Standard name | |
---|---|
Human Ortholog | |
Description | Polo-like kinase; controls targeting and activation of Rho1p at cell division site via Rho1p guanine nucleotide exchange factors; regulates Spc72p; also functions in adaptation to DNA damage during meiosis; regulates the shape of the nucleus and expansion of the nuclear envelope during mitosis; similar to Xenopus Plx1 and S. pombe Plo1p; human homologs PLK1, PLK3 can each complement yeast cdc5 thermosensitive mutants |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 7 | 2 | 7 | 0 | 3 | 2 | 1 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | – | 0 | 2 | 4 | 2 | 6 | 4 |
Bud | 0 | 0 | 3 | 5 | 15 | 7 | 13 | 15 | 18 | 17 | 22 | 13 | 3 | 1 | 0 | 0 | 0 | – | 10 | 14 | 23 | 4 | 6 | 5 |
Bud Neck | 0 | 0 | 18 | 12 | 29 | 14 | 22 | 29 | 15 | 13 | 10 | 14 | 0 | 1 | 1 | 0 | 0 | – | 1 | 8 | 15 | 5 | 4 | 7 |
Bud Site | 0 | 1 | 1 | 3 | 1 | 1 | 2 | 1 | 5 | 6 | 8 | 9 | 2 | 1 | 1 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 3 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 4 | 0 | 0 | 1 | 0 | 2 | 0 | – | 0 | 0 | 3 | 0 | 5 | 5 |
Cytoplasm | 105 | 194 | 286 | 302 | 303 | 334 | 327 | 391 | 307 | 417 | 322 | 328 | 265 | 388 | 333 | 57 | 59 | – | 63 | 129 | 155 | 126 | 293 | 270 |
Endoplasmic Reticulum | 3 | 4 | 8 | 1 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 5 | – | 1 | 0 | 2 | 1 | 8 | 2 |
Endosome | 12 | 12 | 3 | 1 | 2 | 2 | 0 | 2 | 1 | 2 | 5 | 6 | 18 | 61 | 50 | 12 | 15 | – | 16 | 16 | 17 | 3 | 1 | 3 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | – | 2 | 2 | 0 | 0 | 1 | 0 |
Mitochondria | 8 | 34 | 1 | 13 | 10 | 58 | 91 | 47 | 169 | 212 | 206 | 137 | 21 | 10 | 16 | 5 | 4 | – | 22 | 18 | 42 | 28 | 67 | 38 |
Nucleus | 8 | 25 | 26 | 14 | 24 | 27 | 51 | 44 | 45 | 64 | 54 | 64 | 9 | 10 | 25 | 3 | 2 | – | 7 | 28 | 15 | 1 | 0 | 2 |
Nuclear Periphery | 3 | 2 | 3 | 6 | 1 | 8 | 6 | 3 | 10 | 11 | 5 | 5 | 4 | 6 | 2 | 0 | 1 | – | 10 | 10 | 14 | 3 | 2 | 1 |
Nucleolus | 17 | 5 | 3 | 6 | 13 | 9 | 9 | 2 | 16 | 16 | 31 | 17 | 17 | 27 | 21 | 3 | 6 | – | 2 | 4 | 3 | 1 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 1 | 4 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 15 | 11 | 26 | 30 | 90 | 26 | 37 | 38 | 27 | 33 | 23 | 48 | 15 | 12 | 13 | 2 | 11 | – | 42 | 64 | 90 | 5 | 4 | 2 |
