Standard name | |
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Human Ortholog | |
Description | Mitotic exit network regulator; forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 4 | 4 | 2 | 4 | 1 | 1 | 3 | 0 | 2 | 4 | 2 | 1 | 0 | 0 | 1 | 1 | 5 | 10 | 3 | 2 | 1 | 1 |
Bud | 4 | 13 | 4 | 7 | 4 | 12 | 13 | 23 | 29 | 23 | 47 | 27 | 1 | 0 | 0 | 2 | 6 | 7 | 2 | 7 | 2 | 1 | 4 | 5 |
Bud Neck | 11 | 26 | 22 | 15 | 7 | 26 | 20 | 39 | 22 | 24 | 37 | 31 | 9 | 6 | 5 | 6 | 4 | 2 | 2 | 4 | 1 | 1 | 0 | 1 |
Bud Site | 0 | 1 | 0 | 1 | 1 | 6 | 3 | 6 | 11 | 11 | 14 | 24 | 0 | 1 | 0 | 9 | 0 | 9 | – | – | – | – | – | – |
Cell Periphery | 0 | 5 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 2 |
Cytoplasm | 222 | 399 | 201 | 112 | 58 | 192 | 181 | 178 | 135 | 132 | 120 | 126 | 240 | 250 | 280 | 28 | 35 | 26 | 186 | 272 | 142 | 128 | 214 | 191 |
Endoplasmic Reticulum | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 22 | 6 | 1 | 0 | 0 | 3 | 1 | 2 | 2 | 4 | 4 | 0 | 2 | 7 | 20 | 20 | 25 | 20 | 21 | 24 | 14 | 2 | 2 | 2 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 3 | 3 | 1 | 0 | 2 | 0 |
Mitochondria | 10 | 17 | 0 | 4 | 1 | 27 | 13 | 22 | 94 | 56 | 104 | 79 | 0 | 1 | 1 | 12 | 6 | 5 | 21 | 45 | 22 | 7 | 7 | 5 |
Nucleus | 0 | 3 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 2 | 3 | 4 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 0 | 0 | 0 | 9 | 4 | 10 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 3 | 7 | 3 | 5 | 2 | 0 | 1 | 14 | 25 | 14 | 10 | 0 | 3 | 1 | 0 | 0 | 0 |
SpindlePole | 28 | 50 | 59 | 37 | 20 | 86 | 105 | 143 | 101 | 148 | 161 | 167 | 28 | 16 | 33 | 189 | 171 | 128 | 71 | 112 | 74 | 8 | 8 | 8 |
Vac/Vac Membrane | 1 | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 3 | 1 | 1 | 0 | 1 | 1 | 1 | 4 | 6 | 2 | 3 | 1 | 1 | 0 | 2 |
Unique Cell Count | 278 | 488 | 268 | 160 | 82 | 302 | 297 | 340 | 297 | 332 | 366 | 359 | 278 | 275 | 327 | 227 | 226 | 168 | 324 | 512 | 271 | 164 | 247 | 229 |
Labelled Cell Count | 303 | 526 | 294 | 181 | 93 | 362 | 342 | 421 | 407 | 407 | 503 | 468 | 284 | 287 | 355 | 303 | 275 | 228 | 324 | 512 | 271 | 164 | 247 | 229 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 3.3 | 4.6 | 4.1 | 3.7 | 3.8 | 3.9 | 4.3 | 3.1 | 3.5 | 3.3 | 3.4 | 4.7 | 4.7 | 4.4 | 5.0 | 6.0 | 6.1 | 4.7 | 5.2 | 5.4 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.0 | 1.0 | 0.7 | 1.1 | 1.2 | 1.7 | 0.8 | 0.8 | 1.1 | 1.0 | 0.9 | 1.4 | 1.1 | 1.0 | 1.3 | 1.3 | 1.6 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.32 | -0.28 | -0.23 | -0.09 | -0.59 | -0.38 | -0.48 | -0.45 | 0.04 | 0.04 | -0.07 | 0.12 | 0.38 | 0.41 | 0.03 | 0.19 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.365 | 3.4305 | 2.8073 | 2.5646 | 2.3889 | 2.7469 | 3.9915 | 4.0444 | 3.9821 | 3.6012 | 3.7444 | 4.1481 | 1.5943 | 1.4675 | 1.4139 | 1.6457 | 1.3799 | 1.5464 |
Actin | 0.0167 | 0.0003 | 0.0203 | 0.0142 | 0.0047 | 0.0096 | 0.0296 | 0.0075 | 0.0028 | 0.0007 | 0.0015 | 0.0034 | 0.0437 | 0.0004 | 0.0012 | 0.0088 | 0.0007 | 0.0058 |
Bud | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0025 | 0.0025 | 0.0006 | 0 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0002 |
Bud Neck | 0.0027 | 0.0008 | 0.0077 | 0.0948 | 0.0003 | 0.0031 | 0.0028 | 0.0015 | 0.0027 | 0.0108 | 0.0006 | 0.0027 | 0.0024 | 0.0003 | 0.005 | 0.0887 | 0.0004 | 0.001 |
