Standard name | |
---|---|
Human Ortholog | |
Description | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 47 | 15 | 13 | 5 | 4 | 6 | 0 | 2 | 0 | 0 | 2 | 4 | 2 | 1 | 22 | 7 | 10 | 7 | 14 | 19 | 16 | 13 | 25 |
Bud | 1 | 2 | 5 | 3 | 8 | 8 | 6 | 4 | 15 | 8 | 4 | 5 | 1 | 0 | 1 | 2 | 0 | 3 | 8 | 20 | 14 | 16 | 22 | 23 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 5 | 2 | 4 | 5 | 7 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 4 | 3 | 1 | 5 | 13 |
Bud Site | 1 | 0 | 0 | 1 | 0 | 7 | 4 | 4 | 18 | 24 | 6 | 11 | 0 | 0 | 0 | 1 | 0 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 33 | 74 | 40 | 23 | 20 | 9 | 10 | 17 | 9 | 6 | 3 | 8 | 57 | 86 | 76 | 70 | 39 | 43 | 27 | 60 | 24 | 21 | 31 | 24 |
Endoplasmic Reticulum | 8 | 16 | 8 | 2 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 5 | 4 | 12 | 4 | 3 | 5 | 4 | 3 | 4 |
Endosome | 185 | 156 | 166 | 120 | 123 | 149 | 142 | 104 | 56 | 49 | 27 | 22 | 146 | 254 | 208 | 133 | 50 | 60 | 65 | 104 | 70 | 43 | 72 | 80 |
Golgi | 4 | 29 | 7 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 8 | 2 | 0 | 36 | 32 | 24 | 7 | 5 | 3 | 4 | 44 | 25 |
Mitochondria | 14 | 0 | 8 | 8 | 13 | 39 | 51 | 71 | 126 | 98 | 49 | 55 | 1 | 0 | 0 | 49 | 12 | 25 | 2 | 5 | 11 | 1 | 6 | 11 |
Nucleus | 0 | 0 | 2 | 1 | 0 | 1 | 2 | 7 | 3 | 7 | 1 | 2 | 0 | 0 | 0 | 6 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 3 | 2 | 2 |
Nucleolus | 63 | 59 | 48 | 49 | 89 | 146 | 195 | 198 | 202 | 198 | 97 | 133 | 26 | 69 | 116 | 321 | 141 | 171 | 2 | 7 | 2 | 1 | 1 | 1 |
Peroxisomes | 2 | 1 | 6 | 7 | 14 | 20 | 45 | 33 | 34 | 37 | 9 | 16 | 3 | 11 | 37 | 18 | 5 | 11 | 1 | 3 | 3 | 3 | 12 | 14 |
SpindlePole | 34 | 60 | 68 | 63 | 103 | 104 | 163 | 121 | 106 | 145 | 57 | 68 | 36 | 91 | 108 | 332 | 138 | 126 | 168 | 203 | 160 | 77 | 119 | 106 |
Vac/Vac Membrane | 31 | 64 | 31 | 11 | 23 | 5 | 13 | 22 | 7 | 3 | 5 | 4 | 10 | 22 | 29 | 52 | 36 | 28 | 3 | 8 | 4 | 1 | 3 | 2 |
Unique Cell Count | 285 | 389 | 306 | 216 | 270 | 331 | 407 | 415 | 395 | 380 | 170 | 229 | 229 | 391 | 369 | 662 | 301 | 347 | 338 | 473 | 359 | 204 | 358 | 352 |
Labelled Cell Count | 384 | 508 | 406 | 308 | 400 | 498 | 642 | 588 | 585 | 583 | 262 | 330 | 292 | 539 | 576 | 1048 | 466 | 521 | 338 | 473 | 359 | 204 | 358 | 352 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.2 | 14.2 | 11.2 | 10.9 | 10.5 | 9.9 | 9.5 | 9.2 | 9.2 | 9.1 | 9.1 | 8.9 | 12.2 | 11.7 | 11.1 | 16.1 | 17.5 | 18.9 | 10.9 | 10.6 | 11.4 |