Vac/Vac Membrane | 15 | 29 | 13 | 7 | 11 | 10 | 5 | 17 | 5 | 8 | 14 | 16 | 7 | 14 | 14 | 22 | 14 | – | 10 | 18 | 24 | 8 | 16 | 10 |
Unique Cell Count | 163 | 293 | 382 | 370 | 456 | 452 | 493 | 535 | 502 | 664 | 583 | 570 | 340 | 493 | 453 | 98 | 98 | 199 | 328 | 434 | 196 | 433 | 358 | |
Labelled Cell Count | 189 | 318 | 401 | 403 | 510 | 501 | 567 | 593 | 620 | 804 | 705 | 660 | 365 | 534 | 482 | 112 | 120 | 199 | 328 | 434 | 196 | 433 | 358 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 3.9 | 4.7 | 4.3 | 4.9 | 3.7 | 3.8 | 4.2 | 3.4 | 3.3 | 3.3 | 3.8 | 3.9 | 4.0 | 4.2 | 6.4 | 6.2 | – | 3.7 | 3.5 | 3.8 |
Std Deviation (1e-4) | 1.0 | 1.0 | 0.9 | 1.1 | 1.2 | 1.0 | 1.0 | 1.1 | 1.1 | 0.9 | 1.0 | 1.0 | 1.0 | 1.2 | 1.5 | 2.0 | 1.7 | – | 1.2 | 1.3 | 0.9 |
Intensity Change (Log2) | – | – | – | -0.15 | 0.06 | -0.36 | -0.3 | -0.19 | -0.49 | -0.54 | -0.5 | -0.31 | -0.27 | -0.24 | -0.17 | 0.43 | 0.4 | – | -0.37 | -0.42 | -0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 6.5 | 4.2 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.569 | -0.2468 | 0.0053 | 1.3495 | 1.0737 | -0.4586 | -0.6765 | -0.3299 | 0.0187 | 0.9773 | 1.1029 | -0.5358 | -0.869 | 0.1332 | 0.0899 | 1.1084 | 1.7813 | -0.4957 |
Actin | 0.012 | 0.0008 | 0.0055 | 0.0134 | 0.0054 | 0.0018 | 0.0386 | 0.0047 | 0.0157 | 0.0017 | 0.004 | 0.0066 | 0.0278 | 0.0018 | 0.0213 | 0.0386 | 0.0114 | 0.0052 |
Bud | 0.0015 | 0.0037 | 0.0021 | 0.0024 | 0.0006 | 0.0009 | 0.0021 | 0.0034 | 0.0009 | 0.0002 | 0.0004 | 0.0015 | 0.0033 | 0.0041 | 0.0005 | 0.0005 | 0.0007 | 0.0009 |
Bud Neck | 0.017 | 0.0069 | 0.0058 | 0.0272 | 0.0693 | 0.0826 | 0.0293 | 0.0015 | 0.0098 | 0.0163 | 0.0898 | 0.1015 | 0.006 | 0.0007 | 0.0013 | 0.0019 | 0.1315 | 0.1484 |
Bud Periphery | 0.0039 | 0.0037 | 0.0024 | 0.0027 | 0.0017 | 0.0012 | 0.0027 | 0.0034 | 0.0014 | 0.0009 | 0.0013 | 0.0031 | 0.0076 | 0.0043 | 0.0009 | 0.001 | 0.0023 | 0.0025 |
Bud Site | 0.0067 | 0.0168 | 0.0209 | 0.0091 | 0.0018 | 0.0019 | 0.033 | 0.029 | 0.0049 | 0.0003 | 0.0016 | 0.0004 | 0.0087 | 0.0078 | 0.0161 | 0.0022 | 0.0023 | 0.0014 |
Cell Periphery | 0.0006 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0007 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0006 | 0.0003 |
Cytoplasm | 0.0662 | 0.0744 | 0.0365 | 0.0401 | 0.0444 | 0.0974 | 0.0819 | 0.09 | 0.0241 | 0.008 | 0.0325 | 0.2011 | 0.0501 | 0.1141 | 0.0355 | 0.0047 | 0.0081 | 0.1327 |