Bud Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0028 | 0.004 | 0.0041 | 0.0007 | 0 | 0.0003 | 0.0008 | 0.0004 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0.0013 |
Bud Site | 0.0036 | 0.0013 | 0.0021 | 0.0076 | 0.0005 | 0.0003 | 0.0102 | 0.017 | 0.0043 | 0 | 0.0008 | 0.0005 | 0.0024 | 0.0025 | 0.0025 | 0.0007 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0194 | 0.041 | 0.0232 | 0.0196 | 0.0245 | 0.0407 | 0.0333 | 0.0616 | 0.0172 | 0.0092 | 0.0031 | 0.0343 | 0.0288 | 0.0298 | 0.0213 | 0.0158 | 0.0075 | 0.032 |
Cytoplasmic Foci | 0.3096 | 0.2612 | 0.3327 | 0.523 | 0.384 | 0.3478 | 0.2868 | 0.2029 | 0.3083 | 0.3158 | 0.6108 | 0.2918 | 0.2691 | 0.2002 | 0.2408 | 0.5103 | 0.4138 | 0.2228 |
Eisosomes | 0.0004 | 0.0006 | 0.0005 | 0.0014 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0008 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0007 | 0.0005 | 0.001 | 0.0016 | 0.0007 | 0.0014 | 0.0018 | 0.0019 | 0.0018 | 0.0002 | 0.001 | 0.0007 | 0.0022 | 0.0007 | 0.0006 | 0.0002 | 0.0003 | 0.0007 |
Endosome | 0.0145 | 0.0145 | 0.0097 | 0.0065 | 0.0581 | 0.0168 | 0.0067 | 0.0131 | 0.0216 | 0.0012 | 0.0134 | 0.0102 | 0.0167 | 0.0109 | 0.0129 | 0.0072 | 0.0184 | 0.0073 |
Golgi | 0.0052 | 0.0012 | 0.0026 | 0.0042 | 0.0066 | 0.0046 | 0.0031 | 0.0038 | 0.0022 | 0.0007 | 0.0041 | 0.0011 | 0.0043 | 0.0011 | 0.0013 | 0.0014 | 0.0042 | 0.0019 |
Lipid Particles | 0.0438 | 0.0175 | 0.0236 | 0.0157 | 0.0916 | 0.0125 | 0.0163 | 0.0068 | 0.0077 | 0.0126 | 0.042 | 0.0138 | 0.0202 | 0.0078 | 0.0145 | 0.0088 | 0.0993 | 0.013 |
Mitochondria | 0.0009 | 0.0002 | 0.0016 | 0.0024 | 0.003 | 0.0013 | 0.0015 | 0.0019 | 0.0006 | 0.0001 | 0.0003 | 0.0004 | 0.001 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0018 |
None | 0.3884 | 0.5486 | 0.4244 | 0.1101 | 0.3242 | 0.4066 | 0.4572 | 0.5614 | 0.4949 | 0.5281 | 0.1406 | 0.5093 | 0.5192 | 0.6745 | 0.5458 | 0.1637 | 0.1364 | 0.5418 |
Nuclear Periphery | 0.0056 | 0.0005 | 0.0007 | 0.0011 | 0.0085 | 0.0015 | 0.0215 | 0.001 | 0.0016 | 0.0002 | 0.0033 | 0.0013 | 0.0053 | 0.0008 | 0.0009 | 0.0007 | 0.0014 | 0.0022 |
Nucleolus | 0.0007 | 0.0138 | 0.0008 | 0.001 | 0.0008 | 0.0004 | 0.0011 | 0.0012 | 0.0011 | 0.0002 | 0.0006 | 0.0004 | 0.0009 | 0.001 | 0.0016 | 0.0014 | 0.0007 | 0.0005 |
Nucleus | 0.0005 | 0.0073 | 0.0007 | 0.0011 | 0.001 | 0.0011 | 0.0109 | 0.0014 | 0.001 | 0.0002 | 0.0007 | 0.0005 | 0.0016 | 0.001 | 0.0016 | 0.0009 | 0.0005 | 0.001 |
Peroxisomes | 0.056 | 0.0172 | 0.0325 | 0.0597 | 0.0629 | 0.0162 | 0.0221 | 0.0205 | 0.0141 | 0.0171 | 0.067 | 0.0111 | 0.0261 | 0.0151 | 0.018 | 0.0128 | 0.0673 | 0.0196 |
Punctate Nuclear | 0.13 | 0.0717 | 0.1147 | 0.134 | 0.0255 | 0.1312 | 0.086 | 0.0876 | 0.1153 | 0.1023 | 0.1091 | 0.1165 | 0.0537 | 0.0509 | 0.1295 | 0.1771 | 0.2482 | 0.1462 |
Vacuole | 0.0005 | 0.0013 | 0.0006 | 0.0005 | 0.0015 | 0.0008 | 0.0009 | 0.0015 | 0.0007 | 0 | 0.0005 | 0.0004 | 0.0007 | 0.0009 | 0.0008 | 0.0003 | 0.0003 | 0.0003 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0.0004 | 0.0005 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.1883 | 11.7808 | 3.1789 | 6.781 | 10.0966 | 5.9869 | 12.9944 | 6.5778 | 4.3873 | 10.5096 |
Translational Efficiency | 0.9823 | 0.5252 | 1.7342 | 0.6471 | 0.6058 | 1.0932 | 0.5841 | 0.9525 | 1.2995 | 0.7618 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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