Standard name
Human Ortholog
Description Mitotic exit network regulator; forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0 0 0.07 0.1 0.07 0.13 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0.05 0.08 0.09 0.09 0.09 0.07 0.11 0.07 0.07 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.05 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.82 0.75 0.7 0.71 0.64 0.61 0.52 0.45 0.4 0.33 0.35 0.86 0.91 0.86 0.12 0.15 0.15 0.58 0.53 0.52 0.78 0.87 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.11 0.12 0.07 0.05 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0 0.06 0.32 0.17 0.28 0.22 0 0 0 0.05 0 0 0.07 0.09 0.08 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.06 0.06 0 0 0 0 0 0
SpindlePole 0.1 0.1 0.22 0.23 0.24 0.28 0.35 0.42 0.34 0.45 0.44 0.47 0.1 0.06 0.1 0.83 0.76 0.76 0.22 0.22 0.28 0.05 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 4 4 2 4 1 1 3 0 2 4 2 1 0 0 1 1 5 10 3 2 1 1
Bud 4 13 4 7 4 12 13 23 29 23 47 27 1 0 0 2 6 7 2 7 2 1 4 5
Bud Neck 11 26 22 15 7 26 20 39 22 24 37 31 9 6 5 6 4 2 2 4 1 1 0 1
Bud Site 0 1 0 1 1 6 3 6 11 11 14 24 0 1 0 9 0 9 – – – – – –
Cell Periphery 0 5 1 0 0 1 0 0 0 0 1 1 1 1 0 0 0 0 0 3 1 0 1 2
Cytoplasm 222 399 201 112 58 192 181 178 135 132 120 126 240 250 280 28 35 26 186 272 142 128 214 191
Endoplasmic Reticulum 3 3 2 0 0 0 0 0 1 0 0 0 0 1 1 2 3 1 0 0 0 0 1 2
Endosome 22 6 1 0 0 3 1 2 2 4 4 0 2 7 20 20 25 20 21 24 14 2 2 2
Golgi 1 0 0 0 0 0 1 0 0 0 0 0 1 1 0 0 1 0 3 3 1 0 2 0
Mitochondria 10 17 0 4 1 27 13 22 94 56 104 79 0 1 1 12 6 5 21 45 22 7 7 5
Nucleus 0 3 0 0 0 1 1 3 1 2 3 4 0 0 0 0 1 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 4 2 0 0 0 9 4 10 0 0 0 1 0 0
Peroxisomes 0 1 0 0 0 2 1 3 7 3 5 2 0 1 14 25 14 10 0 3 1 0 0 0
SpindlePole 28 50 59 37 20 86 105 143 101 148 161 167 28 16 33 189 171 128 71 112 74 8 8 8
Vac/Vac Membrane 1 2 0 0 0 2 1 1 0 3 1 1 0 1 1 1 4 6 2 3 1 1 0 2
Unique Cell Count 278 488 268 160 82 302 297 340 297 332 366 359 278 275 327 227 226 168 324 512 271 164 247 229
Labelled Cell Count 303 526 294 181 93 362 342 421 407 407 503 468 284 287 355 303 275 228 324 512 271 164 247 229


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.3 4.6 4.1 3.7 3.8 3.9 4.3 3.1 3.5 3.3 3.4 4.7 4.7 4.4 5.0 6.0 6.1 4.7 5.2 5.4
Std Deviation (1e-4) 0.8 0.8 1.0 1.0 0.7 1.1 1.2 1.7 0.8 0.8 1.1 1.0 0.9 1.4 1.1 1.0 1.3 1.3 1.6 1.8 1.8
Intensity Change (Log2) – – – -0.17 -0.32 -0.28 -0.23 -0.09 -0.59 -0.38 -0.48 -0.45 0.04 0.04 -0.07 0.12 0.38 0.41 0.03 0.19 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.365 3.4305 2.8073 2.5646 2.3889 2.7469 3.9915 4.0444 3.9821 3.6012 3.7444 4.1481 1.5943 1.4675 1.4139 1.6457 1.3799 1.5464
Actin 0.0167 0.0003 0.0203 0.0142 0.0047 0.0096 0.0296 0.0075 0.0028 0.0007 0.0015 0.0034 0.0437 0.0004 0.0012 0.0088 0.0007 0.0058
