Standard name
Human Ortholog
Description Polo-like kinase; controls targeting and activation of Rho1p at cell division site via Rho1p guanine nucleotide exchange factors; regulates Spc72p; also functions in adaptation to DNA damage during meiosis; regulates the shape of the nucleus and expansion of the nuclear envelope during mitosis; similar to Xenopus Plx1 and S. pombe Plo1p; human homologs PLK1, PLK3 can each complement yeast cdc5 thermosensitive mutants
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0.05 0.05 0 0 0
Bud Neck 0 0 0.05 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.64 0.66 0.75 0.82 0.66 0.74 0.66 0.73 0.61 0.63 0.55 0.58 0.78 0.79 0.74 0.58 0.6 – 0.32 0.39 0.36 0.65 0.68 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0.12 0.11 0.12 0.15 – 0.08 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.05 0.12 0 0 0 0.13 0.18 0.09 0.34 0.32 0.35 0.24 0.06 0 0 0.05 0 – 0.11 0.06 0.1 0.15 0.16 0.11
Nucleus 0.05 0.09 0.07 0 0.05 0.06 0.1 0.08 0.09 0.1 0.09 0.11 0 0 0.06 0 0 – 0 0.09 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0
Nucleolus 0.1 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0.05 0 0.06 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0.09 0 0.07 0.08 0.2 0.06 0.08 0.07 0.05 0.05 0 0.08 0 0 0 0 0.11 – 0.21 0.2 0.21 0 0 0
Vac/Vac Membrane 0.09 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0.22 0.14 – 0.05 0.06 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 7 2 7 0 3 2 1 2 1 1 2 0 0 0 1 – 0 2 4 2 6 4
Bud 0 0 3 5 15 7 13 15 18 17 22 13 3 1 0 0 0 – 10 14 23 4 6 5
Bud Neck 0 0 18 12 29 14 22 29 15 13 10 14 0 1 1 0 0 – 1 8 15 5 4 7
Bud Site 0 1 1 3 1 1 2 1 5 6 8 9 2 1 1 0 0 – – – – – – –
Cell Periphery 1 0 3 0 1 1 0 2 0 2 4 0 0 1 0 2 0 – 0 0 3 0 5 5
Cytoplasm 105 194 286 302 303 334 327 391 307 417 322 328 265 388 333 57 59 – 63 129 155 126 293 270
Endoplasmic Reticulum 3 4 8 1 3 4 1 0 0 0 0 0 0 0 2 4 5 – 1 0 2 1 8 2
Endosome 12 12 3 1 2 2 0 2 1 2 5 6 18 61 50 12 15 – 16 16 17 3 1 3
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 2 2 – 2 2 0 0 1 0
Mitochondria 8 34 1 13 10 58 91 47 169 212 206 137 21 10 16 5 4 – 22 18 42 28 67 38
Nucleus 8 25 26 14 24 27 51 44 45 64 54 64 9 10 25 3 2 – 7 28 15 1 0 2
Nuclear Periphery 3 2 3 6 1 8 6 3 10 11 5 5 4 6 2 0 1 – 10 10 14 3 2 1
Nucleolus 17 5 3 6 13 9 9 2 16 16 31 17 17 27 21 3 6 – 2 4 3 1 0 0
Peroxisomes 1 0 0 0 0 0 0 0 1 1 0 2 2 1 4 0 0 – 1 0 1 0 0 0
SpindlePole 15 11 26 30 90 26 37 38 27 33 23 48 15 12 13 2 11 – 42 64 90 5 4 2
Vac/Vac Membrane 15 29 13 7 11 10 5 17 5 8 14 16 7 14 14 22 14 – 10 18 24 8 16 10
Unique Cell Count 163 293 382 370 456 452 493 535 502 664 583 570 340 493 453 98 98 199 328 434 196 433 358
Labelled Cell Count 189 318 401 403 510 501 567 593 620 804 705 660 365 534 482 112 120 199 328 434 196 433 358


Bud Neck, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 3.9 4.7 4.3 4.9 3.7 3.8 4.2 3.4 3.3 3.3 3.8 3.9 4.0 4.2 6.4 6.2 – 3.7 3.5 3.8
Std Deviation (1e-4) 1.0 1.0 0.9 1.1 1.2 1.0 1.0 1.1 1.1 0.9 1.0 1.0 1.0 1.2 1.5 2.0 1.7 – 1.2 1.3 0.9
Intensity Change (Log2) – – – -0.15 0.06 -0.36 -0.3 -0.19 -0.49 -0.54 -0.5 -0.31 -0.27 -0.24 -0.17 0.43 0.4 – -0.37 -0.42 -0.32