Std Deviation (1e-4) | 2.1 | 2.3 | 2.5 | 2.1 | 2.2 | 1.9 | 2.1 | 2.0 | 2.2 | 2.3 | 2.3 | 2.5 | 2.2 | 2.0 | 2.2 | 4.1 | 4.9 | 6.0 | 2.0 | 2.1 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.1 | -0.18 | -0.24 | -0.29 | -0.28 | -0.31 | -0.3 | -0.33 | 0.12 | 0.06 | -0.01 | 0.52 | 0.64 | 0.75 | -0.04 | -0.08 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.6 | -2.0 | -2.7 | -2.7 | -4.6 | -3.8 | -4.4 | -2.9 | -2.6 | -2.0 | -3.7 | -3.9 | -1.2 | -1.7 | -1.3 |
Bud | 0 | 1.1 | 0.7 | 0 | 0 | 1.7 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3.8 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.8 | -2.2 | -4.9 | -5.5 | -4.4 | -5.6 | -6.0 | -4.1 | -3.8 | 3.5 | 3.0 | 2.6 | -1.1 | 0 | -0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | 0.6 |
Endosome | 0.3 | -2.1 | -2.3 | -5.2 | -8.0 | -11.3 | -11.6 | -8.2 | -10.7 | 2.2 | 2.9 | 0.6 | -10.7 | -9.7 | -9.9 |
Golgi | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 2.2 | 4.2 | 2.8 |
Mitochondria | 0.7 | 1.4 | 4.4 | 4.8 | 6.2 | 9.8 | 8.3 | 8.4 | 7.6 | 0 | 0 | 0 | 2.9 | 0.9 | 2.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.0 | 4.9 | 7.8 | 9.0 | 9.0 | 9.7 | 9.9 | 9.4 | 10.3 | -1.4 | 0.7 | 4.7 | 9.8 | 8.3 | 9.1 |
Peroxisomes | 0.9 | 2.1 | 2.6 | 4.7 | 3.5 | 3.8 | 4.2 | 2.0 | 2.9 | 0 | 0.7 | 4.3 | 0.7 | -0.3 | 1.0 |
SpindlePole | 1.8 | 4.2 | 2.6 | 5.0 | 2.1 | 1.4 | 4.5 | 2.7 | 2.0 | -1.9 | 0.3 | 2.1 | 8.2 | 6.1 | 3.9 |
Vacuole | -2.1 | -0.7 | -4.7 | -3.8 | -2.5 | -4.8 | -5.6 | -2.8 | -3.9 | -2.5 | -2.2 | -1.0 | -1.2 | 0.7 | -0.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.1533 | 8.4021 | 7.0524 | 6.9938 | 6.8383 | 7.2086 | 7.5777 | 10.3956 | 9.7306 | 8.8256 | 8.7324 | 10.2477 | 10.04 | 12.922 | 11.9124 | 11.2881 | 10.9178 | 12.203 |
Actin | 0.0069 | 0.0053 | 0.003 | 0.005 | 0.0252 | 0.0042 | 0.0488 | 0.0113 | 0.0316 | 0.0038 | 0.0631 | 0.0137 | 0.0186 | 0.002 | 0.0081 | 0.0121 | 0.0103 | 0.0065 |
Bud | 0.001 | 0.0017 | 0.0005 | 0.0004 | 0.001 | 0.0005 | 0.0007 | 0.0009 | 0.0023 | 0.0009 | 0.0016 | 0.0045 | 0.0004 | 0.0015 | 0.0008 | 0.0011 | 0.0006 | 0.0006 |
Bud Neck | 0.0043 | 0.002 | 0.0108 | 0.0025 | 0.0439 | 0.0118 | 0.0052 | 0.002 | 0.0032 | 0.0019 | 0.0059 | 0.0027 | 0.0022 | 0.0028 | 0.0055 | 0.0258 | 0.0058 | 0.0089 |
Bud Periphery | 0.0009 | 0.0006 | 0.0006 | 0.0003 | 0.0008 | 0.0002 | 0.0009 | 0.0003 | 0.0008 | 0.0022 | 0.0011 | 0.0012 | 0.0003 | 0.0005 | 0.0007 | 0.0007 | 0.0004 | 0.0003 |
