Standard name | |
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Human Ortholog | |
Description | Protein involved in phospholipid flippase function; null allele suppresses growth and membrane trafficking defects associated with all flippase null alleles; proposed function as a phospholipid scramblase that reduces membrane asymmetry; PQ loop family member; localizes to the endosome and trans-Golgi network; non-essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 6 | 7 | 7 | 3 | 3 | 6 | 8 | 9 | 13 | 1 | 8 | 8 | 3 | 2 | 20 | 20 | 3 | 2 | 1 | 9 | 0 | 1 | 4 |
Bud | 0 | 1 | 4 | 2 | 0 | 6 | 6 | 8 | 17 | 26 | 24 | 26 | 2 | 0 | 1 | 2 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 3 | 6 | 3 | 9 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 3 | 10 | 12 | 28 | 44 | 52 | 60 | 51 | 56 | 40 | 57 | 1 | 13 | 5 | 30 | 28 | 15 | 0 | 0 | 0 | 0 | 1 | 2 |
Endoplasmic Reticulum | 2 | 4 | 3 | 1 | 1 | 2 | 6 | 4 | 8 | 4 | 3 | 6 | 5 | 3 | 4 | 16 | 8 | 3 | 0 | 0 | 1 | 0 | 1 | 1 |
Endosome | 3 | 1 | 37 | 31 | 60 | 45 | 53 | 86 | 46 | 57 | 57 | 78 | 5 | 7 | 20 | 19 | 19 | 12 | 11 | 17 | 27 | 19 | 24 | 20 |
Golgi | 29 | 42 | 100 | 58 | 54 | 19 | 27 | 29 | 47 | 23 | 12 | 15 | 52 | 78 | 117 | 133 | 82 | 39 | 74 | 69 | 158 | 70 | 107 | 149 |
Mitochondria | 67 | 30 | 106 | 92 | 55 | 70 | 111 | 56 | 98 | 132 | 119 | 111 | 28 | 20 | 24 | 19 | 13 | 7 | 2 | 3 | 7 | 6 | 8 | 4 |
Nucleus | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 6 | 3 | 8 | 9 | 6 | 16 | 17 | 11 | 9 | 12 | 8 | 7 | 4 | 2 | 1 | 7 | 14 | 3 | 0 | 0 | 0 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 2 | 5 | 0 | 1 | 4 | 10 | 4 | 6 | 4 | 8 | 1 | 1 | 4 | 1 | 7 | 3 | 0 | 0 | 1 | 1 | 0 | 0 |
Vac/Vac Membrane | 14 | 20 | 21 | 19 | 70 | 50 | 81 | 68 | 57 | 52 | 51 | 51 | 12 | 13 | 16 | 94 | 70 | 61 | 4 | 10 | 2 | 6 | 7 | 7 |
Unique Cell Count | 81 | 72 | 199 | 160 | 210 | 207 | 287 | 266 | 265 | 300 | 251 | 302 | 77 | 109 | 150 | 230 | 185 | 110 | 99 | 108 | 222 | 111 | 155 | 196 |
Labelled Cell Count | 123 | 111 | 302 | 238 | 277 | 257 | 369 | 346 | 351 | 391 | 327 | 382 | 118 | 142 | 196 | 342 | 270 | 148 | 99 | 108 | 222 | 111 | 155 | 196 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.7 | 13.6 | 11.6 | 10.2 | 11.2 | 9.7 | 9.5 | 9.9 | 9.1 | 9.1 | 8.5 | 8.6 | 11.6 | 12.1 | 11.2 | 15.0 | 13.8 | 14.3 | 12.8 | 12.9 | 13.8 |