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_202468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 6.5 4.2 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.569 -0.2468 0.0053 1.3495 1.0737 -0.4586 -0.6765 -0.3299 0.0187 0.9773 1.1029 -0.5358 -0.869 0.1332 0.0899 1.1084 1.7813 -0.4957
Actin 0.012 0.0008 0.0055 0.0134 0.0054 0.0018 0.0386 0.0047 0.0157 0.0017 0.004 0.0066 0.0278 0.0018 0.0213 0.0386 0.0114 0.0052
Bud 0.0015 0.0037 0.0021 0.0024 0.0006 0.0009 0.0021 0.0034 0.0009 0.0002 0.0004 0.0015 0.0033 0.0041 0.0005 0.0005 0.0007 0.0009
Bud Neck 0.017 0.0069 0.0058 0.0272 0.0693 0.0826 0.0293 0.0015 0.0098 0.0163 0.0898 0.1015 0.006 0.0007 0.0013 0.0019 0.1315 0.1484
Bud Periphery 0.0039 0.0037 0.0024 0.0027 0.0017 0.0012 0.0027 0.0034 0.0014 0.0009 0.0013 0.0031 0.0076 0.0043 0.0009 0.001 0.0023 0.0025
Bud Site 0.0067 0.0168 0.0209 0.0091 0.0018 0.0019 0.033 0.029 0.0049 0.0003 0.0016 0.0004 0.0087 0.0078 0.0161 0.0022 0.0023 0.0014
Cell Periphery 0.0006 0.0004 0.0004 0.0003 0.0003 0.0002 0.0007 0.0004 0.0002 0.0001 0.0003 0.0002 0.0006 0.0003 0.0002 0.0002 0.0006 0.0003
Cytoplasm 0.0662 0.0744 0.0365 0.0401 0.0444 0.0974 0.0819 0.09 0.0241 0.008 0.0325 0.2011 0.0501 0.1141 0.0355 0.0047 0.0081 0.1327
Cytoplasmic Foci 0.0644 0.0191 0.0504 0.007 0.0127 0.0329 0.0823 0.0452 0.1012 0.0021 0.0206 0.0266 0.1112 0.0353 0.062 0.0039 0.0216 0.0328
Eisosomes 0.0003 0.0001 0.0002 0 0.0001 0.0001 0.0006 0.0001 0.0002 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0096 0.0057 0.0036 0.0047 0.0049 0.0047 0.0063 0.0026 0.0029 0.0029 0.0075 0.0048 0.0075 0.0045 0.0022 0.0032 0.0057 0.0032
Endosome 0.0296 0.0064 0.0177 0.0113 0.018 0.0098 0.0282 0.0047 0.0283 0.0051 0.0666 0.0096 0.0403 0.0143 0.0149 0.0064 0.0426 0.012
Golgi 0.0028 0.0004 0.0042 0.0044 0.0068 0.0011 0.0145 0.0012 0.0121 0.0002 0.0067 0.001 0.0065 0.0075 0.0066 0.0059 0.0095 0.0024
Lipid Particles 0.0198 0.0035 0.0163 0.0048 0.0399 0.0059 0.05 0.0056 0.0239 0.0025 0.0229 0.0046 0.0552 0.0059 0.0287 0.0173 0.052 0.0077
Mitochondria 0.0061 0.0013 0.0035 0.002 0.012 0.0015 0.0095 0.0035 0.0165 0.0018 0.0036 0.0013 0.0131 0.0031 0.0043 0.0049 0.0182 0.0013
None 0.556 0.548 0.2642 0.008 0.0427 0.5885 0.4386 0.499 0.1357 0.0049 0.0049 0.5109 0.4173 0.4429 0.211 0.0026 0.0034 0.5522
Nuclear Periphery 0.0686 0.0609 0.1409 0.2659 0.2529 0.0305 0.047 0.0762 0.1182 0.3741 0.2981 0.0329 0.0692 0.0744 0.1526 0.2888 0.367 0.0249
Nucleolus 0.0157 0.0088 0.0185 0.0106 0.0295 0.0081 0.0071 0.0066 0.0086 0.0108 0.0253 0.003 0.0078 0.0066 0.0099 0.0087 0.037 0.0031
Nucleus 0.0545 0.1573 0.1886 0.4433 0.2816 0.0619 0.0229 0.136 0.1246 0.5045 0.2497 0.0378 0.0233 0.1329 0.1418 0.4609 0.128 0.028
Peroxisomes 0.0067 0.0015 0.003 0.0014 0.0249 0.0012 0.025 0.0029 0.0154 0.0002 0.0027 0.0032 0.0326 0.0011 0.0087 0.0083 0.0206 0.0028
Punctate Nuclear 0.034 0.0723 0.2072 0.1359 0.1374 0.0617 0.0695 0.0785 0.3486 0.0502 0.144 0.0418 0.0939 0.1321 0.2756 0.1331 0.1155 0.0338
Vacuole 0.0159 0.006 0.0043 0.0024 0.0053 0.0046 0.0068 0.0035 0.0035 0.0041 0.009 0.0056 0.0111 0.0039 0.0023 0.0017 0.008 0.0029
Vacuole Periphery 0.0081 0.002 0.0039 0.003 0.0077 0.0018 0.0035 0.002 0.0033 0.0092 0.0083 0.0024 0.0065 0.0024 0.0034 0.0052 0.0138 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.075 53.0911 50.4544 40.0409 19.5882 8.8515 57.5651 72.9379 44.9577 22.6008
Translational Efficiency 1.0927 0.9682 0.9339 0.7645 0.6557 0.8499 0.909 0.693 0.8939 0.7558

