Standard name | |
---|---|
Human Ortholog | |
Description | Subunit a of the vacuolar-ATPase V0 domain; one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 13 | 6 | 0 | 1 | 1 | 0 | 1 | – | 1 | 5 | 0 | 2 | 3 | 1 | 4 | 10 | 3 | 10 | 8 | 6 | 1 | 4 | 5 |
Bud | 9 | 3 | 10 | 12 | 9 | 9 | 24 | 23 | – | 20 | 19 | 25 | 0 | 0 | 0 | 4 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 2 | 0 | 0 | 2 | 3 | 1 | – | 4 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 33 | 12 | 23 | 22 | 33 | 32 | 32 | 26 | – | 22 | 14 | 17 | 2 | 3 | 13 | 62 | 21 | 17 | 3 | 1 | 3 | 0 | 1 | 0 |
Endoplasmic Reticulum | 10 | 9 | 3 | 0 | 1 | 3 | 3 | 1 | – | 5 | 1 | 5 | 0 | 1 | 4 | 16 | 2 | 2 | 0 | 0 | 2 | 0 | 2 | 0 |
Endosome | 128 | 5 | 109 | 72 | 76 | 10 | 7 | 26 | – | 4 | 4 | 10 | 2 | 3 | 33 | 87 | 13 | 17 | 152 | 47 | 115 | 14 | 42 | 44 |
Golgi | 23 | 8 | 13 | 12 | 10 | 1 | 0 | 0 | – | 1 | 3 | 0 | 0 | 4 | 21 | 4 | 3 | 4 | 138 | 34 | 91 | 13 | 40 | 48 |
Mitochondria | 178 | 116 | 83 | 113 | 95 | 240 | 270 | 222 | – | 268 | 227 | 306 | 13 | 15 | 26 | 30 | 5 | 8 | 69 | 36 | 25 | 10 | 25 | 25 |
Nucleus | 2 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | – | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | – | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 0 | 1 | 3 | 6 | 6 | 7 | – | 6 | 4 | 2 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 29 | 0 | 45 | 16 | 10 | 6 | 26 | 9 | – | 7 | 6 | 2 | 0 | 2 | 4 | 11 | 0 | 0 | 50 | 15 | 62 | 10 | 12 | 12 |
SpindlePole | 36 | 6 | 51 | 14 | 14 | 11 | 24 | 28 | – | 11 | 11 | 10 | 2 | 0 | 7 | 56 | 8 | 10 | 14 | 3 | 3 | 0 | 1 | 1 |
Vac/Vac Membrane | 31 | 5 | 7 | 15 | 16 | 11 | 12 | 6 | – | 7 | 12 | 15 | 0 | 1 | 6 | 35 | 7 | 4 | 14 | 4 | 3 | 0 | 5 | 7 |
Unique Cell Count | 361 | 139 | 252 | 204 | 214 | 279 | 328 | 280 | 297 | 257 | 336 | 18 | 26 | 94 | 243 | 59 | 51 | 464 | 155 | 322 | 55 | 139 | 151 | |
Labelled Cell Count | 490 | 180 | 353 | 277 | 269 | 335 | 408 | 352 | 359 | 316 | 398 | 25 | 32 | 117 | 311 | 72 | 71 | 464 | 155 | 322 | 55 | 139 | 151 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 5.3 | 5.9 | 5.8 | 5.6 | 4.9 | 4.6 | 4.9 | – | 4.4 | 4.7 | 4.7 | 6.2 | 6.7 | 7.3 | 5.5 | 6.2 | 6.6 | 5.6 | 7.4 | 8.0 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.6 | 1.4 | 1.6 | 1.5 | 1.3 | 1.5 | – | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.4 | 1.1 | 1.1 | 1.1 | 0.7 | 2.0 | 2.5 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.06 | -0.26 | -0.37 | -0.26 | – | -0.41 | -0.32 | -0.32 | 0.09 | 0.19 | 0.31 | -0.1 | 0.07 | 0.15 | -0.06 | 0.33 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.9 | 0.1 | -0.5 | 0 | 2.0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -1.6 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | 2.1 | 0.9 | 0.3 | 0.1 | – | -0.7 | -1.6 | -1.9 | 0 | 0 | 1.3 | 4.8 | 5.3 | 4.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Endosome | -1.7 | -1.7 | -10.9 | 0 | -9.0 | – | 0 | 0 | 0 | -2.7 | -3.1 | -1.4 | -1.7 | -3.0 | -1.3 |