Bud 0.0002 0.0001 0.0001 0.0002 0.0001 0.0005 0.0025 0.0025 0.0006 0 0.0002 0.0005 0.0002 0.0003 0.0003 0.0001 0 0.0002
Bud Neck 0.0027 0.0008 0.0077 0.0948 0.0003 0.0031 0.0028 0.0015 0.0027 0.0108 0.0006 0.0027 0.0024 0.0003 0.005 0.0887 0.0004 0.001
Bud Periphery 0.0002 0.0001 0.0001 0.0004 0.0003 0.0028 0.004 0.0041 0.0007 0 0.0003 0.0008 0.0004 0.0008 0.0004 0.0001 0.0001 0.0013
Bud Site 0.0036 0.0013 0.0021 0.0076 0.0005 0.0003 0.0102 0.017 0.0043 0 0.0008 0.0005 0.0024 0.0025 0.0025 0.0007 0.0001 0.0001
Cell Periphery 0.0002 0.0001 0.0002 0.0006 0.0001 0.0002 0.0004 0.0003 0.0001 0 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0194 0.041 0.0232 0.0196 0.0245 0.0407 0.0333 0.0616 0.0172 0.0092 0.0031 0.0343 0.0288 0.0298 0.0213 0.0158 0.0075 0.032
Cytoplasmic Foci 0.3096 0.2612 0.3327 0.523 0.384 0.3478 0.2868 0.2029 0.3083 0.3158 0.6108 0.2918 0.2691 0.2002 0.2408 0.5103 0.4138 0.2228
Eisosomes 0.0004 0.0006 0.0005 0.0014 0.0003 0.0001 0.0007 0.0002 0.0003 0.0003 0 0.0001 0.0008 0.0002 0.0002 0.0008 0.0001 0.0001
Endoplasmic Reticulum 0.0007 0.0005 0.001 0.0016 0.0007 0.0014 0.0018 0.0019 0.0018 0.0002 0.001 0.0007 0.0022 0.0007 0.0006 0.0002 0.0003 0.0007
Endosome 0.0145 0.0145 0.0097 0.0065 0.0581 0.0168 0.0067 0.0131 0.0216 0.0012 0.0134 0.0102 0.0167 0.0109 0.0129 0.0072 0.0184 0.0073
Golgi 0.0052 0.0012 0.0026 0.0042 0.0066 0.0046 0.0031 0.0038 0.0022 0.0007 0.0041 0.0011 0.0043 0.0011 0.0013 0.0014 0.0042 0.0019
Lipid Particles 0.0438 0.0175 0.0236 0.0157 0.0916 0.0125 0.0163 0.0068 0.0077 0.0126 0.042 0.0138 0.0202 0.0078 0.0145 0.0088 0.0993 0.013
Mitochondria 0.0009 0.0002 0.0016 0.0024 0.003 0.0013 0.0015 0.0019 0.0006 0.0001 0.0003 0.0004 0.001 0.0004 0.0005 0.0003 0.0003 0.0018
None 0.3884 0.5486 0.4244 0.1101 0.3242 0.4066 0.4572 0.5614 0.4949 0.5281 0.1406 0.5093 0.5192 0.6745 0.5458 0.1637 0.1364 0.5418
Nuclear Periphery 0.0056 0.0005 0.0007 0.0011 0.0085 0.0015 0.0215 0.001 0.0016 0.0002 0.0033 0.0013 0.0053 0.0008 0.0009 0.0007 0.0014 0.0022
Nucleolus 0.0007 0.0138 0.0008 0.001 0.0008 0.0004 0.0011 0.0012 0.0011 0.0002 0.0006 0.0004 0.0009 0.001 0.0016 0.0014 0.0007 0.0005
Nucleus 0.0005 0.0073 0.0007 0.0011 0.001 0.0011 0.0109 0.0014 0.001 0.0002 0.0007 0.0005 0.0016 0.001 0.0016 0.0009 0.0005 0.001
Peroxisomes 0.056 0.0172 0.0325 0.0597 0.0629 0.0162 0.0221 0.0205 0.0141 0.0171 0.067 0.0111 0.0261 0.0151 0.018 0.0128 0.0673 0.0196
Punctate Nuclear 0.13 0.0717 0.1147 0.134 0.0255 0.1312 0.086 0.0876 0.1153 0.1023 0.1091 0.1165 0.0537 0.0509 0.1295 0.1771 0.2482 0.1462
Vacuole 0.0005 0.0013 0.0006 0.0005 0.0015 0.0008 0.0009 0.0015 0.0007 0 0.0005 0.0004 0.0007 0.0009 0.0008 0.0003 0.0003 0.0003
Vacuole Periphery 0.0002 0.0001 0.0001 0.0002 0.0006 0.0004 0.0005 0.0005 0.0002 0 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.1883 11.7808 3.1789 6.781 10.0966 5.9869 12.9944 6.5778 4.3873 10.5096