Bud Site | 0.0082 | 0.0369 | 0.0102 | 0.0013 | 0.0024 | 0.0007 | 0.0087 | 0.012 | 0.0026 | 0.002 | 0.0039 | 0.0013 | 0.0052 | 0.0155 | 0.0091 | 0.0024 | 0.0012 | 0.001 |
Cell Periphery | 0.0006 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0741 | 0.2201 | 0.0456 | 0.1117 | 0.3783 | 0.2772 | 0.0586 | 0.1393 | 0.0669 | 0.0302 | 0.22 | 0.1668 | 0.1202 | 0.3164 | 0.1024 | 0.1361 | 0.2499 | 0.214 |
Cytoplasmic Foci | 0.383 | 0.2618 | 0.3696 | 0.3451 | 0.163 | 0.294 | 0.3507 | 0.3939 | 0.3533 | 0.3864 | 0.2164 | 0.3877 | 0.2768 | 0.1888 | 0.2598 | 0.1917 | 0.1835 | 0.2722 |
Eisosomes | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0005 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.016 | 0.0042 | 0.002 | 0.0052 | 0.031 | 0.0064 | 0.0033 | 0.0023 | 0.0014 | 0.003 | 0.0156 | 0.0037 | 0.0099 | 0.0023 | 0.0016 | 0.0049 | 0.0121 | 0.0049 |
Endosome | 0.0717 | 0.0145 | 0.0099 | 0.0687 | 0.1905 | 0.0665 | 0.084 | 0.0424 | 0.0276 | 0.026 | 0.1417 | 0.0768 | 0.0833 | 0.0268 | 0.0259 | 0.0376 | 0.1557 | 0.0631 |
Golgi | 0.023 | 0.0047 | 0.0034 | 0.0171 | 0.0202 | 0.0129 | 0.0181 | 0.0061 | 0.0068 | 0.007 | 0.0288 | 0.0142 | 0.0095 | 0.0048 | 0.0061 | 0.01 | 0.0239 | 0.0095 |
Lipid Particles | 0.0173 | 0.0166 | 0.004 | 0.0118 | 0.0082 | 0.0087 | 0.0435 | 0.0019 | 0.0058 | 0.0169 | 0.0232 | 0.0084 | 0.0132 | 0.0032 | 0.0067 | 0.0132 | 0.041 | 0.0055 |
Mitochondria | 0.0042 | 0.0008 | 0.0016 | 0.0028 | 0.0095 | 0.0026 | 0.0062 | 0.0006 | 0.0018 | 0.0489 | 0.016 | 0.0043 | 0.0016 | 0.0009 | 0.0043 | 0.0083 | 0.0049 | 0.0041 |
None | 0.0018 | 0.002 | 0.0086 | 0.0012 | 0.0021 | 0.0014 | 0.0102 | 0.002 | 0.002 | 0.0347 | 0.0046 | 0.0017 | 0.0116 | 0.0018 | 0.0032 | 0.0026 | 0.0043 | 0.0021 |
Nuclear Periphery | 0.0044 | 0.0039 | 0.0043 | 0.0055 | 0.0255 | 0.0126 | 0.0138 | 0.0039 | 0.0025 | 0.0031 | 0.0318 | 0.008 | 0.0184 | 0.0096 | 0.0038 | 0.009 | 0.0383 | 0.0129 |
Nucleolus | 0.0017 | 0.012 | 0.0063 | 0.0049 | 0.0017 | 0.001 | 0.006 | 0.0025 | 0.0016 | 0.0024 | 0.0027 | 0.0007 | 0.0048 | 0.0046 | 0.0036 | 0.0024 | 0.0048 | 0.0027 |
Nucleus | 0.0096 | 0.0245 | 0.0269 | 0.0054 | 0.0091 | 0.0266 | 0.0075 | 0.0155 | 0.0086 | 0.0035 | 0.0114 | 0.016 | 0.024 | 0.0704 | 0.031 | 0.0421 | 0.0281 | 0.0427 |
Peroxisomes | 0.0205 | 0.0061 | 0.0066 | 0.0056 | 0.0232 | 0.0056 | 0.0519 | 0.02 | 0.0252 | 0.0207 | 0.0432 | 0.0233 | 0.0109 | 0.0096 | 0.01 | 0.0134 | 0.0442 | 0.015 |