Golgi | 0.3 | -0.2 | -3.4 | 0 | -3.8 | – | 0 | 0 | 0 | 0 | 0 | 4.8 | -2.1 | 0 | 0 |
Mitochondria | 4.8 | 2.5 | 12.5 | 0 | 10.8 | – | 0 | 0 | 0 | 3.4 | 2.5 | -0.9 | -5.5 | -3.8 | -2.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -3.1 | -4.4 | -6.1 | 0 | -5.6 | – | 0 | 0 | 0 | 0 | 0 | -3.2 | -4.7 | -3.5 | -3.3 |
SpindlePole | -4.1 | -4.3 | -5.8 | 0 | -3.3 | – | 0 | 0 | 0 | 0 | 0 | -2.8 | 0.8 | -1.2 | -0.1 |
Vacuole | 2.3 | 2.3 | 0.7 | 0.6 | -0.5 | – | -0.3 | 1.1 | 1.1 | 0 | 0 | 0 | 4.6 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3645 | 3.6255 | 3.5357 | 3.329 | 3.4021 | 3.6158 | 5.5265 | 6.7186 | 5.9873 | 5.9109 | 5.1468 | 5.8066 | 5.7698 | 7.0478 | 6.3973 | 7.0864 | 6.8103 | 6.643 |
Actin | 0.0031 | 0.011 | 0.0114 | 0.0017 | 0.0065 | 0.0179 | 0.0283 | 0.0175 | 0.0134 | 0.001 | 0.031 | 0.0262 | 0.0054 | 0.0116 | 0.0099 | 0.0015 | 0.0022 | 0.0073 |
Bud | 0.0003 | 0.0013 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0 | 0.0011 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 |
Bud Neck | 0.0003 | 0.0007 | 0.0064 | 0.0003 | 0.0004 | 0.0012 | 0.0004 | 0.0003 | 0.0008 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0003 | 0.0005 | 0.0037 | 0.0002 | 0.0003 |
Bud Periphery | 0.0002 | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0011 | 0.0003 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0002 |
Bud Site | 0.0003 | 0.0052 | 0.0015 | 0.0007 | 0.0004 | 0.0007 | 0.0007 | 0.001 | 0.0025 | 0.0001 | 0.0027 | 0.0004 | 0.0002 | 0.0012 | 0.0013 | 0.0003 | 0.0001 | 0.0001 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0083 | 0.0139 | 0.0025 | 0.0018 | 0.0019 | 0.0034 | 0.0092 | 0.0024 | 0.0063 | 0.0005 | 0.0056 | 0.0025 | 0.0029 | 0.0025 | 0.0022 | 0.001 | 0.0042 | 0.0018 |
Cytoplasmic Foci | 0.1684 | 0.2193 | 0.1863 | 0.162 | 0.1546 | 0.1379 | 0.1427 | 0.1908 | 0.1848 | 0.1022 | 0.1334 | 0.1404 | 0.1823 | 0.2052 | 0.1775 | 0.2166 | 0.2055 | 0.1633 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0018 | 0.0014 | 0.0002 | 0.0003 | 0.0006 | 0.0008 | 0.0012 | 0.0008 | 0.0019 | 0.0001 | 0.0005 | 0.0006 | 0.0004 | 0.0007 | 0.0003 | 0.0001 | 0.0005 | 0.0002 |
Endosome | 0.2376 | 0.2349 | 0.2303 | 0.2569 | 0.2908 | 0.1971 | 0.2562 | 0.2082 | 0.2718 | 0.2443 | 0.1575 | 0.1898 | 0.2469 | 0.236 | 0.2169 | 0.2247 | 0.2759 | 0.168 |
Golgi | 0.2909 | 0.3349 | 0.3775 | 0.4457 | 0.3894 | 0.4097 | 0.2754 | 0.3919 | 0.282 | 0.305 | 0.2137 | 0.3759 | 0.3512 | 0.3877 | 0.383 | 0.4335 | 0.3628 | 0.4232 |
Lipid Particles | 0.1064 | 0.0429 | 0.0401 | 0.0369 | 0.0253 | 0.0541 | 0.109 | 0.0451 | 0.0572 | 0.1532 | 0.1501 | 0.0986 | 0.0682 | 0.0274 | 0.0444 | 0.0121 | 0.0441 | 0.0553 |