Translational Efficiency 0.9823 0.5252 1.7342 0.6471 0.6058 1.0932 0.5841 0.9525 1.2995 0.7618

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1603 1764 214 114 2189 1432 1031 1886 3792 3196 1245 2000

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 689.35 707.54 842.06 904.90 640.01 771.91 807.90 843.37 660.87 736.38 813.77 846.88
Standard Deviation 78.04 82.43 92.72 103.14 72.70 91.08 96.07 112.96 78.86 92.15 96.36 113.33
Intensity Change Log 2 — 0.037575 0.288686 0.392522 — 0.270338 0.336082 0.398071 — 0.154329 0.311699 0.395196

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000148 0.000643 0.000893 0.000485 0.000089 0.001045 0.000488 0.000668 0.000114 0.000823 0.000557 0.000657
Bud Neck 0.012197 0.031853 0.006033 0.003074 0.016808 0.051278 0.009217 0.013054 0.014858 0.040557 0.008670 0.012485
Bud Site 0.003730 0.014601 0.012048 0.024918 0.004254 0.021881 0.030729 0.055250 0.004033 0.017863 0.027518 0.053521
Cell Periphery 0.000110 0.000117 0.000263 0.000125 0.000133 0.000218 0.000201 0.000117 0.000123 0.000162 0.000211 0.000117
Cytoplasm 0.244627* 0.293784* 0.117167* 0.069548 0.338951* 0.289064* 0.115737* 0.101233* 0.299077* 0.291669* 0.115983* 0.099427
Cytoplasmic Foci 0.525100* 0.394657* 0.098229 0.124113 0.414650* 0.387870* 0.108094 0.129072 0.461341* 0.391616* 0.106398 0.128789
Eisosomes 0.000156 0.000170 0.000174 0.000045 0.000186 0.000268 0.000061 0.000032 0.000173 0.000214 0.000081 0.000033
Endoplasmic Reticulum 0.000525 0.000792 0.005053 0.000927 0.001006 0.000415 0.000951 0.000425 0.000803 0.000623 0.001656 0.000454
Endosome 0.024563 0.032431 0.021770 0.014555 0.017745 0.030826 0.016064 0.022047 0.020627 0.031712 0.017045 0.021620
Golgi 0.008309 0.015998 0.001078 0.002301 0.006230 0.014056 0.003719 0.003976 0.007109 0.015128 0.003265 0.003880
Lipid Particles 0.038279 0.014703 0.014954 0.008075 0.036614 0.017348 0.005690 0.004336 0.037318 0.015888 0.007283 0.004549
Mitochondria 0.005092 0.021352 0.000549 0.000906 0.005789 0.021784 0.000794 0.002156 0.005494 0.021546 0.000752 0.002085
Mitotic Spindle 0.017128 0.023476 0.228816* 0.600079* 0.006054 0.009835 0.525438* 0.576527* 0.010735 0.017364 0.474452* 0.577870*
None 0.006448 0.003191 0.004660 0.000453 0.007872 0.004250 0.008368 0.001859 0.007270 0.003665 0.007731 0.001779
Nuclear Periphery 0.000901 0.000406 0.001928 0.000758 0.000660 0.000329 0.000890 0.000517 0.000762 0.000372 0.001069 0.000530
Nuclear Periphery Foci 0.003543 0.001405 0.050772 0.054567 0.001658 0.000741 0.031512 0.007518 0.002455 0.001107 0.034822 0.010200
Nucleolus 0.004365 0.002835 0.014689 0.010366 0.003794 0.002823 0.013237 0.005711 0.004035 0.002830 0.013487 0.005977
Nucleus 0.051205 0.061507 0.317178* 0.025159 0.100084 0.064284 0.077299 0.014434 0.079421 0.062751 0.118531 0.015045
