Standard name | |
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Human Ortholog | |
Description | Protein involved in phospholipid flippase function; null allele suppresses growth and membrane trafficking defects associated with all flippase null alleles; proposed function as a phospholipid scramblase that reduces membrane asymmetry; PQ loop family member; localizes to the endosome and trans-Golgi network; non-essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 6 | 7 | 7 | 3 | 3 | 6 | 8 | 9 | 13 | 1 | 8 | 8 | 3 | 2 | 20 | 20 | 3 | 2 | 1 | 9 | 0 | 1 | 4 |
Bud | 0 | 1 | 4 | 2 | 0 | 6 | 6 | 8 | 17 | 26 | 24 | 26 | 2 | 0 | 1 | 2 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 3 | 6 | 3 | 9 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 3 | 10 | 12 | 28 | 44 | 52 | 60 | 51 | 56 | 40 | 57 | 1 | 13 | 5 | 30 | 28 | 15 | 0 | 0 | 0 | 0 | 1 | 2 |
Endoplasmic Reticulum | 2 | 4 | 3 | 1 | 1 | 2 | 6 | 4 | 8 | 4 | 3 | 6 | 5 | 3 | 4 | 16 | 8 | 3 | 0 | 0 | 1 | 0 | 1 | 1 |
Endosome | 3 | 1 | 37 | 31 | 60 | 45 | 53 | 86 | 46 | 57 | 57 | 78 | 5 | 7 | 20 | 19 | 19 | 12 | 11 | 17 | 27 | 19 | 24 | 20 |
Golgi | 29 | 42 | 100 | 58 | 54 | 19 | 27 | 29 | 47 | 23 | 12 | 15 | 52 | 78 | 117 | 133 | 82 | 39 | 74 | 69 | 158 | 70 | 107 | 149 |
Mitochondria | 67 | 30 | 106 | 92 | 55 | 70 | 111 | 56 | 98 | 132 | 119 | 111 | 28 | 20 | 24 | 19 | 13 | 7 | 2 | 3 | 7 | 6 | 8 | 4 |
Nucleus | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 6 | 3 | 8 | 9 | 6 | 16 | 17 | 11 | 9 | 12 | 8 | 7 | 4 | 2 | 1 | 7 | 14 | 3 | 0 | 0 | 0 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 2 | 5 | 0 | 1 | 4 | 10 | 4 | 6 | 4 | 8 | 1 | 1 | 4 | 1 | 7 | 3 | 0 | 0 | 1 | 1 | 0 | 0 |
Vac/Vac Membrane | 14 | 20 | 21 | 19 | 70 | 50 | 81 | 68 | 57 | 52 | 51 | 51 | 12 | 13 | 16 | 94 | 70 | 61 | 4 | 10 | 2 | 6 | 7 | 7 |
Unique Cell Count | 81 | 72 | 199 | 160 | 210 | 207 | 287 | 266 | 265 | 300 | 251 | 302 | 77 | 109 | 150 | 230 | 185 | 110 | 99 | 108 | 222 | 111 | 155 | 196 |
Labelled Cell Count | 123 | 111 | 302 | 238 | 277 | 257 | 369 | 346 | 351 | 391 | 327 | 382 | 118 | 142 | 196 | 342 | 270 | 148 | 99 | 108 | 222 | 111 | 155 | 196 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.7 | 13.6 | 11.6 | 10.2 | 11.2 | 9.7 | 9.5 | 9.9 | 9.1 | 9.1 | 8.5 | 8.6 | 11.6 | 12.1 | 11.2 | 15.0 | 13.8 | 14.3 | 12.8 | 12.9 | 13.8 |
Std Deviation (1e-4) | 1.4 | 2.6 | 1.7 | 1.7 | 1.9 | 1.9 | 1.8 | 2.1 | 1.6 | 1.7 | 1.5 | 1.6 | 2.2 | 2.2 | 1.8 | 3.1 | 3.0 | 3.2 | 2.1 | 2.0 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.05 | -0.26 | -0.29 | -0.24 | -0.35 | -0.36 | -0.45 | -0.43 | 0.0 | 0.06 | -0.06 | 0.37 | 0.25 | 0.3 | 0.14 | 0.15 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.4 | 0 | 0 | -1.0 | -0.3 | -0.1 | 0.5 | 0 | -0.6 | 0 | 0 | 0 | 2.2 | 2.8 | 0 |