Cytoplasmic Foci | 0.0644 | 0.0191 | 0.0504 | 0.007 | 0.0127 | 0.0329 | 0.0823 | 0.0452 | 0.1012 | 0.0021 | 0.0206 | 0.0266 | 0.1112 | 0.0353 | 0.062 | 0.0039 | 0.0216 | 0.0328 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0096 | 0.0057 | 0.0036 | 0.0047 | 0.0049 | 0.0047 | 0.0063 | 0.0026 | 0.0029 | 0.0029 | 0.0075 | 0.0048 | 0.0075 | 0.0045 | 0.0022 | 0.0032 | 0.0057 | 0.0032 |
Endosome | 0.0296 | 0.0064 | 0.0177 | 0.0113 | 0.018 | 0.0098 | 0.0282 | 0.0047 | 0.0283 | 0.0051 | 0.0666 | 0.0096 | 0.0403 | 0.0143 | 0.0149 | 0.0064 | 0.0426 | 0.012 |
Golgi | 0.0028 | 0.0004 | 0.0042 | 0.0044 | 0.0068 | 0.0011 | 0.0145 | 0.0012 | 0.0121 | 0.0002 | 0.0067 | 0.001 | 0.0065 | 0.0075 | 0.0066 | 0.0059 | 0.0095 | 0.0024 |
Lipid Particles | 0.0198 | 0.0035 | 0.0163 | 0.0048 | 0.0399 | 0.0059 | 0.05 | 0.0056 | 0.0239 | 0.0025 | 0.0229 | 0.0046 | 0.0552 | 0.0059 | 0.0287 | 0.0173 | 0.052 | 0.0077 |
Mitochondria | 0.0061 | 0.0013 | 0.0035 | 0.002 | 0.012 | 0.0015 | 0.0095 | 0.0035 | 0.0165 | 0.0018 | 0.0036 | 0.0013 | 0.0131 | 0.0031 | 0.0043 | 0.0049 | 0.0182 | 0.0013 |
None | 0.556 | 0.548 | 0.2642 | 0.008 | 0.0427 | 0.5885 | 0.4386 | 0.499 | 0.1357 | 0.0049 | 0.0049 | 0.5109 | 0.4173 | 0.4429 | 0.211 | 0.0026 | 0.0034 | 0.5522 |
Nuclear Periphery | 0.0686 | 0.0609 | 0.1409 | 0.2659 | 0.2529 | 0.0305 | 0.047 | 0.0762 | 0.1182 | 0.3741 | 0.2981 | 0.0329 | 0.0692 | 0.0744 | 0.1526 | 0.2888 | 0.367 | 0.0249 |
Nucleolus | 0.0157 | 0.0088 | 0.0185 | 0.0106 | 0.0295 | 0.0081 | 0.0071 | 0.0066 | 0.0086 | 0.0108 | 0.0253 | 0.003 | 0.0078 | 0.0066 | 0.0099 | 0.0087 | 0.037 | 0.0031 |
Nucleus | 0.0545 | 0.1573 | 0.1886 | 0.4433 | 0.2816 | 0.0619 | 0.0229 | 0.136 | 0.1246 | 0.5045 | 0.2497 | 0.0378 | 0.0233 | 0.1329 | 0.1418 | 0.4609 | 0.128 | 0.028 |
Peroxisomes | 0.0067 | 0.0015 | 0.003 | 0.0014 | 0.0249 | 0.0012 | 0.025 | 0.0029 | 0.0154 | 0.0002 | 0.0027 | 0.0032 | 0.0326 | 0.0011 | 0.0087 | 0.0083 | 0.0206 | 0.0028 |
Punctate Nuclear | 0.034 | 0.0723 | 0.2072 | 0.1359 | 0.1374 | 0.0617 | 0.0695 | 0.0785 | 0.3486 | 0.0502 | 0.144 | 0.0418 | 0.0939 | 0.1321 | 0.2756 | 0.1331 | 0.1155 | 0.0338 |
Vacuole | 0.0159 | 0.006 | 0.0043 | 0.0024 | 0.0053 | 0.0046 | 0.0068 | 0.0035 | 0.0035 | 0.0041 | 0.009 | 0.0056 | 0.0111 | 0.0039 | 0.0023 | 0.0017 | 0.008 | 0.0029 |
