Standard name
Human Ortholog
Description 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase; catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0 0.05 0.06 0.05 0.06 0 0 0.05 0 0.08 0.07 0.08 0.09 0.27 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.06 0.1 0.16 0.23 0.18 0.2 0 0.07 0 0.12 0.16 0.11 0 0 0 0 0 0
Nucleus 0.97 0.97 0.9 0.86 0.82 0.77 0.8 0.77 0.72 0.71 0.68 0.67 0.87 0.82 0.8 0.77 0.73 0.79 0.95 0.97 0.87 0.8 0.68 0.52
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0.11 0.12 0.14 0.21 0.29 0.29 0.33 0.44 0.46 0.41 0.51 0.21 0.24 0.27 0.14 0.07 0.08 0 0 0 0.09 0.07 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.2 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0 2 0 2 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 8 1 6 6 3 7 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 0 10 6 9 13 16 22 13 6 10 7 8 11 18 18 35 18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 3 1 1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 1 0 0 0 1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0
Mitochondria 0 0 1 7 7 28 20 37 47 74 38 53 3 11 10 26 20 17 0 0 0 0 1 0
Nucleus 239 117 155 143 138 176 267 300 216 229 146 179 87 121 182 160 94 117 235 119 148 69 109 84
Nuclear Periphery 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 25 13 20 24 35 66 96 127 133 150 87 136 21 35 62 29 9 12 4 0 7 7 11 22
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 1 2 1 0 2 2 1 1 0 1 0 0 3 5 5 6 3 1 6 5 32 46
Unique Cell Count 246 120 172 166 169 228 335 388 301 323 214 266 100 148 227 209 129 149 249 124 171 87 160 163
Labelled Cell Count 267 130 187 183 190 283 414 489 418 467 284 383 121 181 280 242 167 172 249 124 171 87 160 163