Bud | 0 | 0 | 0 | 0 | 0.7 | 2.3 | 3.1 | 3.3 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 2.9 | 4.8 | 4.3 | 5.2 | 4.5 | 4.4 | 3.7 | 4.5 | 0 | 2.2 | -0.8 | 2.8 | 3.3 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 1.7 | 0 |
Endosome | 0.2 | 2.4 | 0.8 | 0 | 3.3 | -0.3 | 0.1 | 1.1 | 1.9 | -2.5 | -2.9 | -1.3 | -3.2 | -2.3 | -1.8 |
Golgi | -2.7 | -5.1 | -9.1 | -10.1 | -9.4 | -7.5 | -10.8 | -11.1 | -11.8 | 2.6 | 3.6 | 5.3 | 1.6 | -1.2 | -2.5 |
Mitochondria | 0.8 | -5.6 | -4.0 | -3.2 | -7.2 | -3.5 | -2.0 | -1.2 | -3.6 | -2.5 | -6.0 | -7.1 | -10.2 | -9.8 | -8.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.7 | -0.6 | 1.6 | 0.9 | 0.1 | -0.4 | 0 | -0.5 | -1.1 | 0 | 0 | 0 | -0.5 | 1.5 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.4 | 5.5 | 3.6 | 4.7 | 4.1 | 3.1 | 2.1 | 2.8 | 2.0 | 1.2 | 0.4 | 0 | 7.1 | 6.3 | 8.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.3751 | 7.8947 | 7.2659 | 6.432 | 6.4371 | 7.2183 | 7.4565 | 10.8062 | 10.1757 | 9.4012 | 9.8403 | 10.4083 | 7.4124 | 9.8386 | 9.3457 | 8.6131 | 7.2862 | 8.9852 |
Actin | 0.0244 | 0.013 | 0.0165 | 0.0065 | 0.0052 | 0.0208 | 0.0365 | 0.0078 | 0.006 | 0.0023 | 0.002 | 0.0096 | 0.0305 | 0.0032 | 0.0064 | 0.0016 | 0.0235 | 0.0026 |
Bud | 0.0003 | 0.0001 | 0.0003 | 0.0008 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0006 | 0.0002 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.007 | 0.0005 | 0.0002 |
Bud Periphery | 0.0003 | 0.0001 | 0.0003 | 0.0009 | 0.0002 | 0.0001 | 0.0015 | 0.0002 | 0.0007 | 0.0003 | 0.0004 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0015 | 0.0002 | 0.0007 | 0.0013 | 0.0001 | 0.0001 | 0.0034 | 0.0005 | 0.0011 | 0.0029 | 0.0009 | 0.0001 | 0.0055 | 0.0002 | 0.0005 | 0.001 | 0.0003 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0024 | 0.0024 | 0.0019 | 0.0008 | 0.0008 | 0.0008 | 0.0135 | 0.0017 | 0.0012 | 0.0014 | 0.001 | 0.0005 | 0.0016 | 0.0013 | 0.0016 | 0.0009 | 0.0025 | 0.0016 |
Cytoplasmic Foci | 0.0719 | 0.0785 | 0.0576 | 0.0524 | 0.0377 | 0.0336 | 0.0595 | 0.0651 | 0.0498 | 0.0416 | 0.0205 | 0.032 | 0.0501 | 0.0528 | 0.0361 | 0.0572 | 0.0329 | 0.0442 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0011 | 0.0004 | 0.0005 | 0.0006 | 0.0005 | 0.0007 | 0.005 | 0.0003 | 0.0008 | 0.0003 | 0.0013 | 0.0004 | 0.0015 | 0.0003 | 0.0006 | 0.0004 | 0.0013 | 0.0005 |
Endosome | 0.2619 | 0.2474 | 0.2059 | 0.2232 | 0.3203 | 0.1884 | 0.1729 | 0.2285 | 0.2013 | 0.2207 | 0.3102 | 0.1518 | 0.1737 | 0.1986 | 0.1651 | 0.1596 | 0.1759 | 0.1225 |
Golgi | 0.5337 | 0.6334 | 0.6452 | 0.582 | 0.589 | 0.7134 | 0.5986 | 0.662 | 0.7032 | 0.6487 | 0.598 | 0.7779 | 0.6413 | 0.7093 | 0.7416 | 0.7326 | 0.6009 | 0.7937 |
Lipid Particles | 0.0428 | 0.0021 | 0.0213 | 0.0132 | 0.0079 | 0.0205 | 0.0268 | 0.0043 | 0.0065 | 0.0209 | 0.0148 | 0.0082 | 0.0262 | 0.0152 | 0.0063 | 0.0223 | 0.1089 | 0.0119 |