Vacuole Periphery | 0.0081 | 0.002 | 0.0039 | 0.003 | 0.0077 | 0.0018 | 0.0035 | 0.002 | 0.0033 | 0.0092 | 0.0083 | 0.0024 | 0.0065 | 0.0024 | 0.0034 | 0.0052 | 0.0138 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.075 | 53.0911 | 50.4544 | 40.0409 | 19.5882 | 8.8515 | 57.5651 | 72.9379 | 44.9577 | 22.6008 |
Translational Efficiency | 1.0927 | 0.9682 | 0.9339 | 0.7645 | 0.6557 | 0.8499 | 0.909 | 0.693 | 0.8939 | 0.7558 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
300 | 726 | 223 | 888 | 2026 | 1284 | 128 | 96 | 2326 | 2010 | 351 | 984 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 655.95 | 748.17 | 1002.89 | 912.35 | 719.27 | 726.38 | 945.67 | 974.68 | 711.10 | 734.25 | 982.02 | 918.43 |
Standard Deviation | 74.76 | 94.08 | 120.34 | 144.66 | 83.64 | 96.80 | 95.69 | 154.00 | 85.23 | 96.40 | 115.32 | 146.77 |
Intensity Change Log 2 | — | 0.189780 | 0.612506 | 0.476002 | — | 0.014191 | 0.394803 | 0.438395 | — | 0.100612 | 0.502746 | 0.456455 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000127 | 0.000547 | 0.001334 | 0.005485 | 0.000187 | 0.000666 | 0.001003 | 0.001591 | 0.000180 | 0.000623 | 0.001213 | 0.005105 |
Bud Neck | 0.034943 | 0.033885 | 0.010913 | 0.042387 | 0.027308 | 0.050731 | 0.030684 | 0.055243 | 0.028292 | 0.044646 | 0.018123 | 0.043641 |
Bud Site | 0.002641 | 0.011534 | 0.009785 | 0.070533 | 0.006177 | 0.018428 | 0.019241 | 0.085861 | 0.005721 | 0.015938 | 0.013233 | 0.072028 |
Cell Periphery | 0.000311 | 0.000188 | 0.000239 | 0.000481 | 0.000212 | 0.000256 | 0.000328 | 0.000349 | 0.000225 | 0.000231 | 0.000271 | 0.000468 |
Cytoplasm | 0.351682* | 0.304477* | 0.100116* | 0.134020* | 0.435305* | 0.260037* | 0.257449* | 0.074286 | 0.424520* | 0.276088* | 0.157491* | 0.128193* |
Cytoplasmic Foci | 0.235877* | 0.204246* | 0.005249 | 0.034626 | 0.185954* | 0.232143* | 0.030077 | 0.031254 | 0.192393* | 0.222067* | 0.014303 | 0.034297 |
Eisosomes | 0.000231 | 0.000128 | 0.000085 | 0.000106 | 0.000131 | 0.000188 | 0.000152 | 0.000073 | 0.000144 | 0.000167 | 0.000110 | 0.000103 |
Endoplasmic Reticulum | 0.001938 | 0.004192 | 0.022269 | 0.017368 | 0.002037 | 0.003106 | 0.010327 | 0.004032 | 0.002024 | 0.003498 | 0.017914 | 0.016067 |
Endosome | 0.013120 | 0.019155 | 0.004300 | 0.035450 | 0.012712 | 0.032836 | 0.009731 | 0.008749 | 0.012765 | 0.027894 | 0.006280 | 0.032845 |