Punctate Nuclear | 0.3455 | 0.38 | 0.4839 | 0.4024 | 0.0466 | 0.2623 | 0.2753 | 0.3419 | 0.4546 | 0.4039 | 0.157 | 0.2628 | 0.3838 | 0.3348 | 0.5138 | 0.4833 | 0.1808 | 0.331 |
Vacuole | 0.0043 | 0.0017 | 0.0015 | 0.0022 | 0.0116 | 0.0035 | 0.0037 | 0.0009 | 0.0007 | 0.0015 | 0.0069 | 0.0015 | 0.0042 | 0.003 | 0.0028 | 0.0022 | 0.0068 | 0.0022 |
Vacuole Periphery | 0.0012 | 0.0003 | 0.0003 | 0.0009 | 0.0059 | 0.0012 | 0.0015 | 0.0003 | 0.0003 | 0.0006 | 0.0043 | 0.0006 | 0.0009 | 0.0005 | 0.0005 | 0.0008 | 0.0033 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 148.4401 | 162.1844 | 137.853 | 99.3164 | 97.2987 | 149.1821 | 182.6191 | 157.4653 | 135.8985 | 95.4909 |
Translational Efficiency | 0.9651 | 1.0508 | 0.9046 | 1.09 | 0.8694 | 0.7982 | 0.9211 | 0.8989 | 0.7754 | 0.8014 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
856 | 1605 | 416 | 1037 | 1938 | 2120 | 2348 | 1444 | 2794 | 3725 | 2764 | 2481 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 960.41 | 1156.18 | 1473.33 | 1552.95 | 1102.77 | 1149.13 | 1481.02 | 1651.31 | 1059.16 | 1152.17 | 1479.86 | 1610.20 |
Standard Deviation | 131.01 | 204.72 | 217.69 | 299.40 | 162.37 | 335.92 | 206.40 | 327.22 | 166.89 | 286.87 | 208.16 | 319.60 |
Intensity Change Log 2 | — | 0.267644 | 0.617358 | 0.693289 | — | 0.059410 | 0.425459 | 0.582479 | — | 0.160091 | 0.517971 | 0.635122 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000770 | 0.000425 | 0.000161 | 0.000477 | 0.000271 | 0.000267 | 0.000015 | 0.000552 | 0.000424 | 0.000335 | 0.000037 | 0.000521 |
Bud Neck | 0.003379 | 0.002791 | 0.003933 | 0.002421 | 0.003381 | 0.002826 | 0.000728 | 0.002288 | 0.003381 | 0.002811 | 0.001211 | 0.002344 |
Bud Site | 0.017536 | 0.011998 | 0.005154 | 0.008052 | 0.009386 | 0.013243 | 0.000903 | 0.010055 | 0.011883 | 0.012707 | 0.001543 | 0.009218 |
Cell Periphery | 0.000105 | 0.000078 | 0.000044 | 0.000049 | 0.000062 | 0.000136 | 0.000005 | 0.000042 | 0.000075 | 0.000111 | 0.000011 | 0.000045 |
Cytoplasm | 0.054891 | 0.052746 | 0.009473 | 0.011665 | 0.021840 | 0.076729 | 0.017677 | 0.032529 | 0.031966 | 0.066395 | 0.016442 | 0.023809 |
Cytoplasmic Foci | 0.212457* | 0.109709 | 0.014131 | 0.018859 | 0.077342 | 0.089664 | 0.002168 | 0.015230 | 0.118737 | 0.098301 | 0.003969 | 0.016747 |
Eisosomes | 0.000021 | 0.000007 | 0.000011 | 0.000003 | 0.000010 | 0.000006 | 0.000000 | 0.000003 | 0.000013 | 0.000007 | 0.000002 | 0.000003 |