Peroxisomes 0.038328 0.041059 0.026357 0.027352 0.019299 0.041530 0.033710 0.038957 0.027343 0.041270 0.032447 0.038296
Vacuole 0.014711 0.042921 0.076886 0.031271 0.017444 0.038231 0.017397 0.020310 0.016288 0.040819 0.027622 0.020935
Vacuole Periphery 0.000534 0.002101 0.000502 0.000923 0.000682 0.001925 0.000403 0.001802 0.000620 0.002022 0.000420 0.001752

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.66 -7.16 -5.31 -1.69 1.47 -2.29 -11.09 -7.83 0.84 -1.20 -3.69 -12.72 -8.14 0.58 -0.05
Bud Neck -10.64 -0.39 5.21 13.37 3.05 -11.26 5.57 3.87 13.04 -1.93 -14.81 4.29 2.84 15.90 -1.37
Bud Site -12.32 -6.20 -5.16 -3.88 -2.76 -8.24 -11.31 -18.15 -8.75 -5.77 -12.75 -12.75 -19.12 -12.34 -6.68
Cell Periphery -0.95 -6.55 -1.01 -0.84 2.13 -3.92 -2.22 2.38 4.95 3.79 -3.64 -4.78 1.33 4.06 5.07
Cytoplasm -7.67 13.37 7.35 11.26 -0.68 3.64 31.55 31.02 23.29 -2.35 -1.62 32.19 30.13 30.31 -2.55
Cytoplasmic Foci 15.94 31.91 23.27 15.66 0.49 4.38 35.53 38.23 29.60 -1.22 12.97 49.56 52.29 39.19 -1.33
Eisosomes -1.26 -5.00 2.12 2.50 5.51 -4.97 8.68 16.74 14.30 10.64 -3.40 5.21 19.78 12.38 13.34
Endoplasmic Reticulum -3.44 -7.59 -3.54 -2.72 5.05 2.77 -2.81 2.20 -1.76 8.52 0.99 -8.02 1.14 0.32 10.11
Endosome -4.59 -2.33 1.17 3.46 2.60 -6.53 -4.15 -0.81 5.46 3.21 -8.32 -3.97 0.72 7.53 4.02
Golgi -6.30 7.28 6.06 10.76 -0.01 -5.31 3.34 4.11 7.58 0.43 -8.48 5.86 6.72 12.91 0.25
Lipid Particles 9.45 9.13 12.87 5.91 4.29 8.04 14.95 16.18 9.14 2.92 12.55 18.99 21.44 12.16 4.70
Mitochondria -9.37 8.34 8.12 12.07 1.40 -8.09 8.46 6.87 10.79 -2.51 -12.17 11.51 8.74 15.73 -2.37
Mitotic Spindle -1.09 -9.73 -12.36 -12.27 -5.45 -2.15 -39.01* -62.32* -61.08* -4.58 -3.14 -38.40 -62.49 -61.37* -7.53
None 5.78 3.73 6.32 2.55 3.45 3.35 2.07 6.69 3.46 3.78 6.26 2.77 8.93 3.90 4.22
Nuclear Periphery 0.89 -3.39 -0.57 -3.45 4.67 2.48 -3.96 -0.39 -5.67 5.53 1.71 -3.46 -0.01 -6.22 7.77
Nuclear Periphery Foci 4.95 -7.85 -3.62 -3.99 2.36 3.10 -14.56 -8.83 -10.04 10.48 5.36 -16.25 -8.63 -10.39 11.59
Nucleolus 4.19 -6.53 0.25 -1.02 6.10 3.71 -9.41 -3.85 -5.96 7.47 5.24 -11.01 -2.96 -5.63 9.23
Nucleus -4.99 -7.79 1.93 3.96 8.03 7.48 7.38 24.21 14.31 7.53 3.95 -1.02 22.92 18.40 10.98
Peroxisomes -1.12 -0.68 0.18 0.58 0.61 -8.66 -4.39 -7.52 1.71 -1.66 -7.70 -2.32 -4.23 1.99 -1.09
Vacuole -13.80 -6.31 -4.47 -2.68 -1.30 -9.61 -1.64 -4.23 4.08 -1.67 -16.45 -5.28 -6.45 4.19 -0.41
Vacuole Periphery -8.92 -0.25 0.09 5.30 0.16 -5.76 5.75 -1.18 2.95 -2.77 -10.15 5.72 -1.48 3.96 -2.65
Standard name
Human Ortholog
Description Mitotic exit network regulator; forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage
Localization
Cell Percentages cytoplasm (19%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bub2

Bub2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bub2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available