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
300 726 223 888 2026 1284 128 96 2326 2010 351 984

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 655.95 748.17 1002.89 912.35 719.27 726.38 945.67 974.68 711.10 734.25 982.02 918.43
Standard Deviation 74.76 94.08 120.34 144.66 83.64 96.80 95.69 154.00 85.23 96.40 115.32 146.77
Intensity Change Log 2 — 0.189780 0.612506 0.476002 — 0.014191 0.394803 0.438395 — 0.100612 0.502746 0.456455

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000127 0.000547 0.001334 0.005485 0.000187 0.000666 0.001003 0.001591 0.000180 0.000623 0.001213 0.005105
Bud Neck 0.034943 0.033885 0.010913 0.042387 0.027308 0.050731 0.030684 0.055243 0.028292 0.044646 0.018123 0.043641
Bud Site 0.002641 0.011534 0.009785 0.070533 0.006177 0.018428 0.019241 0.085861 0.005721 0.015938 0.013233 0.072028
Cell Periphery 0.000311 0.000188 0.000239 0.000481 0.000212 0.000256 0.000328 0.000349 0.000225 0.000231 0.000271 0.000468
Cytoplasm 0.351682 0.304477 0.100116 0.134020 0.435305 0.260037 0.257449 0.074286 0.424520 0.276088 0.157491 0.128193
Cytoplasmic Foci 0.235877 0.204246 0.005249 0.034626 0.185954 0.232143 0.030077 0.031254 0.192393 0.222067 0.014303 0.034297
Eisosomes 0.000231 0.000128 0.000085 0.000106 0.000131 0.000188 0.000152 0.000073 0.000144 0.000167 0.000110 0.000103
Endoplasmic Reticulum 0.001938 0.004192 0.022269 0.017368 0.002037 0.003106 0.010327 0.004032 0.002024 0.003498 0.017914 0.016067
Endosome 0.013120 0.019155 0.004300 0.035450 0.012712 0.032836 0.009731 0.008749 0.012765 0.027894 0.006280 0.032845
Golgi 0.002378 0.003443 0.000222 0.019778 0.003410 0.009242 0.000393 0.036403 0.003277 0.007148 0.000284 0.021400
Lipid Particles 0.044084 0.019472 0.002657 0.003895 0.021879 0.043072 0.007255 0.002130 0.024743 0.034548 0.004334 0.003723
Mitochondria 0.013784 0.011850 0.001080 0.006878 0.007363 0.018811 0.000650 0.001545 0.008191 0.016297 0.000923 0.006358
Mitotic Spindle 0.006782 0.039876 0.019756 0.193585 0.010357 0.046542 0.009785 0.256285 0.009896 0.044134 0.016120 0.199702
None 0.020430 0.011285 0.003260 0.009488 0.017617 0.013460 0.007455 0.017115 0.017980 0.012675 0.004790 0.010232
Nuclear Periphery 0.011969 0.007901 0.005643 0.006730 0.003747 0.005307 0.002861 0.004681 0.004807 0.006244 0.004629 0.006530
Nuclear Periphery Foci 0.032233 0.056140 0.004043 0.019299 0.019562 0.041570 0.007398 0.054615 0.021197 0.046833 0.005267 0.022745
Nucleolus 0.025885 0.025341 0.004373 0.007151 0.016815 0.026402 0.003731 0.030664 0.017985 0.026018 0.004139 0.009445
Nucleus 0.169633 0.188326 0.741699 0.258086 0.196736 0.119225 0.479015 0.261612 0.193240 0.144184 0.645905 0.258430
Peroxisomes 0.012891 0.021001 0.000652 0.015234 0.013272 0.043106 0.019033 0.018593 0.013222 0.035122 0.007355 0.015562
Vacuole 0.018157 0.035602 0.061939 0.105921 0.018452 0.032238 0.103112 0.048735 0.018414 0.033453 0.076954 0.100342
Vacuole Periphery 0.000904 0.001412 0.000388 0.013497 0.000770 0.002632 0.000318 0.006185 0.000787 0.002191 0.000362 0.012784