Endoplasmic Reticulum | 0.000961 | 0.000362 | 0.000155 | 0.000091 | 0.000493 | 0.000710 | 0.000046 | 0.000129 | 0.000637 | 0.000560 | 0.000062 | 0.000113 |
Endosome | 0.035301 | 0.012390 | 0.001408 | 0.001895 | 0.013948 | 0.012125 | 0.000444 | 0.004324 | 0.020490 | 0.012239 | 0.000589 | 0.003309 |
Golgi | 0.012212 | 0.006685 | 0.000636 | 0.001393 | 0.003752 | 0.006308 | 0.000083 | 0.005712 | 0.006344 | 0.006470 | 0.000167 | 0.003907 |
Lipid Particles | 0.016272 | 0.005364 | 0.000775 | 0.002411 | 0.005801 | 0.003334 | 0.000145 | 0.001135 | 0.009009 | 0.004209 | 0.000240 | 0.001668 |
Mitochondria | 0.001074 | 0.001114 | 0.000243 | 0.000366 | 0.001331 | 0.001926 | 0.000054 | 0.000737 | 0.001252 | 0.001576 | 0.000082 | 0.000582 |
Mitotic Spindle | 0.555174* | 0.742612* | 0.944663* | 0.940869* | 0.802562* | 0.759007* | 0.970516* | 0.910675* | 0.726770* | 0.751943* | 0.966625* | 0.923295* |
None | 0.001400 | 0.000972 | 0.000415 | 0.000404 | 0.000408 | 0.002593 | 0.000262 | 0.000898 | 0.000712 | 0.001894 | 0.000285 | 0.000692 |
Nuclear Periphery | 0.000330 | 0.000258 | 0.000644 | 0.000358 | 0.000493 | 0.000449 | 0.000285 | 0.000599 | 0.000443 | 0.000366 | 0.000339 | 0.000498 |
Nuclear Periphery Foci | 0.065599 | 0.039332 | 0.003078 | 0.004103 | 0.046880 | 0.017482 | 0.001281 | 0.002853 | 0.052615 | 0.026897 | 0.001551 | 0.003375 |
Nucleolus | 0.003292 | 0.001612 | 0.004517 | 0.000762 | 0.001959 | 0.001502 | 0.000404 | 0.000386 | 0.002367 | 0.001550 | 0.001023 | 0.000543 |
Nucleus | 0.003804 | 0.002839 | 0.004125 | 0.001408 | 0.001422 | 0.003283 | 0.002595 | 0.002094 | 0.002152 | 0.003092 | 0.002825 | 0.001807 |
Peroxisomes | 0.009979 | 0.003670 | 0.003206 | 0.001026 | 0.005511 | 0.003101 | 0.000092 | 0.001890 | 0.006880 | 0.003346 | 0.000561 | 0.001529 |
Vacuole | 0.004309 | 0.004319 | 0.003039 | 0.003121 | 0.002745 | 0.004241 | 0.002245 | 0.006483 | 0.003224 | 0.004275 | 0.002365 | 0.005078 |
Vacuole Periphery | 0.001134 | 0.000717 | 0.000190 | 0.000266 | 0.000402 | 0.001068 | 0.000051 | 0.001386 | 0.000626 | 0.000917 | 0.000072 | 0.000918 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.14 | 2.29 | 1.26 | -0.32 | -0.39 | -0.04 | 8.07 | -1.42 | -1.40 | -2.72 | 1.30 | 7.16 | -0.73 | -1.32 | -3.34 |
Bud Neck | 0.33 | -5.92 | -1.72 | -1.96 | 4.83 | 0.99 | 3.85 | 0.04 | -0.74 | -2.91 | 1.10 | 0.76 | -0.82 | -1.74 | -1.42 |
Bud Site | 1.85 | 0.36 | 1.55 | -0.08 | 1.06 | -3.06 | 7.55 | -1.87 | 0.45 | -5.90 | -1.48 | 7.53 | -0.86 | 0.32 | -6.43 |