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 40.8 49.1 39.1 37.3 36.0 32.6 31.5 34.6 32.8 29.9 32.0 30.7 53.4 56.0 55.8 76.0 86.7 96.2 37.5 47.6 52.2
Std Deviation (1e-4) 11.9 16.3 18.2 15.4 15.1 15.6 16.3 16.6 13.9 12.8 16.0 12.9 21.4 23.4 27.8 38.2 50.8 47.9 15.4 21.5 22.5
Intensity Change (Log2) – – – -0.07 -0.12 -0.27 -0.31 -0.18 -0.26 -0.39 -0.29 -0.35 0.45 0.52 0.51 0.96 1.15 1.3 -0.06 0.28 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -0.2 0 -0.5 -0.1 -0.7 -2.4 -0.5 -1.7 0.7 0.6 0.8 1.0 5.1 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.5 2.9 3.9 5.2 6.6 5.6 6.0 0 3.2 0 4.5 5.0 4.2
Nucleus -1.1 -2.2 -3.4 -3.0 -3.6 -4.7 -4.9 -5.2 -5.5 -0.8 -2.2 -2.7 -3.5 -3.9 -2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 2.3 4.2 4.3 5.2 7.3 7.8 6.3 8.4 2.1 2.8 3.8 0.7 -1.4 -1.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 36.0821 41.1731 37.3626 39.3216 35.021 36.2594 37.3898 37.4083 36.2736 33.3979 25.0846 34.291 37.1771 43.7499 41.182 40.6073 38.5456 37.6662
Actin 0.0024 0.0018 0 0 0.0005 0.0009 0.0008 0 0.0004 0 0.0312 0.0005 0.0113 0 0.0028 0 0 0
Bud 0.0003 0.0013 0.0001 0 0.0001 0.0002 0.0003 0 0.0001 0 0.0006 0.0001 0.0003 0 0 0 0 0.0001
Bud Neck 0.0004 0.0001 0.0001 0 0.0019 0.0002 0.0001 0 0.0001 0 0.0003 0.0001 0.0019 0 0.0001 0 0 0.0001
Bud Periphery 0.0003 0.0005 0.0001 0 0.0006 0.0002 0.0002 0 0.0001 0 0.0006 0.0001 0.0004 0 0 0 0 0.0001
Bud Site 0.0005 0.002 0 0 0.0001 0.0001 0.0004 0 0.0004 0 0.0035 0.0001 0.0023 0 0.0001 0 0 0.0001
Cell Periphery 0.0005 0.0007 0.0001 0 0.0007 0.0007 0.0007 0 0.0002 0 0.0003 0.0001 0.0003 0 0.0001 0 0.0001 0.0001
Cytoplasm 0.0005 0.003 0.0001 0.0003 0.0004 0.0014 0.0033 0 0.0004 0 0.0012 0 0.001 0.0002 0.0006 0 0 0.0002
Cytoplasmic Foci 0.0153 0.0096 0.0039 0.0015 0.0132 0.0017 0.0035 0 0.0031 0 0.03 0.0004 0.006 0.003 0.0042 0 0 0.0009
Eisosomes 0.0001 0.0002 0 0 0.0001 0 0 0 0 0 0.0004 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0003 0.0007 0 0 0.0002 0.0001 0.0003 0 0.0001 0 0.0007 0.0001 0.0005 0 0.0008 0 0 0
Endosome 0.0048 0.0011 0.0001 0.0002 0.0098 0.0026 0.0032 0 0.0033 0 0.028 0.0029 0.0076 0 0.0023 0 0 0
Golgi 0.0037 0.0006 0 0 0.0045 0.0002 0.0004 0 0.0014 0 0.0325 0.0016 0.0021 0 0.0004 0 0 0
Lipid Particles 0.0078 0.001 0.0015 0.0002 0.0158 0.0006 0.0021 0 0.0019 0 0.0169 0.0003 0.0042 0.0004 0.0014 0 0 0.0003
Mitochondria 0.0051 0.0119 0.0022 0.0002 0.033 0.0047 0.0031 0.0004 0.0056 0.0002 0.0332 0.0016 0.0045 0.0008 0.0014 0.0002 0.0026 0.0022
None 0.0026 0.0023 0.0002 0.0002 0.0004 0.0004 0.0026 0 0.0003 0 0.0016 0 0.0031 0.0006 0.001 0 0 0.0002
Nuclear Periphery 0.0012 0.0006 0.0005 0.0018 0.0026 0.0004 0.0033 0.0037 0.0006 0.0001 0.0018 0.0004 0.0029 0.0032 0.0035 0.0001 0.0004 0.0001
Nucleolus 0.0837 0.0472 0.033 0.0183 0.1712 0.1109 0.0634 0.0171 0.0228 0.0071 0.1679 0.0721 0.0844 0.0243 0.0175 0.0051 0.1193 0.0676
Nucleus 0.859 0.9119 0.9567 0.9423 0.7328 0.8707 0.9011 0.9782 0.9572 0.9926 0.598 0.9188 0.8528 0.9659 0.9617 0.9947 0.8768 0.9268
Peroxisomes 0.0057 0.0001 0.0002 0 0.0005 0.0001 0.0004 0 0.001 0 0.0429 0.0001 0.0041 0 0.0002 0 0 0.0001
Punctate Nuclear 0.0035 0.0008 0.0002 0.0348 0.0012 0.0001 0.005 0 0.0002 0 0.0053 0.0001 0.0086 0.0009 0.0014 0 0.0001 0.0001
Vacuole 0.0011 0.0015 0.0004 0 0.0037 0.0024 0.0027 0.0001 0.0003 0 0.001 0.0003 0.0007 0.0001 0.0002 0 0.0001 0.0004
Vacuole Periphery 0.0014 0.001 0.0007 0.0001 0.0067 0.0016 0.0032 0.0005 0.0006 0 0.0022 0.0003 0.001 0.0004 0.0003 0 0.0005 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 337.5251 298.0513 291.5833 344.2054 310.6444 238.3409 319.7721 296.431 276.1417 356.625
Translational Efficiency 1.6545 1.8226 1.7709 1.4004 1.6927 2.064 1.7274 1.5005 1.7087 1.5437

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
128 1587 1896 1035 1412 1299 1228 2912 1540 2886 3124 3947