Golgi | 0.002378 | 0.003443 | 0.000222 | 0.019778 | 0.003410 | 0.009242 | 0.000393 | 0.036403 | 0.003277 | 0.007148 | 0.000284 | 0.021400 |
Lipid Particles | 0.044084 | 0.019472 | 0.002657 | 0.003895 | 0.021879 | 0.043072 | 0.007255 | 0.002130 | 0.024743 | 0.034548 | 0.004334 | 0.003723 |
Mitochondria | 0.013784 | 0.011850 | 0.001080 | 0.006878 | 0.007363 | 0.018811 | 0.000650 | 0.001545 | 0.008191 | 0.016297 | 0.000923 | 0.006358 |
Mitotic Spindle | 0.006782 | 0.039876 | 0.019756 | 0.193585* | 0.010357 | 0.046542 | 0.009785 | 0.256285* | 0.009896 | 0.044134 | 0.016120 | 0.199702* |
None | 0.020430 | 0.011285 | 0.003260 | 0.009488 | 0.017617 | 0.013460 | 0.007455 | 0.017115 | 0.017980 | 0.012675 | 0.004790 | 0.010232 |
Nuclear Periphery | 0.011969 | 0.007901 | 0.005643 | 0.006730 | 0.003747 | 0.005307 | 0.002861 | 0.004681 | 0.004807 | 0.006244 | 0.004629 | 0.006530 |
Nuclear Periphery Foci | 0.032233 | 0.056140 | 0.004043 | 0.019299 | 0.019562 | 0.041570 | 0.007398 | 0.054615 | 0.021197 | 0.046833 | 0.005267 | 0.022745 |
Nucleolus | 0.025885 | 0.025341 | 0.004373 | 0.007151 | 0.016815 | 0.026402 | 0.003731 | 0.030664 | 0.017985 | 0.026018 | 0.004139 | 0.009445 |
Nucleus | 0.169633* | 0.188326* | 0.741699* | 0.258086* | 0.196736* | 0.119225 | 0.479015* | 0.261612* | 0.193240* | 0.144184* | 0.645905* | 0.258430* |
Peroxisomes | 0.012891 | 0.021001 | 0.000652 | 0.015234 | 0.013272 | 0.043106 | 0.019033 | 0.018593 | 0.013222 | 0.035122 | 0.007355 | 0.015562 |
Vacuole | 0.018157 | 0.035602 | 0.061939 | 0.105921 | 0.018452 | 0.032238 | 0.103112 | 0.048735 | 0.018414 | 0.033453 | 0.076954 | 0.100342 |
Vacuole Periphery | 0.000904 | 0.001412 | 0.000388 | 0.013497 | 0.000770 | 0.002632 | 0.000318 | 0.006185 | 0.000787 | 0.002191 | 0.000362 | 0.012784 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.71 | -9.41 | -4.25 | -3.60 | -1.65 | -7.15 | -5.11 | -7.28 | -5.10 | -0.37 | -8.92 | -10.32 | -4.47 | -3.75 | -1.76 |
Bud Neck | 0.11 | 2.13 | -0.30 | -0.69 | -4.55 | -4.78 | 0.78 | -1.74 | 0.54 | -1.90 | -4.12 | 2.45 | -2.29 | 1.76 | -4.18 |
Bud Site | -5.36 | -3.01 | -10.00 | -7.21 | -5.40 | -6.07 | -2.26 | -3.46 | -2.51 | -2.28 | -6.95 | -3.13 | -9.80 | -6.80 | -5.68 |
Cell Periphery | 3.50 | 0.43 | 0.52 | -3.30 | 0.11 | -2.35 | -4.33 | -1.62 | -1.11 | 0.24 | -0.25 | -4.14 | -2.43 | -2.30 | 0.80 |