Mitochondria | 0.0175 | 0.0275 | 0.0158 | 0.0355 | 0.0153 | 0.0362 | 0.0161 | 0.0214 | 0.0304 | 0.0072 | 0.0147 | 0.0396 | 0.0174 | 0.007 | 0.0196 | 0.0071 | 0.0064 | 0.0187 |
None | 0.0077 | 0.0026 | 0.0012 | 0.0001 | 0.0001 | 0.0027 | 0.0027 | 0.0004 | 0.0074 | 0 | 0.0866 | 0.007 | 0.0019 | 0.0038 | 0.0011 | 0.0001 | 0.0058 | 0.0002 |
Nuclear Periphery | 0.0038 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0046 | 0.0008 | 0.0008 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0001 |
Nucleolus | 0.0054 | 0.0015 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0039 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0018 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Peroxisomes | 0.139 | 0.093 | 0.1237 | 0.0527 | 0.1111 | 0.1327 | 0.1365 | 0.1148 | 0.1346 | 0.1831 | 0.1969 | 0.1125 | 0.1199 | 0.1118 | 0.14 | 0.097 | 0.0899 | 0.1593 |
Punctate Nuclear | 0.0017 | 0.0025 | 0.0006 | 0.0017 | 0.0002 | 0.0007 | 0.0108 | 0.0016 | 0.0008 | 0.0002 | 0.0015 | 0.0012 | 0.0004 | 0.0006 | 0.0005 | 0.0003 | 0.0003 | 0.0002 |
Vacuole | 0.0018 | 0.0034 | 0.001 | 0.0014 | 0.0014 | 0.0016 | 0.002 | 0.0011 | 0.0021 | 0.0014 | 0.0012 | 0.0014 | 0.0012 | 0.0017 | 0.001 | 0.0009 | 0.0009 | 0.0006 |
Vacuole Periphery | 0.0015 | 0.0017 | 0.0009 | 0.0015 | 0.0016 | 0.0014 | 0.0017 | 0.0011 | 0.002 | 0.0013 | 0.0011 | 0.0019 | 0.001 | 0.001 | 0.0009 | 0.0006 | 0.0008 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.7038 | 35.0564 | 28.4778 | 37.5682 | 23.014 | 24.3415 | 51.7691 | 53.2608 | 48.2422 | 46.3067 |
Translational Efficiency | 1.0222 | 1.0496 | 0.953 | 0.7417 | 0.8647 | 1.1418 | 0.7721 | 0.5826 | 0.601 | 0.5678 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
225 | 1239 | 193 | 1701 | 1826 | 2200 | 1383 | 688 | 2051 | 3439 | 1576 | 2389 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 862.94 | 773.45 | 1069.51 | 1015.66 | 798.42 | 813.59 | 983.56 | 1000.23 | 805.50 | 799.13 | 994.09 | 1011.22 |
Standard Deviation | 147.08 | 114.33 | 113.44 | 140.60 | 108.46 | 106.68 | 145.56 | 146.99 | 115.12 | 111.18 | 144.79 | 142.64 |
Intensity Change Log 2 | — | -0.157952 | 0.309618 | 0.235085 | — | 0.027154 | 0.300865 | 0.325112 | — | -0.066026 | 0.305418 | 0.279052 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003465 | 0.005317 | 0.016495 | 0.015281 | 0.002379 | 0.001240 | 0.023643 | 0.010824 | 0.002499 | 0.002709 | 0.022768 | 0.013998 |
Bud Neck | 0.006933 | 0.000600 | 0.002298 | 0.001723 | 0.000276 | 0.000844 | 0.000472 | 0.002654 | 0.001007 | 0.000756 | 0.000696 | 0.001991 |
Bud Site | 0.030810 | 0.001775 | 0.003400 | 0.015164 | 0.000999 | 0.001175 | 0.004194 | 0.016187 | 0.004270 | 0.001391 | 0.004096 | 0.015459 |