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2275.38 3300.25 6083.12 5922.16 3431.41 3005.26 4674.33 5832.03 3335.32 3167.47 5529.34 5855.66
Standard Deviation 690.51 1146.95 1934.04 2300.76 1185.64 966.79 1162.18 2547.47 1195.98 1079.64 1809.58 2485.46
Intensity Change Log 2 — 0.536468 1.418704 1.380016 — -0.191312 0.445958 0.765197 — 0.143934 0.914585 1.042424

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000305 0.000446 0.000449 0.000686 0.000475 0.000545 0.000517 0.000460 0.000461 0.000490 0.000476 0.000519
Bud Neck 0.000927 0.000923 0.002526 0.003055 0.000875 0.001154 0.002762 0.002368 0.000880 0.001027 0.002619 0.002548
Bud Site 0.000156 0.000189 0.000299 0.000297 0.000092 0.000211 0.000240 0.000522 0.000097 0.000199 0.000276 0.000463
Cell Periphery 0.000017 0.000457 0.000144 0.000092 0.000396 0.000225 0.000087 0.000405 0.000365 0.000353 0.000121 0.000323
Cytoplasm 0.000026 0.000273 0.000071 0.000348 0.000125 0.000250 0.000291 0.002881 0.000117 0.000262 0.000158 0.002217
Cytoplasmic Foci 0.000109 0.000307 0.000420 0.001531 0.000083 0.000304 0.000219 0.000333 0.000085 0.000306 0.000341 0.000647
Eisosomes 0.000158 0.000431 0.000337 0.000118 0.001352 0.000354 0.000421 0.000192 0.001253 0.000397 0.000370 0.000172
Endoplasmic Reticulum 0.000095 0.000189 0.000145 0.000120 0.000312 0.000287 0.000181 0.000258 0.000294 0.000234 0.000159 0.000222
Endosome 0.000002 0.000004 0.000003 0.000056 0.000002 0.000018 0.000027 0.000009 0.000002 0.000011 0.000012 0.000021
Golgi 0.000004 0.000007 0.000009 0.000038 0.000002 0.000022 0.000007 0.000016 0.000003 0.000014 0.000008 0.000022
Lipid Particles 0.000001 0.000002 0.000001 0.000454 0.000000 0.000026 0.000004 0.000002 0.000000 0.000013 0.000002 0.000120
Mitochondria 0.000540 0.000670 0.000933 0.002189 0.000514 0.001012 0.001151 0.000986 0.000516 0.000824 0.001019 0.001301
Mitotic Spindle 0.000000 0.000005 0.000022 0.000478 0.000001 0.001385 0.000004 0.000349 0.000001 0.000626 0.000015 0.000383
None 0.000467 0.001481 0.000434 0.002209 0.000972 0.000962 0.000275 0.004726 0.000930 0.001247 0.000372 0.004066
Nuclear Periphery 0.000006 0.000019 0.000031 0.000980 0.000022 0.000024 0.000018 0.000160 0.000021 0.000021 0.000026 0.000375
Nuclear Periphery Foci 0.000000 0.000002 0.000001 0.000031 0.000000 0.000021 0.000004 0.000028 0.000000 0.000010 0.000002 0.000029
Nucleolus 0.005940 0.006011 0.005544 0.017244 0.004082 0.007816 0.007386 0.003803 0.004237 0.006824 0.006268 0.007328
Nucleus 0.989672* 0.985861* 0.986659* 0.966643* 0.988673* 0.983186* 0.983784* 0.980932* 0.988756* 0.984657* 0.985529* 0.977185*
Peroxisomes 0.001436 0.000772 0.001616 0.001907 0.000341 0.001438 0.001946 0.000280 0.000432 0.001072 0.001746 0.000707
Vacuole 0.000101 0.001487 0.000270 0.000412 0.001444 0.000595 0.000580 0.000890 0.001333 0.001086 0.000392 0.000765
Vacuole Periphery 0.000039 0.000465 0.000085 0.001111 0.000236 0.000165 0.000096 0.000401 0.000220 0.000330 0.000089 0.000587