Cell Periphery | 2.02 | -0.50 | 0.99 | -0.44 | 1.22 | -2.43 | 8.13 | 1.90 | 3.08 | -6.46 | -1.97 | 9.01 | 1.40 | 2.62 | -5.45 |
Cytoplasm | 0.17 | 5.89 | 3.85 | 4.10 | -2.56 | -10.87 | -3.15 | -8.70 | 1.01 | -6.54 | -9.10 | 0.38 | -5.49 | 2.84 | -5.70 |
Cytoplasmic Foci | 9.41 | 18.68 | 19.57 | 13.64 | -0.79 | -2.82 | 20.10 | 14.75 | 17.25 | -6.39 | 2.83 | 28.72 | 22.56 | 21.92 | -7.72 |
Eisosomes | 6.88 | 1.17 | 9.51 | 4.26 | 4.47 | 6.18 | 12.66 | 9.50 | 4.54 | -4.26 | 8.73 | 11.90 | 13.21 | 6.16 | 0.74 |
Endoplasmic Reticulum | 4.10 | 2.53 | 6.13 | 5.32 | 3.64 | -0.34 | 6.36 | 6.28 | 1.59 | 0.02 | 0.65 | 7.57 | 8.75 | 2.18 | 2.17 |
Endosome | 8.58 | 13.56 | 13.54 | 10.40 | -0.01 | 2.13 | 15.35 | 10.96 | 8.51 | -5.22 | 7.27 | 20.23 | 17.46 | 13.01 | -5.58 |
Golgi | 4.93 | 11.82 | 11.31 | 6.99 | 0.30 | -2.74 | 12.68 | 0.18 | 1.70 | -3.40 | 0.52 | 17.31 | 5.16 | 4.39 | -3.72 |
Lipid Particles | 6.33 | 9.46 | 7.29 | 1.85 | -4.76 | 4.03 | 10.95 | 6.86 | 3.65 | -6.84 | 6.86 | 14.37 | 9.36 | 3.67 | -8.96 |
Mitochondria | -0.03 | 1.90 | 1.79 | 1.02 | -0.00 | -0.88 | 2.70 | 1.45 | 2.04 | -3.18 | -0.64 | 3.33 | 2.11 | 2.20 | -3.18 |
Mitotic Spindle | -11.46 | -19.11 | -23.15 | -14.23 | -0.77 | 3.23 | -19.25 | -7.46 | -10.39 | 9.27 | -3.16 | -28.76 | -18.91 | -16.99 | 8.62 |
None | 0.95 | 1.93 | 2.26 | 1.67 | -0.07 | -4.57 | -1.47 | -1.82 | 2.09 | -1.45 | -4.09 | 1.05 | -0.92 | 2.46 | -1.45 |
Nuclear Periphery | 1.14 | -5.84 | -3.24 | -4.43 | 3.77 | 0.32 | -1.93 | -2.08 | -1.47 | -0.90 | 0.65 | -4.01 | -2.70 | -2.28 | 0.43 |
Nuclear Periphery Foci | 4.93 | 13.21 | 13.91 | 12.66 | 0.87 | 9.62 | 17.04 | 17.06 | 10.84 | 0.41 | 9.36 | 21.94 | 21.79 | 16.51 | -0.62 |
Nucleolus | 2.32 | -2.35 | 3.14 | 1.58 | 4.02 | 2.34 | 3.01 | 3.59 | 1.77 | 1.08 | 3.25 | 1.61 | 4.74 | 2.38 | 3.04 |
Nucleus | 0.97 | -3.01 | -0.05 | -1.10 | 3.01 | -3.50 | -6.76 | -5.82 | -3.62 | 0.53 | -1.92 | -6.87 | -4.94 | -3.62 | 2.59 |
Peroxisomes | 5.68 | 3.50 | 8.87 | 5.16 | 4.96 | 4.05 | 10.98 | 6.76 | 3.47 | -3.36 | 6.50 | 12.65 | 10.96 | 5.85 | -1.35 |
Vacuole | -0.79 | -3.22 | -4.18 | -3.96 | 0.62 | -2.18 | -5.15 | -7.75 | -7.10 | -4.51 | -2.41 | -5.94 | -8.83 | -8.10 | -3.77 |
Vacuole Periphery | 1.26 | 2.77 | 3.13 | 2.26 | 1.00 | -3.09 | 4.09 | -2.07 | 0.06 | -3.16 | -1.81 | 5.13 | -0.55 | 0.84 | -3.29 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|