Mitochondria | 0.0286 | 0.0071 | 0.0156 | 0.0217 | 0.0272 | 0.0095 | 0.017 | 0.0214 | 0.0069 | 0.0437 | 0.0263 | 0.0133 | 0.0251 | 0.0101 | 0.0177 | 0.004 | 0.014 | 0.0134 |
None | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0038 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0013 | 0 | 0.0012 | 0.0003 | 0.0055 | 0.0001 |
Nuclear Periphery | 0.0012 | 0.0001 | 0.0004 | 0.0005 | 0.0003 | 0.0004 | 0.0142 | 0.0001 | 0.0013 | 0.0002 | 0.0019 | 0.0004 | 0.0081 | 0.0002 | 0.0012 | 0.0002 | 0.0105 | 0.0006 |
Nucleolus | 0.0004 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.001 | 0 | 0.0001 | 0.0018 | 0.0001 | 0 | 0.0003 | 0 | 0.0007 | 0.0007 | 0.0006 | 0.0001 |
Nucleus | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0179 | 0 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0012 | 0 | 0.0073 | 0.0003 | 0.003 | 0.0001 |
Peroxisomes | 0.0154 | 0.0089 | 0.0194 | 0.0159 | 0.0013 | 0.0023 | 0.0116 | 0.0023 | 0.0082 | 0.0046 | 0.0016 | 0.0017 | 0.0125 | 0.0028 | 0.0023 | 0.0039 | 0.004 | 0.0021 |
Punctate Nuclear | 0.0012 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0061 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0037 | 0.0001 | 0.0027 | 0.003 | 0.0095 | 0.0003 |
Vacuole | 0.0057 | 0.0025 | 0.0054 | 0.0573 | 0.0024 | 0.0037 | 0.005 | 0.0021 | 0.0051 | 0.0044 | 0.0074 | 0.0009 | 0.0101 | 0.002 | 0.0041 | 0.0018 | 0.0014 | 0.0018 |
Vacuole Periphery | 0.0056 | 0.0035 | 0.0078 | 0.0214 | 0.0065 | 0.0052 | 0.0033 | 0.0031 | 0.0061 | 0.0044 | 0.0124 | 0.0026 | 0.0048 | 0.0034 | 0.0041 | 0.0028 | 0.0042 | 0.0041 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 55.7138 | 34.013 | 35.4549 | 59.0473 | 43.4587 | 58.8519 | 51.9951 | 54.4359 | 45.5796 | 59.2404 |
Translational Efficiency | 0.5228 | 0.938 | 0.722 | 0.3847 | 0.5705 | 0.5018 | 0.5401 | 0.4028 | 0.5369 | 0.4806 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
112 | 171 | 1741 | 1669 | 1413 | 1679 | 219 | 1170 | 1525 | 1850 | 1960 | 2839 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1122.05 | 1244.15 | 1381.24 | 1385.95 | 1001.14 | 1097.48 | 1497.82 | 1314.60 | 1010.02 | 1111.04 | 1394.27 | 1356.55 |
Standard Deviation | 146.47 | 167.42 | 191.64 | 210.07 | 143.84 | 158.50 | 168.16 | 228.83 | 147.44 | 164.91 | 192.69 | 220.81 |
Intensity Change Log 2 | — | 0.149023 | 0.299827 | 0.304738 | — | 0.132551 | 0.581221 | 0.392980 | — | 0.141280 | 0.439364 | 0.347020 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000221 | 0.003087 | 0.009751 | 0.005578 | 0.002094 | 0.002596 | 0.005387 | 0.012265 | 0.001956 | 0.002641 | 0.009264 | 0.008334 |
Bud Neck | 0.000076 | 0.000277 | 0.000425 | 0.000385 | 0.000139 | 0.000249 | 0.000609 | 0.000554 | 0.000135 | 0.000251 | 0.000445 | 0.000454 |
Bud Site | 0.001524 | 0.005600 | 0.000966 | 0.004053 | 0.001254 | 0.001624 | 0.002321 | 0.010282 | 0.001274 | 0.001991 | 0.001118 | 0.006620 |
