 5 | 3 | 15 | 14 | 5 | 6 | 4 | 10 | 23 | 20 | 17 | 51 | 41 | 26 | 2 | 11 | 2 | 3 | 3 | 1 |
Unique Cell Count | 229 | 246 | 187 | 120 | 132 | 206 | 263 | 267 | 214 | 274 | 192 | 243 | 218 | 250 | 232 | 229 | 209 | 116 | 223 | 270 | 200 | 99 | 142 | 174 |
Labelled Cell Count | 281 | 293 | 226 | 132 | 161 | 228 | 332 | 328 | 268 | 339 | 236 | 300 | 269 | 289 | 269 | 271 | 252 | 134 | 223 | 270 | 200 | 99 | 142 | 174 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.5 | 21.2 | 18.1 | 16.7 | 20.6 | 18.9 | 19.3 | 19.0 | 18.6 | 17.9 | 18.8 | 18.9 | 19.3 | 18.5 | 19.6 | 17.5 | 22.9 | 23.5 | 17.0 | 17.7 | 18.1 |
Std Deviation (1e-4) | 4.1 | 5.9 | 5.0 | 4.6 | 4.3 | 5.1 | 6.1 | 5.7 | 6.4 | 6.6 | 7.2 | 7.7 | 3.8 | 4.3 | 4.3 | 5.3 | 6.5 | 7.8 | 4.4 | 4.3 | 4.5 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.18 | 0.06 | 0.09 | 0.07 | 0.03 | -0.02 | 0.05 | 0.06 | 0.09 | 0.02 | 0.11 | -0.06 | 0.33 | 0.37 | -0.09 | -0.04 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 1.9 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 2.1 | 3.3 | 8.6 | 11.4 | 10.8 |
Mitochondria | 0.2 | -1.3 | -0.2 | -1.8 | -1.9 | -0.6 | -0.1 | 0.7 | 1.8 | -0.5 | -0.8 | -0.5 | -16.6 | -15.8 | -14.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1.1 | -0.7 | 1.1 | -0.2 | 1.4 | 1.1 | 0.6 | -0.6 | -2.3 | -2.2 | -2.4 | -0.7 | 0 | 0 |
Nucleolus | 0.7 | 1.3 | 1.7 | 4.2 | 4.1 | 3.5 | 3.6 | 3.3 | 2.8 | -1.4 | -2.0 | -2.1 | 1.9 | -0.2 | 0.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 1.2 | 1.0 | -0.5 | 0 | 0 | 0.5 | 2.9 | 2.1 | 1.8 | 5.6 | 5.0 | 5.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0141 | 0.0012 | 0.0011 | 0.0031 | 0.0002 | 0.001 | 0.0333 | 0.0095 | 0.0127 | 0.0068 | 0.0013 | 0.0198 | 0.1971 | 0.0405 | 0.0398 | 0.0055 | 0.0002 | 0.0115 |
Bud | 0.0014 | 0.0001 | 0 | 0.0016 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0009 | 0.0009 | 0.0003 | 0.0001 | 0 | 0 |
Bud Neck | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 |
Bud Periphery | 0.009 | 0.0002 | 0 | 0.0028 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.002 | 0.0013 | 0.0004 | 0.0002 | 0 | 0.0001 |
Bud Site | 0.0004 | 0.0003 | 0 | 0.0008 | 0 | 0 | 0.0005 | 0.0011 | 0.0002 | 0.0003 | 0 | 0 | 0.0025 | 0.0014 | 0.0008 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0009 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Cytoplasmic Foci | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0008 | 0.0036 | 0.0037 | 0.0008 | 0.0035 | 0.0006 | 0.0003 | 0.0086 | 0.0024 | 0.0003 | 0.002 | 0 | 0.0004 |
Eisosomes | 0.0022 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.001 | 0.0011 | 0.0005 | 0.0002 | 0.0003 | 0.001 | 0.0013 | 0.0009 | 0.0013 | 0.0004 | 0.0001 | 0.0003 |
Endoplasmic Reticulum | 0.0035 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 |
Endosome | 0.0019 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0004 | 0.0014 | 0.0009 | 0.0013 | 0.0037 | 0.0003 | 0.0004 | 0.0053 | 0.0064 | 0.0002 | 0.0264 | 0 | 0.0011 |
Golgi | 0.0023 | 0.0007 | 0.0006 | 0.0003 | 0.0001 | 0.0003 | 0.0025 | 0.0086 | 0.0059 | 0.0122 | 0.0012 | 0.0027 | 0.0113 | 0.0518 | 0.0016 | 0.105 | 0.0002 | 0.0097 |
Lipid Particles | 0.0068 | 0.0029 | 0.0031 | 0.0036 | 0.0015 | 0.002 | 0.0085 | 0.0347 | 0.0095 | 0.0188 | 0.02 | 0.0062 | 0.0487 | 0.0516 | 0.0017 | 0.0086 | 0.0014 | 0.0099 |
Mitochondria | 0.3613 | 0.9783 | 0.9894 | 0.853 | 0.9959 | 0.9884 | 0.9281 | 0.9195 | 0.9584 | 0.9397 | 0.9648 | 0.9615 | 0.6067 | 0.7706 | 0.9494 | 0.8409 | 0.996 | 0.9521 |
None | 0.0032 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.4093 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0007 | 0.0002 | 0 | 0.0001 | 0 | 0.0011 |
Nucleolus | 0.0016 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0008 | 0.0004 | 0.0052 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0007 | 0.0036 | 0.0001 | 0.0002 | 0 | 0.0002 |
Nucleus | 0.0044 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 |
Peroxisomes | 0.001 | 0.0007 | 0.002 | 0.0049 | 0.0011 | 0.0013 | 0.0114 | 0.0091 | 0.0041 | 0.0111 | 0.0105 | 0.0053 | 0.0601 | 0.0057 | 0.0033 | 0.0042 | 0.0013 | 0.0025 |
Punctate Nuclear | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0022 | 0 | 0 | 0.0005 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.006 | 0.0026 | 0.0004 | 0.0088 | 0.0001 | 0.0009 | 0.0007 | 0.0008 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0144 | 0.0219 | 0.0002 | 0.001 | 0.0001 | 0.0009 |
Vacuole Periphery | 0.1703 | 0.0111 | 0.0026 | 0.1195 | 0.0009 | 0.0026 | 0.005 | 0.0041 | 0.0058 | 0.0018 | 0.0005 | 0.0021 | 0.0374 | 0.0386 | 0.0005 | 0.0045 | 0.0007 | 0.0099 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0179 | 3.838 | 9.4933 | 22.597 | 12.5248 | 22.1703 | 20.535 | 25.7552 | 31.5592 | 19.5542 |
Translational Efficiency | 1.068 | 3.0132 | 1.8116 | 1.2948 | 1.3968 | 0.8922 | 1.06 | 1.2868 | 1.2621 | 1.2453 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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