Cytoplasm | 1.95 | 12.82 | 11.18 | 12.71 | -3.67 | 16.21 | 9.73 | 25.23 | 12.35 | 5.18 | 15.84 | 23.39 | 31.75 | 15.90 | 0.56 |
Cytoplasmic Foci | 2.56 | 17.00 | 16.10 | 21.27 | -6.42 | -4.71 | 24.44 | 24.59 | 25.57 | 1.37 | -2.83 | 37.37 | 37.72 | 37.64 | -1.64 |
Eisosomes | 4.76 | 4.79 | 5.03 | 0.71 | -0.21 | -4.99 | -3.37 | 0.74 | 3.50 | 3.34 | -2.42 | -1.00 | 2.77 | 5.36 | 2.77 |
Endoplasmic Reticulum | -3.05 | -9.07 | -8.36 | -6.80 | 3.21 | -2.27 | -7.77 | -5.04 | -4.40 | 2.68 | -3.72 | -11.56 | -9.13 | -8.00 | 3.32 |
Endosome | -2.72 | 4.10 | -1.03 | 1.79 | -6.15 | -9.49 | 1.73 | 3.33 | 10.46 | 1.11 | -9.86 | 6.26 | -1.29 | 6.24 | -5.01 |
Golgi | -1.30 | 6.23 | -1.73 | -1.22 | -3.36 | -5.07 | 5.30 | -0.61 | 0.14 | -1.03 | -4.60 | 6.03 | -1.45 | 0.77 | -3.44 |
Lipid Particles | 3.87 | 6.40 | 6.36 | 6.64 | -0.80 | -6.19 | 7.64 | 11.42 | 13.07 | 2.09 | -3.19 | 13.12 | 14.69 | 15.10 | 1.85 |
Mitochondria | 0.44 | 2.63 | 2.06 | 3.25 | -6.16 | -6.03 | 7.68 | 5.68 | 10.40 | -4.16 | -4.58 | 7.42 | 4.45 | 9.19 | -6.97 |
Mitotic Spindle | -6.30 | -2.33 | -15.67 | -10.22 | -10.21 | -8.99 | 2.16 | -6.29 | -5.11 | -6.48 | -11.02 | -1.82 | -17.48 | -12.41 | -13.78 |
None | 2.52 | 5.43 | 4.00 | 3.19 | -5.37 | 2.36 | 8.04 | 1.74 | 0.31 | -3.17 | 3.74 | 12.95 | 8.60 | 4.89 | -4.95 |
Nuclear Periphery | 0.79 | 1.35 | 0.45 | -1.06 | -4.22 | -1.41 | -0.21 | -3.01 | -1.57 | -3.05 | -1.15 | 0.15 | -4.40 | -3.15 | -5.60 |
Nuclear Periphery Foci | -1.32 | 5.09 | 1.87 | 4.15 | -7.72 | -4.91 | 2.70 | -0.92 | 0.97 | -1.83 | -5.93 | 7.15 | -0.98 | 4.54 | -6.84 |
Nucleolus | 0.84 | 5.78 | 3.65 | 4.54 | -5.76 | -2.66 | 9.06 | 1.81 | 3.30 | -2.77 | -2.51 | 11.62 | 4.11 | 6.28 | -6.58 |
Nucleus | -1.83 | -25.01 | -10.38 | -9.66 | 17.55 | 10.28 | -10.44 | -4.09 | -6.23 | 3.37 | 6.93 | -25.54 | -11.96 | -15.89 | 15.25 |
Peroxisomes | -1.82 | 5.97 | 3.30 | 6.61 | -4.96 | -9.99 | 0.33 | 3.75 | 11.07 | 1.24 | -9.90 | 4.23 | 5.31 | 13.37 | -0.68 |
Vacuole | -4.90 | -7.79 | -17.52 | -15.19 | -5.18 | -5.77 | -8.99 | -4.53 | -4.02 | 1.50 | -8.01 | -11.68 | -18.72 | -16.52 | -2.76 |
Vacuole Periphery | -2.59 | 2.95 | -2.53 | -2.14 | -2.90 | -4.88 | 6.10 | -0.71 | 0.66 | -1.01 | -5.57 | 6.30 | -2.68 | -1.47 | -3.00 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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