Cell Periphery | 0.000065 | 0.000083 | 0.000102 | 0.000122 | 0.000062 | 0.000090 | 0.000084 | 0.000202 | 0.000062 | 0.000090 | 0.000100 | 0.000155 |
Cytoplasm | 0.000591 | 0.000833 | 0.000344 | 0.001441 | 0.000723 | 0.000774 | 0.000347 | 0.002972 | 0.000714 | 0.000779 | 0.000344 | 0.002072 |
Cytoplasmic Foci | 0.167697 | 0.146268 | 0.103811 | 0.073097 | 0.197095* | 0.162848 | 0.120001 | 0.101520 | 0.194936* | 0.161316 | 0.105620 | 0.084810 |
Eisosomes | 0.000012 | 0.000026 | 0.000080 | 0.000050 | 0.000033 | 0.000042 | 0.000034 | 0.000081 | 0.000032 | 0.000040 | 0.000075 | 0.000063 |
Endoplasmic Reticulum | 0.000367 | 0.000434 | 0.001298 | 0.000593 | 0.000582 | 0.000439 | 0.000822 | 0.001483 | 0.000566 | 0.000438 | 0.001245 | 0.000960 |
Endosome | 0.169560 | 0.168459 | 0.069711 | 0.103230 | 0.126858 | 0.195872 | 0.111979 | 0.138715 | 0.129994 | 0.193338 | 0.074434 | 0.117854 |
Golgi | 0.616347* | 0.605446* | 0.733322* | 0.736645* | 0.621801* | 0.561793* | 0.708908* | 0.658592* | 0.621401* | 0.565828* | 0.730594* | 0.704478* |
Lipid Particles | 0.000346 | 0.001591 | 0.038454 | 0.006203 | 0.007958 | 0.007323 | 0.005096 | 0.007220 | 0.007399 | 0.006793 | 0.034726 | 0.006622 |
Mitochondria | 0.003564 | 0.012835 | 0.009695 | 0.011868 | 0.017961 | 0.023209 | 0.002602 | 0.009080 | 0.016903 | 0.022250 | 0.008903 | 0.010719 |
Mitotic Spindle | 0.000540 | 0.002383 | 0.001912 | 0.005141 | 0.001910 | 0.001474 | 0.005823 | 0.006984 | 0.001809 | 0.001558 | 0.002349 | 0.005900 |
None | 0.000012 | 0.000023 | 0.000054 | 0.000083 | 0.000033 | 0.000053 | 0.000023 | 0.000067 | 0.000032 | 0.000050 | 0.000051 | 0.000076 |
Nuclear Periphery | 0.000033 | 0.000056 | 0.000631 | 0.000261 | 0.000115 | 0.000129 | 0.000262 | 0.000121 | 0.000109 | 0.000122 | 0.000590 | 0.000203 |
Nuclear Periphery Foci | 0.000168 | 0.000222 | 0.000940 | 0.000994 | 0.000982 | 0.000692 | 0.000783 | 0.000541 | 0.000922 | 0.000649 | 0.000923 | 0.000807 |
Nucleolus | 0.000002 | 0.000006 | 0.000008 | 0.000015 | 0.000027 | 0.000337 | 0.000005 | 0.000017 | 0.000025 | 0.000306 | 0.000007 | 0.000016 |
Nucleus | 0.000005 | 0.000012 | 0.000014 | 0.000027 | 0.000060 | 0.000145 | 0.000010 | 0.000079 | 0.000056 | 0.000133 | 0.000014 | 0.000048 |
Peroxisomes | 0.001580 | 0.002357 | 0.000729 | 0.000964 | 0.001095 | 0.002913 | 0.001920 | 0.002709 | 0.001131 | 0.002862 | 0.000862 | 0.001683 |
Vacuole | 0.019913 | 0.028058 | 0.013151 | 0.030837 | 0.008044 | 0.019242 | 0.021012 | 0.030917 | 0.008916 | 0.020056 | 0.014030 | 0.030870 |
Vacuole Periphery | 0.017376 | 0.021943 | 0.014602 | 0.018415 | 0.011175 | 0.018157 | 0.011970 | 0.015598 | 0.011630 | 0.018507 | 0.014308 | 0.017254 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.32 | -7.60 | -5.49 | -1.06 | 2.65 | -0.68 | -1.33 | -5.17 | -4.91 | -2.22 | -0.98 | -5.92 | -5.91 | -5.18 | 0.65 |