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.71 -9.41 -4.25 -3.60 -1.65 -7.15 -5.11 -7.28 -5.10 -0.37 -8.92 -10.32 -4.47 -3.75 -1.76
Bud Neck 0.11 2.13 -0.30 -0.69 -4.55 -4.78 0.78 -1.74 0.54 -1.90 -4.12 2.45 -2.29 1.76 -4.18
Bud Site -5.36 -3.01 -10.00 -7.21 -5.40 -6.07 -2.26 -3.46 -2.51 -2.28 -6.95 -3.13 -9.80 -6.80 -5.68
Cell Periphery 3.50 0.43 0.52 -3.30 0.11 -2.35 -4.33 -1.62 -1.11 0.24 -0.25 -4.14 -2.43 -2.30 0.80
Cytoplasm 1.95 12.82 11.18 12.71 -3.67 16.21 9.73 25.23 12.35 5.18 15.84 23.39 31.75 15.90 0.56
Cytoplasmic Foci 2.56 17.00 16.10 21.27 -6.42 -4.71 24.44 24.59 25.57 1.37 -2.83 37.37 37.72 37.64 -1.64
Eisosomes 4.76 4.79 5.03 0.71 -0.21 -4.99 -3.37 0.74 3.50 3.34 -2.42 -1.00 2.77 5.36 2.77
Endoplasmic Reticulum -3.05 -9.07 -8.36 -6.80 3.21 -2.27 -7.77 -5.04 -4.40 2.68 -3.72 -11.56 -9.13 -8.00 3.32
Endosome -2.72 4.10 -1.03 1.79 -6.15 -9.49 1.73 3.33 10.46 1.11 -9.86 6.26 -1.29 6.24 -5.01
Golgi -1.30 6.23 -1.73 -1.22 -3.36 -5.07 5.30 -0.61 0.14 -1.03 -4.60 6.03 -1.45 0.77 -3.44
Lipid Particles 3.87 6.40 6.36 6.64 -0.80 -6.19 7.64 11.42 13.07 2.09 -3.19 13.12 14.69 15.10 1.85
Mitochondria 0.44 2.63 2.06 3.25 -6.16 -6.03 7.68 5.68 10.40 -4.16 -4.58 7.42 4.45 9.19 -6.97
Mitotic Spindle -6.30 -2.33 -15.67 -10.22 -10.21 -8.99 2.16 -6.29 -5.11 -6.48 -11.02 -1.82 -17.48 -12.41 -13.78
None 2.52 5.43 4.00 3.19 -5.37 2.36 8.04 1.74 0.31 -3.17 3.74 12.95 8.60 4.89 -4.95
Nuclear Periphery 0.79 1.35 0.45 -1.06 -4.22 -1.41 -0.21 -3.01 -1.57 -3.05 -1.15 0.15 -4.40 -3.15 -5.60
Nuclear Periphery Foci -1.32 5.09 1.87 4.15 -7.72 -4.91 2.70 -0.92 0.97 -1.83 -5.93 7.15 -0.98 4.54 -6.84
Nucleolus 0.84 5.78 3.65 4.54 -5.76 -2.66 9.06 1.81 3.30 -2.77 -2.51 11.62 4.11 6.28 -6.58
Nucleus -1.83 -25.01 -10.38 -9.66 17.55 10.28 -10.44 -4.09 -6.23 3.37 6.93 -25.54 -11.96 -15.89 15.25
Peroxisomes -1.82 5.97 3.30 6.61 -4.96 -9.99 0.33 3.75 11.07 1.24 -9.90 4.23 5.31 13.37 -0.68
Vacuole -4.90 -7.79 -17.52 -15.19 -5.18 -5.77 -8.99 -4.53 -4.02 1.50 -8.01 -11.68 -18.72 -16.52 -2.76
Vacuole Periphery -2.59 2.95 -2.53 -2.14 -2.90 -4.88 6.10 -0.71 0.66 -1.01 -5.57 6.30 -2.68 -1.47 -3.00
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Polo-like kinase; controls targeting and activation of Rho1p at cell division site via Rho1p guanine nucleotide exchange factors; regulates Spc72p; also functions in adaptation to DNA damage during meiosis; regulates the shape of the nucleus and expansion of the nuclear envelope during mitosis; similar to Xenopus Plx1 and S. pombe Plo1p; human homologs PLK1, PLK3 can each complement yeast cdc5 thermosensitive mutants
Localization
Cell Percentages cytoplasm (20%), nucleus (10%), mixed (20%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (1.3e-13)
nucleus - S/G2 (1.3e-07)
nucleus - MA (3.2e-06)
Subcompartmental Group N/A

Cdc5

Cdc5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available