Cell Periphery | 0.000215 | 0.000065 | 0.000089 | 0.000067 | 0.000067 | 0.000040 | 0.000059 | 0.000146 | 0.000083 | 0.000049 | 0.000063 | 0.000090 |
Cytoplasm | 0.057745 | 0.004507 | 0.000726 | 0.010196 | 0.001187 | 0.004082 | 0.002333 | 0.009438 | 0.007392 | 0.004235 | 0.002136 | 0.009978 |
Cytoplasmic Foci | 0.212299* | 0.292506* | 0.374074* | 0.285756* | 0.215954* | 0.243938* | 0.320658* | 0.265161* | 0.215553* | 0.261436* | 0.327199* | 0.279825* |
Eisosomes | 0.000146 | 0.000109 | 0.000070 | 0.000048 | 0.000072 | 0.000096 | 0.000073 | 0.000070 | 0.000080 | 0.000101 | 0.000073 | 0.000054 |
Endoplasmic Reticulum | 0.002888 | 0.000412 | 0.001163 | 0.001159 | 0.000175 | 0.000294 | 0.001099 | 0.001255 | 0.000473 | 0.000336 | 0.001107 | 0.001187 |
Endosome | 0.290243* | 0.190829 | 0.203025* | 0.312356* | 0.197049 | 0.191111 | 0.280865* | 0.350329* | 0.207272* | 0.191009 | 0.271332* | 0.323292* |
Golgi | 0.052350 | 0.386514* | 0.232477* | 0.205705* | 0.511088* | 0.454792* | 0.288552* | 0.177172* | 0.460763* | 0.430193* | 0.281685* | 0.197488* |
Lipid Particles | 0.019724 | 0.036097 | 0.054440 | 0.010006 | 0.017005 | 0.026599 | 0.021785 | 0.012237 | 0.017304 | 0.030021 | 0.025784 | 0.010649 |
Mitochondria | 0.010214 | 0.018299 | 0.004382 | 0.005818 | 0.023904 | 0.034890 | 0.003336 | 0.010227 | 0.022402 | 0.028912 | 0.003464 | 0.007088 |
Mitotic Spindle | 0.019598 | 0.010740 | 0.038762 | 0.050245 | 0.001964 | 0.006846 | 0.018187 | 0.041172 | 0.003899 | 0.008249 | 0.020707 | 0.047632 |
None | 0.000091 | 0.000072 | 0.000022 | 0.000039 | 0.000095 | 0.000090 | 0.000021 | 0.000554 | 0.000095 | 0.000084 | 0.000021 | 0.000187 |
Nuclear Periphery | 0.000575 | 0.000092 | 0.000644 | 0.000359 | 0.000035 | 0.000104 | 0.000337 | 0.000361 | 0.000094 | 0.000100 | 0.000375 | 0.000360 |
Nuclear Periphery Foci | 0.003394 | 0.000966 | 0.004489 | 0.003634 | 0.000147 | 0.000369 | 0.002889 | 0.002593 | 0.000503 | 0.000584 | 0.003085 | 0.003334 |
Nucleolus | 0.002520 | 0.000125 | 0.000284 | 0.000123 | 0.000035 | 0.000101 | 0.000015 | 0.000253 | 0.000308 | 0.000110 | 0.000048 | 0.000160 |
Nucleus | 0.010639 | 0.000436 | 0.003448 | 0.000178 | 0.000046 | 0.000136 | 0.000148 | 0.002028 | 0.001208 | 0.000244 | 0.000552 | 0.000711 |
Peroxisomes | 0.052471 | 0.045177 | 0.036709 | 0.019199 | 0.022589 | 0.025086 | 0.014843 | 0.027448 | 0.025867 | 0.032324 | 0.017521 | 0.021574 |
Vacuole | 0.213221* | 0.003283 | 0.016456 | 0.055912 | 0.002655 | 0.004635 | 0.013638 | 0.060477 | 0.025755 | 0.004148 | 0.013983 | 0.057227 |
Vacuole Periphery | 0.010461 | 0.002079 | 0.006548 | 0.007032 | 0.002277 | 0.003533 | 0.002854 | 0.009412 | 0.003175 | 0.003009 | 0.003306 | 0.007717 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.54 | -2.76 | -5.46 | -4.70 | 0.28 | 3.45 | -8.15 | -3.58 | -4.10 | 3.73 | -0.50 | -8.58 | -7.30 | -7.16 | 3.21 |