Std Deviation (1e-4) | 1.4 | 2.6 | 1.7 | 1.7 | 1.9 | 1.9 | 1.8 | 2.1 | 1.6 | 1.7 | 1.5 | 1.6 | 2.2 | 2.2 | 1.8 | 3.1 | 3.0 | 3.2 | 2.1 | 2.0 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.05 | -0.26 | -0.29 | -0.24 | -0.35 | -0.36 | -0.45 | -0.43 | 0.0 | 0.06 | -0.06 | 0.37 | 0.25 | 0.3 | 0.14 | 0.15 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.4 | 0 | 0 | -1.0 | -0.3 | -0.1 | 0.5 | 0 | -0.6 | 0 | 0 | 0 | 2.2 | 2.8 | 0 |
Bud | 0 | 0 | 0 | 0 | 0.7 | 2.3 | 3.1 | 3.3 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 2.9 | 4.8 | 4.3 | 5.2 | 4.5 | 4.4 | 3.7 | 4.5 | 0 | 2.2 | -0.8 | 2.8 | 3.3 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 1.7 | 0 |
Endosome | 0.2 | 2.4 | 0.8 | 0 | 3.3 | -0.3 | 0.1 | 1.1 | 1.9 | -2.5 | -2.9 | -1.3 | -3.2 | -2.3 | -1.8 |
Golgi | -2.7 | -5.1 | -9.1 | -10.1 | -9.4 | -7.5 | -10.8 | -11.1 | -11.8 | 2.6 | 3.6 | 5.3 | 1.6 | -1.2 | -2.5 |
Mitochondria | 0.8 | -5.6 | -4.0 | -3.2 | -7.2 | -3.5 | -2.0 | -1.2 | -3.6 | -2.5 | -6.0 | -7.1 | -10.2 | -9.8 | -8.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.7 | -0.6 | 1.6 | 0.9 | 0.1 | -0.4 | 0 | -0.5 | -1.1 | 0 | 0 | 0 | -0.5 | 1.5 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.4 | 5.5 | 3.6 | 4.7 | 4.1 | 3.1 | 2.1 | 2.8 | 2.0 | 1.2 | 0.4 | 0 | 7.1 | 6.3 | 8.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.3751 | 7.8947 | 7.2659 | 6.432 | 6.4371 | 7.2183 | 7.4565 | 10.8062 | 10.1757 | 9.4012 | 9.8403 | 10.4083 | 7.4124 | 9.8386 | 9.3457 | 8.6131 | 7.2862 | 8.9852 |
Actin | 0.0244 | 0.013 | 0.0165 | 0.0065 | 0.0052 | 0.0208 | 0.0365 | 0.0078 | 0.006 | 0.0023 | 0.002 | 0.0096 | 0.0305 | 0.0032 | 0.0064 | 0.0016 | 0.0235 | 0.0026 |
Bud | 0.0003 | 0.0001 | 0.0003 | 0.0008 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0006 | 0.0002 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.007 | 0.0005 | 0.0002 |
Bud Periphery | 0.0003 | 0.0001 | 0.0003 | 0.0009 | 0.0002 | 0.0001 | 0.0015 | 0.0002 | 0.0007 | 0.0003 | 0.0004 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0015 | 0.0002 | 0.0007 | 0.0013 | 0.0001 | 0.0001 | 0.0034 | 0.0005 | 0.0011 | 0.0029 | 0.0009 | 0.0001 | 0.0055 | 0.0002 | 0.0005 | 0.001 | 0.0003 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0024 | 0.0024 | 0.0019 | 0.0008 | 0.0008 | 0.0008 | 0.0135 | 0.0017 | 0.0012 | 0.0014 | 0.001 | 0.0005 | 0.0016 | 0.0013 | 0.0016 | 0.0009 | 0.0025 | 0.0016 |
Cytoplasmic Foci | 0.0719 | 0.0785 | 0.0576 | 0.0524 | 0.0377 | 0.0336 | 0.0595 | 0.0651 | 0.0498 | 0.0416 | 0.0205 | 0.032 | 0.0501 | 0.0528 | 0.0361 | 0.0572 | 0.0329 | 0.0442 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0011 | 0.0004 | 0.0005 | 0.0006 | 0.0005 | 0.0007 | 0.005 | 0.0003 | 0.0008 | 0.0003 | 0.0013 | 0.0004 | 0.0015 | 0.0003 | 0.0006 | 0.0004 | 0.0013 | 0.0005 |