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.31 -4.97 -6.36 -4.35 -4.31 -0.58 -1.94 0.43 0.66 2.17 -0.53 -1.06 -2.40 -0.48 -1.89
Bud Neck -0.02 -11.00 -6.48 -6.76 -1.65 -2.77 -14.05 -9.37 -6.61 1.52 -2.22 -19.29 -11.41 -10.17 0.04
Bud Site -0.46 -2.77 -2.56 -1.09 -0.24 -1.69 -8.61 -3.37 -1.61 -2.22 -1.92 -9.73 -3.88 -2.06 -2.02
Cell Periphery -2.04 -3.70 -3.62 1.67 1.23 1.18 4.57 -0.17 -1.25 -4.19 0.05 3.98 0.45 0.20 -3.54
Cytoplasm -1.68 -4.92 -4.91 0.14 -4.30 -1.32 -0.75 -5.08 -4.73 -3.91 -1.70 -0.58 -5.25 -4.45 -4.81
Cytoplasmic Foci -1.50 -3.47 -2.57 -2.19 -2.02 -1.98 -2.88 -3.18 -0.10 -1.45 -2.54 -4.43 -3.66 -1.94 -2.03
Eisosomes -5.00 -3.94 1.15 7.11 6.83 2.94 2.74 3.43 2.62 3.79 2.76 2.85 3.49 5.15 5.04
Endoplasmic Reticulum -3.85 -3.47 -1.45 2.65 1.47 0.19 3.53 1.44 0.31 -2.44 1.04 4.56 2.21 0.25 -3.14
Endosome -1.55 -1.92 -1.07 -1.02 -1.05 -2.18 -0.99 -4.89 1.28 0.77 -2.49 -1.03 -1.51 -0.70 -0.27
Golgi -1.54 -5.00 -3.29 -2.97 -2.78 -2.88 -7.11 -4.66 0.72 -3.24 -3.38 -8.55 -5.62 -1.75 -4.02
Lipid Particles -1.03 0.89 -1.00 -1.00 -1.00 -1.40 -1.20 -2.14 1.30 0.65 -1.51 -1.34 -1.02 -0.90 -1.00
Mitochondria -2.17 -6.58 -8.78 -8.32 -6.78 -1.83 -11.41 -9.46 0.10 2.48 -2.45 -13.86 -12.63 -3.49 -4.31
Mitotic Spindle -1.55 -1.69 -1.26 -1.25 -1.21 -1.62 -1.23 -1.95 1.07 -1.94 -1.63 -1.78 -2.33 0.40 -2.26
None -1.68 0.38 -1.93 -0.66 -1.96 -0.00 2.04 -4.54 -4.60 -5.87 -0.68 1.78 -4.61 -4.00 -6.07
Nuclear Periphery -5.11 -7.55 -1.43 -1.41 -1.39 -0.71 1.56 -2.82 -2.74 -2.88 -0.37 -2.33 -1.96 -1.96 -1.93
Nuclear Periphery Foci -2.09 -2.37 -2.12 -1.99 -2.08 -2.31 -1.01 -2.47 -0.56 -1.94 -2.50 -1.15 -3.04 -1.77 -2.76
Nucleolus -0.06 0.23 -4.69 -5.10 -5.23 -3.17 -2.61 0.15 3.57 2.93 -2.99 -2.32 -3.32 -0.71 -1.26
Nucleus 1.98 1.79 6.44 5.46 5.91 2.77 2.89 4.76 1.09 1.63 2.97 2.73 7.25 4.45 5.57
Peroxisomes 2.40 -0.62 -1.32 -3.99 -0.85 -5.39 -7.58 0.68 5.77 8.00 -5.45 -8.27 -2.99 2.74 6.16
Vacuole -3.90 -4.94 -3.38 2.92 -1.54 3.08 2.04 1.82 -1.49 -0.72 0.75 3.55 2.14 1.36 -2.07
Vacuole Periphery -2.54 -6.36 -2.18 -1.25 -2.09 1.31 2.73 -0.85 -1.26 -1.65 -1.07 2.83 -1.91 -1.23 -2.66
Standard name
Human Ortholog
Description 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase; catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-1

Erg13

Erg13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erg13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available