Bud Neck | 4.51 | 2.60 | 3.55 | -6.58 | 0.34 | -3.93 | -2.95 | -4.45 | -3.36 | -4.07 | 1.31 | 1.34 | -4.38 | -6.23 | -5.80 |
Bud Site | 4.60 | 4.31 | 2.30 | -11.53 | -8.56 | -0.59 | -5.99 | -6.88 | -6.82 | -5.37 | 3.76 | 0.16 | -9.06 | -13.57 | -10.35 |
Cell Periphery | 3.63 | 2.98 | 3.58 | -0.40 | 1.72 | 2.06 | 0.54 | -2.07 | -2.86 | -2.35 | 2.70 | 1.55 | -0.58 | -3.54 | -2.37 |
Cytoplasm | 6.30 | 6.77 | 5.41 | -4.53 | -8.51 | -4.87 | -1.92 | -5.52 | -3.52 | -4.29 | 2.84 | 4.27 | -2.63 | -6.18 | -7.47 |
Cytoplasmic Foci | -6.41 | -8.55 | -5.51 | 1.80 | 5.96 | -5.99 | -16.47 | -6.17 | -2.47 | 6.70 | -10.86 | -18.49 | -12.01 | -3.03 | 8.09 |
Eisosomes | 1.91 | 4.32 | 6.03 | 4.90 | 2.69 | -2.59 | -0.25 | 0.13 | 2.16 | 0.30 | -2.53 | 1.19 | 4.47 | 6.11 | 3.61 |
Endoplasmic Reticulum | 2.95 | 1.98 | 1.90 | -2.36 | -0.03 | -1.81 | -7.10 | -4.08 | -3.58 | -0.61 | 1.22 | -4.39 | -2.96 | -3.65 | -0.33 |
Endosome | 4.86 | 3.77 | -1.44 | -16.17 | -9.07 | 1.04 | -12.56 | -15.18 | -15.80 | -6.33 | 2.93 | -10.19 | -18.12 | -22.96 | -7.12 |
Golgi | -34.29* | -10.86 | -16.48 | 23.21 | 2.06 | 7.60 | 29.17 | 37.36* | 30.87 | 12.32 | 4.36 | 23.15 | 37.99 | 40.48 | 13.19 |
Lipid Particles | -3.25 | -3.65 | 2.34 | 8.62 | 5.15 | -3.83 | -1.91 | 1.63 | 4.85 | 3.19 | -5.74 | -3.37 | 3.53 | 10.49 | 6.81 |
Mitochondria | -1.64 | 1.05 | 0.81 | 5.91 | -0.81 | -3.87 | 10.19 | 4.67 | 7.87 | -2.74 | -2.82 | 10.04 | 7.82 | 12.23 | -3.01 |
Mitotic Spindle | 1.63 | -1.92 | -5.19 | -11.23 | -1.72 | -5.16 | -7.02 | -7.41 | -6.44 | -4.10 | -4.27 | -7.17 | -15.23 | -13.70 | -7.91 |
None | 1.17 | 2.90 | 2.13 | 1.87 | -1.41 | 0.08 | 1.27 | -1.02 | -1.03 | -1.18 | 0.24 | 1.46 | -0.68 | -0.81 | -1.27 |
Nuclear Periphery | 4.80 | -0.18 | 1.68 | -4.52 | 1.22 | -2.38 | -2.10 | -3.25 | -2.56 | 0.13 | -0.09 | -2.15 | -5.19 | -5.13 | 0.26 |
Nuclear Periphery Foci | 3.12 | -0.43 | -0.34 | -3.86 | 0.23 | -2.38 | -4.84 | -3.36 | -3.05 | 0.35 | -0.39 | -4.70 | -5.27 | -5.17 | -0.53 |
Nucleolus | 1.92 | 1.77 | 1.91 | -0.25 | 0.61 | -2.12 | 1.20 | -3.23 | -2.23 | -3.49 | 1.45 | 1.82 | 0.98 | -1.86 | -3.05 |
Nucleus | 4.80 | 1.79 | 4.92 | 1.19 | 0.96 | -2.96 | -1.18 | -2.50 | -2.42 | -2.16 | 3.84 | 0.88 | 0.56 | -2.22 | -0.42 |
Peroxisomes | 0.75 | 1.36 | 4.25 | 7.31 | 2.24 | -1.15 | 3.41 | -1.60 | -0.80 | -4.11 | -2.94 | 3.45 | 2.02 | 5.40 | -1.79 |
Vacuole | 11.56 | 10.63 | 8.24 | -18.83 | -9.18 | -3.22 | -8.33 | -10.09 | -9.76 | -8.07 | 8.63 | 4.24 | -9.69 | -20.64 | -15.77 |
Vacuole Periphery | 3.07 | 1.04 | 1.16 | -7.49 | -0.18 | -2.58 | -1.48 | -4.65 | -3.70 | -4.21 | 0.24 | -0.37 | -6.54 | -6.92 | -6.09 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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