Endosome | 0.2619 | 0.2474 | 0.2059 | 0.2232 | 0.3203 | 0.1884 | 0.1729 | 0.2285 | 0.2013 | 0.2207 | 0.3102 | 0.1518 | 0.1737 | 0.1986 | 0.1651 | 0.1596 | 0.1759 | 0.1225 |
Golgi | 0.5337 | 0.6334 | 0.6452 | 0.582 | 0.589 | 0.7134 | 0.5986 | 0.662 | 0.7032 | 0.6487 | 0.598 | 0.7779 | 0.6413 | 0.7093 | 0.7416 | 0.7326 | 0.6009 | 0.7937 |
Lipid Particles | 0.0428 | 0.0021 | 0.0213 | 0.0132 | 0.0079 | 0.0205 | 0.0268 | 0.0043 | 0.0065 | 0.0209 | 0.0148 | 0.0082 | 0.0262 | 0.0152 | 0.0063 | 0.0223 | 0.1089 | 0.0119 |
Mitochondria | 0.0286 | 0.0071 | 0.0156 | 0.0217 | 0.0272 | 0.0095 | 0.017 | 0.0214 | 0.0069 | 0.0437 | 0.0263 | 0.0133 | 0.0251 | 0.0101 | 0.0177 | 0.004 | 0.014 | 0.0134 |
None | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0038 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0013 | 0 | 0.0012 | 0.0003 | 0.0055 | 0.0001 |
Nuclear Periphery | 0.0012 | 0.0001 | 0.0004 | 0.0005 | 0.0003 | 0.0004 | 0.0142 | 0.0001 | 0.0013 | 0.0002 | 0.0019 | 0.0004 | 0.0081 | 0.0002 | 0.0012 | 0.0002 | 0.0105 | 0.0006 |
Nucleolus | 0.0004 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.001 | 0 | 0.0001 | 0.0018 | 0.0001 | 0 | 0.0003 | 0 | 0.0007 | 0.0007 | 0.0006 | 0.0001 |
Nucleus | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0179 | 0 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0012 | 0 | 0.0073 | 0.0003 | 0.003 | 0.0001 |
Peroxisomes | 0.0154 | 0.0089 | 0.0194 | 0.0159 | 0.0013 | 0.0023 | 0.0116 | 0.0023 | 0.0082 | 0.0046 | 0.0016 | 0.0017 | 0.0125 | 0.0028 | 0.0023 | 0.0039 | 0.004 | 0.0021 |
Punctate Nuclear | 0.0012 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0061 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0037 | 0.0001 | 0.0027 | 0.003 | 0.0095 | 0.0003 |
Vacuole | 0.0057 | 0.0025 | 0.0054 | 0.0573 | 0.0024 | 0.0037 | 0.005 | 0.0021 | 0.0051 | 0.0044 | 0.0074 | 0.0009 | 0.0101 | 0.002 | 0.0041 | 0.0018 | 0.0014 | 0.0018 |
Vacuole Periphery | 0.0056 | 0.0035 | 0.0078 | 0.0214 | 0.0065 | 0.0052 | 0.0033 | 0.0031 | 0.0061 | 0.0044 | 0.0124 | 0.0026 | 0.0048 | 0.0034 | 0.0041 | 0.0028 | 0.0042 | 0.0041 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 55.7138 | 34.013 | 35.4549 | 59.0473 | 43.4587 | 58.8519 | 51.9951 | 54.4359 | 45.5796 | 59.2404 |
Translational Efficiency | 0.5228 | 0.938 | 0.722 | 0.3847 | 0.5705 | 0.5018 | 0.5401 | 0.4028 | 0.5369 | 0.4806 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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