Bud Neck | -2.63 | -6.89 | -6.77 | -1.36 | 0.49 | -2.50 | -3.43 | -7.37 | -4.80 | 0.17 | -2.88 | -6.45 | -8.67 | -4.35 | -0.46 |
Bud Site | -1.33 | 1.27 | -3.69 | 0.43 | -4.88 | -1.08 | -1.57 | -6.48 | -6.19 | -5.31 | -1.70 | 0.52 | -7.70 | -6.18 | -7.89 |
Cell Periphery | -0.73 | -1.58 | -3.02 | -1.88 | -0.95 | -2.10 | -1.50 | -5.55 | -4.07 | -4.17 | -2.20 | -2.38 | -7.50 | -4.08 | -2.83 |
Cytoplasm | -0.94 | 1.70 | -2.44 | -1.61 | -3.23 | -0.21 | 1.90 | -3.89 | -3.88 | -4.53 | -0.30 | 2.00 | -4.12 | -4.15 | -5.54 |
Cytoplasmic Foci | 1.31 | 4.76 | 7.11 | 7.24 | 8.46 | 6.57 | 8.50 | 18.17 | 12.25 | 2.00 | 6.76 | 19.51 | 26.06 | 19.77 | 6.16 |
Eisosomes | -2.12 | -13.46 | -7.94 | -3.10 | 4.67 | -2.23 | -0.12 | -6.56 | -5.28 | -5.66 | -2.44 | -9.21 | -7.09 | -4.87 | 2.13 |
Endoplasmic Reticulum | -0.29 | -3.92 | -1.58 | -0.80 | 3.36 | 1.44 | -1.34 | -1.99 | -2.33 | -1.40 | 1.36 | -3.49 | -1.95 | -2.68 | 1.11 |
Endosome | 0.06 | 7.05 | 4.63 | 5.59 | -9.18 | -11.96 | 1.64 | -2.14 | 9.24 | -2.84 | -11.55 | 13.62 | 2.86 | 15.30 | -13.19 |
Golgi | 0.39 | -5.37 | -5.45 | -6.55 | -0.30 | 7.00 | -5.11 | -3.38 | -9.15 | 2.96 | 6.78 | -14.04 | -10.82 | -17.71 | 3.82 |
Lipid Particles | -1.66 | -15.67 | -7.23 | -4.20 | 12.59 | 0.39 | 1.78 | 0.40 | 0.05 | -1.37 | 0.40 | -10.97 | 0.55 | 0.14 | 12.29 |
Mitochondria | -1.45 | -2.94 | -3.62 | 0.15 | -1.07 | -1.89 | 7.30 | 3.78 | 5.95 | -4.21 | -2.04 | 3.74 | 2.92 | 5.30 | -1.16 |
Mitotic Spindle | -1.42 | -2.98 | -4.71 | -1.77 | -3.08 | 0.89 | -1.00 | -3.47 | -3.77 | -0.34 | 0.54 | -0.81 | -4.68 | -4.98 | -3.61 |
None | -1.61 | -10.46 | -1.52 | -1.28 | -0.61 | -1.61 | 2.29 | -2.07 | -0.86 | -2.66 | -1.66 | -4.37 | -1.62 | -0.92 | -0.95 |
Nuclear Periphery | -1.24 | -6.37 | -5.09 | -4.50 | 3.58 | -0.29 | -2.06 | -0.17 | 0.20 | 2.34 | -0.29 | -5.23 | -2.01 | -2.29 | 4.42 |
Nuclear Periphery Foci | -0.71 | -5.87 | -2.38 | -2.24 | -0.17 | 0.64 | 0.45 | 0.99 | 0.80 | 1.23 | 0.66 | -0.00 | 0.23 | -0.69 | 0.44 |
Nucleolus | -1.21 | -4.34 | -3.80 | -1.99 | -2.13 | -1.30 | 1.61 | 0.59 | 1.34 | -3.34 | -1.30 | 1.42 | 0.68 | 1.34 | -3.45 |
Nucleus | -1.59 | -6.29 | -2.75 | -1.69 | -1.58 | -1.07 | 1.42 | -0.96 | -0.55 | -1.20 | -1.06 | 1.28 | -0.76 | 0.04 | -1.27 |
Peroxisomes | -0.55 | 2.00 | 1.36 | 1.01 | -1.21 | -3.76 | -1.16 | -3.86 | 0.29 | -1.05 | -3.78 | 1.32 | -2.24 | 2.50 | -3.75 |
Vacuole | -1.00 | 1.09 | -1.76 | -0.53 | -8.60 | -7.24 | -3.74 | -10.38 | -4.71 | -2.58 | -7.13 | -4.66 | -13.85 | -5.61 | -10.37 |
Vacuole Periphery | -0.71 | 0.62 | -0.23 | 0.73 | -2.98 | -4.61 | -0.38 | -2.73 | 1.41 | -1.57 | -4.62 | -2.45 | -4.60 | 0.86 | -2.77 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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